Abstract:
:Establishment of symbiosis between legumes and rhizobia requires bacterial Nod factors (NFs). The concentration of these lipochitooligosaccharides in the rhizosphere is influenced by plant enzymes. NFs induce on pea (Pisum sativum) a particular extracellular NF hydrolase that releases lipodisaccharides from NFs from Sinorhizobium meliloti. Here, we investigated the ability of non-nodulating pea mutants to respond to NodRlv factors (NFs from Rhizobium leguminosarum bv viciae) with enhanced NF hydrolase activity. Mutants defective in the symbiotic genes sym10, sym8, sym19, and sym9/sym30 did not exhibit any stimulation of the NF hydrolase, indicating that the enzyme is induced via an NF signal transduction pathway that includes calcium spiking (transient increases in intracellular Ca(2+) levels). Interestingly, the NF hydrolase activity in these sym mutants was even lower than in wild-type peas, which were not pretreated with NodRlv factors. Activation of the NF hydrolase in wild-type plants was a specific response to NodRlv factors. The induction of the NF hydrolase was blocked by alpha-amanitin, cycloheximide, tunicamycin, EGTA, U73122, and calyculin A. Inhibitory effects, albeit weaker, were also found for brefeldin A, BHQ and ethephon. In addition to this NF hydrolase, NFs and stress-related signals (ethylene and salicylic acid) stimulated a pea chitinase that released lipotrisaccharides from pentameric NFs from S. meliloti. NodRlv factors failed to stimulate the chitinase in mutants defective in the sym10 and sym8 genes, whereas other mutants (e.g. mutated in the sym19 gene) retained their ability to increase the chitinase activity. These findings indicate that calcium spiking is not implicated in stimulation of the chitinase. We suggest that downstream of Sym8, a stress-related signal transduction pathway branches off from the NF signal transduction pathway.
journal_name
Plant Physioljournal_title
Plant physiologyauthors
Ovtsyna AO,Dolgikh EA,Kilanova AS,Tsyganov VE,Borisov AY,Tikhonovich IA,Staehelin Cdoi
10.1104/pp.105.061705subject
Has Abstractpub_date
2005-10-01 00:00:00pages
1051-64issue
2eissn
0032-0889issn
1532-2548pii
pp.105.061705journal_volume
139pub_type
杂志文章相关文献
PLANT PHYSIOLOGY文献大全abstract::The emergence of sterile individuals in the hybrid backcross progeny of wild and cultivated rice limits the use of wild rice alleles for improving cultivated rice, but the molecular mechanisms underlying this sterility remain unclear. Here, we identified the semisterile introgression line YIL42, derived from a cross b...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.18.01374
更新日期:2019-05-01 00:00:00
abstract::Young plants of five Nicotiana tabacum L. genotypes were examined for activity of nicotine biosynthetic enzymes. Genotypes near isogenic except at two loci each with two alleles controlling nicotine level were used in a comparison of the four homozygous allelic combinations producing high, high intermediate, low inter...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.64.2.236
更新日期:1979-08-01 00:00:00
abstract::Extracellular adhesives from the diatoms Achnanthes longipes, Amphora coffeaeformis, Cymbella cistula, and Cymbella mexicana were characterized by monosaccharide and methylation analysis, lectin-fluorescein isothiocyanate localization, and cytochemical staining. Polysaccharide was the major component of adhesives form...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.113.4.1059
更新日期:1997-04-01 00:00:00
abstract::An arginase, purified from the leaf of the jack bean, Canavalia ensiformis, can effectively hydrolyze both l- and d-arginine. Arginases, examined from a number of other plant and animal sources, exhibit marked substrate stereospecificity and fail to catabolize d-arginine. In order to provide essential nitrogen, jack b...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.94.1.67
更新日期:1990-09-01 00:00:00
abstract::Isolated cells obtained by enzymic digestion of young primary leaves of cold-hardened, dark-grown Kharkov winter wheat (Triticum aestivum L.) were exposed to various low temperature stresses. The initial uptake of (86)Rb was generally decreased by increasing concentrations of Ca(2+), but after longer periods of incuba...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.78.3.484
更新日期:1985-07-01 00:00:00
abstract::A comparison of barley (Hordeum vulgare L.) leaves was made between the cytosolic content of amino acids and sucrose as determined by subcellular fractionation and the corresponding concentration in phloem sap, which was collected continuously for up to 6 days from severed aphid stylets. Because amino acids were found...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.99.3.996
更新日期:1992-07-01 00:00:00
abstract::The Golgi apparatus in plant cells consists of a large number of independent Golgi stack/trans-Golgi network/Golgi matrix units that appear to be randomly distributed throughout the cytoplasm. To study the dynamic behavior of these Golgi units in living plant cells, we have cloned a cDNA from soybean (Glycine max), Gm...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.121.4.1127
更新日期:1999-12-01 00:00:00
abstract::The eukaryotic genome is duplicated exactly once per cell division cycle. A strategy that limits every replication origin to a single initiation event is tightly regulated by a multiprotein complex, which involves at least 20 protein factors. A key player in this regulation is the evolutionary conserved hexameric MCM2...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.105.074294
更新日期:2006-02-01 00:00:00
abstract::Auxin-binding protein 1 subsp. mays (ABP1) has been suggested as a receptor mediating auxin-induced cell expansion and differentiation. In maize (Zea mays), ABP1 is encoded by a single gene, Abp1. The TATA and CAAT promoter elements as well as the transcriptional start site were previously identified and all were foun...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.124.1.369
更新日期:2000-09-01 00:00:00
abstract::The concentrations of indole-3-acetic acid (IAA), naphthaleneacetic acid (NAA) and 2,4-dichlorophenoxyacetic acid (2,4-D) were followed for 35 days in cell-free liquid medium containing 100, 50, or 0% Murashige-Skoog (MS) salt base. Although the concentrations of NAA or 2,4-D remained constant the level of IAA decreas...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.81.3.934
更新日期:1986-07-01 00:00:00
abstract::Rates of assimilation and transpiration in Eucalyptus pauciflora Sieb. ex Spreng were measured at various ambient partial pressures of CO(2) and various irradiances and were used to estimate leaf conductance and intercellular partial pressure of CO(2). The responses of leaf conductance and rate of assimilation to chan...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.62.4.670
更新日期:1978-10-01 00:00:00
abstract::This study was designed to address the question: How specific for double bond position and conformation are plant enzymes that act on oleoyl-acyl carrier protein (ACP)? Octadecenoyl-ACPs with cis double bonds at positions [delta]6, [delta]7, [delta]8, [delta]9, [delta]10, [delta]11, or [delta]12 and elaidyl (18:1[delt...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.104.3.839
更新日期:1994-03-01 00:00:00
abstract::Plants respond to low carbon supply by massive reprogramming of the transcriptome and metabolome. We show here that the carbon starvation-induced NAC (for NO APICAL MERISTEM/ARABIDOPSIS TRANSCRIPTION ACTIVATION FACTOR/CUP-SHAPED COTYLEDON) transcription factor Arabidopsis (Arabidopsis thaliana) Transcription Activatio...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.15.00917
更新日期:2015-09-01 00:00:00
abstract::The circadian RNA-binding protein CHLAMY1 from the green alga Chlamydomonas reinhardtii consists of two subunits named C1 and C3. Changes in the C1 level cause arrhythmicity of the phototaxis rhythm, while alterations in the level of C3 lead to acrophase shifts. Thus, CHLAMY1 is involved in maintaining period and phas...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.108.118570
更新日期:2008-08-01 00:00:00
abstract::An apparatus for measuring simultaneously the surface electrical potentials along epicotyl was developed to study the largely bending situation by gravity. Potentials increased on the upper side and decreased on the lower side after the horizontal placement. The time course of electrical changes consisted of two compo...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.97.1.193
更新日期:1991-09-01 00:00:00
abstract::Numerous studies have demonstrated a rapid increase in the respiration rate during aging of slices of tuber and storage roots. To determine the molecular mechanisms of this phenomenon, the role of enzyme binding to the subcellular particulate fraction has been assessed in carrot (Daucus carota L.) and sugar beet (Beta...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.86.2.348
更新日期:1988-02-01 00:00:00
abstract::Light induces beta-carotene synthesis in streptomycin-bleached Euglena gracilis Z. Light-adapted, chemostat-grown cells have up to 10-fold as much beta-carotene and 25% more protein than similarly grown, dark-adapted cells. Carotenogenesis does not occur under anaerobic conditions or in the presence of diphenylamine, ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.46.5.685
更新日期:1970-11-01 00:00:00
abstract::Two maize (Zea mays) cyclin-dependent kinase (CDK) inhibitors, Zeama;KRP;1 and Zeama;KRP;2, were characterized and shown to be expressed in developing endosperm. Similar to the CDK inhibitors in Arabidopsis (Arabidopsis thaliana) and tobacco (Nicotiana tabacum), the maize proteins contain a carboxy-terminal region rel...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.105.063917
更新日期:2005-08-01 00:00:00
abstract::When (32)P(1) was supplied as a 15-minute pulse to normal Spirodela oligorrhiza plants, the first phospholipid to become fully labeled was phosphatidic acid. Phosphatidyl glycerol reached maximum labeling before the other major phospholipids. In phosphorus-deficient plants, however, phosphatidyl glycerol became labele...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.49.5.740
更新日期:1972-05-01 00:00:00
abstract::Low red light/far-red light ratio (R:FR) serves as an indicator of impending competition and has been demonstrated to suppress branch development. The regulation of Arabidopsis (Arabidopsis thaliana) rosette bud outgrowth by the R:FR and the associated mechanisms were investigated at several levels. Growth under low R...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.113.221895
更新日期:2013-10-01 00:00:00
abstract::Arbuscular mycorrhiza (AM) is a mutual symbiosis that involves a complex symbiotic interface over which nutrients are exchanged between the plant host and the AM fungus. Dozens of genes in the host are required for the establishment and functioning of the interaction, among them nutrient transporters that mediate the ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.15.00310
更新日期:2015-07-01 00:00:00
abstract::Intact peroxisomes were prepared from green leaves of a number of C(3) species, both monocots and dicots. A protoplast extract from which chloroplasts have been removed by a 1-minute 10,000g centrifugation was applied to a step gradient of 5, 15, 30, and 45% Percoll containing 0.5 molar sucrose, 0.1% BSA, and 25 milli...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.70.4.1213
更新日期:1982-10-01 00:00:00
abstract::The Ussing-Theorell equation, which provides a fundamental test for the independent passive movement of ions under conditions of nonequilibrium, has been used to assess the active and passive components of K(+) uptake by segments of pea epicotyl (Pisum sativum L. cultivar Alaska), incubated for 24 hours in both 1-fold...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.45.2.133
更新日期:1970-02-01 00:00:00
abstract::Experiments were designed to test the hypothesis that the labeled products recovered from plant tissue incubated with [(14)C]GA(12)-7-aldehyde ([(14)C]GA(12)ald) would serve as appropriate [(14)C]markers for the recovery of naturally-occurring gibberellins (GAs). The [(14)C]GA(12)ald (about 200 millicuries per millimo...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.81.4.984
更新日期:1986-08-01 00:00:00
abstract::Indole-3-acetyl-myo-inositol galactoside labeled with 3H in the indole and 14C in the galactose moieties was applied to kernels of 5 day old germinating seedlings of Zea mays. Indole-3-acetyl-myo-inositol galactoside was not transported into either the shoot or root tissue as the intact molecule but was instead hydro...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.80.4.961
更新日期:1986-01-01 00:00:00
abstract::Compounds with planar triple ring systems such as acridine orange, 9-amino acridine, 9-amino-1,2,3,4-tetrahydroacridine (tacrine), 6,9-diamino-2-ethoxyacridine lactate monohydrate (DE-acridine), 6-chloro-9-(3'-diethylamino-2'-hydroxypropylamino) -2-methoxyacridine.2 HCl (CDM-acridine), quinacrine, 6-chloro-9-(4'-dieth...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.47.3.346
更新日期:1971-03-01 00:00:00
abstract::The promoter of phytoene synthase, the first specific enzyme of carotenoid biosynthesis, shows two main regulatory regions: a G-box-containing region located near the TATA box, and a TATA box distal region containing the cis-acting element ATCTA, which mediates strong basal promoter activity. This second element was a...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.107.104828
更新日期:2007-11-01 00:00:00
abstract::Large changes in indole-3-acetic acid (IAA) levels occur during growth of Lemna gibba G-3 in sterile culture. The levels of IAA were measured in plants during a 45 day growth cycle using HPLC and isotope dilution analysis followed by selected ion current monitoring GC-MS analysis with (13)C(6)-IAA as the internal stan...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.86.2.522
更新日期:1988-02-01 00:00:00
abstract::Suspension cultures of carrot (Daucus carota, line C1), tobacco (Nicotiana tabacum, line TX1), and Nicotiana plumbaginifolia (line NP) were frozen under controlled conditions with trehalose as the sole cryoprotectant. Maximal post-thaw viability (71-74%), measured by phenosafranin dye exclusion, was obtained with the ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.78.2.430
更新日期:1985-06-01 00:00:00
abstract::Crude plasma membranes of corn (Zea mays L.) roots were obtained according to MI De Michelis and RM Spanswick (1986 Plant Physiol 81: 542-547). This preparation, which contained tightly sealed vesicles displaying Mg-ATP dependent H(+)-transport, was purified by phase partitioning. The percentage of inside-out vesicles...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.87.2.491
更新日期:1988-06-01 00:00:00