Isolation and purification of intact peroxisomes from green leaf tissue.

Abstract:

:Intact peroxisomes were prepared from green leaves of a number of C(3) species, both monocots and dicots. A protoplast extract from which chloroplasts have been removed by a 1-minute 10,000g centrifugation was applied to a step gradient of 5, 15, 30, and 45% Percoll containing 0.5 molar sucrose, 0.1% BSA, and 25 millimolar Hepes-KOH (pH 7.5). After centrifugation, a peroxisomal fraction with low contamination by chloroplastic and mitochondrial markers was recovered from the 30/45% Percoll interface. This fraction was passed through a Sepharose 2B column to remove the Percoll which resulted in a peroxisomal preparation exhibiting high intactness (estimated from enzyme latency) and stability.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Schmitt MR,Edwards GE

doi

10.1104/pp.70.4.1213

subject

Has Abstract

pub_date

1982-10-01 00:00:00

pages

1213-7

issue

4

eissn

0032-0889

issn

1532-2548

journal_volume

70

pub_type

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