Abstract:
:Intact peroxisomes were prepared from green leaves of a number of C(3) species, both monocots and dicots. A protoplast extract from which chloroplasts have been removed by a 1-minute 10,000g centrifugation was applied to a step gradient of 5, 15, 30, and 45% Percoll containing 0.5 molar sucrose, 0.1% BSA, and 25 millimolar Hepes-KOH (pH 7.5). After centrifugation, a peroxisomal fraction with low contamination by chloroplastic and mitochondrial markers was recovered from the 30/45% Percoll interface. This fraction was passed through a Sepharose 2B column to remove the Percoll which resulted in a peroxisomal preparation exhibiting high intactness (estimated from enzyme latency) and stability.
journal_name
Plant Physioljournal_title
Plant physiologyauthors
Schmitt MR,Edwards GEdoi
10.1104/pp.70.4.1213subject
Has Abstractpub_date
1982-10-01 00:00:00pages
1213-7issue
4eissn
0032-0889issn
1532-2548journal_volume
70pub_type
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