Responses of superoxide dismutase and glutathione reductase activities in cotton leaf tissue exposed to an atmosphere enriched in oxygen.

Abstract:

:Responses of superoxide dismutase (EC 1.15.1.1) and glutathione reductase (EC 1.6.4.2) activities were evaluated in leaf tissue from intact cotton plants (Cotton Branch 1697) which were exposed to 75% O(2), 350 microliters per liter CO(2) for 48 hours. Soluble protein was extracted from O(2)-treated and control tissue, and enzyme levels were determined. Superoxide dismutase activity in cotton leaf tissue was high (26 units per milligram protein) under normal conditions of 21% O(2), saturating light, and limiting CO(2), and neither qualitative nor quantitative differences in the cyanide-sensitive or -insensitive forms of the enzyme occurred in response to hyperoxic conditions. Glutathione reductase activity, however, was 2- to 3-fold higher in extracts from tissue exposed to 75% O(2). No increase in activity was observed for the peroxisomal enzymes, glycolate oxidase (EC 1.1.3.1) and catalase (EC 1.11.1.6). Results are consistent with an integrated pathway involving superoxide dismutase and glutathione reductase for protection of sensitive leaf components against detrimental effects of intermediate reduction products of O(2).

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Foster JG,Hess JL

doi

10.1104/pp.66.3.482

subject

Has Abstract

pub_date

1980-09-01 00:00:00

pages

482-7

issue

3

eissn

0032-0889

issn

1532-2548

journal_volume

66

pub_type

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