Molecular and biochemical characterization of the selenocysteine Se-methyltransferase gene and Se-methylselenocysteine synthesis in broccoli.

abstract：:Auxin biosynthesis was analyzed in a maize (Zea mays) kernel culture system in which the seeds develop under physiological conditions similar to the in vivo situation. This system was modified for precursor feeding experiments. Tryptophan (Trp) is efficiently incorporated into indole-3-acetic acid (IAA) with retention...

journal_title：Plant physiology

authors： Glawischnig E,Tomas A,Eisenreich W,Spiteller P,Bacher A,Gierl A

更新日期：2000-07-01 00:00:00

abstract：:The metabolism of soluble nucleotides was investigated in wheat (Triticum aestivum var. Olympic) aleurone layers treated with gibberellic acid. Whereas nucleotide levels were relatively unaffected by the treatment, a transient increase was observed in the incorporation of (32)P. The effect was maximal 30 to 60 minutes...

journal_title：Plant physiology

authors： Collins GG,Jenner CF,Paleg LG

更新日期：1972-03-01 00:00:00

abstract：:Of the eight intermediates associated with the two pathways of UDP-d-glucuronic acid biosynthesis found in plants, only d-glucuronic acid inhibited myo-inositol 1-phosphate synthase (EC 5.5.1.4), formerly referred to as d-glucose 6-phosphate cycloaldolase. Inhibition was competitive. An attempt to demonstrate over-all...

journal_title：Plant physiology

authors： Loewus MW,Loewus F

更新日期：1974-09-01 00:00:00

abstract：:Sucrose synthase (SUS) is phosphorylated on a major, amino-terminal site located at Ser-15 (S15) in the maize (Zea mays) SUS1 protein. Site- and phospho-specific antibodies against a phosphorylated S15 (pS15) peptide allowed direct analysis of S15 phosphorylation in relation to membrane association. Immunoblots of the...

journal_title：Plant physiology

authors： Hardin SC,Winter H,Huber SC

更新日期：2004-04-01 00:00:00

abstract：:DNA polymerase zeta catalytic subunit REV3 is known to play an important role in the repair of DNA damage induced by cross-linking and methylating agents. Here, we demonstrate that in Arabidopsis (Arabidopsis thaliana), the basic polymerase activity of REV3 is essential for resistance protection against these differen...

journal_title：Plant physiology

authors： Kobbe S,Trapp O,Knoll A,Manuss A,Puchta H

更新日期：2015-12-01 00:00:00

abstract：:The nature of the association between nitrate reductase (NR) and membranes was examined. Nitrate reductase activity (NRA) associated with the microsomal fraction of barley (Hordeum vulgare L.) roots amounted to 0.6 to 0.8% of soluble NRA following sonication in the presence of 250 mM KI and repeated osmotic shock. T...

journal_title：Plant physiology

authors： Meyerhoff PA,Fox TC,Travis RL,Huffaker RC

更新日期：1994-01-01 00:00:00

abstract：:Plants possess many metabolic genes for the production of a wide variety of phytochemicals in a tissue-specific manner. However, the metabolic systems behind the diversity and tissue-dependent regulation still remain unknown due to incomplete characterization of phytochemicals produced in a single plant species. Thus,...

journal_title：Plant physiology

authors： Matsuda F,Hirai MY,Sasaki E,Akiyama K,Yonekura-Sakakibara K,Provart NJ,Sakurai T,Shimada Y,Saito K

更新日期：2010-02-01 00:00:00

abstract：:Plants accumulate the lipids phosphatidic acid (PA), diacylglycerol (DAG), and triacylglycerol (TAG) during cold stress, but how plants balance the levels of these lipids to mediate cold responses remains unknown. The enzymes ACYL-COENZYME A:DIACYLGLYCEROL ACYLTRANSFERASE (DGAT) and DIACYLGLYCEROL KINASE (DGK) catalyz...

journal_title：Plant physiology

authors： Tan WJ,Yang YC,Zhou Y,Huang LP,Xu L,Chen QF,Yu LJ,Xiao S

更新日期：2018-07-01 00:00:00

abstract：:Although cytokinins (CKs) affect a number of processes connected with chloroplasts, it has never been rigorously proven that chloroplasts contain CKs. We isolated intact chloroplasts from tobacco (Nicotiana tabacum L. cv SR1) and wheat (Triticum aestivum L. cv Ritmo) leaves and determined their CKs by liquid chromatog...

journal_title：Plant physiology

authors： Benková E,Witters E,Van Dongen W,Kolár J,Motyka V,Brzobohatý B,Van Onckelen HA,Machácková I

更新日期：1999-09-01 00:00:00

abstract：:The effects of nitrogen source NO(3) (-) or NH(4) (+) on nitrogen metabolism during the first 2 weeks of germination of the rice seedling (Oryza sativa L., var. IR22) grown in nutrient solution containing 40 mug/ml N were studied. Total, soluble protein, and free amino N levels were higher in the NH(4) (+)-grown seedl...

journal_title：Plant physiology

authors： Marwaha RS,Juliano BO

更新日期：1976-06-01 00:00:00

abstract：:A radioimmunoassay for glyceollin I, the major phytoalexin produced by soybean (Glycine max [L.] Merr.), has been developed. Antibodies were raised in rabbits against a glyceollin I-bovine serum albumin conjugate. The antisera were used to establish a radioimmunoassay for glyceollin I using [(125)I]glyceollin I as the...

journal_title：Plant physiology

authors： Moesta P,Hahn MG,Grisebach H

更新日期：1983-10-01 00:00:00

abstract：:Diatoms constitute a major phylum of phytoplankton biodiversity in ocean water and freshwater ecosystems. They are known to respond to some chemical variations of the environment by the accumulation of triacylglycerol, but the relative changes occurring in membrane glycerolipids have not yet been studied. Our goal was...

journal_title：Plant physiology

authors： Abida H,Dolch LJ,Meï C,Villanova V,Conte M,Block MA,Finazzi G,Bastien O,Tirichine L,Bowler C,Rébeillé F,Petroutsos D,Jouhet J,Maréchal E

更新日期：2015-01-01 00:00:00

abstract：:Constitutive arginase (molecular weight 330,000) 920-fold purified from Evernia prunastri thallus, is activated by putrescine, l-ornithine, and agmatine with K(a) values of 2.7, 1.1, and 5.8 millimolar, respectively. Constitutive arginase is also activated by endogenous l-arginine, reaching its maximum activity at 16 ...

journal_title：Plant physiology

authors： Martín-Falquina A,Legaz ME

更新日期：1984-12-01 00:00:00

abstract：:Peptide substrates of well-defined protein kinases were microinjected into aleurone protoplasts of barley (Hordeum vulgare L. cv Himalaya) to inhibit, and therefore identify, protein kinase-regulated events in the transduction of the gibberellin (GA) and abscisic acid signals. Syntide-2, a substrate designed for Ca2+-...

journal_title：Plant physiology

authors： Ritchie S,Gilroy S

更新日期：1998-02-01 00:00:00

abstract：:Understanding the biochemistry of triacylglycerol (TAG) assembly is critical in tailoring seed oils to produce high-value products. Hydroxy-fatty acid (HFA) is one such valuable modified fatty acid, which can be produced at low levels in Arabidopsis (Arabidopsis thaliana) seed through transgenic expression of the cast...

journal_title：Plant physiology

authors： Lunn D,Wallis JG,Browse J

更新日期：2019-03-01 00:00:00

abstract：:Evidence that indoleacetic acid (IAA) conjugates are metabolized via enzyme-catalyzed hydrolysis to free IAA and that their biological activities are related to the rates at which they are hydrolyzed by the tissue is presented. These conclusions are based on the following observations. Slow but continuous decarboxylat...

journal_title：Plant physiology

authors： Hangarter RP,Good NE

更新日期：1981-12-01 00:00:00

abstract：:The sensitivity of lettuce (Lactuca sativa L. cv Grand Rapids) seeds to red light was reduced by NaCl concentrations which had no effect upon the germination of continuously illuminated seeds. The germination capacity of the seeds was fully restored by increased red light exposures. Indirect evidence indicates that Na...

journal_title：Plant physiology

authors： Scorer KN,Epel BL,Waisel Y

更新日期：1985-09-01 00:00:00

abstract：:Plants of the wilty tomato (Lycopersicum esculentum) mutant, flacca, and of the normal cultivar Rheinlands Ruhm growing under either "normal" or high humidity were used in this research. Under normal humidity, RNase activity was much higher in mutant plants in which abscisic acid (ABA) and water content were lower tha...

journal_title：Plant physiology

authors： Puri J,Tal M

更新日期：1977-02-01 00:00:00

abstract：:The in vitro degradation of green tomato (Lycopersicon esculentum L.) cell walls by an extract of ripe fruits was characterized. The susceptibility of isolated walls to enzymolysis varied considerably among the different cultivars tested. Wall solubilization in vitro appeared to be nearly as extensive as that which ac...

journal_title：Plant physiology

authors： Wallner SJ,Bloom HL

更新日期：1977-08-01 00:00:00

abstract：:The effect of strong irradiance (2000 micromole photons per square meter per second) on PSII heterogeneity in intact cells of Chlamydomonas reinhardtii was investigated. Low light (LL, 15 micromole photons per square meter per second) grown C. reinhardtii are photoinhibited upon exposure to strong irradiance, and the ...

journal_title：Plant physiology

authors： Neale PJ,Melis A

更新日期：1990-04-01 00:00:00

abstract：:Chloroplast NADPH-thioredoxin reductase (NTRC) belongs to the thioredoxin systems that control crucial metabolic and regulatory pathways in plants. Here, by characterization of T-DNA insertion lines of NTRC gene, we uncover a novel connection between chloroplast thiol redox regulation and the control of photoperiodic ...

journal_title：Plant physiology

authors： Lepistö A,Kangasjärvi S,Luomala EM,Brader G,Sipari N,Keränen M,Keinänen M,Rintamäki E

更新日期：2009-03-01 00:00:00

abstract：:When water uptake by growing cells is prevented, the turgor pressure and the tensile stress in the cell wall are reduced by continued wall loosening. This process, termed in vivo stress relaxation, provides a new way to study the dynamics of wall loosening and to measure the wall yield threshold and the physiological...

journal_title：Plant physiology

authors： Cosgrove DJ

更新日期：1987-01-01 00:00:00

abstract：:The ontogeny of seed structure and the accumulation of seed storage substances is the result of a determinant genetic program. Using RNA interference, the synthesis of soybean (Glycine max) glycinin and conglycinin storage proteins has been suppressed. The storage protein knockdown (SP-) seeds are overtly identical to...

journal_title：Plant physiology

authors： Schmidt MA,Barbazuk WB,Sandford M,May G,Song Z,Zhou W,Nikolau BJ,Herman EM

更新日期：2011-05-01 00:00:00

abstract：:We understand few details about how the arrangement and interactions of cell wall polymers produce the mechanical properties of primary cell walls. Consequently, we cannot quantitatively assess if proposed wall structures are mechanically reasonable or assess the effectiveness of proposed mechanisms to change mechanic...

journal_title：Plant physiology

authors： Kha H,Tuble SC,Kalyanasundaram S,Williamson RE

更新日期：2010-02-01 00:00:00

abstract：:Histone acetylation is an important posttranslational modification correlated with gene activation. In Arabidopsis (Arabidopsis thaliana), the histone acetyltransferase AtHAC1 is homologous to animal p300/CREB (cAMP-responsive element-binding protein)-binding proteins, which are the main histone acetyltransferases par...

journal_title：Plant physiology

authors： Deng W,Liu C,Pei Y,Deng X,Niu L,Cao X

更新日期：2007-04-01 00:00:00

abstract：:After the application of 2.36 Curies per millimole [2,3-(3)H]gibberellin A(20) (GA(20)) to 21-day-old maize (Zea mays L., hybrid CM7 x CM49) plants, etiolated maize seedlings, or maturing maize cobs, a number of (3)H-metabolites were observed. The principal acidic (pH 3.0), ethyl acetate-soluble metabolite was identif...

journal_title：Plant physiology

authors： Rood SB,Koshioka M,Douglas TJ,Pharis RP

更新日期：1982-12-01 00:00:00

abstract：:The present report shows that the effect of red and far red light on the synthesis of phenylalanine ammonia-lyase can be ascribed to the action of phytochrome Pfr. This is true not only for short term irradiation but also for the action of continuous far red light. In the latter case, the model worked out by Hartmann ...

journal_title：Plant physiology

authors： Schopfer P,Mohr H

更新日期：1972-01-01 00:00:00

abstract：:Some clones of Spirodela polyrrhiza form dormant bodies called turions which require several weeks of chilling treatment before they proceed to renew growth and develop into vegetative fronds. The individual fronds of Spirodela are less than 5 mm long and can be grown aseptically in liquid culture. Turion formation an...

journal_title：Plant physiology

authors： Perry TO

更新日期：1968-11-01 00:00:00

abstract：:Formation of the riboside-5'-monophosphate is a general feature of the metabolism of cytokinins in plants. As part of a study of the biological significance of the nucleotide form of cytokinins, we analyzed a mutant of Arabidopsis thaliana deficient in adenine phosphoribosyltransferase (APRT) activity for its ability ...

journal_title：Plant physiology

authors： Moffatt B,Pethe C,Laloue M

更新日期：1991-03-01 00:00:00

abstract：:To define the functions of genes previously identified by expression profiling as being rapidly light induced under phytochrome (phy) control, we are investigating the seedling de-etiolation phenotypes of mutants carrying T-DNA insertional disruptions at these loci. Mutants at one such locus displayed reduced responsi...

journal_title：Plant physiology

authors： Khanna R,Kikis EA,Quail PH

更新日期：2003-12-01 00:00:00