Cyclin-dependent kinase inhibitors in maize endosperm and their potential role in endoreduplication.

Abstract:

:Two maize (Zea mays) cyclin-dependent kinase (CDK) inhibitors, Zeama;KRP;1 and Zeama;KRP;2, were characterized and shown to be expressed in developing endosperm. Similar to the CDK inhibitors in Arabidopsis (Arabidopsis thaliana) and tobacco (Nicotiana tabacum), the maize proteins contain a carboxy-terminal region related to the inhibitory domain of the mammalian Cip/Kip inhibitors. Zeama;KRP;1 is present in the endosperm between 7 and 21 d after pollination, a period that encompasses the onset of endoreduplication, while the Zeama;KRP;2 protein declines during this time. Nevertheless, Zeama;KRP;1 accounts for only part of the CDK inhibitory activity that peaks coincident with the endoreduplication phase of endosperm development. In vitro assays showed that Zeama;KRP;1 and Zeama;KRP;2 are able to inhibit endosperm Cdc2-related CKD activity that associates with p13(Suc1). They were also shown to specifically inhibit cyclin A1;3- and cyclin D5;1-associated CDK activities, but not cyclin B1;3/CDK. Overexpression of Zeama;KRP;1 in maize embryonic calli that ectopically expressed the wheat dwarf virus RepA protein, which counteracts retinoblastoma-related protein function, led to an additional round of DNA replication without nuclear division.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Coelho CM,Dante RA,Sabelli PA,Sun Y,Dilkes BP,Gordon-Kamm WJ,Larkins BA

doi

10.1104/pp.105.063917

subject

Has Abstract

pub_date

2005-08-01 00:00:00

pages

2323-36

issue

4

eissn

0032-0889

issn

1532-2548

pii

pp.105.063917

journal_volume

138

pub_type

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