Abstract:
:The Golgi apparatus consists of stacked cisternae filled with enzymes that facilitate the sequential and highly controlled modification of glycans from proteins that transit through the organelle. Although the glycan processing pathways have been extensively studied, the underlying mechanisms that concentrate Golgi-resident glycosyltransferases and glycosidases in distinct Golgi compartments are poorly understood. The single-pass transmembrane domain (TMD) of n-acetylglucosaminyltransferaseI (GnTI) accounts for its steady-state distribution in the cis/medial-Golgi. Here, we investigated the contribution of individual amino acid residues within the TMD of Arabidopsis (Arabidopsis thaliana) and Nicotiana tabacum GnTI toward Golgi localization and n-glycan processing. Conserved sequence motifs within the TMD were replaced with those from the established trans-Golgi enzyme α2,6-sialyltransferase and site-directed mutagenesis was used to exchange individual amino acid residues. Subsequent subcellular localization of fluorescent fusion proteins and n-glycan profiling revealed that a conserved Gln residue in the GnTI TMD is essential for its cis/medial-Golgi localization. Substitution of the crucial Gln residue with other amino acids resulted in mislocalization to the vacuole and impaired n-glycan processing in vivo. Our results suggest that sequence-specific features of the GnTI TMD are required for its interaction with a Golgi-resident adaptor protein or a specific lipid environment that likely promotes coat protein complexI-mediated retrograde transport, thus maintaining the steady-state distribution of GnTI in the cis/medial-Golgi of plants.
journal_name
Plant Physioljournal_title
Plant physiologyauthors
Schoberer J,Liebminger E,Vavra U,Veit C,Grünwald-Gruber C,Altmann F,Botchway SW,Strasser Rdoi
10.1104/pp.19.00310subject
Has Abstractpub_date
2019-06-01 00:00:00pages
859-873issue
2eissn
0032-0889issn
1532-2548pii
pp.19.00310journal_volume
180pub_type
杂志文章相关文献
PLANT PHYSIOLOGY文献大全abstract::Previous work (D.P. Delmer, J. Pear, A. Andrawis, D. Stalker [1995] Mol Gen Genet 248: 43-51) has identified a gene in cotton (Gossypium hirsutum), Rac13, that encodes a small, signal-transducing GTPase and shows high expression in the fiber at the time of transition from primary to secondary wall synthesis. Since Rac...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.112.4.1491
更新日期:1996-12-01 00:00:00
abstract::The evolutionarily conserved YidC/Oxa1/Alb3 proteins are involved in the insertion of membrane proteins in all domains of life. In plant mitochondria, individual knockouts of OXA1a, OXA2a, and OXA2b are embryo-lethal. In contrast to other members of the protein family, OXA2a and OXA2b contain a tetratricopeptide repea...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.18.01286
更新日期:2019-02-01 00:00:00
abstract::Germination potential in nondormant, upper cocklebur (Xanthium pensylvanicum Wallr.) seeds, which were incapable of germinating under constant temperatures below 25 C in air, was increased by exposure to diurnally alternating temperatures. The cocklebur seeds failed to respond to the temperature fluctuations in the be...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.61.3.437
更新日期:1978-03-01 00:00:00
abstract::An arginase, purified from the leaf of the jack bean, Canavalia ensiformis, can effectively hydrolyze both l- and d-arginine. Arginases, examined from a number of other plant and animal sources, exhibit marked substrate stereospecificity and fail to catabolize d-arginine. In order to provide essential nitrogen, jack b...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.94.1.67
更新日期:1990-09-01 00:00:00
abstract::The separate package and spillover models of photosynthetic enhancement are generalized and extended to light saturation assuming hyperbolic or exponential rate versus intensity curves. The available data are more consistent with the exponential model at high light intensities. Two significant conclusions follow: (a) ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.47.2.222
更新日期:1971-02-01 00:00:00
abstract::Chilling at 4 degrees C in the dark induced lipid degradation in cucumber (Cucumis sativa L.) fruit upon rewarming at 14 degrees C. Rates of ethane evolution by fruits rewarmed after 3 days of chilling were up to four-fold higher than those evolved by unchilled (14 degrees C) fruits (0.02-0.05 picomoles gram fresh wei...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.90.3.1049
更新日期:1989-07-01 00:00:00
abstract::Centromeres are required for faithful segregation of chromosomes in cell division. It is not clear what kind of sequences act as functional centromeres and how centromere sequences are organized in Oryza punctata, a BB genome species. In this study, we found that the CentO centromeric satellites in O. punctata share h...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.105.064147
更新日期:2005-09-01 00:00:00
abstract::Ion and oxygen uptake were studied on aging bean stem slices. Oxygen uptake was high immediately after slicing, decreased to a minimum at 100 minutes, and then increased again. Ion uptake per unit of O(2) uptake data suggested that metabolic energy was utilized almost exclusively for sodium transport in fresh tissue b...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.47.5.663
更新日期:1971-05-01 00:00:00
abstract::Although air-filled intercellular spaces are necessary and ubiquitous in higher plants, little attention has been paid to the possible mechanisms by which these spaces are kept from being flooded. The most likely mechanism is that the living plant cell may maintain a hydrophobic monolayer on the surfaces of adjacent i...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.72.4.989
更新日期:1983-08-01 00:00:00
abstract::Microsomal membranes from suspension-cultured Catharanthus roseus cells possess an enzymic activity that catalyzes the ATP-dependent phosphorylation of phosphatidic acid (PA) to form diacylglycerol pyrophosphate (H. Behrbohm, J.B. Wissing [1993] FEBS Lett 315: 95-99). This enzyme activity, PA kinase, was purified and ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.102.4.1243
更新日期:1993-08-01 00:00:00
abstract::We have used (13)C-labeled sugars and nuclear magnetic resonance (NMR) spectrometry to study the metabolic pathway of starch biosynthesis in developing wheat grain (Triticum aestivum cv Mardler). Our aim was to examine the extent of redistribution of (13)C between carbons atoms 1 and 6 of [1-(13)C] or [6-(13)C]glucose...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.87.2.311
更新日期:1988-06-01 00:00:00
abstract::At least two hydroxypyruvate reductases (HPRs), differing in specificity for NAD(P)H and (presumably) utilizing glyoxylate as a secondary substrate, were identified by fractionation of crude maize leaf extracts with ammonium sulfate. The NADH-preferring enzyme, which most probably represented peroxisomal HPR, was prec...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.91.1.278
更新日期:1989-09-01 00:00:00
abstract::Assessing the genetic variability of plant performance under heat and drought scenarios can contribute to reduce the negative effects of climate change. We propose here an approach that consisted of (1) clustering time courses of environmental variables simulated by a crop model in current (35 years × 55 sites) and fu...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.16.00621
更新日期:2016-10-01 00:00:00
abstract::The fhy3 mutation of Arabidopsis impairs phytochrome A (phyA)-mediated inhibition of hypocotyl growth without affecting the levels of phyA measured spectrophotometrically or immunochemically. We investigated whether the fhy3-1 mutation has similar effects on very low fluence responses (VLFR) and high irradiance respon...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.123.1.235
更新日期:2000-05-01 00:00:00
abstract::The effect of the exogenous and endogenous NO(3) (-) concentration on net uptake, influx, and efflux of NO(3) (-) and on nitrate reductase activity (NRA) in roots was studied in Phaseolus vulgaris L. cv. Witte Krombek. After exposure to NO(3) (-), an apparent induction period of about 6 hours occurred regardless of th...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.70.3.754
更新日期:1982-09-01 00:00:00
abstract::Levels of the enzymes that produce wound response mediators have to be controlled tightly in unwounded tissues. The Arabidopsis (Arabidopsis thaliana) fatty acid oxygenation up-regulated8 (fou8) mutant catalyzes high rates of alpha -linolenic acid oxygenation and has higher than wild-type levels of the alpha -linoleni...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.109.150474
更新日期:2010-03-01 00:00:00
abstract::Rhythmic fluctuation in the basipetal movement of auxin occurs in corn (Zea mays) coleoptiles oriented either in the vertical or in the horizontal position. This periodicity of transport rate varies from region to region in a horizontal coleoptile. Between an upper and lower half coleoptile (with respect to gravity), ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.52.2.166
更新日期:1973-08-01 00:00:00
abstract::Cytokinin proved to be a controlling factor in sieve tube regeneration around wounded collateral bundles in an in vivo system in which the endogenous cytokinin level had been minimized. Both kinetin and zeatin were applied in aqueous solution to the bases of excised, mature internodes of Coleus blumei Benth. that had ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.93.3.982
更新日期:1990-07-01 00:00:00
abstract::Carefully curated proteomes of the inner envelope membrane, the thylakoid membrane, and the thylakoid lumen of chloroplasts from Arabidopsis were assembled based on published, well-documented localizations. These curated proteomes were evaluated for distribution of physical-chemical parameters, with the goal of extrac...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.104.040717
更新日期:2004-06-01 00:00:00
abstract::The Mg-nucleoside triphosphatase activity associated with the inner envelope membrane of the pea chloroplast is comprised of at least two components, a major activity that is sensitive to vanadate and sodium fluoride and a minor insensitive activity. The vanadate/fluoride sensitive activity has been partially purified...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.80.4.908
更新日期:1986-04-01 00:00:00
abstract::Vacuolar sorting receptors, BP80/VSRs, play a critical role in vacuolar trafficking of soluble proteins in plant cells. However, the mechanism of action of BP80 is not well understood. Here, we investigate the action mechanism of AtVSR1, a member of BP80 proteins in Arabidopsis (Arabidopsis thaliana), in vacuolar traf...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.110.159814
更新日期:2010-09-01 00:00:00
abstract::The effects of the diphenyl ether herbicides HOE 29152 (methyl-2[4-(4-trifluoromethoxy) phenoxy] propanoate) and nitrofluorfen (2-chloro-1-[4-nitrophenoxy]-4-[trifluoromethyl]benzene) on photosynthetic electron transport have been examined with pea seedling and spinach chloroplasts. Linear electron transport (water to...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.65.1.47
更新日期:1980-01-01 00:00:00
abstract::The JASMONATE ZIM DOMAIN (JAZ) proteins function as negative regulators of jasmonic acid signaling in plants. We cloned 12 JAZ genes from native tobacco (Nicotiana attenuata), including nine novel JAZs in tobacco, and examined their expression in plants that had leaves elicited by wounding or simulated herbivory. Most...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.112.193771
更新日期:2012-06-01 00:00:00
abstract::Flowering of the facultative long-day plant Arabidopsis is controlled by several endogenous and environmental factors, among them gibberellins (GAs) and day length. The promotion of flowering by long days involves an endogenous clock that interacts with light cues provided by the environment. Light, and specifically p...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.007625
更新日期:2002-12-01 00:00:00
abstract::Three unique classes of carbohydrates were isolated from the hyphal cell walls of Phytophthora megasperma f.sp. glycinea (Pmg) and compared with other substances for their activity as elicitors of the phytoalexin glyceollin in soybean tissues. Glucomannans extracted from cell walls with soybean beta-1,3-endoglucanase ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.71.3.466
更新日期:1983-03-01 00:00:00
abstract::In this study the interplay of mitochondria and peroxisomes in photorespiration was simulated in a reconstituted system of isolated mitochondria and peroxisomes from spinach (Spinacia oleracea L.) leaves. The mitochondria oxidizing glycine produced serine, which was reduced in the peroxisomes to glycerate. The require...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.116.4.1333
更新日期:1998-04-01 00:00:00
abstract::We report here a method for the isolation of high specific activity phosphoglycerate kinase (EC 2.7.2.3) from chloroplasts. The enzyme has been purified over 200-fold from pea (Pisum sativum L.) stromal extracts to apparent homogeneity with 23% recovery. Negative cooperativity is observed with the two enzyme phosphogl...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.94.1.291
更新日期:1990-09-01 00:00:00
abstract::Cell-free extracts of Chlorella pyrenoidosa contained two enzymes capable of oxidizing d-lactate; these were glycolate dehydrogenase and NAD(+)-dependent d-lactate dehydrogenase. The two enzymes could be distinguished by differential centrifugation, glycolate dehydrogenase being largely particulate and NAD(+)-d-lactat...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.53.2.167
更新日期:1974-02-01 00:00:00
abstract::The in vivo phosphorylation of starch was studied in Solanum tuberosum cv Dianella and Posmo. Small starch granules contain 25% more ester-bound phosphate per glucose residue than large starch granules. The degree of phosphorylation was found to be almost constant during tuber development. Isolated tuber discs synthes...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.105.1.111
更新日期:1994-05-01 00:00:00
abstract::Eukaryotic Δ6-desaturases are microsomal enzymes that balance the synthesis of ω-3 and ω-6 C18-polyunsaturated fatty acids (C18-PUFAs) according to their specificity. In several microalgae, including Ostreococcus tauri, plastidic C18-PUFAs are strictly regulated by environmental cues suggesting an autonomous control o...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.20.00281
更新日期:2020-09-01 00:00:00