Isolation of chloroplastic phosphoglycerate kinase : kinetics of the two-enzyme phosphoglycerate kinase/glyceraldehyde-3-phosphate dehydrogenase couple.

abstract：:An ATPase preparation, presumed to be associated with plasma membrane due to the coincidence on isopycnic gradients of cellulase and beta-glucan synthetase at high substrate, has been isolated from the epidermal and mesophyll of tobacco leaf. The ATPase from both tissues was found to prefer ATP over other nucleotides....

journal_title：Plant physiology

authors： Lurie S

更新日期：1979-05-01 00:00:00

abstract：:A mechanism that confers increased Al resistance in the Arabidopsis thaliana mutant alr-104 was investigated. A modified vibrating microelectrode system was used to measure H+ fluxes generated along the surface of small Arabidopsis roots. In the absence of Al, no differences in root H+ fluxes between wild type and alr...

journal_title：Plant physiology

authors： Degenhardt J,Larsen PB,Howell SH,Kochian LV

更新日期：1998-05-01 00:00:00

abstract：:Photosynthetic activity and organization of chlorophyll(Chl)-protein complexes in a temperature sensitive mutant of Chlorella pyrenoidosa have been investigated. The mutant is practically indistinguishable from wild type cells when grown at 25 C. However, mutant cells grown at 33 C do not synthesize Chl and lose their...

journal_title：Plant physiology

authors： Lavintman N,Galling G,Ohad I

更新日期：1981-12-01 00:00:00

abstract：:A synthetic green fluorescent protein (GFP) gene (pgfp) was constructed to improve GFP expression in plants. Corn and tobacco protoplast transient assays showed that pgfp gave about 20-fold brighter fluorescence than the wild-type gene (gfp). Replacement of the serine at position 65 with a threonine (S65Tpgfp) or a cy...

journal_title：Plant physiology

authors： Pang SZ,DeBoer DL,Wan Y,Ye G,Layton JG,Neher MK,Armstrong CL,Fry JE,Hinchee MA,Fromm ME

更新日期：1996-11-01 00:00:00

abstract：:The starch-granule-bound starch synthase from Zea mays has been solubilized with a recovery of between 50 and 84%. Chromatography of the solubilized enzyme on DEAE-Sepharose resolves two fractions of activity which may be distinguished by their response to citrate. Neither solubilized isoenzyme displays any significan...

journal_title：Plant physiology

authors： Macdonald FD,Preiss J

更新日期：1983-09-01 00:00:00

abstract：:In higher plants, the most abundant sterol derivatives are steryl glycosides (SGs) and acyl SGs. Arabidopsis (Arabidopsis thaliana) contains two genes, UGT80A2 and UGT80B1, that encode UDP-Glc:sterol glycosyltransferases, enzymes that catalyze the synthesis of SGs. Lines having mutations in UGT80A2, UGT80B1, or both U...

journal_title：Plant physiology

authors： DeBolt S,Scheible WR,Schrick K,Auer M,Beisson F,Bischoff V,Bouvier-Navé P,Carroll A,Hematy K,Li Y,Milne J,Nair M,Schaller H,Zemla M,Somerville C

更新日期：2009-09-01 00:00:00

abstract：:We have constructed a chimeric gene consisting of the promoter, first exon, and first intron of a maize ubiquitin gene (Ubi-1) and the coding sequence of the bar gene from Streptomyces hygroscopicus. This construct was transferred into rice (Oryza sativa L.) protoplasts via electroporation, and 10 plants were regenera...

journal_title：Plant physiology

authors： Toki S,Takamatsu S,Nojiri C,Ooba S,Anzai H,Iwata M,Christensen AH,Quail PH,Uchimiya H

更新日期：1992-11-01 00:00:00

abstract：:The spontaneous occurrence of resistance to the herbicide glyphosate in weed species has been an extremely infrequent event, despite over 20 years of extensive use. Recently, a glyphosate-resistant biotype of goosegrass (Eleusine indica) was identified in Malaysia exhibiting an LD(50) value approximately 2- to 4-fold ...

journal_title：Plant physiology

authors： Baerson SR,Rodriguez DJ,Tran M,Feng Y,Biest NA,Dill GM

更新日期：2002-07-01 00:00:00

abstract：:To examine the role of isoamylase1 (ISA1) in amylopectin biosynthesis in plants, a genomic DNA fragment from Aegilops tauschii was introduced into the ISA1-deficient rice (Oryza sativa) sugary-1 mutant line EM914, in which endosperm starch is completely replaced by phytoglycogen. A. tauschii is the D genome donor of w...

journal_title：Plant physiology

authors： Kubo A,Rahman S,Utsumi Y,Li Z,Mukai Y,Yamamoto M,Ugaki M,Harada K,Satoh H,Konik-Rose C,Morell M,Nakamura Y

更新日期：2005-01-01 00:00:00

abstract：:Freezing, dehydration, and supercooling cause microtubules in mesophyll cells of spinach (Spinacia oleracea L. cv Bloomsdale) to depolymerize (ME Bartolo, JV Carter [1991] Plant Physiol 97: 175-181). The objective of this study was to gain insight into the question of whether microtubules depolymerize as a direct resp...

journal_title：Plant physiology

authors： Bartolo ME,Carter JV

更新日期：1992-08-01 00:00:00

abstract：:Mannitol-1-phosphate (M1P) dehydrogenase (M1PDH; EC 1.1.1.17), an enzyme catalyzing the reduction of Fru-6-phosphate (F6P) to M1P in algal mannitol biosynthesis, was purified to homogeneity from a cell homogenate of the eulittoral red alga Caloglossa continua (Okamura) King et Puttock. The enzyme was a monomer with an...

journal_title：Plant physiology

authors： Iwamoto K,Kawanobe H,Ikawa T,Shiraiwa Y

更新日期：2003-10-01 00:00:00

abstract：:Chlorophyll a fluorescence of Synechococcus UTEX 625 was quenched during the transport of inorganic carbon, even when CO(2) fixation was inhibited by iodoacetamide. Measurements with a pulse modulation fluorometer showed that at least 75% of the quenching was due to oxidation of Q(a), the primary acceptor of photosyst...

journal_title：Plant physiology

authors： Miller AG,Espie GS,Canvin DT

更新日期：1988-09-01 00:00:00

abstract：:The major light-harvesting chlorophyll a/b binding protein (LHCP) of higher plant chloroplasts is nuclear-encoded, synthesized as a precursor, and processed upon import. We have previously (GK Lamppa, M Abad [1987] J Cell Biol 105: 2641-2648) identified a soluble enzyme that cleaves the LHCP precursor (pLHCP). In this...

journal_title：Plant physiology

authors： Abad MS,Clark SE,Lamppa GK

更新日期：1989-05-01 00:00:00

abstract：:Photorespiration rates in tissue cultures of a C(4) plant, Portulaca oleracea, were compared to those in tissue cultures of a C(3) plant, Streptanthus tortuosus. The C(4) plant tissue cultures have one-half to one-third the photorespiration rate of the C(3) plant tissue cultures and respond to varying O(2) concentrati...

journal_title：Plant physiology

authors： Kennedy RA

更新日期：1976-10-01 00:00:00

abstract：:Tonoplast-enriched membranes were prepared from maize (Zea mays L. cv LG 11) primary roots, using sucrose nonlinear gradients. The functional molecular size of the tonoplast ATP-and PPi-dependent proton pumps were analyzed by radiation inactivation. Glucose-6-phosphate dehydrogenase (G6PDH) was added as an internal st...

journal_title：Plant physiology

authors： Chanson A,Pilet PE

更新日期：1989-07-01 00:00:00

abstract：:The influence of plant spacing and row orientation on spectral distribution of light received by growing soybean (Gylcine max [L.] Merr.) plants was measured under field conditions. Light absorption, reflection and transmission of individual leaves showed that most of the blue and red was absorbed while most of the fa...

journal_title：Plant physiology

authors： Kasperbauer MJ

更新日期：1987-10-01 00:00:00

abstract：:Sucrose synthase (SUS) is phosphorylated on a major, amino-terminal site located at Ser-15 (S15) in the maize (Zea mays) SUS1 protein. Site- and phospho-specific antibodies against a phosphorylated S15 (pS15) peptide allowed direct analysis of S15 phosphorylation in relation to membrane association. Immunoblots of the...

journal_title：Plant physiology

authors： Hardin SC,Winter H,Huber SC

更新日期：2004-04-01 00:00:00

abstract：:We have isolated the plasma membrane H+-ATPase in a phosphorylated form from spinach (Spinacia oleracea L.) leaf tissue incubated with fusicoccin, a fungal toxin that induces irreversible binding of 14-3-3 protein to the C terminus of the H+-ATPase, thus activating H+ pumping. We have identified threonine-948, the sec...

journal_title：Plant physiology

authors： Olsson A,Svennelid F,Ek B,Sommarin M,Larsson C

更新日期：1998-10-01 00:00:00

abstract：:As part of a project to investigate the mechanism of cortical microtubule (MT) alignment, we examined the effects of cycloheximide (CHM) on cortical MTs in the root tip cells of Allium cepa L. Results show that although a preprophase band of MTs remained in the cell cortex, interphase MTs disappeared from the cortical...

journal_title：Plant physiology

authors： Mineyuki Y,Iida H,Anraku Y

更新日期：1994-01-01 00:00:00

abstract：:The promoter of phytoene synthase, the first specific enzyme of carotenoid biosynthesis, shows two main regulatory regions: a G-box-containing region located near the TATA box, and a TATA box distal region containing the cis-acting element ATCTA, which mediates strong basal promoter activity. This second element was a...

journal_title：Plant physiology

authors： Welsch R,Maass D,Voegel T,Dellapenna D,Beyer P

更新日期：2007-11-01 00:00:00

abstract：:Plant defense against pathogens depends on the action of several endogenously produced hormones, including jasmonic acid (JA) and ethylene. In certain defense responses, JA and ethylene signaling pathways synergize to activate a specific set of defense genes. Here, we describe the role of the Arabidopsis (Arabidopsis ...

journal_title：Plant physiology

authors： Pré M,Atallah M,Champion A,De Vos M,Pieterse CM,Memelink J

更新日期：2008-07-01 00:00:00

abstract：:The water-soluble polysaccharides (SEPS) secreted into the medium by suspension-cultured sycamore cells were examined to determine whether the polysaccharides were the same as those present in the walls of sycamore cells. The SEPS were made more amenable to fractionation by treatment with a highly purified alpha-1,4-e...

journal_title：Plant physiology

authors： Stevenson TT,McNeil M,Darvill AG,Albersheim P

更新日期：1986-04-01 00:00:00

abstract：:Using (14)CO(2) gas exchange and metabolite analyses, stomatal as well as total internal CO(2) uptake and evolution were estimated. Pulse modulated fluorescence was measured during induction and steady state of photosynthesis. Leaf water potential of Digitalis lanata EHRH. plants decreased to -2.5 megapascals after wi...

journal_title：Plant physiology

authors： Stuhlfauth T,Scheuermann R,Fock HP

更新日期：1990-04-01 00:00:00

abstract：:Increased ethylene synthesis was rapidly induced throughout the apical meristematic region of etiolated seedlings of Pisum sativum L., cv. Alaska by cuts made 1 centimeter from the apical hook. The wound signal was transmitted at about 2 millimeters per minute. Accumulation of substance(s) at the cut surfaces of excis...

journal_title：Plant physiology

authors： Saltveit ME,Dilley DR

更新日期：1978-11-01 00:00:00

abstract：:We examined the influence of aluminum and calcium (and certain other cations) on hormone transport in corn roots. When aluminum was applied unilaterally to the caps of 15 mm apical root sections the roots curved strongly away from the aluminum. When aluminum was applied unilaterally to the cap and 3H-indole-3-acetic...

journal_title：Plant physiology

authors： Hasenstein KH,Evans ML

更新日期：1988-01-01 00:00:00

abstract：:Cytokinin proved to be a controlling factor in sieve tube regeneration around wounded collateral bundles in an in vivo system in which the endogenous cytokinin level had been minimized. Both kinetin and zeatin were applied in aqueous solution to the bases of excised, mature internodes of Coleus blumei Benth. that had ...

journal_title：Plant physiology

authors： Aloni R,Baum SF,Peterson CA

更新日期：1990-07-01 00:00:00

abstract：:To study the effect of O(2) on the photosynthetic and glycolate pathways, maize leaves were exposed to (14)CO(2) during steady-state photosynthesis in 21 or 1% O(2). At the two O(2) concentrations after a (14)CO(2) pulse (4 seconds) followed by a (12)CO(2) chase, there was a slight difference in CO(2) uptake and in th...

journal_title：Plant physiology

authors： Morot-Gaudry JF

更新日期：1980-12-01 00:00:00

abstract：:Understanding how plants sense and respond to changes in nitrogen availability is the first step toward developing strategies for biotechnological applications, such as improvement of nitrogen use efficiency. However, components involved in nitrogen signaling pathways remain poorly characterized. Calcium is a second m...

journal_title：Plant physiology

authors： Riveras E,Alvarez JM,Vidal EA,Oses C,Vega A,Gutiérrez RA

更新日期：2015-10-01 00:00:00

abstract：:The location and changes in NAD(P)H have been monitored during oscillatory growth in pollen tubes of lily (Lilium formosanum) using the endogenous fluorescence of the reduced coenzyme (excitation, 360 nm; emission, >400 nm). The strongest signal resides 20 to 40 microm behind the apex where mitochondria (stained with ...

journal_title：Plant physiology

authors： Cárdenas L,McKenna ST,Kunkel JG,Hepler PK

更新日期：2006-12-01 00:00:00

abstract：:The dark respiration rate of discs from fully expanded tobacco leaves (Nicotiana tabacum) increased linearly with decreasing diameter, the relative increase being independent of leaf age. The wound respiration responsible for this situation reached a plateau within 15 minutes of excision. Metabolite analysis gave evid...

journal_title：Plant physiology

authors： Macnicol PK

更新日期：1976-01-01 00:00:00