Abstract:
:Understanding how plants sense and respond to changes in nitrogen availability is the first step toward developing strategies for biotechnological applications, such as improvement of nitrogen use efficiency. However, components involved in nitrogen signaling pathways remain poorly characterized. Calcium is a second messenger in signal transduction pathways in plants, and it has been indirectly implicated in nitrate responses. Using aequorin reporter plants, we show that nitrate treatments transiently increase cytoplasmic Ca(2+) concentration. We found that nitrate also induces cytoplasmic concentration of inositol 1,4,5-trisphosphate. Increases in inositol 1,4,5-trisphosphate and cytoplasmic Ca(2+) levels in response to nitrate treatments were blocked by U73122, a pharmacological inhibitor of phospholipase C, but not by the nonfunctional phospholipase C inhibitor analog U73343. In addition, increase in cytoplasmic Ca(2+) levels in response to nitrate treatments was abolished in mutants of the nitrate transceptor NITRATE TRANSPORTER1.1/Arabidopsis (Arabidopsis thaliana) NITRATE TRANSPORTER1 PEPTIDE TRANSPORTER FAMILY6.3. Gene expression of nitrate-responsive genes was severely affected by pretreatments with Ca(2+) channel blockers or phospholipase C inhibitors. These results indicate that Ca(2+) acts as a second messenger in the nitrate signaling pathway of Arabidopsis. Our results suggest a model where NRT1.1/AtNPF6.3 and a phospholipase C activity mediate the increase of Ca(2+) in response to nitrate required for changes in expression of prototypical nitrate-responsive genes.
journal_name
Plant Physioljournal_title
Plant physiologyauthors
Riveras E,Alvarez JM,Vidal EA,Oses C,Vega A,Gutiérrez RAdoi
10.1104/pp.15.00961subject
Has Abstractpub_date
2015-10-01 00:00:00pages
1397-404issue
2eissn
0032-0889issn
1532-2548pii
pp.15.00961journal_volume
169pub_type
杂志文章相关文献
PLANT PHYSIOLOGY文献大全abstract::Control of folate homeostasis is essential to sustain the demand for one-carbon (C1) units that are necessary for major biological functions, including nucleotide synthesis and methylation reactions. In this study, we analyzed the genome-wide and metabolic adaptive response of Arabidopsis (Arabidopsis thaliana) cells ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.108.130336
更新日期:2008-12-01 00:00:00
abstract::Chloroplast NADPH-thioredoxin reductase (NTRC) belongs to the thioredoxin systems that control crucial metabolic and regulatory pathways in plants. Here, by characterization of T-DNA insertion lines of NTRC gene, we uncover a novel connection between chloroplast thiol redox regulation and the control of photoperiodic ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.108.133777
更新日期:2009-03-01 00:00:00
abstract::Protein phosphorylation, catalyzed by the opposing actions of protein kinases and phosphatases, is a cornerstone of cellular signaling and regulation. Since their discovery, protein phosphatases have emerged as highly regulated enzymes with specificity that rivals their counteracting kinase partners. However, despite ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.111.182493
更新日期:2011-12-01 00:00:00
abstract::Following excision, etiolated epicotyl segments of Pisum sativum L. cv. Alaska exhibit a marked hyperpolarization of membrane potential which is followed by a linear accumulation of K(+) when segments are incubated in Higinbotham nutrient solution. Segments aged for several hours and then reexcised display only a slig...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.67.4.864
更新日期:1981-04-01 00:00:00
abstract::Since peroxidase and manganese have been implicated in both auxin destruction and ethylene production, the effect of auxins and high tissue levels of manganese on the peroxidative indoleacetic acid oxidase system and the internal level of ethylene was determined in cotton (Gossypium hirsutum L. cv. Watson GL-7). The h...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.49.4.555
更新日期:1972-04-01 00:00:00
abstract::The relative resistance of four cultivars of the Cucurbitaceae (Cucumis sativus L. cv. National Pickling, and inbred line SC 25; Cucurbita pepo L. cv. Prolific Straightneck Squash, and cv. Small Sugar Pumpkin) to SO(2) was determined. According to plots of the degree of exposure to SO(2) (which depends on the SO(2) co...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.61.5.761
更新日期:1978-05-01 00:00:00
abstract::Mitogen-activated protein kinase (MAPK) cascades play important roles in disease resistance in model plant species such as Arabidopsis (Arabidopsis thaliana) and tobacco (Nicotiana tabacum). However, the importance of MAPK signaling pathways in the disease resistance of crops is still largely uninvestigated. To better...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.111.185686
更新日期:2011-11-01 00:00:00
abstract::The accumulation and reduction of nitrate in the presence of the nitrogen metabolites asparagine (Asn) and glutamine (Gln) and the carbon metabolite sucrose (Suc) were examined in maize (Zea mays L.) seedlings in an attempt to separate their effects on the nitrate uptake system and the nitrate reduction system. After ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.114.2.583
更新日期:1997-06-01 00:00:00
abstract::A light-induced shrinkage of chloroplasts in vivo could be detected with chloroplasts isolated within 2 minutes of harvesting pea plants. As determined both by packed volume and Coulter counter, the mean volume of chloroplasts from plants in the dark was 39 mu(3), whereas it was 31 mu(3) for chloroplasts from plants i...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.43.5.781
更新日期:1968-05-01 00:00:00
abstract::Adenosine kinase (ADK) is a key enzyme that regulates intra- and extracellular levels of adenosine, thereby modulating methyltransferase reactions, production of polyamines and secondary compounds, and cell signaling in animals. Unfortunately, little is known about ADK's contribution to the regulation of plant growth ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.106.084798
更新日期:2006-10-01 00:00:00
abstract::The activity of the maize (Zea mays) florigen gene ZEA CENTRORADIALIS8 (ZCN8) is associated with the floral transition in both day-neutral temperate maize and short-day (SD)-requiring tropical maize. We analyzed transcription and chromatin modifications at the ZCN8 locus and its nearly identical paralog ZCN7 during th...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.15.00535
更新日期:2015-08-01 00:00:00
abstract::Ion transport, measured using double-barreled micropipettes to obtain current-voltage relations, was examined in Arabidopsis thaliana root hairs that continued tip growth and cytoplasmic streaming after impalement with the micropipette. To do this required in situ measurements with no handling of the seedlings to avoi...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.97.4.1527
更新日期:1991-12-01 00:00:00
abstract::Glycine decarboxylase (GDC) plays an important role in the photorespiratory metabolism of plants. GDC is composed of four subunits (P, H, L, and T) with the P-subunit (GLDP) serving as the actual decarboxylating unit. In C(3) plants, GDC can be found in all photosynthetic cells, whereas in leaves of C(3)-C(4) intermed...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.107.114462
更新日期:2008-04-01 00:00:00
abstract::Young cabbage plants (Brassica oleracea L. var. capitata) that were exposed to an atmosphere at 50% relative humidity transpired freely and accumulated significant quantities of (45)Ca in the leaves. Plants that were enclosed by plastic bags to stop transpiration from all leaves exhibited guttation with the developmen...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.60.6.854
更新日期:1977-12-01 00:00:00
abstract::Freeze-thaw events can affect plant hydraulics by inducing embolism. This study analyzed the effect of temperature during the freezing process on hydraulic conductivity and ultrasonic emissions (UE). Stems of 10 angiosperms were dehydrated to a water potential at 12% percentage loss of hydraulic conductivity (PLC) and...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.113.228403
更新日期:2014-02-01 00:00:00
abstract::We previously cloned and sequenced a cDNA encoding soybean ferric leghemoglobin reductase (FLbR), an enzyme postulated to play an important role in maintaining leghemoglobin in a functional ferrous state in nitrogen-fixing root nodules. This cDNA was sub-cloned into an expression plasmid, pTrcHis C, and overexpressed ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.106.1.203
更新日期:1994-09-01 00:00:00
abstract::Formation of the riboside-5'-monophosphate is a general feature of the metabolism of cytokinins in plants. As part of a study of the biological significance of the nucleotide form of cytokinins, we analyzed a mutant of Arabidopsis thaliana deficient in adenine phosphoribosyltransferase (APRT) activity for its ability ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.95.3.900
更新日期:1991-03-01 00:00:00
abstract::Sodium dodecyl sulfate-polyacrylamide gel electrophoretic analysis of the starchy endosperm protein of rice (Oryza sativa L. Japonica cv Koshihikari) during seed development confirmed that storage protein begins to accumulate about 5 days after flowering. Two polypeptide groups, 22 to 23 and 37 to 39 kilodaltons, the ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.70.4.1094
更新日期:1982-10-01 00:00:00
abstract::Chlorophyll a fluorescence of Synechococcus UTEX 625 was quenched during the transport of inorganic carbon, even when CO(2) fixation was inhibited by iodoacetamide. Measurements with a pulse modulation fluorometer showed that at least 75% of the quenching was due to oxidation of Q(a), the primary acceptor of photosyst...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.88.1.6
更新日期:1988-09-01 00:00:00
abstract::Sections of sugar beet (Beta vulgaris L.) taproot were incubated in various concentrations of mannitol. At 0.4, 0.6, and 0.8 molar, the membrane electrical potential difference (E(m)) averaged about -130 millivolts; at 0.2 molar, about -90 millivolts; and at 0 molar, between -60 and -80 millivolts. Additions of 10 mil...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.82.4.1148
更新日期:1986-12-01 00:00:00
abstract::Structural and metabolic features of the seedcoats of the developing pea seed indicate that events in the cells of the seedcoats are of major importance in controlling the development of the embryo. Information has been obtained on the distribution of N and P constituents of the seedcoats, embryo sac liquid, and the c...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.64.5.763
更新日期:1979-11-01 00:00:00
abstract::The O(2) concentration in intact and osmotically disrupted isolated spinach (Spinacia oleracea, L.) chloroplasts during photosynthesis was estimated. The chloroplasts were allowed to reduce 3-phosphoglycerate, CO(2), or ferricyanide in light until the rate of O(2) production was linear. When the light was turned off O...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.60.6.903
更新日期:1977-12-01 00:00:00
abstract::Inactivation of inward-rectifying K+ channels (IK,in) by a rise in cytosolic free [Ca2+] ([Ca2+]i) is a key event leading to solute loss from guard cells and stomatal closure. However, [Ca2+]i action on IK,in has never been quantified, nor are its origins well understood. We used membrane voltage to manipulate [Ca2+]i...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.119.1.277
更新日期:1999-01-01 00:00:00
abstract::Of the eight intermediates associated with the two pathways of UDP-d-glucuronic acid biosynthesis found in plants, only d-glucuronic acid inhibited myo-inositol 1-phosphate synthase (EC 5.5.1.4), formerly referred to as d-glucose 6-phosphate cycloaldolase. Inhibition was competitive. An attempt to demonstrate over-all...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.54.3.368
更新日期:1974-09-01 00:00:00
abstract::Recent advances in the development of methods for measuring cytoplasmic Ca(2+) levels in higher plant cells are discussed. Emphasis is placed on the new generation of Ca(2+)-sensitive fluorescent dyes particularly fura-2 and indo-1. These dyes offer many advantages for the measurement of cytosolic Ca(2+) levels. They ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.93.3.841
更新日期:1990-07-01 00:00:00
abstract::The role of polygalacturonase in leaf abscission was studied in explants of Phaseolus vulgaris L. cv. Red Kidney. Bean polygalacturonase was partially characterized and comparisons were made between the bean enzyme and previously reported higher plant polygalacturonases. Polygalacturonase isolated from bean leaf absci...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.63.6.1133
更新日期:1979-06-01 00:00:00
abstract::The first committed step in the conversion of cycloartenol into Delta(5) C24-alkyl sterols in plants is catalyzed by an S-adenosyl-methionine-dependent sterol-C24-methyltransferase type 1 (SMT1). We report the consequences of overexpressing SMT1 in tobacco (Nicotiana tabacum), under control of either the constitutive ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.004226
更新日期:2002-09-01 00:00:00
abstract::The incorporation of adenosine-8-(14)C into adenosine cyclic 3':5'-monophosphate in coleoptile-first leaf segments of Avena sativa L. was investigated. Homogenates of segments incubated in adenosine-8-(14)C for either 4 or 10 hours were partially purified by thin layer chromatography followed by paper electrophoresis....
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.55.2.346
更新日期:1975-02-01 00:00:00
abstract::Calcium flux in sunflower (Helianthus annuus L. cv Russian mammoth) hypocotyl was measured with a Ca(2+) electrode as the increase or decrease in Ca(2+) in an aqueous solution (10 micromolar CaCl(2)) in contact with either the basal or apical end of 20 millimeter segments. Ca(2+) efflux was significantly higher at the...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.81.2.408
更新日期:1986-06-01 00:00:00
abstract::The cytochrome b(6)f (Cyt b(6)f) complex in flowering plants contains nine conserved subunits, of which three, PetG, PetL, and PetN, are bitopic plastid-encoded low-molecular-weight proteins of largely unknown function. Homoplastomic knockout lines of the three genes have been generated in tobacco (Nicotiana tabacum '...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.107.100131
更新日期:2007-08-01 00:00:00