Measuring intracellular ca levels in plant cells using the fluorescent probes, indo-1 and fura-2 : progress and prospects.

Abstract:

:Recent advances in the development of methods for measuring cytoplasmic Ca(2+) levels in higher plant cells are discussed. Emphasis is placed on the new generation of Ca(2+)-sensitive fluorescent dyes particularly fura-2 and indo-1. These dyes offer many advantages for the measurement of cytosolic Ca(2+) levels. They can be introduced into cells in a nonintrusive manner, their K(d) for Ca(2+) matches plant cell cytoplasmic Ca(2+) levels, and shifts in their emission (indo-1) or excitation (fura-2) spectra following Ca(2+) binding permit accurate quantitation of Ca(2+) activities. Examples of cytoplasmic Ca(2+) levels measured in plants with fura-2 and indo-1 are presented, and the prospects for applying more advanced technologies to fluorescent dye measurement are discussed.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Bush DS,Jones RL

doi

10.1104/pp.93.3.841

subject

Has Abstract

pub_date

1990-07-01 00:00:00

pages

841-5

issue

3

eissn

0032-0889

issn

1532-2548

journal_volume

93

pub_type

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