Abstract:
:Recent advances in the development of methods for measuring cytoplasmic Ca(2+) levels in higher plant cells are discussed. Emphasis is placed on the new generation of Ca(2+)-sensitive fluorescent dyes particularly fura-2 and indo-1. These dyes offer many advantages for the measurement of cytosolic Ca(2+) levels. They can be introduced into cells in a nonintrusive manner, their K(d) for Ca(2+) matches plant cell cytoplasmic Ca(2+) levels, and shifts in their emission (indo-1) or excitation (fura-2) spectra following Ca(2+) binding permit accurate quantitation of Ca(2+) activities. Examples of cytoplasmic Ca(2+) levels measured in plants with fura-2 and indo-1 are presented, and the prospects for applying more advanced technologies to fluorescent dye measurement are discussed.
journal_name
Plant Physioljournal_title
Plant physiologyauthors
Bush DS,Jones RLdoi
10.1104/pp.93.3.841subject
Has Abstractpub_date
1990-07-01 00:00:00pages
841-5issue
3eissn
0032-0889issn
1532-2548journal_volume
93pub_type
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