Production of recombinant proteins in tobacco guttation fluid.

abstract：:Mitochondria were isolated from excised coleoptiles of hardened and nonhardened winter rye (Secale cereale L. cv. Puma) seedlings which had been frozen extracellularly to different temperatures. No significant differences in the respiratory functions (ADP/O and respiratory control) were observed between mitochondria i...

journal_title：Plant physiology

authors： Singh J,de la Roche AI,Siminovitch D

更新日期：1977-11-01 00:00:00

abstract：:Root respiration associated with symbiotic fixation in soybean (Glycine max [L.] Merr.) was estimated by four methods.Averaged over the life of the plant, the root respires 5.8 milligrams C per milligram N accumulated from fixation. When nitrogenase (C(2)H(2)) activity and root respiration were decreased by treating r...

journal_title：Plant physiology

authors： Patterson TG,Larue TA

更新日期：1983-07-01 00:00:00

abstract：:The functions of mitochondria during leaf senescence, a type of programmed cell death aimed at the massive retrieval of nutrients from the senescing organ to the rest of the plant, remain elusive. Here, combining experimental and analytical approaches, we showed that mitochondrial integrity in Arabidopsis (Arabidopsis...

journal_title：Plant physiology

authors： Chrobok D,Law SR,Brouwer B,Lindén P,Ziolkowska A,Liebsch D,Narsai R,Szal B,Moritz T,Rouhier N,Whelan J,Gardeström P,Keech O

更新日期：2016-12-01 00:00:00

abstract：:We have found nuclear, recessive mutants in Zea mays L. where assembly of the major chlorophyll (a/b) light-harvesting complex (LHC) was not delayed relative to most other thylakoid protein complexes during thylakoid biogenesis. This contrasts with the normal development of maize chloroplasts (NR Baker, R Leech 1977 P...

journal_title：Plant physiology

authors： Polacco ML,Chang MT,Neuffer MG

更新日期：1985-04-01 00:00:00

abstract：:Alfalfa was grown hydroponically in 0, 0.6, and 4.8 millimolar K in order to determine the influence of tissue level of K on photosynthesis, dark respiration, photorespiration, stomatal and mesophyll resistance to CO(2), photosystem I and II activity, and synthesis and activity of ribulose 1,5-bisphosphate carboxylase...

journal_title：Plant physiology

authors： Peoples TR,Koch DW

更新日期：1979-05-01 00:00:00

abstract：:Through a single genetic transformation in onion (Allium cepa), a crop recalcitrant to genetic transformation, we suppressed the lachrymatory factor synthase gene using RNA interference silencing in six plants. This reduced lachrymatory synthase activity by up to 1,544-fold, so that when wounded the onions produced si...

journal_title：Plant physiology

authors： Eady CC,Kamoi T,Kato M,Porter NG,Davis S,Shaw M,Kamoi A,Imai S

更新日期：2008-08-01 00:00:00

abstract：:Electron micrographs are presented which establish the identity of the components of the 3 major bands observed after sucrose density centrifugation of the crude particulate fraction from the endosperm of germinating castor bean seedlings. These are: mitochondria (density 1.19 g/cc), proplastids (density 1.23 g/cc) an...

journal_title：Plant physiology

authors： Breidenbach RW,Kahn A,Beevers H

更新日期：1968-05-01 00:00:00

abstract：:The subcellular locations of Ca(2+)-ATPases in the membranes of cauliflower (Brassica oleracea L.) inflorescences were investigated. After continuous sucrose gradient centrifugation a 111-kD calmodulin (CaM)-stimulated and caM-binding Ca(2+)-ATPase (BCA1; P. Askerlund [1996] Plant Physiol 110: 913-922; S. Malmström, P...

journal_title：Plant physiology

authors： Askerlund P

更新日期：1997-07-01 00:00:00

abstract：:Plants undergoing heat and low-CO2 stresses emit large amounts of volatile isoprenoids compared with those in stress-free conditions. One hypothesis posits that the balance between reducing power availability and its use in carbon assimilation determines constitutive isoprenoid emission rates in plants and potentially...

journal_title：Plant physiology

authors： Dani KG,Jamie IM,Prentice IC,Atwell BJ

更新日期：2014-10-01 00:00:00

abstract：:The trafficking and function of cell surface proteins in eukaryotic cells may require association with detergent-resistant sphingolipid- and sterol-rich membrane domains. The aim of this work was to obtain evidence for lipid domain phenomena in plant membranes. A protocol to prepare Triton X-100 detergent-resistant me...

journal_title：Plant physiology

authors： Borner GH,Sherrier DJ,Weimar T,Michaelson LV,Hawkins ND,Macaskill A,Napier JA,Beale MH,Lilley KS,Dupree P

更新日期：2005-01-01 00:00:00

abstract：:Crown galls induced by infection of tobacco plants with Agrobacterium tumefaciens strain C58-Cl(pTiB6S3) were excised and cultured in vitro. After about one year of culture on medium-lacking phytohormones, two noncloned lines spontaneously formed shoots. Leaf explants from shoots of tumor-line T5 were capable of growi...

journal_title：Plant physiology

authors： Owens LD

更新日期：1982-01-01 00:00:00

abstract：:To assess the variation in nutrient homeostasis in oilseed rape and to identify the genes responsible for this variation, we determined foliar anion levels in a diversity panel of Brassica napus accessions, 84 of which had been genotyped previously using messenger RNA sequencing. We applied associative transcriptomics...

journal_title：Plant physiology

authors： Koprivova A,Harper AL,Trick M,Bancroft I,Kopriva S

更新日期：2014-09-01 00:00:00

abstract：:Here, we monitor the effects of ectopic overexpression of genes for pea asparagine synthetase (AS1) in transgenic tobacco (Nicotiana tabacum). The AS genes of pea and tobacco are normally expressed only during the dark phase of the diurnal growth cycle and specifically in phloem cells. A hybrid gene was constructed in...

journal_title：Plant physiology

authors： Brears T,Liu C,Knight TJ,Coruzzi GM

更新日期：1993-12-01 00:00:00

abstract：:The role of roots in the enhancement of cytokinin content and leaf growth of Phaseolus vulgaris plants after decapitation and partial defoliation was investigated. Partial excision of the roots of plants which were decapitated above the primary leaf node resulted in a reduction of leaf growth and soluble proteins accu...

journal_title：Plant physiology

authors： Carmi A,Staden JV

更新日期：1983-09-01 00:00:00

abstract：:A population of M2 seedlings of Arabidopsis thaliana was screened for mutants that were insensitive to the ethylene precursor 1-aminocyclopropane-1-carboxylate (ACC). Several independent lines were obtained and proved insensitive to both ACC and ethylene. Two lines were identified as alleles of a single recessive muta...

journal_title：Plant physiology

authors： Van Der Straeten D,Djudzman A,Van Caeneghem W,Smalle J,Van Montagu M

更新日期：1993-06-01 00:00:00

abstract：:Chlorophyll a fluorescence of Synechococcus UTEX 625 was quenched during the transport of inorganic carbon, even when CO(2) fixation was inhibited by iodoacetamide. Measurements with a pulse modulation fluorometer showed that at least 75% of the quenching was due to oxidation of Q(a), the primary acceptor of photosyst...

journal_title：Plant physiology

authors： Miller AG,Espie GS,Canvin DT

更新日期：1988-09-01 00:00:00

abstract：:A pulse of red light acting through phytochrome accelerates the formation of chlorophyll upon subsequent transfer of dark-grown seedlings to continuous white light. Specific antibodies were used to follow the accumulation of representative subunits of the major photosynthetic complexes during greening of seedlings of ...

journal_title：Plant physiology

authors： Pauncz Y,Gepstein S,Horwitz BA

更新日期：1992-12-01 00:00:00

abstract：:Folates typically have gamma-linked polyglutamyl tails that make them better enzyme substrates and worse transport substrates than the unglutamylated forms. The tail can be shortened or removed by the vacuolar enzyme gamma-glutamyl hydrolase (GGH). It is known that GGH is active only as a dimer and that plants can hav...

journal_title：Plant physiology

authors： Akhtar TA,McQuinn RP,Naponelli V,Gregory JF 3rd,Giovannoni JJ,Hanson AD

更新日期：2008-10-01 00:00:00

abstract：:The presence of multiple forms of alpha-amylase in gibberellic acid-treated embryoless barley half-seeds was demonstrated by separation on diethylaminoethyl-Sephadex and isoelectric focusing polyacrylamide gel disc electrophoresis. Two major alpha-amylase fractions (A and B), each consisting of two to three isozyme co...

journal_title：Plant physiology

authors： Tanaka Y,Akazawa T

更新日期：1970-10-01 00:00:00

abstract：:A high affinity cAMP-binding protein (cABP II) was purified to homogeneity from wheat germ. The apparent molecular weight of cABP II, as determined from gel exclusion chromatography, is 5.2 x 10(5) (at low ionic strength) and 2.8 x 10(5) (at high ionic strength). One polypeptide subunit (molecular weight, 80,000) was ...

journal_title：Plant physiology

authors： Polya GM,Bowman JA

更新日期：1981-09-01 00:00:00

abstract：:Cell-free preparations from seeds of Marah macrocarpus L. and Malus domestica L. catalyzed the conversion of gibberellin A(9) (GA(9)) and 2,3-dehydroGA(9) to GA(7); GA(9) was also metabolized to GA(4) in a branch pathway. The preparation from Marah seeds also metabolized GA(5) to GA(3) in high yield; GA(6) was a minor...

journal_title：Plant physiology

authors： Albone KS,Gaskin P,Macmillan J,Phinney BO,Willis CL

更新日期：1990-09-01 00:00:00

abstract：:Cell-free extracts from gibberellic acid-treated barley (Hordeum vulgare L. cv. Himalaya) aleurone layers show phosphorylcholine glyceride transferase activity greater than that from control layers. The increase in activity is not prevented by a mixture of amino acid analogs nor by cordycepin under conditions in which...

journal_title：Plant physiology

authors： Ben-Tal Y

更新日期：1974-12-01 00:00:00

abstract：:The herbicide Sandoz 6706 (4-chloro-5-(dimethylamino)-2-alpha,alpha,alpha, (trifluoro-m-tolyl)-3(2H)-pyridazinone), when applied as a preplant soil treatment at a concentration of 0.05 mug/g reduced the content of beta-carotene and chlorophylls in 21-day-old wheat seedlings (Triticum aestivum L.) by 55% and 29%, respe...

journal_title：Plant physiology

authors： Ben-Aziz A,Koren E

更新日期：1974-12-01 00:00:00

abstract：:Lipases are involved in the generation of jasmonates, which regulate responses to biotic and abiotic stresses. Two sn-1-specific acyl hydrolases, DEFECTIVE IN ANTHER DEHISCENCE1 (DAD1) and DONGLE (DGL), have been reported to be localized in plastids and to be essential and sufficient for jasmonate biosynthesis in Arab...

journal_title：Plant physiology

authors： Ellinger D,Stingl N,Kubigsteltig II,Bals T,Juenger M,Pollmann S,Berger S,Schuenemann D,Mueller MJ

更新日期：2010-05-01 00:00:00

abstract：:We previously reported (A Reinero, RN Beachy 1986 Plant Mol Biol 6:291-301) that coat protein (CP) of tobacco mosaic virus (TMV) accumulates in chloroplasts of systemically infected leaves. To determine the significance of such interaction we examined electron transport rates in chloroplasts containing different level...

journal_title：Plant physiology

authors： Reinero A,Beachy RN

更新日期：1989-01-01 00:00:00

abstract：:The distribution of polysomes in the cells of the endosperm of very young (48 hours of germination) seedlings of castor bean (Ricinus communis var. Hale) has been examined. Seedlings exposed to 100 micromolar gibberellin A(3) for periods up to 24 hours showed increased numbers of polysomes associated with endomembrane...

journal_title：Plant physiology

authors： González E

更新日期：1980-02-01 00:00:00

abstract：:Cold hardiness of leaf blades, leaf sheaths, culms, rhizomes, and leaf buds in wintering Sasa senanensis (Fr. et Sav.) Rehder, a dwarf bamboo, was studied paying special attention to the types of resistance mechanisms which were determined with differential thermal analysis. Coincidence of LT(25) (lethal temperature a...

journal_title：Plant physiology

authors： Ishikawa M

更新日期：1984-05-01 00:00:00

abstract：:Salinity is an environmental stress that causes decline in crop yield. Avicennia officinalis and other mangroves have adaptations such as ultrafiltration at the roots aided by apoplastic cell wall barriers to thrive in saline conditions. We studied a cytochrome P450 gene from A. officinalis, AoCYP94B1, and its putativ...

journal_title：Plant physiology

authors： Krishnamurthy P,Vishal B,Ho WJ,Lok FCJ,Lee FSM,Kumar PP

更新日期：2020-12-01 00:00:00

abstract：:Endo-polygalacturonase (PG) was purified from a commercial preparation of Aspergillus niger pectinase by means of carboxymethylcellulose chromatography, preparative isoelectric focusing, and gel permeation through Sephadex G-50. The enzyme was electrophoretically homogeneous and consisted of a single polypeptide chain...

journal_title：Plant physiology

authors： Cervone F,De Lorenzo G,Degrà L,Salvi G

更新日期：1987-11-01 00:00:00

abstract：:Germination potential in nondormant, upper cocklebur (Xanthium pensylvanicum Wallr.) seeds, which were incapable of germinating under constant temperatures below 25 C in air, was increased by exposure to diurnally alternating temperatures. The cocklebur seeds failed to respond to the temperature fluctuations in the be...

journal_title：Plant physiology

authors： Esashi Y,Tsukada Y

更新日期：1978-03-01 00:00:00