Silencing onion lachrymatory factor synthase causes a significant change in the sulfur secondary metabolite profile.

Abstract:

:Through a single genetic transformation in onion (Allium cepa), a crop recalcitrant to genetic transformation, we suppressed the lachrymatory factor synthase gene using RNA interference silencing in six plants. This reduced lachrymatory synthase activity by up to 1,544-fold, so that when wounded the onions produced significantly reduced levels of tear-inducing lachrymatory factor. We then confirmed, through a novel colorimetric assay, that this silencing had shifted the trans-S-1-propenyl-l-cysteine sulfoxide breakdown pathway so that more 1-propenyl sulfenic acid was converted into di-1-propenyl thiosulfinate. A consequence of this raised thiosulfinate level was a marked increase in the downstream production of a nonenzymatically produced zwiebelane isomer and other volatile sulfur compounds, di-1-propenyl disulfide and 2-mercapto-3,4-dimethyl-2,3-dihydrothiophene, which had previously been reported in trace amounts or had not been detected in onion. The consequences of this dramatic simultaneous down- and up-regulation of secondary sulfur products on the health and flavor attributes of the onion are discussed.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Eady CC,Kamoi T,Kato M,Porter NG,Davis S,Shaw M,Kamoi A,Imai S

doi

10.1104/pp.108.123273

subject

Has Abstract

pub_date

2008-08-01 00:00:00

pages

2096-106

issue

4

eissn

0032-0889

issn

1532-2548

pii

pp.108.123273

journal_volume

147

pub_type

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