Abstract:
:Through a single genetic transformation in onion (Allium cepa), a crop recalcitrant to genetic transformation, we suppressed the lachrymatory factor synthase gene using RNA interference silencing in six plants. This reduced lachrymatory synthase activity by up to 1,544-fold, so that when wounded the onions produced significantly reduced levels of tear-inducing lachrymatory factor. We then confirmed, through a novel colorimetric assay, that this silencing had shifted the trans-S-1-propenyl-l-cysteine sulfoxide breakdown pathway so that more 1-propenyl sulfenic acid was converted into di-1-propenyl thiosulfinate. A consequence of this raised thiosulfinate level was a marked increase in the downstream production of a nonenzymatically produced zwiebelane isomer and other volatile sulfur compounds, di-1-propenyl disulfide and 2-mercapto-3,4-dimethyl-2,3-dihydrothiophene, which had previously been reported in trace amounts or had not been detected in onion. The consequences of this dramatic simultaneous down- and up-regulation of secondary sulfur products on the health and flavor attributes of the onion are discussed.
journal_name
Plant Physioljournal_title
Plant physiologyauthors
Eady CC,Kamoi T,Kato M,Porter NG,Davis S,Shaw M,Kamoi A,Imai Sdoi
10.1104/pp.108.123273subject
Has Abstractpub_date
2008-08-01 00:00:00pages
2096-106issue
4eissn
0032-0889issn
1532-2548pii
pp.108.123273journal_volume
147pub_type
杂志文章相关文献
PLANT PHYSIOLOGY文献大全abstract::Starch accumulation and sucrose synthesis and export were measured in leaves of sugar beet (Beta vulgaris L.) during a period of prolonged irradiance in which illumination was extended beyond the usual 14-hour day period. During much of the 14-hour day period, approximately 50% of the newly fixed carbon was distribute...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.99.4.1393
更新日期:1992-08-01 00:00:00
abstract::Excised roots of the tomato species, Lycopersicon esculentum Mill. cv Walter (the commercial species) and of Lycopersicon cheesmanii ssp. minor (Hook.) C.H. Mull. (a wild species from the Galapagos Islands), were used in comparative studies of their absorption of K(+) and Na(+). Uptake of (86)Rb-labeled K(+) and (22)N...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.79.4.1064
更新日期:1985-12-01 00:00:00
abstract::Meristematic tissues from rye (Secale cereale) and oat (Avena sativa) were studied in an isothermal calorimeter at -3 degrees C. When the frozen tissue was placed in the calorimeter, the pressure increased within 4 d to 25 and 9 kPa above ambient pressure in the sample vessels containing crowns of rye and oat, respect...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.122.3.861
更新日期:2000-03-01 00:00:00
abstract::Auxin biosynthesis was analyzed in a maize (Zea mays) kernel culture system in which the seeds develop under physiological conditions similar to the in vivo situation. This system was modified for precursor feeding experiments. Tryptophan (Trp) is efficiently incorporated into indole-3-acetic acid (IAA) with retention...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.123.3.1109
更新日期:2000-07-01 00:00:00
abstract::The Dof (DNA binding with one finger) transcriptional activator rice (Oryza sativa) prolamin box binding factor (RPBF), which is involved in gene regulation of rice seed storage proteins, has been isolated from rice cDNA expressed sequence tag clones containing the conserved Dof. RPBF is found as a single gene per hap...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.106.082826
更新日期:2006-08-01 00:00:00
abstract::We have used (13)C-labeled sugars and nuclear magnetic resonance (NMR) spectrometry to study the metabolic pathway of starch biosynthesis in developing wheat grain (Triticum aestivum cv Mardler). Our aim was to examine the extent of redistribution of (13)C between carbons atoms 1 and 6 of [1-(13)C] or [6-(13)C]glucose...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.87.2.311
更新日期:1988-06-01 00:00:00
abstract::Photosynthetic (14)CO(2) fixation, [(14)C]glycolate formation, and the decarboxylation of [1-(14)C]glycolate and [1-(14)C]glycine by leaf mesophyll protoplasts isolated from isogenic diploid and tetraploid cultivars of ryegrass (Lolium perenne L.) were examined. The per cent O(2) inhibition of photosynthesis in protop...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.65.3.489
更新日期:1980-03-01 00:00:00
abstract::To survive environmental challenges, plants have evolved tightly regulated response networks, including a rapid and transient general stress response (GSR), followed by well-studied stress-specific responses. The mechanisms underpinning the GSR have remained elusive, but a functional cis-element, the rapid stress resp...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.114.245944
更新日期:2014-10-01 00:00:00
abstract::Acropetal mass flow of water is demonstrated in two submerged angiosperms, Lobelia dortmanna L. and Sparganium emersum Rehman by means of guttation measurements. Transpiration is absent in truly submerged plants, but the presence of guttation verifies that long-distance water transport takes place. Use of tritiated wa...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.103.4.1369
更新日期:1993-12-01 00:00:00
abstract::Various sterols and related steroids were tested for their ability to influence ethanol-induced electrolyte leakage from Hordeum vulgare roots. Cholesterol had the greatest influence and, depending on concentration, it stimulated or inhibited the loss of electrolyte. Cholesterol, however, was ineffective if the roots ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.54.4.624
更新日期:1974-10-01 00:00:00
abstract::Through a biochemical and a genetic approach, we have identified several plant genes encoding methylthioribose (MTR) kinase, an enzyme involved in recycling of methionine through the methylthioadenosine (MTA) cycle. OsMTK1, an MTR kinase from rice (Oryza sativa), is 48.6 kD in size and shows cooperative kinetics with ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.104.053587
更新日期:2004-12-01 00:00:00
abstract::Fructose 2,6-bisphosphate inhibits phosphoglucomutase noncompetitively with respect to the cofactor glucose 1,6-bisphosphate. Previous studies from our laboratory had shown that phosphoglucomutase was activated by fructose 2,6-bisphosphate in the absence of added glucose 1,6-bisphosphate. The fructose 2,6-bisphosphate...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.88.4.980
更新日期:1988-12-01 00:00:00
abstract::Four legume species (four genera) were examined and found to contain hemagglutinins with properties similar to those which we have previously described for the enzymic hemagglutinin in Vigna radiata. Examination of extracts by gel filtration and ion exchange chromatography showed that an alpha-galactosidase activity e...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.65.4.618
更新日期:1980-04-01 00:00:00
abstract::Growth at an elevated CO(2) concentration resulted in an enhanced capacity for soybean (Glycine max L. Merr. cv Bragg) leaflet photosynthesis. Plants were grown from seed in outdoor controlled-environment chambers under natural solar irradiance. Photosynthetic rates, measured during the seed filling stage, were up to ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.88.4.1310
更新日期:1988-12-01 00:00:00
abstract::This study aims to elucidate the molecular mechanism of an alternative electron flow (AEF) functioning under suppressed (CO2-limited) photosynthesis in the cyanobacterium Synechocystis sp. PCC 6803. Photosynthetic linear electron flow, evaluated as the quantum yield of photosystem II [Y(II)], reaches a maximum shortly...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.114.249987
更新日期:2015-02-01 00:00:00
abstract::The pattern of endoproteolytic activities occurring during wheat (Triticum aestivum, cultivar Chinese Spring) grain development was investigated. Total endoprotease activity, assayed in solution with azocasein as a substrate, increased during the early stages of grain development to reach a maximum at 15 d postanthesi...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.112.3.1211
更新日期:1996-11-01 00:00:00
abstract::We have purified the protein for the enzyme sucrose phosphate synthase (SPS) from corn (Zea mays) leaves. Partially purified SPS protein was used to generate specific monoclonal antibodies. The following immunoaffinity chromatography allowed the isolation of pure SPS protein. The apparent molecular mass of the SPS pol...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.96.2.473
更新日期:1991-06-01 00:00:00
abstract::Dimethylallyl diphosphate (DMADP) and geranyl diphosphate (GDP) are the last precursors of isoprene and monoterpenes emitted by leaves, respectively. DMADP and GDP pools were measured in leaves of plants emitting isoprene (Populus alba), monoterpenes (Quercus ilex and Mentha piperita), or nonemitting isoprenoids (Prun...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.105.073213
更新日期:2006-06-01 00:00:00
abstract::The thermophilic blue-green alga Synechococcus lividus was grown at 55 and 38 C. Arrhenius plots of the transient reduction of cytochrome during actinic illumination with light that excited both pigment systems revealed breaks near 43 and 26 C for cells grown at 55 C. In cells grown at 38 C these breaks occurred near ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.63.3.524
更新日期:1979-03-01 00:00:00
abstract::Although cytosolic free Ca(2+) mobilization induced by microbe/pathogen-associated molecular patterns is postulated to play a pivotal role in innate immunity in plants, the molecular links between Ca(2+) and downstream defense responses still remain largely unknown. Calcineurin B-like proteins (CBLs) act as Ca(2+) sen...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.109.151852
更新日期:2010-06-01 00:00:00
abstract::Ethylene-responsive element binding factors (ERF) proteins are plant-specific transcription factors, many of which have been linked to stress responses. We have identified four Arabidopsis ERF genes whose expression was specifically induced by avirulent and virulent strains of the bacterial pathogen Pseudomonas syring...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.010862
更新日期:2002-04-01 00:00:00
abstract::Responses of tobacco (Nicotiana tabacum) suspension cells to Cd and Zn were studied in the presence and absence of ligand of Cd-peptide in order to understand the role of this peptide versus other mechanisms in Cd and Zn accumulation and accommodation in plants. With 45 micromolar Cd and 300 micromolar Zn (non-growth-...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.91.2.780
更新日期:1989-10-01 00:00:00
abstract::The dark respiration rate of discs from fully expanded tobacco leaves (Nicotiana tabacum) increased linearly with decreasing diameter, the relative increase being independent of leaf age. The wound respiration responsible for this situation reached a plateau within 15 minutes of excision. Metabolite analysis gave evid...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.57.1.80
更新日期:1976-01-01 00:00:00
abstract::The isotope competition method with glucose-U-(14)C as a carbon source has been used to determine whether or not selected compounds contribute carbon to the biosynthesis of protein amino acids in cells derived from Paul's Scarlet Rose. Of 48 compounds tested, 15 contributed carbon to protein amino acids. The results s...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.42.7.941
更新日期:1967-07-01 00:00:00
abstract::The subcellular site of the posttranslational modification of alpha-amylase was investigated in aleurone layers of barley (Hordeum vulgare L. cv Himalaya). Aleurone layers of Himalaya barley synthesize and secrete two groups of alpha-amylase isoforms, referred to as low-isoelectric point (low-pl) or HAMY1 and high-pl ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.98.3.1080
更新日期:1992-03-01 00:00:00
abstract::Sodium dodecyl sulfate-polyacrylamide gel electrophoretic analysis of the starchy endosperm protein of rice (Oryza sativa L. Japonica cv Koshihikari) during seed development confirmed that storage protein begins to accumulate about 5 days after flowering. Two polypeptide groups, 22 to 23 and 37 to 39 kilodaltons, the ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.70.4.1094
更新日期:1982-10-01 00:00:00
abstract::Greenhouse and field studies examined the effect of flower or seedhead removal on leaf senescence and associated changes in sunflower (Helianthus annuus L.) plants. At intervals during seed development, selected leaves (leaves 6 through 8 from the top in the greenhouse and leaf 7 from the top in the field) were harves...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.83.4.844
更新日期:1987-04-01 00:00:00
abstract::PIB-type ATPases transport heavy metal ions (Cu+, Cu2+, Zn2+, Cd2+, Co2+, etc.) across biological membranes. Several members of this subfamily are present in plants. Higher plants are the only eukaryotes where putative Zn(2+)-ATPases have been identified. We have cloned HMA2, a PIB-ATPase present in Arabidopsis (Arabi...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.104.046292
更新日期:2004-11-01 00:00:00
abstract::The plant cuticle is an extracellular hydrophobic layer that covers the aerial epidermis of all land plants, providing protection against desiccation and external environmental stresses. The past decade has seen considerable progress in assembling models for the biosynthesis of its two major components, the polymer cu...
journal_title:Plant physiology
pub_type: 杂志文章,评审
doi:10.1104/pp.113.222737
更新日期:2013-09-01 00:00:00
abstract::The addition of primary amines to the growth medium of the unicellular green alga Chlamydomonas reinhardtii disrupts cell wall assembly in both vegetative and zygotic cells. Primary amines are competitive inhibitors of the protein-cross-linking activity of transglutaminases. Two independent assays for transglutaminase...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.121.3.1003
更新日期:1999-11-01 00:00:00