Abstract:
:Photosynthetic (14)CO(2) fixation, [(14)C]glycolate formation, and the decarboxylation of [1-(14)C]glycolate and [1-(14)C]glycine by leaf mesophyll protoplasts isolated from isogenic diploid and tetraploid cultivars of ryegrass (Lolium perenne L.) were examined. The per cent O(2) inhibition of photosynthesis in protoplasts from the tetraploid cultivar was less than that of the diploid line at both 21 and 49% O(2). Kinetic studies revealed that the K(m) (CO(2)) for photosynthesis by the diploid protoplasts was about twice that of the tetraploid line. In contrast, the K(i) (O(2)) for protoplast photosynthesis was similar in both cultivars, as was the potential for oxidizing glycolate and glycine to CO(2) via the photorespiratory carbon oxidation cycle. Although the maximal rates of glycolate accumulation by the isolated protoplasts in the presence of 21% O(2) and a glycolate oxidase inhibitor were similar in the two cultivars, the percentage of total fixed (14)C entering the [(14)C]glycolate pool and the ratio of the rate of [(14)C]glycolate formation to (14)CO(2) fixation at 21% O(2) and low pCO(2) were about two times greater in protoplasts and intact chloroplasts isolated from the diploid line compared to the tetraploid. These results fully support the recent observation that a doubling of ploidy in various ryegrass cultivars reduced the K(m) (CO(2)) of purified ribulose bisphosphate carboxylase-oxygenase by about one-half without affecting the K(i) (O(2)) (Garrett 1978 Nature 274: 913-915).
journal_name
Plant Physioljournal_title
Plant physiologyauthors
Rathnam CK,Chollet Rdoi
10.1104/pp.65.3.489subject
Has Abstractpub_date
1980-03-01 00:00:00pages
489-94issue
3eissn
0032-0889issn
1532-2548journal_volume
65pub_type
杂志文章相关文献
PLANT PHYSIOLOGY文献大全abstract::Why the leaves of many woody species accumulate anthocyanins prior to being shed has long puzzled biologists because it is unclear what effects anthocyanins may have on leaf function. Here, we provide evidence for red-osier dogwood (Cornus stolonifera) that anthocyanins form a pigment layer in the palisade mesophyll l...
journal_title:Plant physiology
pub_type: 杂志文章
doi:
更新日期:2001-10-01 00:00:00
abstract::The 7S seed storage protein (beta-conglycinin) of soybean (Glycine max [L]. Merr.) has three major subunits; alpha, alpha', and beta. Accumulation of the beta-subunit, but not the alpha- and alpha'-subunits, has been shown to be repressed by exogenously applied methionine to the immature cotyledon culture system (LP H...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.99.1.263
更新日期:1992-05-01 00:00:00
abstract::Extracellular calmodulin (ExtCaM) exerts multiple functions in animals and plants, but the mode of ExtCaM action is not well understood. In this paper, we provide evidence that ExtCaM stimulates a cascade of intracellular signaling events to regulate stomatal movement. Analysis of the changes of cytosolic free Ca2+ ([...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.104.047837
更新日期:2004-12-01 00:00:00
abstract::Among the four uppermost leaves of greenhouse-grown plants of Xanthium strumarium L. the content of abscisic acid per unit fresh or dry weight was highest in the youngest leaf and decreased gradually with increasing age of the leaves. Expressed per leaf, the second youngest leaf was richest in ABA; the amount of ABA p...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.58.2.169
更新日期:1976-08-01 00:00:00
abstract::Sections of sugar beet (Beta vulgaris L.) taproot were incubated in various concentrations of mannitol. At 0.4, 0.6, and 0.8 molar, the membrane electrical potential difference (E(m)) averaged about -130 millivolts; at 0.2 molar, about -90 millivolts; and at 0 molar, between -60 and -80 millivolts. Additions of 10 mil...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.82.4.1148
更新日期:1986-12-01 00:00:00
abstract::The cytosolic pools of glucose-1-phosphate (Glc-1-P) and glucose-6-phosphate are essential intermediates in several biosynthetic paths, including the formation of sucrose and cell wall constituents, and they are also linked to the cytosolic starch-related heteroglycans. In this work, structural features and biochemica...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.108.127969
更新日期:2008-11-01 00:00:00
abstract::DNA polymerase zeta catalytic subunit REV3 is known to play an important role in the repair of DNA damage induced by cross-linking and methylating agents. Here, we demonstrate that in Arabidopsis (Arabidopsis thaliana), the basic polymerase activity of REV3 is essential for resistance protection against these differen...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.15.00806
更新日期:2015-12-01 00:00:00
abstract::The uptake of cadmium by excised root tissue of barley (Hordeum vulgare L. cv. Arivat) was investigated with respect to kinetics, concentration, and interactions with various cations. The role of metabolism in Cd absorption was examined using a range of temperatures, anaerobic treatments, and chemical inhibitors. The ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.54.1.67
更新日期:1974-07-01 00:00:00
abstract::Microsomal membranes from suspension-cultured Catharanthus roseus cells possess an enzymic activity that catalyzes the ATP-dependent phosphorylation of phosphatidic acid (PA) to form diacylglycerol pyrophosphate (H. Behrbohm, J.B. Wissing [1993] FEBS Lett 315: 95-99). This enzyme activity, PA kinase, was purified and ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.102.4.1243
更新日期:1993-08-01 00:00:00
abstract::The mechanisms and the controlling factors of malate oxidation by mitochondria from leaves of Kalanchoë blossfeldiana Poelln. plants performing Crassulacean acid metabolism were investigated using Percollpurified mitochondria. The effects of pH and of various cofactors (ATP, NAD(+), coenzyme A) on malate dehydrogenase...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.81.4.1039
更新日期:1986-08-01 00:00:00
abstract::Vanadate (Na(3)VO(4)) selectively and reversibly affects the rhythmic movement of Albizzia julibrissin leaflets. Leaflets floated on 1 millimolar vanadate open at the same rate or more rapidly than controls, but closure is inhibited. After 6 to 24 hours incubation, the inhibition can be reversed by a 24-to 48-hour per...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.64.5.905
更新日期:1979-11-01 00:00:00
abstract::A novel, three-dimensional recording, vibrating probe was used for measuring the density and direction of the endogenous ionic current of cress roots (Lepidium sativum L.) bathed in low salt media (artificial pond water, APW). Roots submerged in regular APW and growing vertically show the following current pattern. Cu...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.100.1.16
更新日期:1992-09-01 00:00:00
abstract::The responses of redroot pigweed (Amaranthus retroflexus L.) seeds to nine ethylene concentrations between 0.5 and 50 microliters per liter were assessed at different temperatures and water potentials and in either continuous white light or darkness. Under all experimental treatments, the probit-transformed percentage...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.65.6.1149
更新日期:1980-06-01 00:00:00
abstract::The biosynthesis of the chlorophyll a/b binding protein associated with photosystem II (LHC-II) was characterized during light-induced greening of etiolated barley (Hordeum vulgare [L.] cv Boone), maize (Zea mays [L.] Pioneer 3148), pea (Pisum sativum [L.] cv Progress 9), and soybean (Glycine max [L.] Merr. cv Ransom ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.85.4.971
更新日期:1987-12-01 00:00:00
abstract::The ionic specificity of IAA-induced acidification by Avena coleoptiles was studied, using zwitterionic, presumably impermeant buffers. The acidification was almost totally dependent on divalent cations with an order of effectiveness of Ca(2+) >/= Sr(2+) > Mn(2+), Mg(2+); whereas other polyvalent cations tested were i...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.57.3.347
更新日期:1976-03-01 00:00:00
abstract::A technique is presented for the isolation of vacuoles from Sedum telephium L. leaves. Leaf material is digested enzymically to produce protoplasts rapidly which are partially lysed by gentle osmotic shock and the inclusion of 5 millimolar ethyleneglycol-bis (beta-aminoethyl ether)N,N'-tetraacetic acid in the wash med...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.66.3.379
更新日期:1980-09-01 00:00:00
abstract::Comparative genomics provides insight into the evolutionary dynamics that shape discrete sequences as well as whole genomes. To advance comparative genomics within the Brassicaceae, we have end sequenced 23,136 medium-sized insert clones from Boechera stricta, a wild relative of Arabidopsis (Arabidopsis thaliana). A s...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.105.073981
更新日期:2006-04-01 00:00:00
abstract::Glucose modulates plant metabolism, growth, and development. In Arabidopsis (Arabidopsis thaliana), Hexokinase1 (HXK1) is a glucose sensor that may trigger abscisic acid (ABA) synthesis and sensitivity to mediate glucose-induced inhibition of seedling development. Here, we show that the intensity of short-term respons...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.111.181743
更新日期:2011-10-01 00:00:00
abstract::We investigated the function of cell wall hydroxyproline-rich glycoproteins by observing the effects of a selective inhibitor of prolyl hydroxylase, 3,4-dehydro-L-proline (Dhp), on wall regeneration by Nicotiana tabacum mesophyll cell protoplasts. Protoplasts treated with micromolar concentrations of Dhp do not develo...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.104.2.747
更新日期:1994-02-01 00:00:00
abstract::To monitor the expression of T-DNA-tagged plant genes in vivo, a collection of 20,261 transgenic lines of Arabidopsis (Columbia-0) were generated with the promoter trap vector pTluc, which carries a promoterless firefly luc (luciferase) reporter gene linked to the right T-DNA border. By detection of bioluminescence in...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.103.027151
更新日期:2004-01-01 00:00:00
abstract::Senescence is a phase of leaf ontogeny marked by declining photosynthetic activity that, in soybean (Glycine max [L.] Merr.), is paralleled by a decline in chloroplast function. Soybean leaves have different patterns of decline in photosynthetic capacity and chloroplast function associated with nodal position and sink...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.101.1.105
更新日期:1993-01-01 00:00:00
abstract::Treatment of tobacco (Nicotiana tabacum) cell-suspension cultures with cryptogein, an elicitin protein from Phytophthora cryptogea, resulted in the release of a factor(s) that diffused through a 1000-D cutoff dialysis membrane and was capable of inducing sesquiterpene cyclase enzyme activity (a key phytoalexin biosynt...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.113.2.621
更新日期:1997-02-01 00:00:00
abstract::Thylakoids are the chloroplast internal membrane systems that house light-harvesting and electron transport reactions. Despite the important functions and well-studied constituents of thylakoids, the molecular mechanism of their development remains largely elusive. A recent genetic study has demonstrated that plastidi...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.109.151977
更新日期:2010-03-01 00:00:00
abstract::The sensitivity of lettuce (Lactuca sativa L. cv Grand Rapids) seeds to red light was reduced by NaCl concentrations which had no effect upon the germination of continuously illuminated seeds. The germination capacity of the seeds was fully restored by increased red light exposures. Indirect evidence indicates that Na...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.79.1.149
更新日期:1985-09-01 00:00:00
abstract::The effect of inorganic carbon (Ci) transport and accumulation on photosynthetic electron transport was studied in air-grown cells of the cyanobacterium Synechococcus UTEX 625. When the cells were depleted of Ci, linear photosynthetic electron flow was almost completely inhibited in the presence of the photosystem I (...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.114.4.1273
更新日期:1997-08-01 00:00:00
abstract::The induction of a dissolved inorganic carbon (DIC) accumulating mechanism in the two algal species Scenedesmus obliquus (WT) and Chlamydomonas reinhardtii (137 c+) was physiologically characterized by monitoring DIC uptake kinetics at a low and constant DIC concentration (120-140 micromolar), after transfer from high...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.87.2.437
更新日期:1988-06-01 00:00:00
abstract::Aroma contributes to the unique flavors of fruits and is important for fruit quality evaluation. Among the many volatiles in peach (Prunus persica) fruits, γ-decalactone has the greatest contribution to the characteristic peach aroma. Some peach cultivars have γ-decalactone contents that are too low to detect. Compari...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.19.00964
更新日期:2020-04-01 00:00:00
abstract::Changes in the physiological properties of the green alga Chlorella ellipsoidea (UTEX 20) were determined during adaptation from high CO2 to air. Cells of C. ellipsoidea, grown in high CO2, had an extremely low affinity for dissolved inorganic carbon (DIC). However, high-affinity DIC transport was induced rapidly afte...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.108.1.247
更新日期:1995-05-01 00:00:00
abstract::Annexins are a multigene family of calcium-dependent membrane-binding proteins that play important roles in plant cell signaling. Annexins are multifunctional proteins, and their function in plants is not comprehensively understood. Arabidopsis (Arabidopsis thaliana) annexins ANN1 and ANN2 are 64% identical in their p...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.18.00713
更新日期:2018-09-01 00:00:00
abstract::Polyadenylated RNA was isolated from maize leaves and translated in vitro. In agreement with a previous report by others, we found among the translation products a 110-kilodalton pyruvate orthophosphate dikinase (PPDK) precursor that is about 16 kilodaltons larger than the polypeptide isolated from cells. This maize P...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.78.4.807
更新日期:1985-08-01 00:00:00