Phosphatidate Kinase, a Novel Enzyme in Phospholipid Metabolism (Purification, Subcellular Localization, and Occurrence in the Plant Kingdom).

abstract：:A number of parameters associated with the isolation of plant mitochondria were carefully examined in the hope of optimizing the isolation technique. Dark-grown mung bean (Phaseolus aureus var. Jumbo) hypocotyls were used as the source of mitochondria. Necessary conditions for the isolation of tightly coupled plant mi...

journal_title：Plant physiology

authors： Ikuma H

更新日期：1970-06-01 00:00:00

abstract：:Low temperature is a seasonal abiotic stress that restricts native plant ranges and crop distributions. Two types of low-temperature stress can be distinguished: chilling and freezing. Much work has been done on the mechanisms by which chilling is sensed, but relatively little is known about how plants sense freezing....

journal_title：Plant physiology

authors： Barnes AC,Benning C,Roston RL

更新日期：2016-07-01 00:00:00

abstract：:Tyrosine decarboxylase (TYDC) is a common plant enzyme involved in the biosynthesis of numerous secondary metabolites, including hydroxycinnamic acid amides. Although a definite function has not yet been determined, amides have been proposed to form a physical barrier against pathogens because they are usually found a...

journal_title：Plant physiology

authors： Facchini PJ,Yu M,Penzes-Yost C

更新日期：1999-07-01 00:00:00

abstract：:The assay of acetyl-CoA carboxylase (EC 6.4.1.2) does not follow ideal zero-order kinetics when assayed in a crude extract from wheat (Triticum aestivum L.) germ. Our results show that the lack of ideality is the consequence of contamination by ATPase and adenylate kinase. These enzyme activities generate significant ...

journal_title：Plant physiology

authors： Eastwell KC,Stumpf PK

更新日期：1983-05-01 00:00:00

abstract：:The gene for the large subunit of Rubisco was specifically mutated by transforming the chloroplast genome of tobacco (Nicotiana tabacum). Codon 335 was altered to encode valine instead of leucine. The resulting mutant plants could not grow without atmospheric CO2 enrichment. In 0.3% (v/v) CO2, the mutant and wild-type...

journal_title：Plant physiology

authors： Whitney SM,von Caemmerer S,Hudson GS,Andrews TJ

更新日期：1999-10-01 00:00:00

abstract：:Grain size is one of the key determinants of grain yield. Although a number of genes that control grain size in rice (Oryza sativa) have been identified, the overall regulatory networks behind this process remain poorly understood. Here, we report the map-based cloning and functional characterization of the quantitati...

journal_title：Plant physiology

authors： Wang A,Hou Q,Si L,Huang X,Luo J,Lu D,Zhu J,Shangguan Y,Miao J,Xie Y,Wang Y,Zhao Q,Feng Q,Zhou C,Li Y,Fan D,Lu Y,Tian Q,Wang Z,Han B

更新日期：2019-08-01 00:00:00

abstract：:Rhizobium promoters involved in the formation of root nodules on leguminous plants are activated by flavonoids in plant root exudate. A series of Rhizobium strains which all contain the inducible Rhizobium leguminosarum nodA promoter fused to the Escherichia coli lacZ gene, and which differ only in the source of the r...

journal_title：Plant physiology

authors： Zaat SA,Wijffelman CA,Mulders IH,van Brussel AA,Lugtenberg BJ

更新日期：1988-04-01 00:00:00

abstract：:Experiments with horizontal etiolated sunflower (Helianthus annuus L.) seedlings supported centrally such that both apical and basal ends are free to react to geostimulus, revealed that the apical end commences curvature 1 to 2 hours earlier than the basal end. The later curvature in the basal region is a consequence ...

journal_title：Plant physiology

authors： Hart JW,Macdonald IR

更新日期：1984-02-01 00:00:00

abstract：:A barley (Hordeum vulgare L.) mutant, nar1a (formerly Az12), deficient in NADH nitrate reductase activity is, nevertheless, capable of growth with nitrate as the sole nitrogen source. In an attempt to identify the mechanism(s) of nitrate reduction in the mutant, nitrate reductase from nar1a was characterized to determ...

journal_title：Plant physiology

authors： Dailey FA,Warner RL,Somers DA,Kleinhofs A

更新日期：1982-05-01 00:00:00

abstract：:The initial rate of Ca2+ movement across the inner-envelope membrane of pea (Pisum sativum L.) chloroplasts was directly measured by stopped-flow spectrofluorometry using membrane vesicles loaded with the Ca2+-sensitive fluorophore fura-2. Calibration of fura-2 fluorescence was achieved by combining a ratiometric meth...

journal_title：Plant physiology

authors： Roh MH,Shingles R,Cleveland MJ,McCarty RE

更新日期：1998-12-01 00:00:00

abstract：:The plant hormone abscisic acid (ABA) is involved in an array of plant processes, including the regulation of gene expression during adaptive responses to various environmental cues. Apart from its well-established role in abiotic stress adaptation, emerging evidence indicates that ABA is also prominently involved in ...

journal_title：Plant physiology

authors： De Vleesschauwer D,Yang Y,Cruz CV,Höfte M

更新日期：2010-04-01 00:00:00

abstract：:Rubisco catalyzes the fixation of CO2 into organic compounds that are used for plant growth and the production of agricultural products, and specific sugar-phosphate derivatives bind tightly to the active sites of Rubisco, locking the enzyme in a catalytically inactive conformation. 2-carboxy-d-arabinitol-1-phosphate ...

journal_title：Plant physiology

authors： Lobo AKM,Orr DJ,Gutierrez MO,Andralojc PJ,Sparks C,Parry MAJ,Carmo-Silva E

更新日期：2019-10-01 00:00:00

abstract：:Isolated maize (Zea mays var. kelvedon glory) etioplasts have been used to investigate the relationships between the spectral shifts and ultrastructural changes which occur during light-induced chloroplast development. After primary photoconversion, the Shibata shift was observed as a change from 680 to 670 nm in the ...

journal_title：Plant physiology

authors： Horton P,Leech RM

更新日期：1975-02-01 00:00:00

abstract：:The rates of utilization of exogenously supplied (14)C labeled acids by corn roots was compared to the utilization of these acids generated endogenously in the mitochondria from acetate-(3)H. (14)C-labeled citrate, pyruvate, succinate, glutamate or aspartate were supplied with acetate-(3)H in a 15 minute pulse and the...

journal_title：Plant physiology

authors： Steer BT,Beevers H

更新日期：1967-09-01 00:00:00

abstract：:We examined the consistency of the native diploid Nicotiana attenuata (Na)'s herbivore-induced transcriptional changes in the two allotetraploid natives, Nicotiana clevelandii (Nc) and Nicotiana quadrivalvis (Nq), which are thought to be derived from hybridizations with an ancestral Na. An analysis of nuclear-encoded ...

journal_title：Plant physiology

authors： Qu N,Schittko U,Baldwin IT

更新日期：2004-05-01 00:00:00

abstract：:Nitrate reductase of the salt-tolerant alga Dunaliella parva could utilize NADPH as well as NADH as an electron donor. The two pyridine nucleotide-dependent activities could not be separated by either ion exchange chromatography on DEAE-cellulose or gel filtration on Sepharose 4B. The NADPH-dependent activity was not ...

journal_title：Plant physiology

authors： Heimer YM

更新日期：1976-07-01 00:00:00

abstract：:Coupling factor particles are associated with membranes of maize etioplasts (Lockshin et al., 1971. Biochim. Biophys. Acta 226: 366-382). In addition, several, but not all, of the polypeptides found in the photosynthetic lamellae of maize chloroplasts are present in etioplasts.During the photoregulated maturation of c...

journal_title：Plant physiology

authors： Forger JM,Bogorad L

更新日期：1973-11-01 00:00:00

abstract：:Ultraviolet B light (UV-B; 280-320 nm) perception and signaling are well-known phenomena in plants, although no specific UV-B photoreceptors have yet been identified. We previously reported on the root UV-B sensitive1 (rus1) mutants in Arabidopsis (Arabidopsis thaliana), which display a block to development under very...

journal_title：Plant physiology

authors： Leasure CD,Tong H,Yuen G,Hou X,Sun X,He ZH

更新日期：2009-08-01 00:00:00

abstract：:Sucrose synthase (EC 2.4.1.13) of sugarbeet (Beta vulgaris L.) fibrous roots has a distinctly different order of preference for nucleoside diphosphate substrates than that of the taproot enzyme (Silvius, Snyder 1979 Plant Physiol 64: 1070-1073). Separation and quantitation of UDP, ADP, and GDP in root extracts by high...

journal_title：Plant physiology

authors： Silvius JE,Snyder FW,Kremer DF

更新日期：1982-07-01 00:00:00

abstract：:The light-harvesting complex (LHC) of photosystem II is composed of several different pigment-binding apoproteins. We have identified a cDNA clone LHCIIa-1 encoding the 31-kilodalton LHC IIa (CP29, Chl a/b-P1) apoprotein of barley (Hordeum vulgare). Direct protein microsequencing of an internal peptide fragment from t...

journal_title：Plant physiology

authors： Morishige DT,Thornber JP

更新日期：1992-01-01 00:00:00

abstract：:Phosphoenolpyruvate carboxylase purified from leaves of maize (Zea mays, L.) is sensitive to the presence of urea. Exposure to 2.5 m urea for 30 min completely inactivates the enzyme, whereas for a concentration of 1.5 m urea, about 1 h is required. Malate appears to have no effect on inactivation by urea of phosphoen...

journal_title：Plant physiology

authors： Wedding RT,Dole P,Chardot TP,Wu MX

更新日期：1992-11-01 00:00:00

abstract：:Apical segments of roots of Zea mays L. cv. Orla and cv. Anjou show a strong georeaction during 7 hours geostimulation. This is abolished by detipping the segments and restored by replacing the tips upon the apical cut surfaces. After exodiffusion of endogenous indoleacetic acid (IAA) the retipped segments showed a si...

journal_title：Plant physiology

authors： Pilet PE

更新日期：1981-05-01 00:00:00

abstract：:Decomposition of 2-chloroethylphosphonic acid (Ethephon) was studied in dried films at various water vapor pressures (0.6 to 86.9 millimeters Hg, 3.2 to 93.9% relative humidity) and temperatures (20, 30, 40, and 50 C) at pH 6.3 and 7.0. The rate of decomposition could be determined equally well by [(14)C]Ethephon or e...

journal_title：Plant physiology

authors： Klein I,Lavee S,Ben-Tal Y

更新日期：1979-03-01 00:00:00

abstract：:Changes in the major chloroplast pigments of pigment-deficient genotypes of soybeans were studied as a function of leaf age and plant age. The trends of pigment development are plotted as a function of relative leaf age at two periods of plant development. Comparisons are made between aging leaves from different nodes...

journal_title：Plant physiology

authors： Eskins K

更新日期：1981-04-01 00:00:00

abstract：:A monoclonal antibody raised against carboxyl-linked IAA was used in Western blot analysis of storage proteins from kernels of Avena sativa, Pennisetum americanum, Sorghum bicolor, and Zea mays. IAA or an IAA-like molecule is associated with the ethanolsoluble protein fraction of the seed. Western blotting of commerci...

journal_title：Plant physiology

authors： Leverone LA,Stroup TL,Caruso JL

更新日期：1991-08-01 00:00:00

abstract：:Tobacco (Nicotiana tabacum L. cv Xanthi) plants were exposed to toxic levels of zinc (Zn). Zn exposure resulted in toxicity signs in plants, and these damages were partly reduced by a calcium (Ca) supplement. Confocal imaging of intracellular Zn using Zinquin showed that Zn was preferentially accumulated in trichomes....

journal_title：Plant physiology

authors： Sarret G,Harada E,Choi YE,Isaure MP,Geoffroy N,Fakra S,Marcus MA,Birschwilks M,Clemens S,Manceau A

更新日期：2006-07-01 00:00:00

abstract：:Using a genetic approach, we have identified and characterized a novel protein, named Msf1 (Maintenance factor for photosystem I), that is required for the maintenance of specific components of the photosynthetic apparatus in the green alga Chlamydomonas reinhardtii Msf1 belongs to the superfamily of light-harvesting ...

journal_title：Plant physiology

authors： Zhao L,Cheng D,Huang X,Chen M,Dall'Osto L,Xing J,Gao L,Li L,Wang Y,Bassi R,Peng L,Wang Y,Rochaix JD,Huang F

更新日期：2017-08-01 00:00:00

abstract：:To determine whether the detergent-solubilized and purified vacuolar H(+)-ATPase from plants was active in H(+) transport, we reconstituted the purified vacuolar ATPase from oat roots (Avena sativa var Lang). Triton-solubilized ATPase activity was purified by gel filtration and ion exchange chromatography. Incorporati...

journal_title：Plant physiology

authors： Ward JM,Sze H

更新日期：1992-07-01 00:00:00

abstract：:The thermophilic blue-green alga Synechococcus lividus was grown at 55 and 38 C. Arrhenius plots of the transient reduction of cytochrome during actinic illumination with light that excited both pigment systems revealed breaks near 43 and 26 C for cells grown at 55 C. In cells grown at 38 C these breaks occurred near ...

journal_title：Plant physiology

authors： Fork DC

更新日期：1979-03-01 00:00:00

abstract：:Ketose reductase (NAD-dependent polyol dehydrogenase EC 1.1.1.14) activity, which catalyzes the NADH-dependent reduction of fructose to sorbitol (d-glucitol), was detected in developing maize (Zea mays L.) endosperm, purified 104-fold from this tissue, and partially characterized. Product analysis by high performance ...

journal_title：Plant physiology

authors： Doehlert DC

更新日期：1987-07-01 00:00:00