Western blot analysis of cereal grain prolamins using an antibody to carboxyl-linked indoleacetic Acid.

Abstract:

:A monoclonal antibody raised against carboxyl-linked IAA was used in Western blot analysis of storage proteins from kernels of Avena sativa, Pennisetum americanum, Sorghum bicolor, and Zea mays. IAA or an IAA-like molecule is associated with the ethanolsoluble protein fraction of the seed. Western blotting of commercial zein, the major storage protein of maize, along with physicochemical evidence reported by Leverone et al. ([1991] Plant Physiol, 96: 1070-1075) indicated that IAA is linked with this prolamin. Results suggest that an IAA-prolamin association may be widespread throughout the Poaceae.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Leverone LA,Stroup TL,Caruso JL

doi

10.1104/pp.96.4.1076

subject

Has Abstract

pub_date

1991-08-01 00:00:00

pages

1076-8

issue

4

eissn

0032-0889

issn

1532-2548

journal_volume

96

pub_type

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