Amino Acid uptake by pea leaf fragments: specificity, energy sources, and mechanism.

Abstract:

:Amino acid uptake into leaf fragments of Pisum sativum depended on metabolism. Glycine uptake was optimal at 30 C and could be supported by respiration and by photosynthesis. Based on studies with an electron flow cofactor, inhibitors, and uncouplers, the energy source for glycine uptake was apparently ATP.The energy-dependent transport of glycine was mediated by a carrier that had a broad specificity for neutral and positively charged l-amino acids. It readily translocated 15 such l-amino acids into the cells, but had a very low affinity for l-aspartate, l-glutamate, d-amino acids, and alpha-aminoisobutyrate. The Ki for competitive inhibition of glycine uptake by another amino acid was equal to the Km for the uptake of that competing species.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Cheung YN,Nobel PS

doi

10.1104/pp.52.6.633

subject

Has Abstract

pub_date

1973-12-01 00:00:00

pages

633-7

issue

6

eissn

0032-0889

issn

1532-2548

journal_volume

52

pub_type

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