Abstract:
:Some properties of microsomal electron transfer chains, dependent for oxidase activity on addition of NADH or NADPH, duroquinone, and oxygen (L. De Luca et al., 1984, Plant Sci Lett 36: 93-98) are described. Activity is characterized by negatively cooperative kinetics toward reduced pyridine nucleotides, with limiting K(m) of 10 to 50 micromolar at pH 7.0 (increasing at lower pH), as well as toward duroquinone with limiting K(m) of 100 to 400 micromolar regardless of pH. Molecular oxygen is reduced by the enzyme complex with S(0.5) of about 30 micromolar and production of H(2)O and H(2)O(2), without superoxide involvement. The ratio NAD(P)H:O(2) averages 1.35 in the presence of KCN and 1.85 in its absence. The pyridine nucleotide specificity of the dehydrogenases has been investigated by kinetic competition experiments. Some enzyme heterogeneity was established for all preparations. At least two enzymes are detectable in plasma membrane-enriched fractions: a major NAD(P)H dehydrogenase having an acid pH optimum, and an NADPH dehydrogenase active around neutrality. Addition of Triton X-100 strongly enhances the activity over most of the pH scale, but depresses it increasingly at pH values higher than 8.0, to the effect that pH profile shows, under these conditions, a major peak at about pH 5.8 for both NADH and NADPH oxidase. Results with endoplasmic reticulum preparations are similar, except that they suggest the presence of still more activities at and above pH 7. The results are interpreted in terms of different complexes catalyzing electron transfer from NAD(P)H to O(2) without release of intermediates.
journal_name
Plant Physioljournal_title
Plant physiologyauthors
Pupillo P,Valenti V,De Luca L,Hertel Rdoi
10.1104/pp.80.2.384subject
Has Abstractpub_date
1986-02-01 00:00:00pages
384-9issue
2eissn
0032-0889issn
1532-2548journal_volume
80pub_type
杂志文章相关文献
PLANT PHYSIOLOGY文献大全abstract::Premature leaf senescence affects plant growth and yield in rice. NAD plays critical roles in cellular redox reactions and remains at a sufficient level in the cell to prevent cell death. Although numerous factors affecting leaf senescence have been identified, few involving NAD biosynthetic pathways have been describ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.15.01898
更新日期:2016-06-01 00:00:00
abstract::Plants of Pisum sativum L. ;Alsweet' were grown under a controlled environment and exposed to SO(2) and O(3) to determine whether changes in stomatal aperture during exposure were related to subsequent leaf injury. Stomata consistently closed with injurious levels of SO(2) and O(3). Measurements with diffusion poromet...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.67.3.539
更新日期:1981-03-01 00:00:00
abstract::The occurrence of the complete orotic acid pathway for the biosynthesis de novo of pyrimidine nucleotides was demonstrated in the intact cells of roots excised from summer squash (Cucurbita pepo L. cv. Early Prolific Straightneck). Evidence that the biosynthesis of pyrimidine nucleotides proceeds via the orotate pathw...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.64.4.562
更新日期:1979-10-01 00:00:00
abstract::Rhizobitoxine, an inhibitor of methionine biosynthesis in Salmonella typhimurium, inhibited ethylene production about 75% in light-grown sorghum seedlings and in senescent apple tissue. Ethylene production stimulated by indoleacetic acid and kinetin in sorghum was similarly inhibited. With both apple and sorghum, the ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.48.1.1
更新日期:1971-07-01 00:00:00
abstract::Soybean (Glycine max [L.] Merrill, cv. Dare) suspension cultures grown in Gamborg B5 medium became discolored and the cells began aggregating after 1 week in culture, especially in the absence of 2,4-dichlorophenoxyacetic acid (2,4-D). The addition of either soluble polyvinylpyrrolidine (PVP) or bovine serum albumin (...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.63.1.206
更新日期:1979-01-01 00:00:00
abstract::The JASMONATE ZIM DOMAIN (JAZ) proteins function as negative regulators of jasmonic acid signaling in plants. We cloned 12 JAZ genes from native tobacco (Nicotiana attenuata), including nine novel JAZs in tobacco, and examined their expression in plants that had leaves elicited by wounding or simulated herbivory. Most...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.112.193771
更新日期:2012-06-01 00:00:00
abstract::Despite a growing number of reports indicating non-Mendelian inheritance of transgene expression in monocots, no detailed description of the structure and stability of the transgene exists for transformants generated by direct DNA-transfer techniques, making the cause for these observations difficult to determine. In ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.115.2.361
更新日期:1997-10-01 00:00:00
abstract::A ureidoglycolate-degrading activity was analyzed in different organs of chickpea (Cicer arietinum). Activity was detected in all the tissues analyzed, but highest levels of specific activity were found in pods, from which it has been purified and characterized. This is the first ureidoglycolate-degrading activity tha...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.125.2.828
更新日期:2001-02-01 00:00:00
abstract::Kinetic characteristics of phosphoenolpyruvate carboxylase (PEPC) from the epiphytic C(3) or C(4): CAM intermediate plant, Peperomia camptotricha, were investigated. Few day versus night differences in V(max),K(m(PEP))), or malate inhibition were observed, even in extracts from water-stressed plants which characterist...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.91.3.1050
更新日期:1989-11-01 00:00:00
abstract::Precise measurements of the minimal fluorescence yield (F(o)) and maximal fluorescence yield (F(m)) of a dark-adapted sample are prerequisites for the quantification of other fluorescence parameters. The pulse amplitude-modulated chlorophyll fluorometer (PAM 101 Chlorophyll Fluorometer, Heinz Walz, Effeltrich, Germany...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.100.1.367
更新日期:1992-09-01 00:00:00
abstract::Plants, algae, and cyanobacteria have developed mechanisms to decrease the energy arriving at reaction centers to protect themselves from high irradiance. In cyanobacteria, the photoactive Orange Carotenoid Protein (OCP) and the Fluorescence Recovery Protein are essential elements in this mechanism. Absorption of stro...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.15.00843
更新日期:2015-09-01 00:00:00
abstract::A theory is presented to explain the phloem mobility of certain systemic xenobiotics that are not weak acids. It is shown that there is a theoretically optimum permeability that permits optimum circulation through the symplasm and apoplast (including the phloem and xylem) of Solanum tuberosum plants. The optimum perme...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.63.2.367
更新日期:1979-02-01 00:00:00
abstract::Protein dephosphorylation by the serine/threonine protein phosphatase 2A (PP2A) modulates a broad array of cellular functions. PP2A normally acts as a heterotrimeric holoenzyme complex comprising a catalytic subunit bound by regulatory A and B subunits. Characterization of the regulatory A subunit isoforms (ROOTS CURL...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.107.112995
更新日期:2008-02-01 00:00:00
abstract::Rabbit antiserum against beta-amylase isolated from germinating seeds of rice was produced, and its specific cross-reactivity with beta-amylase was confirmed by means of Ouchterlony double immunodiffusion and immunoelectrophoresis procedures. The cellular localization of beta-amylase was studied by indirect fluorescen...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.64.2.337
更新日期:1979-08-01 00:00:00
abstract::Epidemics of obesity and type 2 diabetes drive strong consumer interest in plant-based low-calorie sweeteners. Trilobatin is a sweetener found at high concentrations in the leaves of a range of crabapple (Malus) species, but not in domesticated apple (Malus × domestica) leaves, which contain trilobatin's bitter positi...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.20.00807
更新日期:2020-10-01 00:00:00
abstract::Small GTP-binding proteins regulate diverse processes in eukaryotic cells such as signal transduction, cell proliferation, cytoskeletal organization, and intracellular membrane trafficking. These proteins function as molecular switches that cycle between "active" and "inactive" states, and this cycle is linked to the ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.013052
更新日期:2003-03-01 00:00:00
abstract::A cDNA clone encoding rice (Oryza sativa L.) cytosolic triosephosphate isomerase (TPI), an important glycolytic enzyme, was isolated and characterized. The clone (pRTPI-6) contains an open reading frame of 759 base pairs, encoding a polypeptide chain of 253 amino acid residues (M(r) 27,060). The identity of this clone...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.101.2.683
更新日期:1993-02-01 00:00:00
abstract::This study concerns the selective absorption of K and Rb or of K and Na by intact sugar beet (Beta vulgaris) plants from modified conventional nutrient solutions over an extended period of plant growth. Long term results agreed with those of short term experiments by other investigators using excised root systems and ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.47.5.709
更新日期:1971-05-01 00:00:00
abstract::ADP-glucose was found to be the primary sugar nucleotide used for glycogen synthesis by Synechococcus 6301. ADP-glucose pyrophosphorylase was partially purified 12-fold from this blue-green bacterium. The enzyme was activated 8- to 25-fold by glycerate 3-phosphate. Fructose 6-phosphate, fructose 1,6-bisphosphate, 5'-a...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.58.6.753
更新日期:1976-12-01 00:00:00
abstract::The aim of this work was to investigate starch granule numbers in Arabidopsis (Arabidopsis thaliana) leaves. Lack of quantitative information on the extent of genetic, temporal, developmental, and environmental variation in granule numbers is an important limitation in understanding control of starch degradation and t...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.111.186957
更新日期:2012-02-01 00:00:00
abstract::Petunia (Petunia hybrida) pollen requires flavonols (Fl) to germinate. Adding kaempferol to Fl-deficient pollen causes rapid and synchronous germination and tube outgrowth. We exploited this system to identify genes responsive to Fls and to examine the changes in gene expression that occur during the first 0.5 h of po...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.123.2.699
更新日期:2000-06-01 00:00:00
abstract::Storage compound accumulation during seed development prepares the next generation of plants for survival. Therefore, processes involved in the regulation and synthesis of storage compound accumulation during seed development bear relevance to germination and seedling establishment. The wrinkled1 (wri1) mutant of Arab...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.106.079574
更新日期:2006-06-01 00:00:00
abstract::Auxin induction of the proliferation of Nicotiana tabacum (cv Xanthi) mesophyll protoplasts and of protoplast-derived cells was studied. The growth-promoting properties and cytotoxicities at high concentrations of IAA and naphthaleneacetic acid were strongly affected by cell density. The induction of growth by 2,4-dic...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.75.1.54
更新日期:1984-05-01 00:00:00
abstract::The intracellular distribution of enzymes capable of catalyzing the reactions from phosphoglycolate to glycerate in the bluegreen colored eucaryotic alga Cyanidium caldarium has been studied. After separating the organelles from a crude homogenate on a linear flotation gradient, the enzymes glycolate oxidase and gluta...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.90.3.799
更新日期:1989-07-01 00:00:00
abstract::Acetophenones are phenolic compounds involved in the resistance of white spruce (Picea glauca) against spruce budworm (Choristoneura fumiferiana), a major forest pest in North America. The acetophenones pungenol and piceol commonly accumulate in spruce foliage in the form of the corresponding glycosides, pungenin and ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.17.00611
更新日期:2017-10-01 00:00:00
abstract::Amylase was found in high activity (193 international units per milligram protein) in the tap root of alfalfa (Medicago sativa L. cv. Sonora). The activity was separated by gel filtration chromatography into two fractions with molecular weights of 65,700 (heavy amylase) and 41,700 (light amylase). Activity staining of...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.69.5.1096
更新日期:1982-05-01 00:00:00
abstract::In flowering plants, the two sperm cells are embedded within the cytoplasm of the growing pollen tube and as such are passively transported to the embryo sac, wherein double fertilization occurs upon their release. Understanding the mechanisms and conditions by which male gametes mature and take part in fertilization ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.108.125229
更新日期:2008-10-01 00:00:00
abstract::Wheat (Triticum aestivum L. cv Albis) was grown in open-top chambers in the field and fumigated daily with charcoal-filtered air (0.015 microliters per liter O(3)), nonfiltered air (0.03 microliters per liter O(3)), and air enriched with either 0.07 or 0.10 microliters per liter ozone (seasonal 8 hour/day [9 am-5 pm] ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.88.4.1115
更新日期:1988-12-01 00:00:00
abstract::Gravitropic asymmetry of auxin was observed in hypocotyls of tomato (Lycopersicon esculentum Mill.) soon after horizontal placement: the ratio of apically supplied [3H]IAA collected from the lower sides to that from the upper sides was about 1.4 between 5 and 10 minutes. This was adequately early to account for the ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.89.2.652
更新日期:1989-01-01 00:00:00
abstract::To monitor the expression of T-DNA-tagged plant genes in vivo, a collection of 20,261 transgenic lines of Arabidopsis (Columbia-0) were generated with the promoter trap vector pTluc, which carries a promoterless firefly luc (luciferase) reporter gene linked to the right T-DNA border. By detection of bioluminescence in...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.103.027151
更新日期:2004-01-01 00:00:00