Abstract:
:Plants, algae, and cyanobacteria have developed mechanisms to decrease the energy arriving at reaction centers to protect themselves from high irradiance. In cyanobacteria, the photoactive Orange Carotenoid Protein (OCP) and the Fluorescence Recovery Protein are essential elements in this mechanism. Absorption of strong blue-green light by the OCP induces carotenoid and protein conformational changes converting the orange (inactive) OCP into a red (active) OCP. Only the red orange carotenoid protein (OCP(r)) is able to bind to phycobilisomes, the cyanobacterial antenna, and to quench excess energy. In this work, we have constructed and characterized several OCP mutants and focused on the role of the OCP N-terminal arm in photoactivation and excitation energy dissipation. The N-terminal arm largely stabilizes the closed orange OCP structure by interacting with its C-terminal domain. This avoids photoactivation at low irradiance. In addition, it slows the OCP detachment from phycobilisomes by hindering fluorescence recovery protein interaction with bound OCP(r). This maintains thermal dissipation of excess energy for a longer time. Pro-22, at the beginning of the N-terminal arm, has a key role in the correct positioning of the arm in OCP(r), enabling strong OCP binding to phycobilisomes, but is not essential for photoactivation. Our results also show that the opening of the OCP during photoactivation is caused by the movement of the C-terminal domain with respect to the N-terminal domain and the N-terminal arm.
journal_name
Plant Physioljournal_title
Plant physiologyauthors
Thurotte A,Lopez-Igual R,Wilson A,Comolet L,Bourcier de Carbon C,Xiao F,Kirilovsky Ddoi
10.1104/pp.15.00843subject
Has Abstractpub_date
2015-09-01 00:00:00pages
737-47issue
1eissn
0032-0889issn
1532-2548pii
pp.15.00843journal_volume
169pub_type
杂志文章相关文献
PLANT PHYSIOLOGY文献大全abstract::Yariv phenylglycosides [1,3,5-tri(p-glycosyloxyphenylazo)-2,4,6-trihydroxybenzene] are a group of chemical compounds that selectively bind to arabinogalactan proteins (AGPs), a type of plant proteoglycan. Yariv phenylglycosides are widely used as cytochemical reagents to perturb the molecular functions of AGPs as well...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.112.211722
更新日期:2013-03-01 00:00:00
abstract::Dehydration inhibits petal expansion resulting in abnormal flower opening and results in quality loss during the marketing of cut flowers. We constructed a suppression subtractive hybridization library from rose (Rosa hybrida) flowers containing 3,513 unique expressed sequence tags and analyzed their expression profil...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.112.207720
更新日期:2012-12-01 00:00:00
abstract::An oligomycin concentration that specifically inhibits oxidative phosphorylation was added to isolated barley (Hordeum vulgare L.) leaf protoplasts at various irradiances and carbon dioxide concentrations. At saturating as well as low light intensities, photosynthetic oxygen evolution was decreased as a result of the ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.102.3.947
更新日期:1993-07-01 00:00:00
abstract::In C3 plants large amounts of photorespiratory glycine (Gly) are converted to serine by the tetrahydrofolate (THF)-dependent activities of the Gly decarboxylase complex (GDC) and serine hydroxymethyltransferase (SHMT). Using 13C nuclear magnetic resonance, we monitored the flux of carbon through the GDC/SHMT enzyme sy...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.116.1.137
更新日期:1998-01-01 00:00:00
abstract::CO2 uptake rate, chlorophyll fluorescence, and 830-nm absorbance were measured in wild-type (wt) Nicotiana sylvestris (Speg. et Comes) and starchless mutant NS 458 leaves at different light intensities and CO2 concentrations. Initial slopes of the relationships between CO2 uptake and light and CO2 were similar, but th...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.106.2.679
更新日期:1994-10-01 00:00:00
abstract::Nitrogen fixation in the legume-rhizobium symbiosis is a crucial area of research for more sustainable agriculture. Our knowledge of the plant cascade in response to the perception of bacterial Nod factors has increased in recent years. However, the discovery that Nod factors are not involved in the Aeschynomene-Brady...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.15.01134
更新日期:2015-12-01 00:00:00
abstract::The patterns of nitrate reductase activity (NRA) in the leaves (in vivo assay) and root nodule nitrogenase activity (C(2)H(2) reduction) were investigated throughout the season in field-grown Phaseolus vulgaris plants.Maximal NRA (per g fresh weight) occurred at early stages of leaf development but total activity (per...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.63.3.421
更新日期:1979-03-01 00:00:00
abstract::The changes in root system architecture (RSA) triggered by phosphate (P) deprivation were studied in Arabidopsis (Arabidopsis thaliana) plants grown for 14 d on 1 mM or 3 microM P. Two different temporal phases were observed in the response of RSA to low P. First, lateral root (LR) development was promoted between day...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.105.060061
更新日期:2005-08-01 00:00:00
abstract::A starch deficient mutant of Arabidopsis thaliana (L.) Heynh. has been isolated in which leaf extracts contain only about 5% as much activity of ADPglucose pyrophosphorylase (EC 2.7.7.27) as the wild type. A single, nuclear mutation at a previously undescribed locus designated adg2 is responsible for the mutant phenot...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.88.4.1175
更新日期:1988-12-01 00:00:00
abstract::An important question concerning the role of carboxyarabinitol 1-phosphate (CA1P) metabolism in the light-dependent regulation of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) activity is the extent to which CA1P is bound to Rubisco in vivo. We report here the development of an extraction procedure using a...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.105.2.731
更新日期:1994-06-01 00:00:00
abstract::Four cDNAs, one encoding an alpha-subunit and three encoding beta-subunits of the mitochondrial pyruvate dehydrogenase, were isolated from maize (Zea mays L.) libraries. The deduced amino acid sequences of both alpha- and beta-subunits are approximately 80% identical with Arabidopsis and pea (Pisum sativum L.) homolog...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.119.2.635
更新日期:1999-02-01 00:00:00
abstract::Plant cystatins, similar to other defense proteins, include hypervariable, positively selected amino acid sites presumably impacting their biological activity. Using 29 single mutants of the eighth domain of tomato (Solanum lycopersicum) multicystatin, SlCYS8, we assessed here the potential of site-directed mutagenesi...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.108.115741
更新日期:2008-03-01 00:00:00
abstract::Purified glycopeptides derived from yeast invertase act as highly potent elicitors in suspension-cultured tomato (Lycopersicon esculentum [L.] Mill) cells, inducing ethylene biosynthesis and phenylalanine ammonia lyase half-maximally at concentrations of 1 to 5 nM. We previously demonstrated the presence of a high-aff...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.112.4.1659
更新日期:1996-12-01 00:00:00
abstract::The stimulation of the Hill reaction in CO(2)-depleted broken chloroplasts (Pisum sativum L. cv Rondo) by the total amount of dissolved CO(2) and HCO(3) (-) (bicarbonate(*)) was measured at several formate concentrations. Formate appears to be a competitive inhibitor of the bicarbonate(*) stimulation of electron flow....
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.75.1.146
更新日期:1984-05-01 00:00:00
abstract::Two inhibitory fractions (B(1) and C) from extracts of immature fruit of carob were tested for their ability to inhibit the action of indoleacetic acid (IAA) in three bioassays. There was no reduction of IAA-induced reactions in the Avena curvature test, abscission of debladed coleus petioles, or growth of cucumber hy...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.46.4.531
更新日期:1970-10-01 00:00:00
abstract::Chloroplast ribosomes, which originated from cyanobacteria, comprise a large subunit (50S) and a small subunit (30S) containing ribosomal RNAs (rRNAs) and various ribosomal proteins. Genes for many chloroplast ribosomal proteins, as well as proteins with auxiliary roles in ribosome biogenesis or functioning, reside in...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.18.00602
更新日期:2018-08-01 00:00:00
abstract::An alpha-L-fucosidase (EC 3.2.1.51) able to release the t-fucosyl residue from the side chain of xyloglucan oligosaccharides has been detected in the leaves of Arabidopsis plants. Moreover, an alpha-L-fucosidase with similar substrate specificity was purified from cabbage (Brassica oleracea) leaves to render a single ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:
更新日期:2002-01-01 00:00:00
abstract::Increased levels of solar ultraviolet (290-320 nm) (UV-B) radiation could have profound effects on plant proteins because the aromatic amino acids in proteins absorb strongly in this spectral region. We have investigated the effects of UV-B radiation on plant proteins and have observed a novel 66-kD protein. This prod...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.109.1.221
更新日期:1995-09-01 00:00:00
abstract::Phytoene synthase (PSY) catalyzes the highly regulated, frequently rate-limiting synthesis of the first biosynthetically formed carotene. While PSY constitutes a small gene family in most plant taxa, the Brassicaceae, including Arabidopsis (Arabidopsis thaliana), predominantly possess a single PSY gene. This monogenic...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.16.01262
更新日期:2016-12-01 00:00:00
abstract::Relationships between net plant CO(2) exchange rate (CER) and canopy development were examined in ;jubilee' tomato over the initial 4 weeks of vegetative growth. A comparison was made between two plant groups that were alternatively exposed to 200 or 800 microeinsteins per square meter per second midday irradiation to...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.80.3.711
更新日期:1986-03-01 00:00:00
abstract::Sex determination in cucumber (Cucumis sativus L.) is controlled largely by three genes: F, m, and a. The F and m loci interact to produce monoecious (M_f_) or gynoecious (M_f_) sex phenotypes. Ethylene and factors that induce ethylene biosynthesis, such as 1-aminocyclopropane-1-carboxylate (ACC) and auxin, also enhan...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.113.3.987
更新日期:1997-03-01 00:00:00
abstract::Optimization of nitrogen fixation by rhizobia in legumes is a key area of research for sustainable agriculture. Symbiotic nitrogen fixation (SNF) occurs in specialized organs called nodules and depends on a steady supply of carbon to both plant and bacterial cells. Here we report the functional characterization of a n...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.15.01910
更新日期:2016-05-01 00:00:00
abstract::A monoclonal antibody raised against carboxyl-linked IAA was used in Western blot analysis of storage proteins from kernels of Avena sativa, Pennisetum americanum, Sorghum bicolor, and Zea mays. IAA or an IAA-like molecule is associated with the ethanolsoluble protein fraction of the seed. Western blotting of commerci...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.96.4.1076
更新日期:1991-08-01 00:00:00
abstract::Extracts prepared from floral meristematic tissue of alfalfa (Medicago sativa L.) were investigated for expression of the enzyme transglutaminase in order to identify the major protein substrate for transglutaminase-directed modifications among plant proteins. The large polymorphic subunits of ribulose 1,5-bisphosphat...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.92.1.88
更新日期:1990-01-01 00:00:00
abstract::Indole-3-acetic acid was applied asymmetrically to the hypocotyls of sunflower (Helianthus annuus L.) seedlings. After 5 hours on a clinostat, auxin gradients as small as 1 to 1.3 produced substantial (more than 60 degrees) hypocotyl curvature. This result suggests the asymmetric growth underlying hypocotyl gravitro...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.91.2.466
更新日期:1989-01-01 00:00:00
abstract::Soybean (Glycine max L. Merr.) germplasm, isogenic except for loci controlling male sterility (ms(1)), was utilized to study the effects of reproductive development on certain aspects of photosynthesis. Plants were sampled at various times between flowering (77 days after transplanting) and maturity (147 days after tr...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.84.4.1357
更新日期:1987-08-01 00:00:00
abstract::The capacity of sodium orthovanadate to inhibit the plasmalemma H(+) ATPase of Nitella translucens internodal cells in vivo was tested. Here we show that 1 millimolar vanadate added externally depolarizes strongly and permanently the membrane potential, both in dark and light, to the Nernst potential for potassium con...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.96.1.91
更新日期:1991-05-01 00:00:00
abstract::Enhancing nitrogen use efficiency (NUE) in crop plants is an important breeding target to reduce excessive use of chemical fertilizers, with substantial benefits to farmers and the environment. In Arabidopsis (Arabidopsis thaliana), allocation of more NO3 (-) to shoots was associated with higher NUE; however, the comm...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.15.01377
更新日期:2016-03-01 00:00:00
abstract::The fifth increased branching ramosus (rms) mutant, rms5, from pea (Pisum sativum), is described here for phenotype and grafting responses with four other rms mutants. Xylem sap zeatin riboside concentration and shoot auxin levels in rms5 plants have also been compared with rms1 and wild type (WT). Rms1 and Rms5 appea...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.126.3.1205
更新日期:2001-07-01 00:00:00
abstract::The mechanism of sucrose transport was investigated in plasma membrane (PM) vesicles isolated from spinach (Spinacia oleracea L.) leaves. PM vesicles were isolated by aqueous two-phase partitioning and were equilibrated in pH 7.8 buffer containing K(+). The vesicles rapidly accumulated sucrose in the presence of a tra...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.96.2.615
更新日期:1991-06-01 00:00:00