Abstract:
:Enhancing nitrogen use efficiency (NUE) in crop plants is an important breeding target to reduce excessive use of chemical fertilizers, with substantial benefits to farmers and the environment. In Arabidopsis (Arabidopsis thaliana), allocation of more NO3 (-) to shoots was associated with higher NUE; however, the commonality of this process across plant species have not been sufficiently studied. Two Brassica napus genotypes were identified with high and low NUE. We found that activities of V-ATPase and V-PPase, the two tonoplast proton-pumps, were significantly lower in roots of the high-NUE genotype (Xiangyou15) than in the low-NUE genotype (814); and consequently, less vacuolar NO3 (-) was retained in roots of Xiangyou15. Moreover, NO3 (-) concentration in xylem sap, [(15)N] shoot:root (S:R) and [NO3 (-)] S:R ratios were significantly higher in Xiangyou15. BnNRT1.5 expression was higher in roots of Xiangyou15 compared with 814, while BnNRT1.8 expression was lower. In both B. napus treated with proton pump inhibitors or Arabidopsis mutants impaired in proton pump activity, vacuolar sequestration capacity (VSC) of NO3 (-) in roots substantially decreased. Expression of NRT1.5 was up-regulated, but NRT1.8 was down-regulated, driving greater NO3 (-) long-distance transport from roots to shoots. NUE in Arabidopsis mutants impaired in proton pumps was also significantly higher than in the wild type col-0. Taken together, these data suggest that decrease in VSC of NO3 (-) in roots will enhance transport to shoot and essentially contribute to higher NUE by promoting NO3 (-) allocation to aerial parts, likely through coordinated regulation of NRT1.5 and NRT1.8.
journal_name
Plant Physioljournal_title
Plant physiologyauthors
Han YL,Song HX,Liao Q,Yu Y,Jian SF,Lepo JE,Liu Q,Rong XM,Tian C,Zeng J,Guan CY,Ismail AM,Zhang ZHdoi
10.1104/pp.15.01377subject
Has Abstractpub_date
2016-03-01 00:00:00pages
1684-98issue
3eissn
0032-0889issn
1532-2548pii
pp.15.01377journal_volume
170pub_type
杂志文章相关文献
PLANT PHYSIOLOGY文献大全abstract::In pea leaves, much of the metabolism of imported asparagine is by transamination. This activity was previously shown to be localized in the peroxisomes, suggesting a possible connection between asparagine and photorespiratory nitrogen metabolism. This was investigated by examination of the transfer of (15)N from the ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.78.2.334
更新日期:1985-06-01 00:00:00
abstract::Salinity-induced alterations in tomato (Lypersicon esculentum Mill. cv Heinz 1350) root plasma membrane properties were studied and characterized using a membrane vesicle system. Equivalent rates of MgATP-dependent H(+)-transport activity were measured by quinacrine fluorescence (DeltapH) in plasma membrane vesicles i...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.93.2.471
更新日期:1990-06-01 00:00:00
abstract::In plants, bZIP (basic leucine zipper) transcription factors regulate diverse processes such as development and stress responses. However, few of these transcription factors have been functionally characterized in maize (Zea mays). In this study, we characterized the bZIP transcription factor gene ZmbZIP4 from maize. ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.18.00436
更新日期:2018-10-01 00:00:00
abstract::The nature of the association between nitrate reductase (NR) and membranes was examined. Nitrate reductase activity (NRA) associated with the microsomal fraction of barley (Hordeum vulgare L.) roots amounted to 0.6 to 0.8% of soluble NRA following sonication in the presence of 250 mM KI and repeated osmotic shock. T...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.104.3.925
更新日期:1994-01-01 00:00:00
abstract::The effect of external CO2 concentration on the expression of carbonic anhydrase (CA) and ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) was examined in pea (Pisum sativum cv Little Marvel) leaves. Enzyme activities and their transcript levels were reduced in plants grown at 1000 [mu]L/L CO2 compared with p...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.112.2.569
更新日期:1996-10-01 00:00:00
abstract::ADP-glucose pyrophosphorylase was partially purified from Chlorella vulgaris 11h. 3-Phosphoglycerate activated the enzyme by lowering the Michaelis constant for glucose-1-phosphate (from 0.97 to 0.36 millimolar in the presence of 2 millimolar phosphoglycerate) and ATP (from 0.23 to 0.10 millimolar), as well as increas...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.78.3.601
更新日期:1985-07-01 00:00:00
abstract::The cDNA for 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase of Arabidopsis encodes a polypeptide with an amino-terminal signal sequence for plastid import. A cDNA fragment encoding the processed form of the enzyme was expressed in Escherichia coli. The resulting protein was purified to electrophoretic homogeneit...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.002626
更新日期:2002-08-01 00:00:00
abstract::The wilty tomato mutant, flacca, and the control variety, Rheinlands Ruhm, were compared with regard to the endogenous activity and concentration of auxin- and abscisic acid-like substances during ontogeny. The mutant wilts fast under water deficit because of inability to close its stomata. Symptoms characteristic of ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.46.3.373
更新日期:1970-09-01 00:00:00
abstract::Influx and accumulation of K(+) in barley (Hordeum vulgare L. cv Fergus) roots were measured at two temperatures (10 degrees C and 20 degrees C) in plants which had been grown with roots and shoots at 20 degrees C (HT plants), with roots and shoots at 10 degrees C (LT plants), and with roots at 10 degrees C and shoots...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.74.3.730
更新日期:1984-03-01 00:00:00
abstract::We report the molecular cloning and sequence of the cDNA coding for the biotin-containing subunit of the chloroplastic acetylcoenzyme A (CoA) carboxylase (ACCase) of Arabidopsis thaliana (CAC1). The 3' end of the CAC1 sequence, coding for a peptide of 94 amino acids, which includes a putative biotinylation motif, was ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.109.2.619
更新日期:1995-10-01 00:00:00
abstract::Amyloplast envelope membranes isolated from cultured, white-wild cells of sycamore (Acer pseudoplatanus L.) have been found to contain a Mg(2+)-ATPase, ranging in specific activity from 5 to 30 nanomoles per minute per milligram protein. This ATPase hydrolyzes a broad range of nucleoside triphosphates, whereas it hydr...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.88.1.119
更新日期:1988-09-01 00:00:00
abstract::Hydrogen peroxide (H2O2) is a stable component of reactive oxygen species, and its production in plants represents the successful recognition of pathogen infection and pathogen-associated molecular patterns (PAMPs). This production of H2O2 is typically apoplastic but is subsequently associated with intracellular immun...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.15.01237
更新日期:2016-07-01 00:00:00
abstract::Western blots of soluble protein from wheat, rice, and corn showed that ADPglucose pyrophosphorylase subunits have a size of 50 kilodaltons from endosperm tissue and 43 and 46 kilodaltons from leaf. Antisera to ADPglucose pyrophosphorylase precipitated in vitro translation products of 73 and 76 kilodaltons when leaf p...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.81.2.642
更新日期:1986-06-01 00:00:00
abstract::Potato (Solanum tuberosum L. cv Saturna) plants were grown in pots with varying supplies of K fertilizer (1.25, 5, and 10 grams K(2)O per 12 kilograms soil). Four weeks after midflower, plants were supplied with (14)CO(2) for 12 hours and osmotic and water potential in tubers were determined. Assimilation of (14)CO(2)...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.73.4.956
更新日期:1983-12-01 00:00:00
abstract::The primary storage protein of oat (Avena sativa L.) seeds, globulin, was shown to have a specific carbohydrate-binding activity. The globulin was capable of hemagglutinating rabbit red blood cells and this hemagglutination was inhibited by the beta-glucan, laminarin, as well as by carbohydrate which had been cleaved ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.74.3.675
更新日期:1984-03-01 00:00:00
abstract::Electrophysiological studies in roots of pea (Pisum sativum L.) and rigid ryegrass (Lolium rigidum Gaud.) seedlings were conducted to elucidate the mechanism involved in the membrane response to the herbicide diclofop. In pea, a dicotyledonous plant insensitive to diclofop, membrane depolarization at varying pH values...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.102.4.1331
更新日期:1993-08-01 00:00:00
abstract::Growth of the aerial part of the plant is dependent upon the maintenance of the shoot apical meristem (SAM). A balance between the self-renewing stem cells in the central zone (CZ) and organogenesis in the peripheral zone (PZ) is essential for the integrity, function, and maintenance of the SAM. Understanding how the ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.110.171462
更新日期:2011-06-01 00:00:00
abstract::Aldolase activity of Chara foetida (Braun) could be separated into a minor (peak I) and a major peak (peak II) by ion-exchange chromatography on DEAE-cellulose. Affinity chromatography on P-cellulose resulted in highly purified aldolase preparations with specific activities of 3.2 and 4.8 units per milligram protein a...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.87.1.78
更新日期:1988-05-01 00:00:00
abstract::The occurrence of fungicidal, elemental S is well documented in certain specialized prokaryotes, but has rarely been detected in eukaryotes. Elemental S was first identified in this laboratory as a novel phytoalexin in the xylem of resistant genotypes of Theobroma cacao, after infection by the vascular, fungal pathoge...
journal_title:Plant physiology
pub_type: 杂志文章
doi:
更新日期:2002-01-01 00:00:00
abstract::Plant hormones modulate plant growth, development, and defense. However, many aspects of the origin and evolution of plant hormone signaling pathways remain obscure. Here, we use a comparative genomic and phylogenetic approach to investigate the origin and evolution of nine major plant hormone (abscisic acid, auxin, b...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.114.247403
更新日期:2015-03-01 00:00:00
abstract::The plant cuticle is an extracellular hydrophobic layer that covers the aerial epidermis of all land plants, providing protection against desiccation and external environmental stresses. The past decade has seen considerable progress in assembling models for the biosynthesis of its two major components, the polymer cu...
journal_title:Plant physiology
pub_type: 杂志文章,评审
doi:10.1104/pp.113.222737
更新日期:2013-09-01 00:00:00
abstract::Soybean (Glycine max [L.] Merr.) seedlings grown in the absence of combined N and in an Ar:O(2) (79:21, volume/volume) atmosphere had greater seedling and nodule mass, threefold higher acetylene reducing activity per gram fresh weight nodules, no observable increase in nitrogenase Fe-protein, and a higher energy charg...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.96.1.305
更新日期:1991-05-01 00:00:00
abstract::Transgenic Nicotiana tabacum (cv Petit Havana SR1) containing high levels of mitochondrial alternative oxidase (AOX) protein due to the introduction of a sense transgene(s) of Aox1, the nuclear gene encoding AOX, were used to investigate mechanisms regulating AOX activity. After purification of leaf mitochondria, a la...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.109.2.353
更新日期:1995-10-01 00:00:00
abstract::A technical procedure is described to follow the in vitro release of the ;male germ unit' and the sperm cells from three tricellular pollen species (Brassica, Zea, and Triticum). The condition of the sperm cell was controlled using light microscopy. In addition, for the first time, the sperm cells viability has been c...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.83.3.464
更新日期:1987-03-01 00:00:00
abstract::Leaves of woad (Isatis tinctoria L.) were found to incorporate efficiently tritiated indoleacetaldoxime and (35)S from (35)S-l-cystine into glucobrassicin and sulfoglucobrassicin. Time course of incorporation of (35)S from (35)S-cystine into the glucosinolates indicated that glucobrassicin was formed first and then su...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.50.1.43
更新日期:1972-07-01 00:00:00
abstract::Recently, we isolated dehydroipomeamarone, a new sesquiterpenoid from sweet potato (Ipomoea batatas Lam.) root tissue infected with Ceratocystis fimbriata (Ell. et Halst.). The purpose of this investigation was to determine whether dehydroipomeamarone was a precursor in the biosynthetic pathway of ipomeamarone. The in...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.53.4.649
更新日期:1974-04-01 00:00:00
abstract::Mitochondrial citrate synthase was purified from leaves of Pisum sativum L. cv Progress 9. A three step purification was employed using ATP-Sepharose affinity chromatography which resulted in a 600-fold enrichment. Enzyme activity was assayed spectrophotometrically during greening of etiolated leaves under constant wh...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.89.3.719
更新日期:1989-03-01 00:00:00
abstract::Cotyledon explants from germinated 1-day-old soybean seedling were inoculated with single or mixed strains of Agrobacterium tumefaciens. Mixed-strain infections with the supervirulent L,L-succinamopine type strain A281 (pTiBo542) and strain LBA4404 carrying an octopine type virulence (vir) region and a binary vector (...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.88.3.570
更新日期:1988-11-01 00:00:00
abstract::Absorption of selenate and selenite by excised roots of Astragalus Crotalariae, a selenium accumulator, and of A. lentiginosus, a non-accumulator, was favored by CaCl(2) and a pH of 4.0. The uptake of selenate and possibly selenite, is metabolically linked. Roots of a number of Astragalus species were examined, and in...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.43.1.14
更新日期:1968-01-01 00:00:00
abstract::Sucrose synthase (Sus; EC 2.4.1.13) is a key enzyme of sucrose metabolism in plant cells, providing carbon for respiration and for the synthesis of cell wall polymers and starch. Since Sus is important for plant cell growth, insights into its structure, localization, and features are useful for defining the relationsh...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.108.115956
更新日期:2008-08-01 00:00:00