Nitrogen Use Efficiency Is Mediated by Vacuolar Nitrate Sequestration Capacity in Roots of Brassica napus.

Abstract:

:Enhancing nitrogen use efficiency (NUE) in crop plants is an important breeding target to reduce excessive use of chemical fertilizers, with substantial benefits to farmers and the environment. In Arabidopsis (Arabidopsis thaliana), allocation of more NO3 (-) to shoots was associated with higher NUE; however, the commonality of this process across plant species have not been sufficiently studied. Two Brassica napus genotypes were identified with high and low NUE. We found that activities of V-ATPase and V-PPase, the two tonoplast proton-pumps, were significantly lower in roots of the high-NUE genotype (Xiangyou15) than in the low-NUE genotype (814); and consequently, less vacuolar NO3 (-) was retained in roots of Xiangyou15. Moreover, NO3 (-) concentration in xylem sap, [(15)N] shoot:root (S:R) and [NO3 (-)] S:R ratios were significantly higher in Xiangyou15. BnNRT1.5 expression was higher in roots of Xiangyou15 compared with 814, while BnNRT1.8 expression was lower. In both B. napus treated with proton pump inhibitors or Arabidopsis mutants impaired in proton pump activity, vacuolar sequestration capacity (VSC) of NO3 (-) in roots substantially decreased. Expression of NRT1.5 was up-regulated, but NRT1.8 was down-regulated, driving greater NO3 (-) long-distance transport from roots to shoots. NUE in Arabidopsis mutants impaired in proton pumps was also significantly higher than in the wild type col-0. Taken together, these data suggest that decrease in VSC of NO3 (-) in roots will enhance transport to shoot and essentially contribute to higher NUE by promoting NO3 (-) allocation to aerial parts, likely through coordinated regulation of NRT1.5 and NRT1.8.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Han YL,Song HX,Liao Q,Yu Y,Jian SF,Lepo JE,Liu Q,Rong XM,Tian C,Zeng J,Guan CY,Ismail AM,Zhang ZH

doi

10.1104/pp.15.01377

subject

Has Abstract

pub_date

2016-03-01 00:00:00

pages

1684-98

issue

3

eissn

0032-0889

issn

1532-2548

pii

pp.15.01377

journal_volume

170

pub_type

杂志文章
  • Role of asparagine in the photorespiratory nitrogen metabolism of pea leaves.

    abstract::In pea leaves, much of the metabolism of imported asparagine is by transamination. This activity was previously shown to be localized in the peroxisomes, suggesting a possible connection between asparagine and photorespiratory nitrogen metabolism. This was investigated by examination of the transfer of (15)N from the ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.78.2.334

    authors: Ta TC,Joy KW,Ireland RJ

    更新日期:1985-06-01 00:00:00

  • Electrostatic Changes in Lycopersicon esculentum Root Plasma Membrane Resulting from Salt Stress.

    abstract::Salinity-induced alterations in tomato (Lypersicon esculentum Mill. cv Heinz 1350) root plasma membrane properties were studied and characterized using a membrane vesicle system. Equivalent rates of MgATP-dependent H(+)-transport activity were measured by quinacrine fluorescence (DeltapH) in plasma membrane vesicles i...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.93.2.471

    authors: Suhayda CG,Giannini JL,Briskin DP,Shannon MC

    更新日期:1990-06-01 00:00:00

  • ZmbZIP4 Contributes to Stress Resistance in Maize by Regulating ABA Synthesis and Root Development.

    abstract::In plants, bZIP (basic leucine zipper) transcription factors regulate diverse processes such as development and stress responses. However, few of these transcription factors have been functionally characterized in maize (Zea mays). In this study, we characterized the bZIP transcription factor gene ZmbZIP4 from maize. ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.18.00436

    authors: Ma H,Liu C,Li Z,Ran Q,Xie G,Wang B,Fang S,Chu J,Zhang J

    更新日期:2018-10-01 00:00:00

  • Characterization of the association of nitrate reductase with barley (Hordeum vulgare L.) root membranes.

    abstract::The nature of the association between nitrate reductase (NR) and membranes was examined. Nitrate reductase activity (NRA) associated with the microsomal fraction of barley (Hordeum vulgare L.) roots amounted to 0.6 to 0.8% of soluble NRA following sonication in the presence of 250 mM KI and repeated osmotic shock. T...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.104.3.925

    authors: Meyerhoff PA,Fox TC,Travis RL,Huffaker RC

    更新日期:1994-01-01 00:00:00

  • Effect of CO2 Concentration on Carbonic Anhydrase and Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase Expression in Pea.

    abstract::The effect of external CO2 concentration on the expression of carbonic anhydrase (CA) and ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) was examined in pea (Pisum sativum cv Little Marvel) leaves. Enzyme activities and their transcript levels were reduced in plants grown at 1000 [mu]L/L CO2 compared with p...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.112.2.569

    authors: Majeau N,Coleman JR

    更新日期:1996-10-01 00:00:00

  • Regulation of ADP-Glucose Pyrophosphorylase from Chlorella vulgaris.

    abstract::ADP-glucose pyrophosphorylase was partially purified from Chlorella vulgaris 11h. 3-Phosphoglycerate activated the enzyme by lowering the Michaelis constant for glucose-1-phosphate (from 0.97 to 0.36 millimolar in the presence of 2 millimolar phosphoglycerate) and ATP (from 0.23 to 0.10 millimolar), as well as increas...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.78.3.601

    authors: Nakamura Y,Imamura M

    更新日期:1985-07-01 00:00:00

  • Redox regulation of Arabidopsis 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase.

    abstract::The cDNA for 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase of Arabidopsis encodes a polypeptide with an amino-terminal signal sequence for plastid import. A cDNA fragment encoding the processed form of the enzyme was expressed in Escherichia coli. The resulting protein was purified to electrophoretic homogeneit...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.002626

    authors: Entus R,Poling M,Herrmann KM

    更新日期:2002-08-01 00:00:00

  • Abnormal Stomatal Behavior and Hormonal Imbalance in flacca, a Wilty Mutant of Tomato: II. Auxin- and Abscisic Acid-like Activity.

    abstract::The wilty tomato mutant, flacca, and the control variety, Rheinlands Ruhm, were compared with regard to the endogenous activity and concentration of auxin- and abscisic acid-like substances during ontogeny. The mutant wilts fast under water deficit because of inability to close its stomata. Symptoms characteristic of ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.46.3.373

    authors: Tal M,Imber D

    更新日期:1970-09-01 00:00:00

  • Regulation of k influx in barley : effects of low temperature.

    abstract::Influx and accumulation of K(+) in barley (Hordeum vulgare L. cv Fergus) roots were measured at two temperatures (10 degrees C and 20 degrees C) in plants which had been grown with roots and shoots at 20 degrees C (HT plants), with roots and shoots at 10 degrees C (LT plants), and with roots at 10 degrees C and shoots...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.74.3.730

    authors: Siddiqi MY,Memon AR,Glass AD

    更新日期:1984-03-01 00:00:00

  • Molecular cloning and characterization of the cDNA coding for the biotin-containing subunit of the chloroplastic acetyl-coenzyme A carboxylase.

    abstract::We report the molecular cloning and sequence of the cDNA coding for the biotin-containing subunit of the chloroplastic acetylcoenzyme A (CoA) carboxylase (ACCase) of Arabidopsis thaliana (CAC1). The 3' end of the CAC1 sequence, coding for a peptide of 94 amino acids, which includes a putative biotinylation motif, was ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.109.2.619

    authors: Choi JK,Yu F,Wurtele ES,Nikolau BJ

    更新日期:1995-10-01 00:00:00

  • Characterization of an ATPase Associated with the Inner Envelope Membrane of Amyloplasts from Suspension-Cultured Cells of Sycamore (Acer pseudoplatanus L.).

    abstract::Amyloplast envelope membranes isolated from cultured, white-wild cells of sycamore (Acer pseudoplatanus L.) have been found to contain a Mg(2+)-ATPase, ranging in specific activity from 5 to 30 nanomoles per minute per milligram protein. This ATPase hydrolyzes a broad range of nucleoside triphosphates, whereas it hydr...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.88.1.119

    authors: Harinasut P,Takabe T,Akazawa T,Tagaya M,Fukui T

    更新日期:1988-09-01 00:00:00

  • Plant Aquaporin AtPIP1;4 Links Apoplastic H2O2 Induction to Disease Immunity Pathways.

    abstract::Hydrogen peroxide (H2O2) is a stable component of reactive oxygen species, and its production in plants represents the successful recognition of pathogen infection and pathogen-associated molecular patterns (PAMPs). This production of H2O2 is typically apoplastic but is subsequently associated with intracellular immun...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.15.01237

    authors: Tian S,Wang X,Li P,Wang H,Ji H,Xie J,Qiu Q,Shen D,Dong H

    更新日期:2016-07-01 00:00:00

  • ADPglucose Pyrophosphorylase Is Encoded by Different mRNA Transcripts in Leaf and Endosperm of Cereals.

    abstract::Western blots of soluble protein from wheat, rice, and corn showed that ADPglucose pyrophosphorylase subunits have a size of 50 kilodaltons from endosperm tissue and 43 and 46 kilodaltons from leaf. Antisera to ADPglucose pyrophosphorylase precipitated in vitro translation products of 73 and 76 kilodaltons when leaf p...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.81.2.642

    authors: Krishnan HB,Reeves CD,Okita TW

    更新日期:1986-06-01 00:00:00

  • Water relationships and incorporation of C assimilates in tubers of potato plants differing in potassium nutrition.

    abstract::Potato (Solanum tuberosum L. cv Saturna) plants were grown in pots with varying supplies of K fertilizer (1.25, 5, and 10 grams K(2)O per 12 kilograms soil). Four weeks after midflower, plants were supplied with (14)CO(2) for 12 hours and osmotic and water potential in tubers were determined. Assimilation of (14)CO(2)...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.73.4.956

    authors: Beringer H,Haeder HE,Lindhauer M

    更新日期:1983-12-01 00:00:00

  • A Seed Storage Protein with Possible Self-Affinity through Lectin-Like Binding.

    abstract::The primary storage protein of oat (Avena sativa L.) seeds, globulin, was shown to have a specific carbohydrate-binding activity. The globulin was capable of hemagglutinating rabbit red blood cells and this hemagglutination was inhibited by the beta-glucan, laminarin, as well as by carbohydrate which had been cleaved ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.74.3.675

    authors: Langston-Unkefer PJ,Gade W

    更新日期:1984-03-01 00:00:00

  • Membrane Response to Diclofop Acid Is pH Dependent and Is Regulated by the Protonated Form of the Herbicide in Roots of Pea and Resistant and Susceptible Rigid Ryegrass.

    abstract::Electrophysiological studies in roots of pea (Pisum sativum L.) and rigid ryegrass (Lolium rigidum Gaud.) seedlings were conducted to elucidate the mechanism involved in the membrane response to the herbicide diclofop. In pea, a dicotyledonous plant insensitive to diclofop, membrane depolarization at varying pH values...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.102.4.1331

    authors: DiTomaso JM

    更新日期:1993-08-01 00:00:00

  • The role of PENNYWISE and POUND-FOOLISH in the maintenance of the shoot apical meristem in Arabidopsis.

    abstract::Growth of the aerial part of the plant is dependent upon the maintenance of the shoot apical meristem (SAM). A balance between the self-renewing stem cells in the central zone (CZ) and organogenesis in the peripheral zone (PZ) is essential for the integrity, function, and maintenance of the SAM. Understanding how the ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.110.171462

    authors: Ung N,Lal S,Smith HM

    更新日期:2011-06-01 00:00:00

  • Two Class I Aldolases in the Green Alga Chara foetida (Charophyceae).

    abstract::Aldolase activity of Chara foetida (Braun) could be separated into a minor (peak I) and a major peak (peak II) by ion-exchange chromatography on DEAE-cellulose. Affinity chromatography on P-cellulose resulted in highly purified aldolase preparations with specific activities of 3.2 and 4.8 units per milligram protein a...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.87.1.78

    authors: Jacobshagen S,Schnarrenberger C

    更新日期:1988-05-01 00:00:00

  • Elemental sulfur and thiol accumulation in tomato and defense against a fungal vascular pathogen.

    abstract::The occurrence of fungicidal, elemental S is well documented in certain specialized prokaryotes, but has rarely been detected in eukaryotes. Elemental S was first identified in this laboratory as a novel phytoalexin in the xylem of resistant genotypes of Theobroma cacao, after infection by the vascular, fungal pathoge...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:

    authors: Williams JS,Hall SA,Hawkesford MJ,Beale MH,Cooper RM

    更新日期:2002-01-01 00:00:00

  • Insights into the origin and evolution of the plant hormone signaling machinery.

    abstract::Plant hormones modulate plant growth, development, and defense. However, many aspects of the origin and evolution of plant hormone signaling pathways remain obscure. Here, we use a comparative genomic and phylogenetic approach to investigate the origin and evolution of nine major plant hormone (abscisic acid, auxin, b...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.114.247403

    authors: Wang C,Liu Y,Li SS,Han GZ

    更新日期:2015-03-01 00:00:00

  • The formation and function of plant cuticles.

    abstract::The plant cuticle is an extracellular hydrophobic layer that covers the aerial epidermis of all land plants, providing protection against desiccation and external environmental stresses. The past decade has seen considerable progress in assembling models for the biosynthesis of its two major components, the polymer cu...

    journal_title:Plant physiology

    pub_type: 杂志文章,评审

    doi:10.1104/pp.113.222737

    authors: Yeats TH,Rose JK

    更新日期:2013-09-01 00:00:00

  • Physiological responses of soybean plants grown in a nitrogen-free or energy limited environment.

    abstract::Soybean (Glycine max [L.] Merr.) seedlings grown in the absence of combined N and in an Ar:O(2) (79:21, volume/volume) atmosphere had greater seedling and nodule mass, threefold higher acetylene reducing activity per gram fresh weight nodules, no observable increase in nitrogenase Fe-protein, and a higher energy charg...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.96.1.305

    authors: Zhu YX,Schubert KR,Kohl DH

    更新日期:1991-05-01 00:00:00

  • Alternative Oxidase Activity in Tobacco Leaf Mitochondria (Dependence on Tricarboxylic Acid Cycle-Mediated Redox Regulation and Pyruvate Activation).

    abstract::Transgenic Nicotiana tabacum (cv Petit Havana SR1) containing high levels of mitochondrial alternative oxidase (AOX) protein due to the introduction of a sense transgene(s) of Aox1, the nuclear gene encoding AOX, were used to investigate mechanisms regulating AOX activity. After purification of leaf mitochondria, a la...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.109.2.353

    authors: Vanlerberghe GC,Day DA,Wiskich JT,Vanlerberghe AE,McIntosh L

    更新日期:1995-10-01 00:00:00

  • Male Germ Unit Isolation from Three Tricellular Pollen Species: Brassica oleracea, Zea mays, and Triticum aestivum.

    abstract::A technical procedure is described to follow the in vitro release of the ;male germ unit' and the sperm cells from three tricellular pollen species (Brassica, Zea, and Triticum). The condition of the sperm cell was controlled using light microscopy. In addition, for the first time, the sperm cells viability has been c...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.83.3.464

    authors: Matthys-Rochon E,Vergne P,Detchepare S,Dumas C

    更新日期:1987-03-01 00:00:00

  • An intermediate in the synthesis of glucobrassicins from 3-indoleacetaldoxime by woad leaves.

    abstract::Leaves of woad (Isatis tinctoria L.) were found to incorporate efficiently tritiated indoleacetaldoxime and (35)S from (35)S-l-cystine into glucobrassicin and sulfoglucobrassicin. Time course of incorporation of (35)S from (35)S-cystine into the glucosinolates indicated that glucobrassicin was formed first and then su...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.50.1.43

    authors: Mahadevan S,Stowe BB

    更新日期:1972-07-01 00:00:00

  • Dehydroipomeamarone as an Intermediate in the Biosynthesis of Ipomeamarone, a Phytoalexin from Sweet Potato Root Infected with Ceratocystis fimbriata.

    abstract::Recently, we isolated dehydroipomeamarone, a new sesquiterpenoid from sweet potato (Ipomoea batatas Lam.) root tissue infected with Ceratocystis fimbriata (Ell. et Halst.). The purpose of this investigation was to determine whether dehydroipomeamarone was a precursor in the biosynthetic pathway of ipomeamarone. The in...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.53.4.649

    authors: Oguni I

    更新日期:1974-04-01 00:00:00

  • Purification and characterization of mitochondrial citrate synthase.

    abstract::Mitochondrial citrate synthase was purified from leaves of Pisum sativum L. cv Progress 9. A three step purification was employed using ATP-Sepharose affinity chromatography which resulted in a 600-fold enrichment. Enzyme activity was assayed spectrophotometrically during greening of etiolated leaves under constant wh...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.89.3.719

    authors: Unger EA,Vasconcelos AC

    更新日期:1989-03-01 00:00:00

  • Transformation of Soybean Cells Using Mixed Strains of Agrobacterium tumefaciens and Phenolic Compounds.

    abstract::Cotyledon explants from germinated 1-day-old soybean seedling were inoculated with single or mixed strains of Agrobacterium tumefaciens. Mixed-strain infections with the supervirulent L,L-succinamopine type strain A281 (pTiBo542) and strain LBA4404 carrying an octopine type virulence (vir) region and a binary vector (...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.88.3.570

    authors: Owens LD,Smigocki AC

    更新日期:1988-11-01 00:00:00

  • Selenium absorption by excised Astragalus roots.

    abstract::Absorption of selenate and selenite by excised roots of Astragalus Crotalariae, a selenium accumulator, and of A. lentiginosus, a non-accumulator, was favored by CaCl(2) and a pH of 4.0. The uptake of selenate and possibly selenite, is metabolically linked. Roots of a number of Astragalus species were examined, and in...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.43.1.14

    authors: Ulrich JM,Shrift A

    更新日期:1968-01-01 00:00:00

  • Sucrose synthase is associated with the cell wall of tobacco pollen tubes.

    abstract::Sucrose synthase (Sus; EC 2.4.1.13) is a key enzyme of sucrose metabolism in plant cells, providing carbon for respiration and for the synthesis of cell wall polymers and starch. Since Sus is important for plant cell growth, insights into its structure, localization, and features are useful for defining the relationsh...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.108.115956

    authors: Persia D,Cai G,Del Casino C,Faleri C,Willemse MT,Cresti M

    更新日期:2008-08-01 00:00:00