Molecular cloning and expression analysis of the mitochondrial pyruvate dehydrogenase from maize.

Abstract:

:Four cDNAs, one encoding an alpha-subunit and three encoding beta-subunits of the mitochondrial pyruvate dehydrogenase, were isolated from maize (Zea mays L.) libraries. The deduced amino acid sequences of both alpha- and beta-subunits are approximately 80% identical with Arabidopsis and pea (Pisum sativum L.) homologs. The mature N terminus was determined for the beta-subunit by microsequencing the protein purified from etiolated maize shoot mitochondria and was resolved by two-dimensional gel electrophoresis. This single isoelectric species comprised multiple isoforms. Both alpha- and beta-subunits are encoded by multigene families in maize, as determined by Southern-blot analyses. RNA transcripts for both alpha- and beta-subunits were more abundant in roots than in young leaves or etiolated shoots. Pyruvate dehydrogenase activity was also higher in roots (5-fold) compared with etiolated shoots and leaves. Both subunits were present at similar levels in all tissues examined, indicating coordinated gene regulation. The protein levels were highest in heterotrophic organs and in pollen, which contained about 2-fold more protein than any other organ examined. The relative abundance of these proteins in nonphotosynthetic tissues may reflect a high cellular content of mitochondria, a high level of respiratory activity, or an extra plastidial requirement for acetate.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Thelen JJ,Miernyk JA,Randall DD

doi

10.1104/pp.119.2.635

subject

Has Abstract

pub_date

1999-02-01 00:00:00

pages

635-44

issue

2

eissn

0032-0889

issn

1532-2548

journal_volume

119

pub_type

杂志文章
  • Defects in a new class of sulfate/anion transporter link sulfur acclimation responses to intracellular glutathione levels and cell cycle control.

    abstract::We previously identified a mutation, suppressor of mating type locus3 15-1 (smt15-1), that partially suppresses the cell cycle defects caused by loss of the retinoblastoma tumor suppressor-related protein encoded by the MAT3 gene in Chlamydomonas reinhardtii. smt15-1 single mutants were also found to have a cell cycle...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.114.251009

    authors: Fang SC,Chung CL,Chen CH,Lopez-Paz C,Umen JG

    更新日期:2014-12-01 00:00:00

  • Phosphatidylinositol 4,5-Bisphosphate Phospholipase C and Phosphomonoesterase in Dunaliella salina Membranes.

    abstract::In comparison with other cell organelles, the Dunaliella salina plasma membrane was found to be highly enriched in phospholipase C activity toward exogenous [(3)H]phosphatidylinositol 4,5-bisphosphate (PIP(2)). Based on release of [(3)H]inositol phosphates, the plasma membrane exhibited a PIP(2)-phospholipase C activi...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.90.3.1115

    authors: Einspahr KJ,Peeler TC,Thompson GA

    更新日期:1989-07-01 00:00:00

  • The SPOROCYTELESS/NOZZLE gene is involved in controlling stamen identity in Arabidopsis.

    abstract::The stamen, which consists of an anther and a filament, is the male reproductive organ in a flower. The specification of stamen identity in Arabidopsis (Arabidopsis thaliana) is controlled by a combination of the B genes APETALA3 (AP3) and PISTILLATA, the C gene AGAMOUS (AG), and the E genes SEPALLATA1 (SEP1) to SEP4....

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.109.145896

    authors: Liu X,Huang J,Parameswaran S,Ito T,Seubert B,Auer M,Rymaszewski A,Jia G,Owen HA,Zhao D

    更新日期:2009-11-01 00:00:00

  • Xylem Sap Surface Tension May Be Crucial for Hydraulic Safety.

    abstract::The surface tension (γ) of xylem sap plays a key role in stabilizing air-water interfaces at the pits between water- and gas-filled conduits to avoid air seeding at low water potentials. We studied seasonal changes in xylem sap γ in Picea abies and Pinus mugo growing at the alpine timberline. We analyzed their vulnera...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.17.01053

    authors: Losso A,Beikircher B,Dämon B,Kikuta S,Schmid P,Mayr S

    更新日期:2017-11-01 00:00:00

  • Sequence and comparative analysis of the maize NB mitochondrial genome.

    abstract::The NB mitochondrial genome found in most fertile varieties of commercial maize (Zea mays subsp. mays) was sequenced. The 569,630-bp genome maps as a circle containing 58 identified genes encoding 33 known proteins, 3 ribosomal RNAs, and 21 tRNAs that recognize 14 amino acids. Among the 22 group II introns identified,...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.104.044602

    authors: Clifton SW,Minx P,Fauron CM,Gibson M,Allen JO,Sun H,Thompson M,Barbazuk WB,Kanuganti S,Tayloe C,Meyer L,Wilson RK,Newton KJ

    更新日期:2004-11-01 00:00:00

  • Water is allocated differently to chloroplasts in sun and shade leaves.

    abstract::Hydrogen-1 nuclear magnetic resonance spectroscopy was used to study water allocation in cell compartments of sun and shade leaves. NMR spectra of Acer platanoides were resolved into two peaks that were assigned to chloroplast and nonchloroplast water. Sun leaves contained 1.7 times more water per unit area of surface...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.86.1.16

    authors: McCain DC,Croxdale J,Markley JL

    更新日期:1988-01-01 00:00:00

  • Tonoplast vesicles of opposite sidedness from soybean hypocotyls by preparative free-flow electrophoresis.

    abstract::Tonoplast vesicles were purified from a microsomal fraction isolated from etiolated soybean hypocotyls (Glycine max L.) by preparative free-flow electrophoresis. Marker enzyme determinations and immunoblot analysis against the vacuolar-ATPase confirmed the nature and the purity of the isolated membranes. A purified to...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.94.3.1149

    authors: Canut H,Brightman A,Boudet AM,Morré DJ

    更新日期:1990-11-01 00:00:00

  • Comparative analysis of SET domain proteins in maize and Arabidopsis reveals multiple duplications preceding the divergence of monocots and dicots.

    abstract::Histone proteins play a central role in chromatin packaging, and modification of histones is associated with chromatin accessibility. SET domain [Su(var)3-9, Enhancer-of-zeste, Trithorax] proteins are one class of proteins that have been implicated in regulating gene expression through histone methylation. The relatio...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.102.013722

    authors: Springer NM,Napoli CA,Selinger DA,Pandey R,Cone KC,Chandler VL,Kaeppler HF,Kaeppler SM

    更新日期:2003-06-01 00:00:00

  • Two-Dimensional Electrophoretic Studies of the Proteins and Polypeptides in Mature Pollen Grains and the Male Germ Unit of Plumbago zeylanica.

    abstract::Three fractions (male germ unit [MGU]-rich, cytoplasmic-particulate and water-soluble proteins) were isolated from pollen of Plumbago zeylanica L. Proteins were extracted using a phenol procedure and polypeptide patterns were compared on one- and two-dimensional polyacrylamide gels. The MGU-rich fraction contains the ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.88.3.764

    authors: Geltz NR,Russell SD

    更新日期:1988-11-01 00:00:00

  • Histochemical and biochemical observations on storage protein metabolism and protein body autolysis in cotyledons of germinating mung beans.

    abstract::Storage protein hydrolysis in the cotyledons of germinating mung beans (Phaseolus aureus Roxb.) was examined by histochemical techniques, and the autolytic capacity of isolated protein bodies was studied with biochemical methods. The localization of endopeptidase activity within the cotyledons was studied using an Ind...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.56.2.292

    authors: Harris N,Chrispeels MJ

    更新日期:1975-08-01 00:00:00

  • Regulation of Assimilatory Sulfate Reduction by Herbicide Safeners in Zea mays L.

    abstract::Effects of the herbicide safeners N,N-diallyl-2,2-dichloroacetamide and 4-dichloroacetyl-3,4-dihydro-3-methyl-2H-1,4-benzooxazin (CGA 154281) on the contents in cysteine and glutathione, on the assimilation of (35)SO(4) (2-), and on the enzymes of assimilatory sulfate reduction were analyzed in roots and primary leave...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.94.4.1808

    authors: Farago S,Brunold C

    更新日期:1990-12-01 00:00:00

  • A Role for the F-Box Protein HAWAIIAN SKIRT in Plant microRNA Function.

    abstract::As regulators of gene expression in multicellular organisms, microRNAs (miRNAs) are crucial for growth and development. Although a plethora of factors involved in their biogenesis and action in Arabidopsis (Arabidopsis thaliana) has been described, these processes and their fine-tuning are not fully understood. Here, ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.17.01313

    authors: Lang PLM,Christie MD,Dogan ES,Schwab R,Hagmann J,van de Weyer AL,Scacchi E,Weigel D

    更新日期:2018-01-01 00:00:00

  • OsNAR2.1 Interaction with OsNIT1 and OsNIT2 Functions in Root-growth Responses to Nitrate and Ammonium.

    abstract::The nitrate transport accessory protein OsNAR2 plays a critical role in root-growth responses to nitrate and nitrate acquisition in rice (Oryza sativa). In this study, a pull-down assay combined with yeast two-hybrid and coimmunoprecipitation analyses revealed that OsNAR2.1 interacts with OsNIT1 and OsNIT2. Moreover, ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.19.01364

    authors: Song M,Fan X,Chen J,Qu H,Luo L,Xu G

    更新日期:2020-05-01 00:00:00

  • Rapid Hormone-induced Hyperpolarization of the Oat Coleoptile Transmembrane Potential.

    abstract::The effects of the plant growth substances indoleacetic acid (IAA) and fusicoccin on the transmembrane potential of Avena coleoptile cells (at 27-29 C) were studied. Fusicoccin caused hyperpolarization of the membrane potential which started after a lag of less than 20 seconds, and which on average reached -49 mv at a...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.59.3.395

    authors: Cleland RE,Prins HB,Harper JR,Higinbotham N

    更新日期:1977-03-01 00:00:00

  • Adaptation of the Cyanobacterium Anabaena variabilis to Low CO(2) Concentration in Their Environment.

    abstract::The rate of adaptation of high CO(2) (5% v/v CO(2) in air)-grown Anabaena to a low level of CO(2) (0.05% v/v in air) was determined as a function of O(2) concentration. Exposure of cells to low (2.6%) O(2) concentration resulted in an extended lag in the adaptation to low CO(2) concentration. The rate of adaptation fo...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.71.1.208

    authors: Marcus Y,Harel E,Kaplan A

    更新日期:1983-01-01 00:00:00

  • The hpa1 mutant of Arabidopsis reveals a crucial role of histidine homeostasis in root meristem maintenance.

    abstract::Histidine (His) is an essential ingredient for protein synthesis and is required by all living organisms. In higher plants, although there is considerable evidence that His is essential for plant growth and survival, there is very little information as to whether it plays any specific role in plant development. Here, ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.106.084178

    authors: Mo X,Zhu Q,Li X,Li J,Zeng Q,Rong H,Zhang H,Wu P

    更新日期:2006-08-01 00:00:00

  • Endoplasmic Reticulum Formation during Germination of Wheat Seeds : A QUANTITATIVE ELECTRON MICROSCOPE STUDY.

    abstract::This study demonstrates germination-induced ultrastructural changes in wheat (Triticum aestivum L. cv Arthur) aleurone cells. Seeds imbided for 4 hours in water contained endoplasmic reticulum (ER) or ER-like membranes as vesicles or as short segments of membrane associated with the spherosomes on the periphery of ale...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.68.6.1319

    authors: Buckhout TJ,Gripshover BM,Morré DJ

    更新日期:1981-12-01 00:00:00

  • Gibberellin mobilizes distinct DELLA-dependent transcriptomes to regulate seed germination and floral development in Arabidopsis.

    abstract::Severe Arabidopsis (Arabidopsis thaliana) gibberellin (GA)-deficient mutant ga1-3 fails to germinate and is impaired in floral organ development. In contrast, the ga1-3 gai-t6 rga-t2 rgl1-1 rgl2-1 mutant confers GA-independent seed germination and floral development. This fact suggests that GA-regulated transcriptomes...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.106.082289

    authors: Cao D,Cheng H,Wu W,Soo HM,Peng J

    更新日期:2006-10-01 00:00:00

  • Osmoregulation in Cotton in Response to Water Stress : II. LEAF CARBOHYDRATE STATUS IN RELATION TO OSMOTIC ADJUSTMENT.

    abstract::Diurnal changes in tissue water potential components, photosynthesis, and specific leaf carbohydrates were examined in water stress-adapted and nonadapted cotton plants. Adapted plants exhibited lower daily minimum leaf water potentials and maintained turgor to lower leaf water potentials than nonadapted plants. Becau...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.67.3.489

    authors: Ackerson RC

    更新日期:1981-03-01 00:00:00

  • Coordinate changes in carbon partitioning and plastidial metabolism during the development of oilseed rape embryos.

    abstract::Measurements of metabolic fluxes in whole embryos and isolated plastids have revealed major changes in the pathways of carbon utilization during cotyledon filling by oilseed rape (Brassica napus L.) embryos. In the early cotyledon stage (stage A), embryos used sucrose (Suc) predominantly for starch synthesis. Plastids...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.122.3.767

    authors: Eastmond PJ,Rawsthorne S

    更新日期:2000-03-01 00:00:00

  • A cross-polarization, magic-angle-spinning, 13C-nuclear-magnetic-resonance study of polysaccharides in sugar beet cell walls

    abstract::Solid-state nuclear magnetic resonance relaxation experiments were used to study the rigidity and spatial proximity of polymers in sugar beet (Beta vulgaris) cell walls. Proton T1rho decay and cross-polarization patterns were consistent with the presence of rigid, crystalline cellulose microfibrils with a diameter of ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.119.4.1315

    authors: Renard CM,Jarvis MC

    更新日期:1999-04-01 00:00:00

  • Subcellular localization of a high affinity binding site for D-myo-inositol 1,4,5-trisphosphate from Chenopodium rubrum.

    abstract::It is now generally accepted that a phosphoinositide cycle is involved in the transduction of a variety of signals in plant cells. In animal cells, the binding of D-myo-inositol 1,4,5-trisphosphate (InsP(3)) to a receptor located on the endoplasmic reticulum (ER) triggers an efflux of calcium release from the ER. Site...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.124.1.475

    authors: Martinec J,Feltl T,Scanlon CH,Lumsden PJ,Machácková I

    更新日期:2000-09-01 00:00:00

  • The sugar-dependent1 lipase limits triacylglycerol accumulation in vegetative tissues of Arabidopsis.

    abstract::There has been considerable interest recently in the prospect of engineering crops to produce triacylglycerol (TAG) in their vegetative tissues as a means to achieve a step change in oil yield. Here, we show that disruption of TAG hydrolysis in the Arabidopsis (Arabidopsis thaliana) lipase mutant sugar-dependent1 (sdp...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.113.219840

    authors: Kelly AA,van Erp H,Quettier AL,Shaw E,Menard G,Kurup S,Eastmond PJ

    更新日期:2013-07-01 00:00:00

  • Salicylic Acid in Rice (Biosynthesis, Conjugation, and Possible Role).

    abstract::Salicylic acid (SA) is a natural inducer of disease resistance in some dicotyledonous plants. Rice seedlings (Oryza sativa L.) had the highest levels of SA among all plants tested for SA content (between 0.01 and 37.19 [mu]g/g fresh weight). The second leaf of rice seedlings had slightly lower SA levels than any young...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.108.2.633

    authors: Silverman P,Seskar M,Kanter D,Schweizer P,Metraux JP,Raskin I

    更新日期:1995-06-01 00:00:00

  • Activation of Chloroplast NADP-linked Glyceraldehyde-3-Phosphate Dehydrogenase by the Ferredoxin/Thioredoxin System.

    abstract::NADP-glyceraldehyde-3-P dehydrogenase of spinach (Spinacia oleracea) chloroplasts was activated by thioredoxin that was reduced either photochemically with ferredoxin and ferredoxin-thioredoxin reductase or chemically with dithiothreitol. The activation process that was observed with the soluble protein fraction from ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.61.4.669

    authors: Wolosiuk RA,Buchanan BB

    更新日期:1978-04-01 00:00:00

  • Calcium-regulated proteolysis of eEF1A.

    abstract::Eukaryotic elongation factor 1alpha (eEF1A) can be post-translationally modified by the addition of phosphorylglycerylethanolamine (PGE). [(14)C]Ethanolamine was incorporated into the PGE modification, and with carrot (Daucus carota L.) suspension culture cells, eEF1A was the only protein that incorporated detectable ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.122.3.957

    authors: Ransom-Hodgkins WD,Brglez I,Wang X,Boss WF

    更新日期:2000-03-01 00:00:00

  • The Arabidopsis aleurone layer responds to nitric oxide, gibberellin, and abscisic acid and is sufficient and necessary for seed dormancy.

    abstract::Seed dormancy is a common phase of the plant life cycle, and several parts of the seed can contribute to dormancy. Whole seeds, seeds lacking the testa, embryos, and isolated aleurone layers of Arabidopsis (Arabidopsis thaliana) were used in experiments designed to identify components of the Arabidopsis seed that cont...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.106.093435

    authors: Bethke PC,Libourel IG,Aoyama N,Chung YY,Still DW,Jones RL

    更新日期:2007-03-01 00:00:00

  • Structure-activity relationship in the auxin activity of mono-substituted phenylacetic acids.

    abstract::The analysis of substituent constants for the lipophilic and electronic factors in the auxin activity of substituted phenylacetic acids in elongation of coleoptile segments shows that these factors parallel those for the phenoxyacetic acids but assign reactivity in growth promotion to the meta position of phenylacetic...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.42.11.1519

    authors: Muir RM,Fujita T,Hansch C

    更新日期:1967-11-01 00:00:00

  • Changes in the Level of [C]Indole-3-Acetic Acid and [C]Indoleacetylaspartic Acid during Root Formation in Mung Bean Cuttings.

    abstract::Changes in the levels of [(14)C]indole-3-acetic acid (IAA) and [(14)C]indole-acetylaspartic acid (IAAsp) were examined during adventitious root formation in mung bean (Vigna radiata [L.] R. Wilcz. ;Berken') stem cuttings. IAAsp was identified by GC-MS as the primary conjugate in IAA-treated cuttings. During root forma...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.86.4.1236

    authors: Norcini JG,Heuser CW

    更新日期:1988-04-01 00:00:00

  • OsmiR396d Affects Gibberellin and Brassinosteroid Signaling to Regulate Plant Architecture in Rice.

    abstract::Genetic improvement of plant architecture is one of the strategies for increasing the yield potential of rice (Oryza sativa). Although great progress has been made in the understanding of plant architecture regulation, the precise mechanism is still an urgent need to be revealed. Here, we report that over-expression o...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.17.00964

    authors: Tang Y,Liu H,Guo S,Wang B,Li Z,Chong K,Xu Y

    更新日期:2018-01-01 00:00:00