Cytosolic triosephosphate isomerase is a single gene in rice.

Abstract:

:A cDNA clone encoding rice (Oryza sativa L.) cytosolic triosephosphate isomerase (TPI), an important glycolytic enzyme, was isolated and characterized. The clone (pRTPI-6) contains an open reading frame of 759 base pairs, encoding a polypeptide chain of 253 amino acid residues (M(r) 27,060). The identity of this clone was defined by its high homology (85% nucleotide sequence and 89% amino acid sequence identical match) with a maize mRNA sequence encoding the cytosolic TPI and with TPIs from other species. Genomic DNA blot analysis using the cDNA as a probe showed that the cytosolic TPI gene is present as a single copy per haploid rice genome, as opposed to that found in maize, in which multiple TPI gene copies exist. A single TPI mRNA species of about 1100 nucleotides was detected by gel blot hybridization analysis of RNA isolated from root, culm, and leaf tissues, indicating that its expression is ubiquitous. Based on sequence comparison and molecular analysis, we propose that the chloroplast-located TPI may be encoded by divergent structural nuclear genes in rice.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Xu Y,Hall TC

doi

10.1104/pp.101.2.683

subject

Has Abstract

pub_date

1993-02-01 00:00:00

pages

683-7

issue

2

eissn

0032-0889

issn

1532-2548

pii

101/2/683

journal_volume

101

pub_type

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