Cytochemical and cytofluorometric evidence for guard cell photosystems.

Abstract:

:Evidence for photosynthetic linear electron transport in guard cells was obtained with two sensitive methods of high spacial resolution. Light-dependent diaminobenzidine oxidation (an indicator of PSI) and DCMU-sensitive, light-dependent thiocarbamyl nitroblue tetrazolium reduction (an indicator of PSII) were observed in guard cell plastids of Hordeum vulgare L. cv Himalaya using electron microscopic cytochemical procedures. DCMU-sensitive Chl a fluorescence induction (an indicator of PSII) was detected in individual guard cell pairs of Vicia faba L. cv Longpod using an ultramicrofluorometer. At least for these species, we conclude these results are proof for the presence of PSII in guard cell chloroplasts, which until now has been somewhat controversial.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Vaughn KC,Outlaw WH

doi

10.1104/pp.71.2.420

subject

Has Abstract

pub_date

1983-02-01 00:00:00

pages

420-4

issue

2

eissn

0032-0889

issn

1532-2548

journal_volume

71

pub_type

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