The ribosomes of pear fruit. Their synthesis during the climacteric and the age-related compensatory response to ionizing radiation.

Abstract:

:Maceration at liquid nitrogen temperatures, use of poly-vinylpyrrolidone, and careful pH control are essential to the isolation of ribosomes and polysomes from deciduous fruit tissue. Characteristics of the ribosomes and constituent RNA are described. The distribution of ribosomes among monomer and polymer forms remains relatively constant until fruit reach the climacteric peak, after which there is a notable decline in the polymeric forms. In contrast to the relative stability in size distribution there is a marked change in ribosomal turnover during the climacteric rise. A transitory increase in rate of ribosomal synthesis is followed by a rapid decline coincident with the final portion of the climacteric rise. No incorporation of radioactive base into ribosomes could be detected once fruit had reached the climacteric peak.Coincidence of radioactivity with the ribosomal RNA on methylated albumin-Kieselguhr chromatograms and complete inhibition of ribosomal RNA synthesis by actinomycin D confirm that radioactive nucleotide was incorporated into newly synthesized ribosomes. Data are presented to distinguish between a cellular response to injury, as may result from the preparation of tissue slices, and the effects of physiological age. Superimposed intracellular radiation injury stimulates the synthesis of new ribosomes and underscores a major transition in the dynamics of the ribosomal system coincident with the climacteric rise.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Ku LL,Romani RJ

doi

10.1104/pp.45.4.401

subject

Has Abstract

pub_date

1970-04-01 00:00:00

pages

401-7

issue

4

eissn

0032-0889

issn

1532-2548

journal_volume

45

pub_type

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