Abstract:
:The interaction between kinetin and naphthaleneacetic acid in the regulation of senescence of excised tissue of mature broccoli leaves has been used to examine the extent of synchrony between changes in chlorophyll, RNA, and protein. Kinetin increased the net uptake of (14)C-labeled orotic acid and leucine. Naphthaleneacetic acid decreased the effect of kinetin on net uptake after long treatment, but in short-time treatments the auxin increased the effect of kinetin on net uptake. Results of long (24 hr) treatments indicated a general synchrony between the loss of RNA, protein, and chlorophyll. Naphthaleneacetic acid reduced the stabilizing effect of kinetin upon chlorophyll content and upon the content and synthesis of RNA. In short-time experiments, however, RNA content and synthesis were transiently increased by kinetin, and further increased by kinetin plus naphthaleneacetic acid, while chlorophyll content decreased in the presence of kinetin and decreased further in the presence of kinetin plus naphthaleneacetic acid. Actinomycin-D accelerated the loss of chlorophyll, RNA and protein and strongly depressed the rate of RNA synthesis. In the presence of actinomycin-D the stabilizing effect of kinetin upon RNA was substantially reduced. In contrast, the chlorophyll and protein contents remained higher than in the control. Actinomycin-D did not nullify the basal incorporation of orotic acid into RNA, nor did it negate the effect of kinetin upon incorporation. The failure of synchrony between changes in chlorophyll and RNA does not substantiate the proposal that kinetin regulates senescence by a direct effect upon DNA-dependent RNA synthesis.
journal_name
Plant Physioljournal_title
Plant physiologyauthors
VonAbrams GJ,Pratt HKdoi
10.1104/pp.43.8.1271subject
Has Abstractpub_date
1968-08-01 00:00:00pages
1271-8issue
8eissn
0032-0889issn
1532-2548journal_volume
43pub_type
杂志文章相关文献
PLANT PHYSIOLOGY文献大全abstract::trans-Acting small interfering RNAs (tasiRNAs) participate in the regulation of organ morphogenesis and determination of developmental timing in plants by down-regulating target genes through mRNA cleavage. The production of tasiRNAs is triggered by microRNA173 (miR173) and other specific microRNA-mediated cleavage of...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.16.00148
更新日期:2016-05-01 00:00:00
abstract::Plants respond in complex ways to their environment, to their internal physiological status, and to the activity of other plants, pathogens, herbivores, and organisms. Plant Signaling 2000, a symposium sponsored by the Penn State Intercollege Graduate Program in Plant Physiology (May 18-20, 2000), explored the machine...
journal_title:Plant physiology
pub_type:
doi:10.1104/pp.124.2.499
更新日期:2000-10-01 00:00:00
abstract::Treatment of Pisum sativum (L.) cv. ;Sparkle' with ethyl methanesulfonic acid (EMS) produced a stable mutant, E135F, which forms small, white, ineffective nodules. These nodules exhibit histological zonation typical of an indeterminant nodule, e.g. meristematic, early symbiotic, late symbiotic, and senescent zones. Co...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.94.3.899
更新日期:1990-11-01 00:00:00
abstract::Inactivation of inward-rectifying K+ channels (IK,in) by a rise in cytosolic free [Ca2+] ([Ca2+]i) is a key event leading to solute loss from guard cells and stomatal closure. However, [Ca2+]i action on IK,in has never been quantified, nor are its origins well understood. We used membrane voltage to manipulate [Ca2+]i...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.119.1.277
更新日期:1999-01-01 00:00:00
abstract::Biochemical and electrophysiological studies on plant vacuolar transporters became feasible in the late 1970s and early 1980s, when methods to isolate large quantities of intact vacuoles and purified vacuolar membrane vesicles were established. However, with the exception of the H+-ATPase and H+-PPase, which could be ...
journal_title:Plant physiology
pub_type: 杂志文章,评审
doi:10.1104/pp.17.01481
更新日期:2018-02-01 00:00:00
abstract::Addition of excess copper to nongrowing cells of a normal, green Chlorella caused a reduction in total pigments and a blue shift of chlorophyll absorption, concurrent with the inhibition of photosynthesis. Chlorophylless yellow and white mutant strains of the same alga showed a rise in nonspecific absorption (i.e., ch...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.46.2.183
更新日期:1970-08-01 00:00:00
abstract::Experiments were designed to test the hypothesis that the labeled products recovered from plant tissue incubated with [(14)C]GA(12)-7-aldehyde ([(14)C]GA(12)ald) would serve as appropriate [(14)C]markers for the recovery of naturally-occurring gibberellins (GAs). The [(14)C]GA(12)ald (about 200 millicuries per millimo...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.81.4.984
更新日期:1986-08-01 00:00:00
abstract::A recognition molecule was isolated from stigmas of S-allele genotype S(2)S(2) of Brassica oleracea var. capitata L. After Sephadex chromatography, it eluted as a single symmetrical peak during diethylaminoethane-cellulose chromatography. A high degree of purity was affirmed by: sedimentation as a single peak during u...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.67.2.270
更新日期:1981-02-01 00:00:00
abstract::Stimulation of stem elongation on green cuttings of Phaseolus aureus by malformin occurred only in red light and was specifically reversible by subsequent treatment with far red radiation. Inhibition of stem elongation of etiolated cuttings by malformin in the dark was alleviated by red light and was repeatedly revers...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.60.4.622
更新日期:1977-10-01 00:00:00
abstract::The role of polygalacturonase in leaf abscission was studied in explants of Phaseolus vulgaris L. cv. Red Kidney. Bean polygalacturonase was partially characterized and comparisons were made between the bean enzyme and previously reported higher plant polygalacturonases. Polygalacturonase isolated from bean leaf absci...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.63.6.1133
更新日期:1979-06-01 00:00:00
abstract::Geranylgeranyl diphosphate (GGPP) is the precursor for the biosynthesis of gibberellins, carotenoids, chlorophylls, isoprenoid quinones, and geranylgeranylated proteins in plants. There is a small gene family for GGPP synthases encoding five isozymes and one related protein in Arabidopsis, and all homologs have a puta...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.122.4.1045
更新日期:2000-04-01 00:00:00
abstract::Using tissue blotting and immunocytolocalization, we have investigated the appearance and accumulation of polygalacturonase (PG) during tomato (Lycopersicon esculentum Mill.) fruit ripening. Results show that PG first appears in the collumella region followed by sequential appearance in the exopericarp and endopericar...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.90.1.17
更新日期:1989-05-01 00:00:00
abstract::The problem of determining gas exchange rates from flow system data under nonsteady state conditions is analyzed. A correction factor is presented for obtaining constant rates under nonsteady state conditions. A general formula for obtaining any rate under nonsteady state conditions is also given. Turnover time is def...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.56.5.680
更新日期:1975-11-01 00:00:00
abstract::Serial analysis of gene expression was used to profile transcript levels in Arabidopsis roots and assess their responses to 2,4,6-trinitrotoluene (TNT) exposure. SAGE libraries representing control and TNT-exposed seedling root transcripts were constructed, and each was sequenced to a depth of roughly 32,000 tags. Mor...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.103.028019
更新日期:2003-11-01 00:00:00
abstract::Polysaccharide analyses of mutants link several of the glycosyltransferases encoded by the 10 CesA genes of Arabidopsis to cellulose synthesis. Features of those mutant phenotypes point to particular genes depositing cellulose predominantly in either primary or secondary walls. We used transformation with antisense co...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.010931
更新日期:2002-06-01 00:00:00
abstract::The course of senescence in the first leaves of light-grown Avena seedlings when attached to the plant has been compared with that previously studied in detached leaves and leaf segments. Proteolysis in the leaf, whether attached or detached, is accompanied by markedly polar basipetal transport of amino acids. This po...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.54.6.859
更新日期:1974-12-01 00:00:00
abstract::Xyloglucan is an important hemicellulosic polysaccharide in dicot primary cell walls. Most of the enzymes involved in xyloglucan synthesis have been identified. However, many important details of its synthesis in vivo remain unknown. The roles of three genes encoding xylosyltransferases participating in xyloglucan bio...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.112.198119
更新日期:2012-08-01 00:00:00
abstract::Seeds with physiological dormancy usually experience primary and secondary dormancy in the nature; however, little is known about the differential regulation of primary and secondary dormancy. We combined multiple approaches to investigate cytological changes, hormonal levels, and gene expression dynamics in Cunningha...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.16.00384
更新日期:2016-12-01 00:00:00
abstract::Protein dephosphorylation by the serine/threonine protein phosphatase 2A (PP2A) modulates a broad array of cellular functions. PP2A normally acts as a heterotrimeric holoenzyme complex comprising a catalytic subunit bound by regulatory A and B subunits. Characterization of the regulatory A subunit isoforms (ROOTS CURL...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.107.112995
更新日期:2008-02-01 00:00:00
abstract::The occurrence of the complete orotic acid pathway for the biosynthesis de novo of pyrimidine nucleotides was demonstrated in the intact cells of roots excised from summer squash (Cucurbita pepo L. cv. Early Prolific Straightneck). Evidence that the biosynthesis of pyrimidine nucleotides proceeds via the orotate pathw...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.64.4.562
更新日期:1979-10-01 00:00:00
abstract::Intact spinach and barley chloroplast normally incorporate (14)C-acetate into palmitate and oleate as the major (14)C fatty acids. Addition of nitrite markedly altered the relative patterns of the products with the appearance of stearate, a drop in oleate, but no marked change in palmitate. Arsenite greatly increased ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.49.4.497
更新日期:1972-04-01 00:00:00
abstract::The release of fatty acids from membrane lipids has been implicated in various metabolic and physiological processes, but in many cases, the enzymes involved and their functions in plants remain unclear. Patatin-related phospholipase As (pPLAs) constitute a major family of acyl-hydrolyzing enzymes in plants. Here, we ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.113.216994
更新日期:2013-05-01 00:00:00
abstract::Cells of the unicellular green algae Chlamydomonas reinhardtii were grown in high dissolved inorganic carbon (DIC) concentrations (supplied with 50 milliliters per liter CO(2)[g]) and transferred to low DIC concentrations (supplied with = 100 microliters per liter CO(2)[g]). Immediately after transfer from high to l...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.94.1.357
更新日期:1990-09-01 00:00:00
abstract::The level of unsaturation of the constituent fatty acids of many glycerolipids in plant membranes is modified by environmental factors. The measurement of the rate of the desaturation of these fatty acids is essential to an understanding of how plants adapt to changing environments. This is difficult because of the co...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.101.3.977
更新日期:1993-03-01 00:00:00
abstract::The ripening of a fleshy fruit represents the summation of an array of biochemical processes that are regulated by interactions between developmental programs and environmental inputs. Analysis of tomato (Solanum lycopersicum) mutants and inhibitor studies indicate that ethylene is necessary for full development of th...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.104.057745
更新日期:2005-05-01 00:00:00
abstract::Young seedlings of Lavatera cretica L. exhibit positive phototropism. The hypocotyl perceives unilateral illumination with blue light and curves towards the light source by unequal growth. In addition, the cotyledonary laminas perceive the vectorial component of unilateral illumination with blue light and reorient nor...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.66.1.82
更新日期:1980-07-01 00:00:00
abstract::The relationship between the state of oligomerization and activity of purified maize leaf phosphoenolpyruvate carboxylase using size exclusion high performance liquid chromatography was examined. Maximum activities of 35 to 38 micromoles per minute per milligram protein were found when 100% of the enzyme was in its te...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.80.4.848
更新日期:1986-04-01 00:00:00
abstract::In Rhodella violacea phycoerythrin (PE) has two transcripts, a premessenger and a mature messenger (the gene contains an intron). Phycocyanin, which is plastid-encoded, and the 33-kD PE rod linker polypeptide, which is nuclear-encoded, have only one transcript. The PE premessenger had a rapid turnover; mature transcri...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.112.3.1045
更新日期:1996-11-01 00:00:00
abstract::Mutations at the glossy1 (gl1) locus of maize (Zea mays L.) quantitatively and qualitatively affect the deposition of cuticular waxes on the surface of seedling leaves. The gl1 locus has been molecularly cloned by transposon tagging with the Mutator transposon system. The epi23 cDNA was isolated by subtractive hybridi...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.113.4.1091
更新日期:1997-04-01 00:00:00
abstract::The alternative oxidase (AOX) pathway of plant mitochondria uncouples respiration from mitochondrial ATP production and may ameliorate plant performance under stressful environmental conditions, such as cold temperatures, by preventing excess accumulation of reactive oxygen species. We tested this model in whole tissu...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.105.070789
更新日期:2005-12-01 00:00:00