Regulation of Plant Acetyl-CoA Carboxylase by Adenylate Nucleotides.

Abstract:

:The assay of acetyl-CoA carboxylase (EC 6.4.1.2) does not follow ideal zero-order kinetics when assayed in a crude extract from wheat (Triticum aestivum L.) germ. Our results show that the lack of ideality is the consequence of contamination by ATPase and adenylate kinase. These enzyme activities generate significant amounts of ADP and AMP in the assay mixture, thus limiting the availability of ATP for the carboxylase reaction. Moreover, ADP and AMP are competitive inhibitors, with respect to ATP, of acetyl-CoA carboxylase. Similar relationships between adenylate nucleotides and acetyl-CoA carboxylase are found in isolated chloroplasts. There is no evidence that acetyl-CoA carboxylase activity in the extracts of the plant systems examined is altered by covalent modification, such as a phosphorylation-dephosphorylation cycle. A scheme is presented that illustrates the dependency of acetyl-CoA carboxylase and fatty acid synthesis on the energy demands of the chloroplasts in vivo.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Eastwell KC,Stumpf PK

doi

10.1104/pp.72.1.50

subject

Has Abstract

pub_date

1983-05-01 00:00:00

pages

50-5

issue

1

eissn

0032-0889

issn

1532-2548

journal_volume

72

pub_type

杂志文章
  • Phytochrome Regulation of the Response to Exogenous Gibberellins by Epicotyls of Vigna sinensis.

    abstract::The elongation rate of cowpea epicotyls from whole cowpea (Vigna sinensis) seedlings and derooted and debladed plants (explants) increased after the main light period (8-hour duration) was extended with either continuous low intensity tungsten light or brief (5 minutes) far-red (FR) irradiation. This end-of-day FR eff...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.85.1.212

    authors: García-Martínez JL,Keith B,Bonner BA,Stafford AE,Rappaport L

    更新日期:1987-09-01 00:00:00

  • Multiple Integrated Root Phenotypes Are Associated with Improved Drought Tolerance.

    abstract::To test the hypothesis that multiple integrated root phenotypes would co-optimize drought tolerance, we phenotyped the root anatomy and architecture of 400 mature maize (Zea mays) genotypes under well-watered and water-stressed conditions in the field. We found substantial variation in all 23 root phenes measured. A p...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.20.00211

    authors: Klein SP,Schneider HM,Perkins AC,Brown KM,Lynch JP

    更新日期:2020-07-01 00:00:00

  • Chorismate Mutase Isoenzymes from Selected Plants and Their Immunological Comparison with the Isoenzymes from Sorghum bicolor.

    abstract::The isoenzyme pattern of chorismate mutase (EC 5.4.99.5) was examined by diethylaminoethyl-cellulose chromatography in a wide variety of plants. All plants contained a regulated form of chorismate mutase (CM-1), and most contained an additional, unregulated form (CM-2). The regulatory properties of CM-1 differed signi...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.81.3.717

    authors: Singh BK,Lonergan SG,Conn EE

    更新日期:1986-07-01 00:00:00

  • Tissue Slice and Particulate beta-Glucan Synthetase Activities from Pisum Epicotyls.

    abstract::beta-Glucan synthetase activity in growing regions of pea (Pisum sativum L.) epicotyls was assayed by supplying UDP-glucose to particulate fractions of tissue homogenates or to thin tissue slices. Particulate fractions are less active in forming alkali-insoluble glucan than slices from the same tissue, although many k...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.61.6.938

    authors: Raymond Y,Fincher GB,Maclachlan GA

    更新日期:1978-06-01 00:00:00

  • Nitrogen recycling and remobilization are differentially controlled by leaf senescence and development stage in Arabidopsis under low nitrogen nutrition.

    abstract::Five recombinant inbred lines (RILs) of Arabidopsis (Arabidopsis thaliana), previously selected from the Bay-0 x Shahdara RIL population on the basis of differential leaf senescence phenotypes (from early senescing to late senescing) when cultivated under nitrogen (N)-limiting conditions, were analyzed to monitor meta...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.108.119040

    authors: Diaz C,Lemaître T,Christ A,Azzopardi M,Kato Y,Sato F,Morot-Gaudry JF,Le Dily F,Masclaux-Daubresse C

    更新日期:2008-07-01 00:00:00

  • Compartmentation in Vicia faba Leaves: I. Kinetics of C in the Tissues following Pulse Labeling.

    abstract::Leaflets of Vicia faba were pulse-labeled with (14)CO(2) to follow the subsequent movement of photosynthate between leaf tissues. Samples were taken during a (12)CO(2) chase, quick frozen, freeze-substituted, and embedded in methacrylate. Paradermal sections provided tissue samples consisting only of upper epidermis, ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.55.4.699

    authors: Outlaw WH,Fisher DB

    更新日期:1975-04-01 00:00:00

  • alpha-Amylase Isozymes in Gibberellic Acid-treated Barley Half-seeds.

    abstract::The presence of multiple forms of alpha-amylase in gibberellic acid-treated embryoless barley half-seeds was demonstrated by separation on diethylaminoethyl-Sephadex and isoelectric focusing polyacrylamide gel disc electrophoresis. Two major alpha-amylase fractions (A and B), each consisting of two to three isozyme co...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.46.4.586

    authors: Tanaka Y,Akazawa T

    更新日期:1970-10-01 00:00:00

  • Molecular and biochemical characterization of the selenocysteine Se-methyltransferase gene and Se-methylselenocysteine synthesis in broccoli.

    abstract::Selenium (Se) plays an indispensable role in human nutrition and has been implicated to have important health benefits, including being a cancer preventative agent. While different forms of Se vary in their anticarcinogenic efficacy, Se-methylselenocysteine (SeMSC) has been demonstrated to be one of the most effective...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.104.056549

    authors: Lyi SM,Heller LI,Rutzke M,Welch RM,Kochian LV,Li L

    更新日期:2005-05-01 00:00:00

  • Cytochrome c is released in a reactive oxygen species-dependent manner and is degraded via caspase-like proteases in tobacco Bright-Yellow 2 cells en route to heat shock-induced cell death.

    abstract::To gain some insight into the mechanism of plant programmed cell death, certain features of cytochrome c (cyt c) release were investigated in heat-shocked tobacco (Nicotiana tabacum) Bright-Yellow 2 cells in the 2- to 6-h time range. We found that 2 h after heat shock, cyt c is released from intact mitochondria into t...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.106.078683

    authors: Vacca RA,Valenti D,Bobba A,Merafina RS,Passarella S,Marra E

    更新日期:2006-05-01 00:00:00

  • Western blot analysis of cereal grain prolamins using an antibody to carboxyl-linked indoleacetic Acid.

    abstract::A monoclonal antibody raised against carboxyl-linked IAA was used in Western blot analysis of storage proteins from kernels of Avena sativa, Pennisetum americanum, Sorghum bicolor, and Zea mays. IAA or an IAA-like molecule is associated with the ethanolsoluble protein fraction of the seed. Western blotting of commerci...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.96.4.1076

    authors: Leverone LA,Stroup TL,Caruso JL

    更新日期:1991-08-01 00:00:00

  • Analysis of cell division and elongation underlying the developmental acceleration of root growth in Arabidopsis thaliana.

    abstract::To investigate the relation between cell division and expansion in the regulation of organ growth rate, we used Arabidopsis thaliana primary roots grown vertically at 20 degreesC with an elongation rate that increased steadily during the first 14 d after germination. We measured spatial profiles of longitudinal veloci...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.116.4.1515

    authors: Beemster GT,Baskin TI

    更新日期:1998-04-01 00:00:00

  • Further characterization of ribosome binding to thylakoid membranes.

    abstract::Previous work indicated more polysomes bound to pea (Pisum sativum cv Progress No. 9) thylakoids in light than in the dark, in vivo (LE Fish, AT Jagendorf 1982 Plant Physiol 69: 814-825). With isolated intact chloroplasts incubated in darkness, addition of MgATP had no effect but 24 to 74% more RNA was thylakoid-bound...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.84.1.31

    authors: Hurewitz J,Jagendorf AT

    更新日期:1987-05-01 00:00:00

  • Free amino Acid composition of leaf exudates and Phloem sap : a comparative study in oats and barley.

    abstract::Comparisons were made between the free amino acid composition in leaf exudates and that in pure phloem sap, using twin samples taken from a single leaf of two oat (Avena sativa L.) and three barley (Hordeum vulgare L.) varieties. Leaf exudate was collected in a 5 mm EDTA-solution (pH 7.0) from cut leaf blades and phlo...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.92.1.222

    authors: Weibull J,Ronquist F,Brishammar S

    更新日期:1990-01-01 00:00:00

  • Identification of AtNDI1, an internal non-phosphorylating NAD(P)H dehydrogenase in Arabidopsis mitochondria.

    abstract::Plant mitochondria contain non-phosphorylating NAD(P)H dehydrogenases (DHs) that are not found in animal mitochondria. The physiological function, substrate specificity, and location of enzymes within this family have yet to be conclusively determined. We have linked genome sequence information to protein and biochemi...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.103.029363

    authors: Moore CS,Cook-Johnson RJ,Rudhe C,Whelan J,Day DA,Wiskich JT,Soole KL

    更新日期:2003-12-01 00:00:00

  • Sulfur transfer through an arbuscular mycorrhiza.

    abstract::Despite the importance of sulfur (S) for plant nutrition, the role of the arbuscular mycorrhizal (AM) symbiosis in S uptake has received little attention. To address this issue, 35S-labeling experiments were performed on mycorrhizas of transformed carrot (Daucus carota) roots and Glomus intraradices grown monoxenicall...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.108.129866

    authors: Allen JW,Shachar-Hill Y

    更新日期:2009-01-01 00:00:00

  • Potassium Fluxes in Chlamydomonas reinhardtii (II. Compartmental Analysis).

    abstract::42K+ and 86Rb+ were used to determine the subcellular distribution of potassium in Chlamydomonas reinhardtii by compartmental analysis. In both wild type and a mutant strain, three distinct compartments (referred to as I, II, and III) were apparent. Using 42K+, we found that these had half-lives for K+ exchange of 1.0...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.108.4.1537

    authors: Malhotra B,Glass A

    更新日期:1995-08-01 00:00:00

  • Cesium toxicity in Arabidopsis.

    abstract::Cesium (Cs) is chemically similar to potassium (K). However, although K is an essential element, Cs is toxic to plants. Two contrasting hypotheses to explain Cs toxicity have been proposed: (1) extracellular Cs+ prevents K+ uptake and, thereby, induces K starvation; and (2) intracellular Cs+ interacts with vital K(+)-...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.104.046672

    authors: Hampton CR,Bowen HC,Broadley MR,Hammond JP,Mead A,Payne KA,Pritchard J,White PJ

    更新日期:2004-11-01 00:00:00

  • Identification of a new glucosinolate-rich cell type in Arabidopsis flower stalk.

    abstract::Distribution of K, Ca, Cl, S, and P in freeze-dried sections of Arabidopsis flower stalk was analyzed by energy dispersive x-ray imaging. Concentrations of these elements in different cell types were quantified by microanalysis of single-cell samples and phloem exudates. Results showed a differential pattern of distri...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.124.2.599

    authors: Koroleva OA,Davies A,Deeken R,Thorpe MR,Tomos AD,Hedrich R

    更新日期:2000-10-01 00:00:00

  • Iron deficiency-induced secretion of phenolics facilitates the reutilization of root apoplastic iron in red clover.

    abstract::Phenolic compounds are frequently reported to be the main components of root exudates in response to iron (Fe) deficiency in Strategy I plants, but relatively little is known about their function. Here, we show that removal of secreted phenolics from the root-bathing solution almost completely inhibited the reutilizat...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.107.095794

    authors: Jin CW,You GY,He YF,Tang C,Wu P,Zheng SJ

    更新日期:2007-05-01 00:00:00

  • Combined low temperature-high light effects on gas exchange properties of jojoba leaves.

    abstract::Jojoba (Simmondsia chinensis [Link] Schneider) is an important crop in desert climates. A relatively high frequency of periods of chilling and high photon flux density (PFD) in this environment makes photoinhibition likely, resulting in a reduction of assimilation capacity in overwintering leaves. This could explain t...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.91.4.1580

    authors: Loreto F,Bongi G

    更新日期:1989-12-01 00:00:00

  • Plasma membrane H+-ATPase is involved in auxin-mediated cell elongation during wheat embryo development.

    abstract::Previous investigations suggested that specific auxin spatial distribution due to auxin movements to particular embryonic regions was important for normal embryonic pattern formation. To gain information on the molecular mechanism(s) by which auxin acts to direct pattern formation in specific embryonic regions, the ro...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.013466

    authors: Rober-Kleber N,Albrechtová JT,Fleig S,Huck N,Michalke W,Wagner E,Speth V,Neuhaus G,Fischer-Iglesias C

    更新日期:2003-03-01 00:00:00

  • Changes in transcript abundance in Chlamydomonas reinhardtii following nitrogen deprivation predict diversion of metabolism.

    abstract::Like many microalgae, Chlamydomonas reinhardtii forms lipid droplets rich in triacylglycerols when nutrient deprived. To begin studying the mechanisms underlying this process, nitrogen (N) deprivation was used to induce triacylglycerol accumulation and changes in developmental programs such as gametogenesis. Comparati...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.110.165159

    authors: Miller R,Wu G,Deshpande RR,Vieler A,Gärtner K,Li X,Moellering ER,Zäuner S,Cornish AJ,Liu B,Bullard B,Sears BB,Kuo MH,Hegg EL,Shachar-Hill Y,Shiu SH,Benning C

    更新日期:2010-12-01 00:00:00

  • Photosynthesis, leaf resistances, and ribulose-1,5-bisphosphate carboxylase degradation in senescing barley leaves.

    abstract::The relationship between loss of ribulose-1,5-bisphosphate carboxylase (RuBPCase) and the decline in photosynthesis during the senescence of barley primary leaves was assessed. Loss of RuBPCase accounted for about 85% of the decrease in soluble protein. RuBPCase was highly correlated with in vitro RuBPCase activity (r...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.65.6.1103

    authors: Friedrich JW,Huffaker RC

    更新日期:1980-06-01 00:00:00

  • The occurrence of phytoferritin and its relationship to effectiveness of soybean nodules.

    abstract::Polyacrylamide gel electrophoresis and electron microscopy revealed that accumulation of iron-protein in soybean nodules is influenced by nodule age, mutation in bradyrhizobia, and rhizobial/bradyrhizobial strain-soybean cultivar interactions. Iron-protein concentrations (micrograms per milligram protein) were inverse...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.83.2.299

    authors: Ko MP,Huang PY,Huang JS,Barker KR

    更新日期:1987-02-01 00:00:00

  • Developmental and light-regulated expression of individual members of the light-harvesting complex b gene family in Pinus palustris.

    abstract::Angiosperms requires light for multiple aspects of chloroplast development, including chlorophyll synthesis and induction of expression of the mRNAs encoding the major polypeptides of the light-harvesting complex of photosystem II (Lhcb genes). In contrast, many conifers, including pines, firs, and spruces, can accumu...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.111.2.627

    authors: Peer W,Silverthorne J,Peters JL

    更新日期:1996-06-01 00:00:00

  • A circuit analog model for studying quantitative water relations of plant tissues.

    abstract::Using arrays of resistors and capacitors, a lumped circuit analog of plant tissue is developed. The circuit elements of the analog are identified in terms of physiological variables (hydraulic conductivities, water capacities, and cell dimensions) which can be measured in the laboratory. With the aid of a circuit simu...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.64.5.712

    authors: Molz FJ,Kerns DV,Peterson CM,Dane JH

    更新日期:1979-11-01 00:00:00

  • Stomatal responses to flooding of the intercellular air spaces suggest a vapor-phase signal between the mesophyll and the guard cells.

    abstract::Flooding the intercellular air spaces of leaves with water was shown to cause rapid closure of stomata in Tradescantia pallida, Lactuca serriola, Helianthus annuus, and Oenothera caespitosa. The response occurred when water was injected into the intercellular spaces, vacuum infiltrated into the intercellular spaces, o...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.110.157685

    authors: Sibbernsen E,Mott KA

    更新日期:2010-07-01 00:00:00

  • Enzymic Analysis of Feruloylated Arabinoxylans (Feraxan) Derived from Zea mays Cell Walls I : Purification of Novel Enzymes Capable of Dissociating Feraxan Fragments from Zea mays Coleoptile Cell Wall.

    abstract::Three novel beta-xylan xylanohydrolases capable of dissociating ferulated arabinoxylan (Feraxan) from maize (Zea mays L. hybrid B73 x Mo17) coleoptile sections and two conventional beta-xylan xylanohydrolases (xylanases) were purified from a Bacillus subtilis industrial enzyme preparation (Novo Ban L-120). The Feraxan...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.87.4.883

    authors: Nishitani K,Nevins DJ

    更新日期:1988-08-01 00:00:00

  • Purification and Characterization of the Pea Chloroplast Pyruvate Dehydrogenase Complex : A Source of Acetyl-CoA and NADH for Fatty Acid Biosynthesis.

    abstract::The pyruvate dehydrogenase complex has been purified 76-fold, to a specific activity of 0.6 mumoles per minute per milligram protein, beginning with isolated pea (Pisum sativum L. var Little Marvel) chloroplasts. Purification was accomplished by rate zonal sedimentation, polyethyleneglycol precipitation, and ethyl-aga...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.77.3.571

    authors: Camp PJ,Randall DD

    更新日期:1985-03-01 00:00:00

  • Effects of gibberellic Acid and gold light on germination, enzyme activities, and amino Acid pool size in a dwarf strain of watermelon.

    abstract::Gibberellic acid (GA(3)) promotes and continuous gold light inhibits germination of seeds of a dwarf strain (WB-2) of watermelon [Citrullus lanatus (Thunb.) Matsu. and Nakai]. Osmotic inhibition of germination with mannitol in light-grown seeds of WB-2 was only slightly reversed by GA(3) at the concentrations used, wh...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.62.1.6

    authors: Evensen KB,Loy JB

    更新日期:1978-07-01 00:00:00