Tissue Slice and Particulate beta-Glucan Synthetase Activities from Pisum Epicotyls.

Abstract:

:beta-Glucan synthetase activity in growing regions of pea (Pisum sativum L.) epicotyls was assayed by supplying UDP-glucose to particulate fractions of tissue homogenates or to thin tissue slices. Particulate fractions are less active in forming alkali-insoluble glucan than slices from the same tissue, although many kinetic characteristics (pH and Mg(2+) optimum, apparent K(m)) are similar for the two systems. Synthesis by tissue slices progresses linearly without lag period for at least an hour and is proportional to cut surface area. It is much more rapid from UDP-glucose than from glucose, glucose-1-P, or sucrose. Tests with plasmolyzing agents and trypsin support the conclusion that synthesis from UDP-glucose by slices occurs at accessible surfaces of cut cells. Analyses of glucan products by GLC of partially methylated and acetylated derivatives and by hydrolysis with various beta-glucanases all show that both beta-1,3 and beta-1,4 linkages are formed by particulate fractions and slices at substrate concentrations ranging from micro- to millimolar. beta-1,4 Linkages predominate at low substrate (5 mum) concentration. Kinetic data indicate that the capacity to synthesize beta-1,3-glucan is substrate-activated, and this product predominates in preparations supplied with high (5 mm) substrate.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Raymond Y,Fincher GB,Maclachlan GA

doi

10.1104/pp.61.6.938

subject

Has Abstract

pub_date

1978-06-01 00:00:00

pages

938-42

issue

6

eissn

0032-0889

issn

1532-2548

journal_volume

61

pub_type

杂志文章
  • Induction and Regulation of Heat-Shock Gene Expression by an Amino Acid Analog in Soybean Seedlings.

    abstract::The effect of the proline analog azetidine-2-carboxylic acid (Aze) on the induction and the regulation of heat-shock (HS) mRNA accumulation and heat-shock protein (HSP) synthesis in soybean (Glycine max) seedlings was studied. Treatment with Aze elicited an HS-like response at the normal growth temperature, 28[deg]C, ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.110.1.241

    authors: Lee Y,Nagao RT,Lin CY,Key JL

    更新日期:1996-01-01 00:00:00

  • An investigation of the mineral content of barley grains and seedlings.

    abstract::The Ca, Mg, K, and P content of dry barley (Hordeum vulgare) grains and seedlings was investigated using energy dispersive x-ray analysis and neutron activation analysis. Energy dispersive x-ray analysis of protein bodies in aleurone cells showed that these bodies contained very little Ca in relation to P, Mg, and K. ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.86.1.93

    authors: Stewart A,Nield H,Lott JN

    更新日期:1988-01-01 00:00:00

  • Genetic Regulation of Development in Sorghum bicolor: II. Effect of the ma(3) Allele Mimicked by GA(3).

    abstract::The class III, ma(3) (R)-containing maturity genotypes of Sorghum bicolor (L.) Moench differ from all of the other maturity genotypes in that they flower earlier, exhibit rapid shoot elongation sooner after floral differentiation, and exhibit several morphological differences. In the present study, a class III, ma(3) ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.82.2.581

    authors: Pao CI,Morgan PW

    更新日期:1986-10-01 00:00:00

  • H-ATPase Activity from Storage Tissue of Beta vulgaris: IV. N,N'-Dicyclohexylcarbodiimide Binding and Inhibition of the Plasma Membrane H-ATPase.

    abstract::The molecular weight and isoelectric point of the plasma membrane H(+)-ATPase from red beet storage tissue were determined using N,N'-dicyclohexylcarbodiimide (DCCD) and a H(+)-ATPase antibody. When plasma membrane vesicles were incubated with 20 micromolar [(14)C]-DCCD at 0 degrees C, a single 97,000 dalton protein w...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.83.3.569

    authors: Oleski NA,Bennett AB

    更新日期:1987-03-01 00:00:00

  • Characterization of the Arrest in Anther Development Associated with Gibberellin Deficiency of the gib-1 Mutant of Tomato.

    abstract::The role of gibberellins in flower bud development was investigated by studying the gib-1 mutant of tomato, Lycopersicon esculentum. This gibberellin-deficient mutant initiates flower buds, but floral development is not completed unless the mutant is treated with gibberellin. Treatment with other plant growth regulato...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.97.1.409

    authors: Jacobsen SE,Olszewski NE

    更新日期:1991-09-01 00:00:00

  • State 1-State 2 Transitions in a Unicellular Green Algae : Analysis of In Vivo Chlorophyll Fluorescence Induction Curves in the Presence of 3-(3,4-Dichlorophenyl)-1, 1-dimethylurea (DCMU).

    abstract::A study has been made on the State 1-State 2 transitions exhibited by the unicellular green algae Chlorella pyrenoidosa. Chlorophyll fluorescence induction curves from algae adapted to State 1 or State 2 have been analyzed and a comparison made with similar curves produced by decreasing the intensity of light going to...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.72.4.1119

    authors: Hodges M,Barber J

    更新日期:1983-08-01 00:00:00

  • Subcellular localization and membrane topology of the melon ethylene receptor CmERS1.

    abstract::Ethylene receptors are multispanning membrane proteins that negatively regulate ethylene responses via the formation of a signaling complex with downstream elements. To better understand their biochemical functions, we investigated the membrane topology and subcellular localization of CmERS1, a melon (Cucumis melo) et...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.106.080523

    authors: Ma B,Cui ML,Sun HJ,Takada K,Mori H,Kamada H,Ezura H

    更新日期:2006-06-01 00:00:00

  • Fusicoccin binding to its plasma membrane receptor and the activation of the plasma membrane H(+)-ATPase. IV. Fusicoccin induces the association between the plasma membrane H(+)-ATPase and the fusicoccin receptor.

    abstract::Different approaches were utilized to investigate the mechanism by which fusicoccin (FC) induces the activation of the H(+)-ATPase in plasma membrane (PM) isolated from radish (Raphanus sativus L.) seedlings treated in vivo with (FC-PM) or without (C-PM) FC. Treatment of FC-PM with different detergents indicated that ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.116.2.529

    authors: Olivari C,Meanti C,De Michelis MI,Rasi-Caldogno F

    更新日期:1998-02-01 00:00:00

  • Modulation of PSI and PSII Organization During Loss and Repair of Photosynthetic Activity in a Temperature Sensitive Mutant of Chlorella pyrenoidosa.

    abstract::Photosynthetic activity and organization of chlorophyll(Chl)-protein complexes in a temperature sensitive mutant of Chlorella pyrenoidosa have been investigated. The mutant is practically indistinguishable from wild type cells when grown at 25 C. However, mutant cells grown at 33 C do not synthesize Chl and lose their...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.68.6.1264

    authors: Lavintman N,Galling G,Ohad I

    更新日期:1981-12-01 00:00:00

  • Cell-Wall Proteins Induced by Water Deficit in Bean (Phaseolus vulgaris L.) Seedlings.

    abstract::In the last few years, much attention has been given to the role of proteins that accumulate during water deficit. In this work, we analyzed the electrophoretic patterns of basic protein extracts, enriched for a number of cell-wall proteins, from bean (Phaseolus vulgaris L.) seedlings and 21-d-old plants subjected to ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.107.4.1119

    authors: Covarrubias AA,Ayala JW,Reyes JL,Hernandez M,Garciarrubio A

    更新日期:1995-04-01 00:00:00

  • Abscisic Acid and cutout in cotton.

    abstract::A decline in growth, flowering, and boll (fruit) retention is referred to as cutout in cotton (Gossypium hirsutum L.). Fruit load affects cutout, possibly through hormonal effects. Experiments were conducted to test the hypothesis that fruits are a source of abscisic acid (ABA) that moves into fruiting branches and gr...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.77.1.16

    authors: Guinn G

    更新日期:1985-01-01 00:00:00

  • First evidence for polyamine conjugation mediated by an enzymic activity in plants.

    abstract::An enzyme activity, found for the first time in plants, mainly located in the 22,000g supernatant of the crude extract of sprout apices of Helianthus tuberosus L. cv OB1 tubers, is able in vitro to covalently bind polyamines to endogenous substrates of different molecular weights. The major assay parameters, such as p...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.87.3.757

    authors: Serafini-Fracassini D,Del Duca S,D'Orazi D

    更新日期:1988-07-01 00:00:00

  • Gibberellins promote trichome formation by Up-regulating GLABROUS1 in arabidopsis

    abstract::Trichome development is dependent on gibberellin (GA) signaling in Arabidopsis thaliana. Using the GA-deficient mutant ga1-3, the GA-response mutant spy-5, and uniconazol (a GA-biosynthesis inhibitor), we show that the GA level response correlates positively with both trichome number and trichome branch number. Two ge...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.117.2.375

    authors: Perazza D,Vachon G,Herzog M

    更新日期:1998-06-01 00:00:00

  • An evaluation of 2,5-norbornadiene as a reversible inhibitor of ethylene action in deepwater rice.

    abstract::Partial submergence of deepwater rice (Oryza sativa L. cv Habiganj Aman II) elicits three responses: enhancement of internodal elongation, inhibition of leaf growth, and promotion of adventitious root formation. All three responses can be induced in isolated stem sections by treatment with ethylene. Dose-response curv...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.84.2.395

    authors: Bleecker AB,Rose-John S,Kende H

    更新日期:1987-06-01 00:00:00

  • Phosphate Starvation Alters Abiotic-Stress-Induced Cytosolic Free Calcium Increases in Roots.

    abstract::Phosphate (Pi) deficiency strongly limits plant growth, and plant roots foraging the soil for nutrients need to adapt to optimize Pi uptake. Ca2+ is known to signal in root development and adaptation but has to be tightly controlled, as it is highly toxic to Pi metabolism. Under Pi starvation and the resulting decreas...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.18.01469

    authors: Matthus E,Wilkins KA,Swarbreck SM,Doddrell NH,Doccula FG,Costa A,Davies JM

    更新日期:2019-04-01 00:00:00

  • Molecular identification of an Arabidopsis S-adenosylmethionine transporter. Analysis of organ distribution, bacterial expression, reconstitution into liposomes, and functional characterization.

    abstract::Despite much study of the role of S-adenosylmethionine (SAM) in the methylation of DNA, RNA, and proteins, and as a cofactor for a wide range of biosynthetic processes, little is known concerning the intracellular transport of this essential metabolite. Screening of the Arabidopsis (Arabidopsis thaliana) genome yielde...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.106.086975

    authors: Palmieri L,Arrigoni R,Blanco E,Carrari F,Zanor MI,Studart-Guimaraes C,Fernie AR,Palmieri F

    更新日期:2006-11-01 00:00:00

  • Trichomes of tobacco excrete zinc as zinc-substituted calcium carbonate and other zinc-containing compounds.

    abstract::Tobacco (Nicotiana tabacum L. cv Xanthi) plants were exposed to toxic levels of zinc (Zn). Zn exposure resulted in toxicity signs in plants, and these damages were partly reduced by a calcium (Ca) supplement. Confocal imaging of intracellular Zn using Zinquin showed that Zn was preferentially accumulated in trichomes....

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.106.082743

    authors: Sarret G,Harada E,Choi YE,Isaure MP,Geoffroy N,Fakra S,Marcus MA,Birschwilks M,Clemens S,Manceau A

    更新日期:2006-07-01 00:00:00

  • Water relationships and incorporation of C assimilates in tubers of potato plants differing in potassium nutrition.

    abstract::Potato (Solanum tuberosum L. cv Saturna) plants were grown in pots with varying supplies of K fertilizer (1.25, 5, and 10 grams K(2)O per 12 kilograms soil). Four weeks after midflower, plants were supplied with (14)CO(2) for 12 hours and osmotic and water potential in tubers were determined. Assimilation of (14)CO(2)...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.73.4.956

    authors: Beringer H,Haeder HE,Lindhauer M

    更新日期:1983-12-01 00:00:00

  • Identification of quantitative trait loci controlling gene expression during the innate immunity response of soybean.

    abstract::Microbe-associated molecular pattern-triggered immunity (MTI) is an important component of the plant innate immunity response to invading pathogens. However, most of our knowledge of MTI comes from studies of model systems with relatively little work done with crop plants. In this work, we report on variation in both ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.111.183327

    authors: Valdés-López O,Thibivilliers S,Qiu J,Xu WW,Nguyen TH,Libault M,Le BH,Goldberg RB,Hill CB,Hartman GL,Diers B,Stacey G

    更新日期:2011-12-01 00:00:00

  • Manipulating broad-spectrum disease resistance by suppressing pathogen-induced auxin accumulation in rice.

    abstract::Breeding crops with the quality of broad-spectrum disease resistance using genetic resources is one of the principal goals of crop improvement. However, the molecular mechanism of broad-spectrum resistance remains largely unknown. Here, we show that GH3-2, encoding an indole-3-acetic acid (IAA)-amido synthetase, media...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.110.163774

    authors: Fu J,Liu H,Li Y,Yu H,Li X,Xiao J,Wang S

    更新日期:2011-01-01 00:00:00

  • Biochemical factors affecting protein accumulation in the rice grain.

    abstract::Rice grains (Oryza sativa L.) from three varieties and three pairs of lines with different protein content were collected at 4-day intervals from 4 to 32 days after flowering. The samples were analyzed for protein, free amino nitrogen, ribonucleic acid, protease activity, and ribonuclease activity. In addition, the ca...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.46.5.743

    authors: Cruz LJ,Cagampang GB,Juliano BO

    更新日期:1970-11-01 00:00:00

  • An Explanation for the Difference in Photosynthetic Capabilities of Healthy and Beet Yellows Virus-infected Sugar Beets (Beta vulgaris L.).

    abstract::Sugar beets (Beta vulgaris L.) infected with the Beet Yellows Virus exhibit lower rates of net photosynthesis at light saturation than do healthy plants. These Pn reductions were correlated with increases in leaf resistance to water vapor loss. Theoretical analyses demonstrated that, although the leaf resistance to wa...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.50.5.576

    authors: Hall AE,Loomis RS

    更新日期:1972-11-01 00:00:00

  • Soybean root suberin: anatomical distribution, chemical composition, and relationship to partial resistance to Phytophthora sojae.

    abstract::Soybean (Glycine max L. Merr.) is a versatile and important agronomic crop grown worldwide. Each year millions of dollars of potential yield revenues are lost due to a root rot disease caused by the oomycete Phytophthora sojae (Kaufmann & Gerdemann). Since the root is the primary site of infection by this organism, we...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.106.091090

    authors: Thomas R,Fang X,Ranathunge K,Anderson TR,Peterson CA,Bernards MA

    更新日期:2007-05-01 00:00:00

  • Effects of temperature on orthophosphate absorption by excised corn roots.

    abstract::The uptake of orthophosphate ((32)P) by excised corn roots, Zea mays L. was studied using roots grown on 0.2 mm CaSO(4). Nine concentrations of KH(2)PO(4) from 1 to 256 mum were used at temperatures of 20 degrees , 30 degrees , and 40 degrees . Enzyme kinetic analysis was applied to the data obtained. Two apparent mec...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.42.10.1407

    authors: Carter OG,Lathwell DJ

    更新日期:1967-10-01 00:00:00

  • Pyrophosphate Fructose-6-P 1-Phosphotransferase from Tomato Fruit : Evidence for Change during Ripening.

    abstract::Three forms of pyrophosphate fructose-6-phosphate 1-phosphotransferase (PFP) were purified from both green and red tomato (Lycopersicon esculentum) fruit: (a) a classical form (designated Q(2)) containing alpha- (66 kilodalton) and beta- (60 kilodalton) subunits; (b) a form (Q(1)) containing a beta-doublet subunit; an...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.94.2.499

    authors: Wong JH,Kiss F,Wu MX,Buchanan BB

    更新日期:1990-10-01 00:00:00

  • Catalytic activity of maize leaf phosphoenolpyruvate carboxylase in relation to oligomerization.

    abstract::The relationship between the state of oligomerization and activity of purified maize leaf phosphoenolpyruvate carboxylase using size exclusion high performance liquid chromatography was examined. Maximum activities of 35 to 38 micromoles per minute per milligram protein were found when 100% of the enzyme was in its te...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.80.4.848

    authors: Walker GH,Ku MS,Edwards GE

    更新日期:1986-04-01 00:00:00

  • pTAC10, a Key Subunit of Plastid-Encoded RNA Polymerase, Promotes Chloroplast Development.

    abstract::Regulation of photosynthetic gene expression by plastid-encoded RNA polymerase (PEP) is essential for chloroplast development. The activity of PEP largely relies on at least 12 PEP-associated proteins (PAPs) encoded in the nuclear genome of plant cells. A recent model proposed that these PAPs regulate the establishmen...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.17.00248

    authors: Chang SH,Lee S,Um TY,Kim JK,Do Choi Y,Jang G

    更新日期:2017-05-01 00:00:00

  • Role of indoleacetic Acid and abscisic Acid in the correlative control by fruits of axillary bud development and leaf senescence.

    abstract::When fully filled pods of bean plants were deseeded, the rate of axillary bud growth and the chlorophyll content of leaves were increased. Application of 0.1% indoleacetic acid (IAA) in lanolin on the deseeded pods caused abscission of axillary buds, inhibited growth of the remaining buds, and decreased leaf chlorophy...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.68.2.476

    authors: Tamas IA,Engels CJ

    更新日期:1981-08-01 00:00:00

  • Proteolytic Activity in Soybean Root Nodules : Activity in Host Cell Cytosol and Bacteroids throughout Physiological Development and Senescence.

    abstract::Root nodules were harvested from chamber-grown soybean (Glycine max L. Merrill cv Woodworth) plants throughout development. Apparent nitrogenase activity (acetylene reduction) peaked before seeds began to develop, but a significant amount of activity remained as the seeds matured. Nodule senescence was defined as the ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.71.4.797

    authors: Pfeiffer NE,Torres CM,Wagner FW

    更新日期:1983-04-01 00:00:00

  • Oxidative Stress Induces Partial Degradation of the Large Subunit of Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase in Isolated Chloroplasts of Barley.

    abstract::The effects of oxidative stress on the degradation of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco; EC 4.1.1.39) were studied in isolated chloroplasts from barley (Hordeum vulgare L. cv Angora). Active oxygen (AO) was generated by varying the light intensity, the oxygen concentration, or the addition of he...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.111.3.789

    authors: Desimone M,Henke A,Wagner E

    更新日期:1996-07-01 00:00:00