Compartmentation in Vicia faba Leaves: I. Kinetics of C in the Tissues following Pulse Labeling.

Abstract:

:Leaflets of Vicia faba were pulse-labeled with (14)CO(2) to follow the subsequent movement of photosynthate between leaf tissues. Samples were taken during a (12)CO(2) chase, quick frozen, freeze-substituted, and embedded in methacrylate. Paradermal sections provided tissue samples consisting only of upper epidermis, palisade parenchyma, spongy parenchyma and veins, spongy parenchyma, or lower epidermis. Most CO(2) fixation occurred in the palisade parenchyma, but its (14)C content declined rapidly after labeling. Concomitant with the decline of activity in the palisade parenchyma, there was an increase in activity in the spongy parenchyma and upper epidermis and a slow increase in the lower epidermis. Activity in the palisade parenchyma and spongy parenchyma eventually reached similar levels and remained constant. Tissue samples containing veins were consistently the most radioactive, and activity in those samples showed a decline. Very little change occurred in the insoluble fraction from any tissue. The results support previous assumptions regarding the pathway of assimilate transport to the veins, and demonstrate the rapidity of such transport. Sucrose is apparently the principal mobile compound.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Outlaw WH,Fisher DB

doi

10.1104/pp.55.4.699

subject

Has Abstract

pub_date

1975-04-01 00:00:00

pages

699-703

issue

4

eissn

0032-0889

issn

1532-2548

journal_volume

55

pub_type

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