A fast and easy technique for the isolation of aleurone layers.

Abstract:

:Although the aleurone layer of ceral grain seeds has many advantages for the study of gibberellin action, it has the disadvantage that the hand-isolation of the aleurone layers is time-consuming. To overcome this disadvantage, a commercially available pasta machine was modified and used to remove aleurone layers from imbibed barley (Hordeum vulgare) seeds. This equipment allows isolation of a thousand layers in 5 minutes compared to the 3 to 4 hours required to hand-isolate them. The machine-made aleurone layers are gibberellic-acid responsive and the response is similar both qualitatively and quantitatively to that of hand-isolated layers.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Murthy PP

doi

10.1104/pp.90.2.388

subject

Has Abstract

pub_date

1989-06-01 00:00:00

pages

388-9

issue

2

eissn

0032-0889

issn

1532-2548

journal_volume

90

pub_type

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