N and C NMR determination of methionine metabolism in developing soybean cotyledons.

Abstract:

:The metabolism of d- and l-methionine by immature cotyledons of soybean (Glycine max, L. cv Elf) grown in culture has been investigated using solid-state (13)C and (15)N nuclear magnetic resonance. d-Methionine is taken up by the cotyledons and converted to an amide, most likely by N-malonylation. About 16% of the l-methionine taken up is incorporated intact into protein, and 25% remains as soluble methionine. Almost two-thirds of the l-methionine that enters the cotyledons is degraded. The largest percentage of this is used in transmethylation of the carboxyl groups of pectin. Methionine is not extensively converted to polyamines. We attribute the stimulation of growth of the cotyledons by exogenous methionine to the bypassing of a rate-limiting methyl-transfer step in the synthesis of methionine itself, and subsequently of pectins and proteins.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Coker GT,Garbow JR,Schaefer J

doi

10.1104/pp.83.3.698

subject

Has Abstract

pub_date

1987-03-01 00:00:00

pages

698-702

issue

3

eissn

0032-0889

issn

1532-2548

journal_volume

83

pub_type

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