Abstract:
:Dynamin and dynamin-like proteins are GTP-binding proteins involved in vesicle trafficking. In soybean, a 68-kD dynamin-like protein called phragmoplastin has been shown to be associated with the cell plate in dividing cells (Gu and Verma, 1996). Five ADL1 genes encoding dynamin-like proteins related to phragmoplastin have been identified in the completed Arabidopsis genome. Here we report that ADL1Ap is associated with punctate subcellular structures and with the cell plate in dividing cells. To assess the function of ADL1Ap we utilized a reverse genetic approach to isolate three separate Arabidopsis mutant lines containing T-DNA insertions in ADL1A. Homozygous adl1A seeds were shriveled and mutant seedlings arrested soon after germination, producing only two leaf primordia and severely stunted roots. Immunoblotting revealed that ADL1Ap expression was not detectable in the mutants. Despite the loss of ADL1Ap, the mutants did not display any defects in cytokinesis, and growth of the mutant seedlings could be rescued in tissue culture by the addition of sucrose. Although these sucrose-rescued plants displayed normal vegetative growth and flowered, they set very few seeds. Thus, ADL1Ap is critical for several stages of plant development, including embryogenesis, seedling development, and reproduction. We discuss the putative role of ADL1Ap in vesicular trafficking, cytokinesis, and other aspects of plant growth.
journal_name
Plant Physioljournal_title
Plant physiologyauthors
Kang BH,Busse JS,Dickey C,Rancour DM,Bednarek SYdoi
10.1104/pp.126.1.47subject
Has Abstractpub_date
2001-05-01 00:00:00pages
47-68issue
1eissn
0032-0889issn
1532-2548journal_volume
126pub_type
杂志文章相关文献
PLANT PHYSIOLOGY文献大全abstract::We report n-6 monounsaturated primary alcohols (C26:1, C28:1, and C30:1 homologs) in the cuticular waxes of Arabidopsis (Arabidopsis thaliana) inflorescence stem, a class of wax not previously reported in Arabidopsis. The Arabidopsis cer17 mutant was completely deficient in these monounsaturated alcohols, and CER17 wa...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.16.01956
更新日期:2017-02-01 00:00:00
abstract::Allene oxide synthase (AOS; hydroperoxide dehydratase; EC 4.2.1.92) catalyzes the first step in the biosynthesis of jasmonic acid from lipoxygenase-derived hydroperoxides of free fatty acids. Using the AOS cDNA from tomato (Lycopersicon esculentum), in which the role of jasmonic acid in wound-induced defense gene acti...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.122.4.1335
更新日期:2000-04-01 00:00:00
abstract::Guttation, the loss of water and dissolved materials from uninjured plant organs, is a common phenomenon in higher plants. By using endoplasmic reticulum signal peptides fused to the recombinant protein sequences, we have generated transgenic tobacco (Nicotiana tabacum L. cv Wisconsin) plants that secrete three hetero...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.124.3.927
更新日期:2000-11-01 00:00:00
abstract::Stomatal movements depend on an osmoregulation process in which swelling or shrinking of the guard cells opens or closes the stomatal pore. Ions and water fluxes are an essential aspect of guard cell osmoregulation. Thus far, studies of these fluxes have focused on the guard cell plasma membrane. Guard cells, however,...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.105.3.999
更新日期:1994-07-01 00:00:00
abstract::Embryos from rice (Oryza sativa L. var. Bluebonnet) and wheat (Triticum aestivum L.) contain an aminoacyl-tRNA protein transferase which transfers arginine from arginyl-tRNA to the N terminus of a protein acceptor. The activity was measured in vitro in a reaction mixture containing embryo supernatant fraction, buffer,...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.52.1.13
更新日期:1973-07-01 00:00:00
abstract::In two cultivars of bean (Phaseolus vulgaris L., Redkloud and Redkote) the older fruits growing at the base of racemes aborted less frequently than the younger ones above them. When older fruits at the base of racemes were removed, the abortion rate of the younger ones was reduced and their abscisic acid (ABA) concent...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.64.4.620
更新日期:1979-10-01 00:00:00
abstract::Extraction of red beet root plasma membranes with the detergent Triton X-100 at a level of 2.0% (weight/volume) resulted in the depletion of over 90% of total membrane phospholipid and the reduction of glucan synthase activity by 80 to 90%. Reconstitution of the delipidated Triton X-100, 100,000g fraction in the prese...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.82.2.396
更新日期:1986-10-01 00:00:00
abstract::In sweet pea (Lathyrus odoratus L.) the mutant allele l reduced the level of gibberellin A1 (GA1) in expanding leaflets and resulted in smaller, more oval leaflets compared with the wild type. The apical portions of 6-d-old wild-type (L) seedlings also contained less GA1 and produced smaller, more oval leaflets than d...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.102.2.603
更新日期:1993-06-01 00:00:00
abstract::The use of only the upper part of the hypocotyl and the cotyledons in the Amaranthus tricolor bioassay for cytokinins, instead of the whole seedling, was found to reduce the endogenous response and give a higher benzyladenine-dependent response. There is no marked difference in the uptake or metabolism of benzyladenin...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.63.2.269
更新日期:1979-02-01 00:00:00
abstract::CDP-diglyceride:inositol transferase in endoplasmic reticulum fractions from castor bean (Ricinus communis) endosperm was partially characterized. The enzyme had a pH optimum of 8.5 and required Mn(2+) for activity. Maximal activity was at 1.5 millimolar MnCl(2). A K(m) of 0.30 mM was calculated for myo-inositol and 1...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.62.6.978
更新日期:1978-12-01 00:00:00
abstract::The expression of alternative oxidase (Aox) and uncoupling proteins (Ucp) was investigated during ripening in mango (Mangifera indica) and compared with the expression of peroxisomal thiolase, a previously described ripening marker in mango. The multigene family for the Aox in mango was expressed differentially during...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.126.4.1619
更新日期:2001-08-01 00:00:00
abstract::The light dependent energization of the thylakoid membrane was analyzed in isolated intact spinach (Spinacia oleracea L.) chloroplasts incubated with different concentrations of inorganic phosphate (Pi). Two independent methods were used: (a) the accumulation of [(14)C]5,5-dimethyl-2,4-oxazolidinedione and [(14)C] met...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.91.1.221
更新日期:1989-09-01 00:00:00
abstract::The cloning and analysis of two different cDNA clones encoding putative maize (Zea mays L.) chitinases obtained by polymerase chain reaction (PCR) and cDNA library screening is described. The cDNA library was made from poly(A)+ RNA from leaves challenged with mercuric chloride for 2 d. The two clones, pCh2 and pCh11, ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.105.4.1097
更新日期:1994-08-01 00:00:00
abstract::Indole-3-acetyl-myo-inositol galactoside labeled with 3H in the indole and 14C in the galactose moieties was applied to kernels of 5 day old germinating seedlings of Zea mays. Indole-3-acetyl-myo-inositol galactoside was not transported into either the shoot or root tissue as the intact molecule but was instead hydro...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.80.4.961
更新日期:1986-01-01 00:00:00
abstract::Seed production generally requires the mating of opposite sex gametes. Apomixis, an asexual mode of reproduction, avoids both meiotic reduction and egg fertilization. The essential feature of apomixis is that an embryo is formed autonomously by parthenogenesis from an unreduced egg of an embryo sac generated through a...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.105.062059
更新日期:2005-08-01 00:00:00
abstract::(125)I-Labeled ethylene biosynthesis-inducing xylanase (EIX) was used to study the movement of this protein in tobacco (Nicotiana tabacum) tissues. A biologically active (125)I-labeled EIX was obtained using chloramine-T as the oxidizing agent. Labeled EIX was detected in the far most edges of the leaf 5 min after it ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.100.4.2059
更新日期:1992-12-01 00:00:00
abstract::Pectin methylesterase (PME), polygalacturonase (PG), xylanase, cellulase, and proteinase activity were determined and related to respiration, ethylene evolution, and changes in skin color of papaya (Carica papaya L.) fruit from harvest through to the start of fruit breakdown. PME gradually increased from the start of ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.72.2.382
更新日期:1983-06-01 00:00:00
abstract::Experiments with ethylene-insensitive tomato (Lycopersicon esculentum) and petunia (Petunia x hybrida) plants were conducted to determine if normal or adventitious root formation is affected by ethylene insensitivity. Ethylene-insensitive Never ripe (NR) tomato plants produced more below-ground root mass but fewer abo...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.121.1.53
更新日期:1999-09-01 00:00:00
abstract::The effect of initial oxygen concentration on the rate and extent of oxidation of the respiratory chain carriers of anaerobic mitochondria from mung bean (Phaseolus aureus) seedlings was examined. The substrate was succinate, with malonate added to give malonate to succinate ratios of 6 to 12, thereby minimizing the f...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.50.1.95
更新日期:1972-07-01 00:00:00
abstract::Wood is an important raw material and environmentally cost-effective renewable source of energy. However, the molecular biology of wood formation (i.e. secondary growth) is surprisingly understudied. A novel experimental system was employed to study the molecular regulation of secondary xylem formation in Arabidopsis....
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.104.038844
更新日期:2004-06-01 00:00:00
abstract::The effect of ozone was studied on the peroxidase activity from various compartments of Sedum album leaves (epidermis, intercellular fluid, residual cell material, and total cell material). The greatest increase following a 2-hour ozone exposure (0.4 microliters O(3) per liter) was observed in extracellular peroxidase...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.74.4.846
更新日期:1984-04-01 00:00:00
abstract::Gametophore induction in moss by Agrobacterium tumefaciens was inhibited by addition of lipopolysaccharide (LPS) from A. tumefaciens. The LPS did not affect bacterial viability or appear to bind to bacterial cells. LPS from nonbinding Agrobacterium radiobacter was not effective in reducing gametophore formation. A. tu...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.60.5.765
更新日期:1977-11-01 00:00:00
abstract::This study was performed to test if alternative carbon sources besides recently photosynthetically fixed CO2 are used for isoprene formation in the leaves of young poplar (Populus x canescens) trees. In a 13CO2 atmosphere under steady state conditions, only about 75% of isoprene became 13C labeled within minutes. A co...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.103.037374
更新日期:2004-05-01 00:00:00
abstract::The light dependence of D1 phosphorylation is unique to higher plants, being constitutive in cyanobacteria and algae. In a photoautotrophic higher plant, Spirodela oligorrhiza, grown in greenhouse conditions under natural diurnal cycles of solar irradiation, the ratio of phosphorylated versus total D1 protein (D1-P in...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.013441
更新日期:2002-12-01 00:00:00
abstract::Malformin and ethylene stimulate abscission of the primary leaves of Phaseolus aureus Roxb. in the dark, and abscission stimulation by both compounds is inhibited by indeleacetic acid and CO(2). Ethylene production by malformin-treated buds is stimulated within 4 hours. and up to 8 days, after treatment. Malformin-ind...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.43.1.76
更新日期:1968-01-01 00:00:00
abstract::Ethylene supplied with indoleacetic acid at 0.1 and 1 mum inhibited elongation and enhanced swelling in epicotyls of decapitated and derooted pea seedlings (Pisum sativum L., var. Alaska). These growth responses were correlated with the development of cell walls rich in weak acid-extractable materials and pectic uroni...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.55.3.450
更新日期:1975-03-01 00:00:00
abstract::The oxygen requirements during the three phases of photoinduced germination of Onoclea sensibilis L. spores were analyzed by temporarily applying nitrogen atmosphere. The dark preinduction phase, during which the spores imbibe water and establish sensitivity to irradiation, involves an oxidative process which can be r...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.55.2.150
更新日期:1975-02-01 00:00:00
abstract::Promoters of most seed proteins in legumes contain one or more 5'-CATGCAT-3' elements. To test if these elements have a function in the expression of these genes, the 2.3 kilobase pairs Gy2 glycinin promoter was ligated to a beta-glucuronidase reporter sequence and transformed into tobacco. Elimination of a 5'-CATGCAT...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.98.1.387
更新日期:1992-01-01 00:00:00
abstract::The multifunctional movement protein (MP) of Tomato mosaic tobamovirus (ToMV) is involved in viral cell-to-cell movement, symptom development, and resistance gene recognition. However, it remains to be elucidated how ToMV MP plays such diverse roles in plants. Here, we show that ToMV MP interacts with the Rubisco smal...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.112.209213
更新日期:2013-01-01 00:00:00
abstract::Plant shoot organs arise from initial cells that are recruited from meristematic tissues. Previous studies have shown that members of the WUSCHEL-related HOMEOBOX (WOX) gene family function to organize various initial cell populations during plant development. The function of the WOX4 gene is previously undescribed in...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.109.149641
更新日期:2010-03-01 00:00:00