Abstract:
:Extraction of red beet root plasma membranes with the detergent Triton X-100 at a level of 2.0% (weight/volume) resulted in the depletion of over 90% of total membrane phospholipid and the reduction of glucan synthase activity by 80 to 90%. Reconstitution of the delipidated Triton X-100, 100,000g fraction in the presence of phospholipids restored glucan synthase activity. The most effective phospholipid was phosphatidyl-ethanolamine, which restored 110 to 144% of the original activity at 0.5% (weight/volume). Glucan synthase in the phospholipid-reactivated Triton X-100-treated fraction was enriched 9-fold in specific activity relative to microsomal membranes but was unstable in digitonin. These results support the hypothesis that glucan synthase activity is regulated by its phospholipid environment.
journal_name
Plant Physioljournal_title
Plant physiologyauthors
Wasserman BP,McCarthy KJdoi
10.1104/pp.82.2.396subject
Has Abstractpub_date
1986-10-01 00:00:00pages
396-400issue
2eissn
0032-0889issn
1532-2548journal_volume
82pub_type
杂志文章相关文献
PLANT PHYSIOLOGY文献大全abstract::Microcomplement fixation was employed to compare the immunological differences that occur between purified inhibitor I from potato tubers, the four purified protomers that comprise it, and inhibitor I from tuber and leaf extracts. Total inhibitors of chymotrypsin and trypsin in leaves of seven genera of the Solanaceae...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.50.5.627
更新日期:1972-11-01 00:00:00
abstract::All mutants at 3 loci in Arabidopsis thaliana (L.) Heynh., a higher plant, that are associated with the synthesis or coupling of the thiazole moiety of thiamine are susceptible to reversible glucose inhibition. In contrast, several different alleles involved in the synthesis of the pyrimidine moiety of the vitamin are...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.44.2.225
更新日期:1969-02-01 00:00:00
abstract::An alpha-L-fucosidase (EC 3.2.1.51) able to release the t-fucosyl residue from the side chain of xyloglucan oligosaccharides has been detected in the leaves of Arabidopsis plants. Moreover, an alpha-L-fucosidase with similar substrate specificity was purified from cabbage (Brassica oleracea) leaves to render a single ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:
更新日期:2002-01-01 00:00:00
abstract::The cell walls of wheat (Triticum aestivum) starchy endosperm are dominated by arabinoxylan (AX), accounting for 65% to 70% of the polysaccharide content. Genes within two glycosyl transferase (GT) families, GT43 (IRREGULAR XYLEM9 [IRX9] and IRX14) and GT47 (IRX10), have previously been shown to be involved in the syn...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.113.222653
更新日期:2013-09-01 00:00:00
abstract::XBAT32, a member of the RING domain-containing ankyrin repeat subfamily of E3 ligases, was previously identified as a positive regulator of lateral root development. Arabidopsis (Arabidopsis thaliana) plants harboring a mutation in XBAT32 produce fewer lateral roots that wild-type plants. We found that xbat32 mutants ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.110.156976
更新日期:2010-08-01 00:00:00
abstract::Plant species differ in response to high available manganese (Mn), but the mechanisms of sensitivity and tolerance are poorly understood. In solution culture, greater than or equal to 30 µm Mn decreased the growth of soybean (Glycine max), but white lupin (Lupinus albus), narrow-leafed lupin (Lupin angustifolius), and...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.15.00726
更新日期:2015-11-01 00:00:00
abstract::Using tissue blotting and immunocytolocalization, we have investigated the appearance and accumulation of polygalacturonase (PG) during tomato (Lycopersicon esculentum Mill.) fruit ripening. Results show that PG first appears in the collumella region followed by sequential appearance in the exopericarp and endopericar...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.90.1.17
更新日期:1989-05-01 00:00:00
abstract::Pattern-recognition receptors (PRRs), which consist of receptor kinases (RKs) and receptor-like proteins, sense microbe- and host-derived molecular patterns associated with pathogen infection to trigger immune responses in plants. Several kinases of the 46-member Arabidopsis (Arabidopsis thaliana) receptor-like cytopl...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.18.00486
更新日期:2018-08-01 00:00:00
abstract::The effect of NO2- assimilation on O2 exchange and CO2 fixation of the cyanobacterium, Synechococcus UTEX 625, was studied mass spectrometrically. Upon addition of 1 mM inorganic carbon to the medium, inorganic carbon pools developed and accelerated O2 photoreduction 5-fold when CO2 fixation was inhibited. During stea...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.109.4.1295
更新日期:1995-12-01 00:00:00
abstract::Light induces beta-carotene synthesis in streptomycin-bleached Euglena gracilis Z. Light-adapted, chemostat-grown cells have up to 10-fold as much beta-carotene and 25% more protein than similarly grown, dark-adapted cells. Carotenogenesis does not occur under anaerobic conditions or in the presence of diphenylamine, ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.46.5.685
更新日期:1970-11-01 00:00:00
abstract::Here, we monitor the effects of ectopic overexpression of genes for pea asparagine synthetase (AS1) in transgenic tobacco (Nicotiana tabacum). The AS genes of pea and tobacco are normally expressed only during the dark phase of the diurnal growth cycle and specifically in phloem cells. A hybrid gene was constructed in...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.103.4.1285
更新日期:1993-12-01 00:00:00
abstract::Phosphatidylinositol 3-kinase has been reported to be important for normal plant growth. To characterize the role of the enzyme further, we attempted to isolate Arabidopsis (Arabidopsis thaliana) plants that do not express the gene, but we could not recover homozygous mutant plants. The progeny of VPS34/vps34 heterozy...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.108.121590
更新日期:2008-08-01 00:00:00
abstract::Small GTP-binding proteins regulate diverse processes in eukaryotic cells such as signal transduction, cell proliferation, cytoskeletal organization, and intracellular membrane trafficking. These proteins function as molecular switches that cycle between "active" and "inactive" states, and this cycle is linked to the ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.013052
更新日期:2003-03-01 00:00:00
abstract::We have identified two novel periplasmic/cell wall polypeptides that specifically accumulate during sulfur limitation of Chlamydomonas reinhardtii. These polypeptides, present at high levels in the extracellular polypeptide fraction from a sulfur-deprived, cell wall-minus C. reinhardtii strain, have apparent molecular...
journal_title:Plant physiology
pub_type: 杂志文章
doi:
更新日期:2001-10-01 00:00:00
abstract::Calcium flux in sunflower (Helianthus annuus L. cv Russian mammoth) hypocotyl was measured with a Ca(2+) electrode as the increase or decrease in Ca(2+) in an aqueous solution (10 micromolar CaCl(2)) in contact with either the basal or apical end of 20 millimeter segments. Ca(2+) efflux was significantly higher at the...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.81.2.408
更新日期:1986-06-01 00:00:00
abstract::Very low ethylene production rates were measured in nonpollinated Cyclamen persicum Mill flowers, and no change in production was observed during the whole life span of the flower until death. Normal senescence was accompanied by a gradual discoloration and loss of turgor followed by wilting. Pollination induced a dra...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.75.4.1090
更新日期:1984-08-01 00:00:00
abstract::Plant defense mechanisms against necrotrophic pathogens, such as Botrytis cinerea, are considered to be complex and to differ from those that are effective against biotrophs. In the abscisic acid-deficient sitiens tomato (Solanum lycopersicum) mutant, which is highly resistant to B. cinerea, accumulation of hydrogen p...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.107.099226
更新日期:2007-08-01 00:00:00
abstract::Sperm cells have been isolated from pollen tubes growing in style segments of the dicotlyledon Rhododendron macgregoriae and the monocotyledon Gladiolus gandavensis by the in vivo/in vitro method at various stages of fertilization. Pollen tubes emerged from the cut end of the style into agar medium, and more than 95% ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.87.3.647
更新日期:1988-07-01 00:00:00
abstract::Using intact leaves of Spinacia oleracea (L.), reversible temperature-induced changes in chlorophyll fluorescence emitted at room temperature and at 77K were studied. Interpretation of fluorescence at 77K was largely facilitated by developing a new method to minimize reabsorption artifacts (;diluted leaf-powder'). Lea...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.74.2.402
更新日期:1984-02-01 00:00:00
abstract::The OsCPK4 gene is a member of the complex gene family of calcium-dependent protein kinases in rice (Oryza sativa). Here, we report that OsCPK4 expression is induced by high salinity, drought, and the phytohormone abscisic acid. Moreover, a plasma membrane localization of OsCPK4 was observed by transient expression as...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.113.230268
更新日期:2014-06-01 00:00:00
abstract::The incorporation of [(14)C]phenylalanine and [1,6-(14)C]shikimic acid into tyrosine was investigated in the callus of Portulaca grandiflora, var. JR (L.). By inhibiting phenylalanine with 1-alpha-aminooxy-beta-phenyl-propionic acid and tyrosinase with 1-cysteine-HCl and hydrazine-hydrate, the possible synthesis of ty...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.68.2.272
更新日期:1981-08-01 00:00:00
abstract::Cinnamate-4-hydroxylase (C4H) is the first Cyt P450-dependent monooxygenase of the phenylpropanoid pathway. To study the expression of this gene in Arabidopsis thaliana, a C4H cDNA clone from the Arabidopsis expressed sequence tag database was identified and used to isolate its corresponding genomic clone. The entire ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.113.3.729
更新日期:1997-03-01 00:00:00
abstract::Plants respond to low carbon supply by massive reprogramming of the transcriptome and metabolome. We show here that the carbon starvation-induced NAC (for NO APICAL MERISTEM/ARABIDOPSIS TRANSCRIPTION ACTIVATION FACTOR/CUP-SHAPED COTYLEDON) transcription factor Arabidopsis (Arabidopsis thaliana) Transcription Activatio...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.15.00917
更新日期:2015-09-01 00:00:00
abstract::Nicotianamine (NA) occurs in all plants and chelates metal cations, including FeII, but reportedly not FeIII. However, a comparison of the FeII and ZnII affinity constants of NA and various FeIII-chelating aminocarboxylates suggested that NA should chelate FeIII. High-voltage electrophoresis of the FeNA complex formed...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.119.3.1107
更新日期:1999-03-01 00:00:00
abstract::N(6)-(Delta(2)-isopentenyl)Adenine and N(6)-(Delta(2)-isopentenyl)adenosine were hydroxylated, respectively, to 6-(4-hydroxy-3-methyl-trans-2-butenylamino)purine and 6-(4-hydroxy-3-methyl-trans-2-butenylamino) -9-beta-ribofuranosylpurine in the presence of NADPH and the microsomal fraction from cauliflowers (Brassica ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.75.2.442
更新日期:1984-06-01 00:00:00
abstract::The relationship between gravitropism and surface electrical potentials was studied using etiolated epicotyls of adzuki bean (Phaseolus angularis). Early downward curvature (or transient positive gravitropic response) was observed about 1 min after gravistimulation. The downward curvature was closely related to the sp...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.105.3.875
更新日期:1994-07-01 00:00:00
abstract::Promoters of most seed proteins in legumes contain one or more 5'-CATGCAT-3' elements. To test if these elements have a function in the expression of these genes, the 2.3 kilobase pairs Gy2 glycinin promoter was ligated to a beta-glucuronidase reporter sequence and transformed into tobacco. Elimination of a 5'-CATGCAT...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.98.1.387
更新日期:1992-01-01 00:00:00
abstract::The evolution of C4 photosynthesis in many taxa involves the establishment of a two-celled photorespiratory CO2 pump, termed C2 photosynthesis. How C3 species evolved C2 metabolism is critical to understanding the initial phases of C4 plant evolution. To evaluate early events in C4 evolution, we compared leaf anatomy,...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.113.221119
更新日期:2013-11-01 00:00:00
abstract::With an experimental system developed for aquatic plants using the mass spectrometry technique and infrared gas analysis of CO(2), we studied the responses to various O(2) concentrations of gas exchanges with the red macroalga Chondrus crispus S. The results were as follows. (a) Irrespective of the CO(2) concentration...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.78.3.545
更新日期:1985-07-01 00:00:00
abstract::In culture, the phytopathogenic fungus Phytophthora cryptogea secretes a protein which elicits hypersensitive-like necroses and protects tobacco plants against invasion by the pathogen Phytophthora parasitica var. nicotianae. This protein, named cryptogein, has been purified and its amino acid sequence determined. In ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.95.2.486
更新日期:1991-02-01 00:00:00