The Arabidopsis phosphatidylinositol 3-kinase is important for pollen development.

Abstract:

:Phosphatidylinositol 3-kinase has been reported to be important for normal plant growth. To characterize the role of the enzyme further, we attempted to isolate Arabidopsis (Arabidopsis thaliana) plants that do not express the gene, but we could not recover homozygous mutant plants. The progeny of VPS34/vps34 heterozygous plants, harboring a T-DNA insertion, showed a segregation ratio of 1:1:0 for wild-type, heterozygous, and homozygous mutant plants, indicating a gametophytic defect. Genetic transmission analysis showed that the abnormal segregation ratio was due to failure to transmit the mutant allele through the male gametophyte. Microscopic observation revealed that 2-fold higher proportions of pollen grains in heterozygous plants than wild-type plants were dead or showed reduced numbers of nuclei. Many mature pollen grains from the heterozygous plants contained large vacuoles even until the mature pollen stage, whereas pollen from wild-type plants contained many small vacuoles beginning from the vacuolated pollen stage, which indicated that vacuoles in many of the heterozygous mutant pollen did not undergo normal fission after the first mitotic division. Taken together, our results suggest that phosphatidylinositol 3-kinase is essential for vacuole reorganization and nuclear division during pollen development.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Lee Y,Kim ES,Choi Y,Hwang I,Staiger CJ,Chung YY,Lee Y

doi

10.1104/pp.108.121590

subject

Has Abstract

pub_date

2008-08-01 00:00:00

pages

1886-97

issue

4

eissn

0032-0889

issn

1532-2548

pii

pp.108.121590

journal_volume

147

pub_type

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