Roles of Receptor-Like Cytoplasmic Kinase VII Members in Pattern-Triggered Immune Signaling.

Abstract:

:Pattern-recognition receptors (PRRs), which consist of receptor kinases (RKs) and receptor-like proteins, sense microbe- and host-derived molecular patterns associated with pathogen infection to trigger immune responses in plants. Several kinases of the 46-member Arabidopsis (Arabidopsis thaliana) receptor-like cytoplasmic kinase (RLCK) subfamily VII play important roles in pattern-triggered immunity, but it is unclear whether different RLCK VII members act specifically or redundantly in immune signaling. Here, we constructed nine higher order mutants of this subfamily (named rlck vii-1 to rlck vii-9) and systematically characterized their immune phenotypes. The mutants rlck vii-5, -7, and -8 had compromised reactive oxygen species production in response to all patterns tested, indicating that the corresponding members are broadly required for the signaling of multiple PRRs. However, rlck vii-4 was defective specifically in chitin-induced reactive oxygen species production, suggesting that RCLK VII-4 members mediate the signaling of specific PRRs. Furthermore, RLCK VII-4 members were required for the chitin-triggered activation of MAPK, demonstrating that these kinases link a PRR to MAPK activation. Moreover, we found that RLCK VII-6 and -8 also were required for RK-mediated root growth. Together, these results show that numerous RLCK VII members are involved in pattern-triggered immune signaling and uncover both common and specific roles of these kinases in plant development and immunity mediated by various RKs.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Rao S,Zhou Z,Miao P,Bi G,Hu M,Wu Y,Feng F,Zhang X,Zhou JM

doi

10.1104/pp.18.00486

subject

Has Abstract

pub_date

2018-08-01 00:00:00

pages

1679-1690

issue

4

eissn

0032-0889

issn

1532-2548

pii

pp.18.00486

journal_volume

177

pub_type

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