Characteristics of ethylene biosynthesis-inducing xylanase movement in tobacco leaves.

Abstract:

:(125)I-Labeled ethylene biosynthesis-inducing xylanase (EIX) was used to study the movement of this protein in tobacco (Nicotiana tabacum) tissues. A biologically active (125)I-labeled EIX was obtained using chloramine-T as the oxidizing agent. Labeled EIX was detected in the far most edges of the leaf 5 min after it was applied to the petiole of a detached leaf. EIX was distributed uniformly throughout the leaf, including the mesophyll area within 5 to 15 min, after which there was only little change in the distribution of radioactivity in the leaf. (125)I-Labeled EIX was extracted from treated leaves, and EIX translocation in the leaf was blocked by preincubation of labeled EIX with anti-EIX antibodies, indicating that the intact peptide moves in the leaf. Injection of anti-EIX antibodies into the intercellular spaces of the leaf mesophyll prevented induction of necrosis by EIX, suggesting the mesophyll as the site of EIX action. EIX was translocated both to upper and lower parts of the plant when applied to a whole plant through the petiole of a cut leaf. Radioactivity was found in all leaves and in the stem, although some leaves accumulated much more EIX than others; EIX was not found in the roots. There was no difference between the accumulation pattern of EIX in fresh and ethylene-treated leaves or between sensitive (Xanthi) and insensitive (Hicks) tobacco cultivars. These data support the hypothesis that intact EIX protein is translocated to the leaf mesophyll, where it directly elicits plant defense responses.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Sharon A,Bailey BA,McMurtry JP,Taylor R,Anderson JD

doi

10.1104/pp.100.4.2059

subject

Has Abstract

pub_date

1992-12-01 00:00:00

pages

2059-65

issue

4

eissn

0032-0889

issn

1532-2548

journal_volume

100

pub_type

杂志文章
  • SAGE analysis of transcriptome responses in Arabidopsis roots exposed to 2,4,6-trinitrotoluene.

    abstract::Serial analysis of gene expression was used to profile transcript levels in Arabidopsis roots and assess their responses to 2,4,6-trinitrotoluene (TNT) exposure. SAGE libraries representing control and TNT-exposed seedling root transcripts were constructed, and each was sequenced to a depth of roughly 32,000 tags. Mor...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.103.028019

    authors: Ekman DR,Lorenz WW,Przybyla AE,Wolfe NL,Dean JF

    更新日期:2003-11-01 00:00:00

  • Hydroperoxide Lyase and Other Hydroperoxide-Metabolizing Activity in Tissues of Soybean, Glycine max.

    abstract::Hydroperoxide lyase (HPLS) activity in soybean (Glycine max) seed/seedlings, leaves, and chloroplasts of leaves required detergent solubilization for maximum in vitro activity. On a per milligram of protein basis, more HPLS activity was found in leaves, especially chloroplasts, than in seeds or seedlings. The total yi...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.97.3.1059

    authors: Gardner HW,Weisleder D,Plattner RD

    更新日期:1991-11-01 00:00:00

  • Constitutive activation of transcription factor OsbZIP46 improves drought tolerance in rice.

    abstract::OsbZIP46 is one member of the third subfamily of bZIP transcription factors in rice (Oryza sativa). It has high sequence similarity to ABA-responsive element binding factor (ABF/AREB) transcription factors ABI5 and OsbZIP23, two transcriptional activators positively regulating stress tolerance in Arabidopsis (Arabidop...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.111.190389

    authors: Tang N,Zhang H,Li X,Xiao J,Xiong L

    更新日期:2012-04-01 00:00:00

  • A circuit analog model for studying quantitative water relations of plant tissues.

    abstract::Using arrays of resistors and capacitors, a lumped circuit analog of plant tissue is developed. The circuit elements of the analog are identified in terms of physiological variables (hydraulic conductivities, water capacities, and cell dimensions) which can be measured in the laboratory. With the aid of a circuit simu...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.64.5.712

    authors: Molz FJ,Kerns DV,Peterson CM,Dane JH

    更新日期:1979-11-01 00:00:00

  • Mercury-induced Ethylene Formation and Abscission in Citrus and Coleus Explants.

    abstract::Mercury vapor induces ethylene formation and abscission in Citrus and Coleus explants. Both responses are markedly greater in the absence of CO(2). The stimulation of these metabolically complex processes indicates that the action of mercury vapor is not consistent with the more popular conception of mercury toxicity....

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.57.4.628

    authors: Goren R,Siegel SM

    更新日期:1976-04-01 00:00:00

  • CER16 Inhibits Post-Transcriptional Gene Silencing of CER3 to Regulate Alkane Biosynthesis.

    abstract::The aerial surfaces of land plants have a protective layer of cuticular wax. Alkanes are common components of these waxes, and their abundance is affected by a range of stresses. The CER16 protein has been implicated in alkane biosynthesis in the cuticular wax of Arabidopsis (Arabidopsis thaliana). Here, we identified...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.19.01002

    authors: Yang X,Feng T,Li S,Zhao H,Zhao S,Ma C,Jenks MA,Lü S

    更新日期:2020-03-01 00:00:00

  • Overexpression of ca1pase Decreases Rubisco Abundance and Grain Yield in Wheat.

    abstract::Rubisco catalyzes the fixation of CO2 into organic compounds that are used for plant growth and the production of agricultural products, and specific sugar-phosphate derivatives bind tightly to the active sites of Rubisco, locking the enzyme in a catalytically inactive conformation. 2-carboxy-d-arabinitol-1-phosphate ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.19.00693

    authors: Lobo AKM,Orr DJ,Gutierrez MO,Andralojc PJ,Sparks C,Parry MAJ,Carmo-Silva E

    更新日期:2019-10-01 00:00:00

  • Water Use Efficiency of Field-grown Maize during Moisture Stress.

    abstract::Theoretical analysis of the CO(2) assimilation and water loss by single leaves suggests that the water use efficiency of C(4) species decreases as stomatal resistance increases. To confirm this hypothesis for a complete maize crop, results from computer simulations and a field experiment were compiled for varying stom...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.56.2.245

    authors: Sinclair TR,Bingham GE,Lemon ER,Allen LH

    更新日期:1975-08-01 00:00:00

  • Abscisic acid and stress signals induce Viviparous1 expression in seed and vegetative tissues of maize.

    abstract::Viviparous1 (Vp1) encodes a B3 domain-containing transcription factor that is a key regulator of seed maturation in maize (Zea mays). However, the mechanisms of Vp1 regulation are not well understood. To examine physiological factors that may regulate Vp1 expression, transcript levels were monitored in maturing embryo...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.106.091454

    authors: Cao X,Costa LM,Biderre-Petit C,Kbhaya B,Dey N,Perez P,McCarty DR,Gutierrez-Marcos JF,Becraft PW

    更新日期:2007-02-01 00:00:00

  • Phytoluminographic detection of dynamic variations in leaf gaseous conductivity.

    abstract::Gas exchange and plant luminescence (delayed light emission) of a single red kidney bean leaf undergoing synchronous oscillations in gas exchange were recorded and analyzed. Introduction of 1.1 microliter per liter SO(2) during these oscillations produced increases in plant luminescence that, when averaged over a port...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.78.4.904

    authors: Ellenson JL

    更新日期:1985-08-01 00:00:00

  • Release of Photosynthetic Protein Catabolites by Blebbing from Thylakoids.

    abstract::Thylakoid proteins and their catabolites have been detected in lipid-protein particles isolated from the stroma of intact chloroplasts obtained from primary leaves of 2-week-old bean seedlings (Phaseolus vulgaris L. cv Kinghorn). The lipid-protein particles bear morphological resemblance to plastoglobuli seen in the c...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.106.4.1547

    authors: Ghosh S,Hudak KA,Dumbroff EB,Thompson JE

    更新日期:1994-12-01 00:00:00

  • Glutamine metabolism in corn kernels cultured in vitro.

    abstract::The fate of glutamine, the major source of nitrogen supplied to the developing maize endosperm, has been examined in endosperm tissues of corn caryopsis grown under sterile conditions. In the culture system, [U-(14)C]glutamine was included in the medium or was injected directly into the endosperm. Samples were harvest...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.77.3.520

    authors: Misra S,Oaks A

    更新日期:1985-03-01 00:00:00

  • The out of phase 1 mutant defines a role for PHYB in circadian phase control in Arabidopsis.

    abstract::Arabidopsis displays circadian rhythms in stomatal aperture, stomatal conductance, and CO(2) assimilation, each of which peaks around the middle of the day. The rhythmic opening and closing of stomata confers a rhythm in sensitivity and resistance, respectively, to the toxic gas sulfur dioxide. Using this physiologica...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.003418

    authors: Salomé PA,Michael TP,Kearns EV,Fett-Neto AG,Sharrock RA,McClung CR

    更新日期:2002-08-01 00:00:00

  • Regulation of Pyrimidine Biosynthesis in Intact Cells of Cucurbita pepo.

    abstract::The occurrence of the complete orotic acid pathway for the biosynthesis de novo of pyrimidine nucleotides was demonstrated in the intact cells of roots excised from summer squash (Cucurbita pepo L. cv. Early Prolific Straightneck). Evidence that the biosynthesis of pyrimidine nucleotides proceeds via the orotate pathw...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.64.4.562

    authors: Lovatt CJ,Albert LS

    更新日期:1979-10-01 00:00:00

  • Short-Term Regulation of Crassulacean Acid Metabolism Activity in a Tropical Hemiepiphyte, Clusia uvitana.

    abstract::Diel courses of net CO2 exchange of leaves were studied in Clusia uvitana (Clusiaceae), a tropical Crassulacean acid metabolism (CAM) hemiepiphyte, growing in the crown of a 47-m tall kapok tree on Barro Colorado Island, Panama. Measurements on days without precipitation showed that net uptake of atmospheric CO2 occur...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.102.3.835

    authors: Zotz G,Winter K

    更新日期:1993-07-01 00:00:00

  • Metabolism of Monoterpenes : Early Steps in the Metabolism of d-Neomenthyl-beta-d-Glucoside in Peppermint (Mentha piperita) Rhizomes.

    abstract::Previous studies have shown that the monoterpene ketone l-[G-(3)H] menthone is reduced to the epimeric alcohols l-menthol and d-neomenthol in leaves of flowering peppermint (Mentha piperita L.), and that a portion of the menthol is converted to menthyl acetate while the bulk of the neomenthol is transformed to neoment...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.76.3.647

    authors: Croteau R,Sood VK,Renstrøm B,Bhushan R

    更新日期:1984-11-01 00:00:00

  • High Energy Charge as a Requirement for Axis Elongation in Response to Gibberellic Acid and Kinetin during Stratification of Acer saccharum Seeds.

    abstract::The growth potential of embryonic axes of Acer saccharum Marsh. increased during moist storage at 5 C but not at 20 C. During the period of increasing growth potential, the oxygen consumption of the axes remained constant. It was possible to distinguish three phases of the stratification-germination process at 5 C wit...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.53.1.91

    authors: Simmonds JA,Dumbroff EB

    更新日期:1974-01-01 00:00:00

  • Influence of s-Triazines on Some Enzymes of Carbohydrates and Nitrogen Metabolism in Leaves of Pea (Pisum sativum L.) and Sweet Corn (Zea mays L.).

    abstract::Foliar applications of 2 milligrams per liter of 2-chloro-4,6-bis (ethylamino)-s-triazine, 2-methylmercapto-4-ethylamino-6-isobutylamino-s-triazine, and 2-methoxy-4-isopropylamino-6-butylamino-s-triazine caused increases in the activities of starch phosphorylase, pyruvate kinase, cytochrome oxidase, and glutamate dehy...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.48.5.517

    authors: Wu MT,Singh B,Salunkhe DK

    更新日期:1971-11-01 00:00:00

  • The onset of gravisensitivity in the embryonic root of flax.

    abstract::Vertical orientation of emerging roots typically is the first response of plants to gravity. Although root gravitropism has been studied extensively, no conclusive data on the onset of gravisensing exist. We determined the inception of gravisensitivity in flax (Linum usitatissimum) roots by clinorotating germinating s...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.105.073296

    authors: Ma Z,Hasenstein KH

    更新日期:2006-01-01 00:00:00

  • Characterization of Blue-Green Fluorescence in the Mesophyll of Sugar Beet (Beta vulgaris L.) Leaves Affected by Iron Deficiency.

    abstract::The mesophyll of sugar beet (Beta vulgaris L.) leaves emits red (chlorophyll a) fluorescence and blue-green fluorescence when excited with ultraviolet light. The intensity of blue-green fluorescence was increased in mesophylls affected by iron deficiency. This increase was large and progressive. It was concomitant wit...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.106.1.127

    authors: Morales F,Cerovic ZG,Moya I

    更新日期:1994-09-01 00:00:00

  • Studies on the 70S Ribosomal Content of a Plastid Mutant in Gossypium hirsutum.

    abstract::Analysis of a mutation in cotton (Gossypium hirsutum), which is maternally inherited, revealed that the completely white sectors of leaves were deficient in the 70S class ribosomes, whereas the yellow sectors exhibited the same level of the latter as in the green leaves. ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.51.6.1138

    authors: Katterman FR,Endrizzi JE

    更新日期:1973-06-01 00:00:00

  • The lys5 mutations of barley reveal the nature and importance of plastidial ADP-Glc transporters for starch synthesis in cereal endosperm.

    abstract::Much of the ADP-Glc required for starch synthesis in the plastids of cereal endosperm is synthesized in the cytosol and transported across the plastid envelope. To provide information on the nature and role of the plastidial ADP-Glc transporter in barley (Hordeum vulgare), we screened a collection of low-starch mutant...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.104.045203

    authors: Patron NJ,Greber B,Fahy BF,Laurie DA,Parker ML,Denyer K

    更新日期:2004-08-01 00:00:00

  • Potassium Transport in Corn Roots : III. Perturbation by Exogenous NADH and Ferricyanide.

    abstract::It has recently been reported that plasmalemma electron transport may be involved in the generation of H(+) gradients and the uptake of ions into root tissue. We report here on the influence of extracellular NADH and ferricyanide on K(+) ((86)Rb(+)) influx, K(+) ((86)Rb(+)) efflux, net apparent H(+) efflux, and O(2) c...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.77.2.429

    authors: Kochian LV,Lucas WJ

    更新日期:1985-02-01 00:00:00

  • An intermediate in the synthesis of glucobrassicins from 3-indoleacetaldoxime by woad leaves.

    abstract::Leaves of woad (Isatis tinctoria L.) were found to incorporate efficiently tritiated indoleacetaldoxime and (35)S from (35)S-l-cystine into glucobrassicin and sulfoglucobrassicin. Time course of incorporation of (35)S from (35)S-cystine into the glucosinolates indicated that glucobrassicin was formed first and then su...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.50.1.43

    authors: Mahadevan S,Stowe BB

    更新日期:1972-07-01 00:00:00

  • Influence of Free Fatty Acids, Lysophosphatidylcholine, Platelet-Activating Factor, Acylcarnitine, and Echinocandin B on 1,3-beta-d-Glucan Synthase and Callose Synthesis.

    abstract::The activity of 1,3-beta-d-glucan synthase assayed in the presence of digitonin in a microsomal preparation from suspension-cultured cells of Glycine max can be fully inhibited by unsaturated fatty acids, trienoic acids being most effective. Lysophosphatidylcholine, platelet-activating factor, acylcarnitine, and Echin...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.80.1.7

    authors: Kauss H,Jeblick W

    更新日期:1986-01-01 00:00:00

  • Functional and expressional analyses of PmDAM genes associated with endodormancy in Japanese apricot.

    abstract::Bud endodormancy in woody plants plays an important role in their perennial growth cycles. We previously identified a MADS box gene, DORMANCY-ASSOCIATED MADS box6 (PmDAM6), expressed in the endodormant lateral buds of Japanese apricot (Prunus mume), as a candidate for the dormancy-controlling gene. In this study, we d...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.111.181982

    authors: Sasaki R,Yamane H,Ooka T,Jotatsu H,Kitamura Y,Akagi T,Tao R

    更新日期:2011-09-01 00:00:00

  • Multiple Integrated Root Phenotypes Are Associated with Improved Drought Tolerance.

    abstract::To test the hypothesis that multiple integrated root phenotypes would co-optimize drought tolerance, we phenotyped the root anatomy and architecture of 400 mature maize (Zea mays) genotypes under well-watered and water-stressed conditions in the field. We found substantial variation in all 23 root phenes measured. A p...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.20.00211

    authors: Klein SP,Schneider HM,Perkins AC,Brown KM,Lynch JP

    更新日期:2020-07-01 00:00:00

  • De novo synthesis of 3'-nucleotidase in germinating wheat embryo.

    abstract::The enzyme 3'-AMP nucleotidase was purified 2,500- to 5,000-fold from extracts of an acetone powder of wheat (Triticum aestivum) embryonic axes germinated for 40 hours. Sodium dodecyl sulfate acrylamide gel electrophoresis and chromatography on Biogel-P100 indicate that the enzyme is monomeric with a molecular weight ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.71.2.295

    authors: Zhu ZP,Marsh L,Marcus A

    更新日期:1983-02-01 00:00:00

  • Compromised virus-induced gene silencing in RDR6-deficient plants.

    abstract::RNA silencing in plants serves as a potent antiviral defense mechanism through the action of small interfering RNAs (siRNAs), which direct RNA degradation. siRNAs can be derived directly from the viral genome or via the action of host-encoded RNA-dependent RNA polymerases (RDRs). Plant genomes encode multiple RDRs, an...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.108.132688

    authors: Vaistij FE,Jones L

    更新日期:2009-03-01 00:00:00

  • Effect of Inhibition of Glycosylation on the Appearance of a 60 kD Membrane Glycopolypeptide in Endomembrane Fractions of Soybean Root.

    abstract::Endomembrane (endoplasmic reticulum, Golgi apparatus, plasma membrane) proteins of soybean (Glycine max) root cells are highly glycosylated. We investigated whether N-linked oligosaccharide moieties are essential for the correct intracellular transport of plant endomembrane glycoproteins. Excised roots were incubated ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.89.1.299

    authors: Lafayette PR,Travis RL

    更新日期:1989-01-01 00:00:00