Novel Technique for Measuring Tissue Firmness within Tomato (Lycopersicon esculentum Mill.) Fruit.

Abstract:

:Developmental changes of tomato (Lycopersicon esculentum) fruit tissues during maturation were analyzed by a physically defined method (stress-relaxation analysis). The tip of a conical probe connected to a load sensor was positioned on the cut surface of a sliced tomato fruit, and the decay of the imposed stress was monitored. Stress-relaxation data thus obtained were used for the calculation of three stress-relaxation parameters. Different zones within tomato fruit harvested at six different ripening stages were analyzed. One of the stress-relaxation parameters, minimum stress-relaxation time (T(0)), decreased as the fruits matured. The decrease in T(0) was first found in the core of the carpel junction within the endopericarp at the blossom end during the breaker stage. The decrease in T(0) progressed from the blossom end, through the equatorial region and finally throughout the shoulder, as the fruit matured. In mature green fruit, T(0) values within the placenta and the proximal carpel junction were lower than those by other parts of the fruit. For all measurements the maximum stress-relaxation time was not substantially changed during maturation, nor were their changes observed in different regions of the fruit. The observed relaxation rate was therefore correlated with softening. The results indicate that fruit softening may be physically associated with the stress-relaxation parameter, T(0), and the extent of softening is a function of position within the fruit. Decreases in T(0) value appear to be correlated with the reported regional variation in the appearance of polygalacturonase.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Kojima K,Sakurai N,Kuraishi S,Yamamoto R,Nevins DJ

doi

10.1104/pp.96.2.545

subject

Has Abstract

pub_date

1991-06-01 00:00:00

pages

545-50

issue

2

eissn

0032-0889

issn

1532-2548

journal_volume

96

pub_type

杂志文章
  • Loss of starch granule initiation has a deleterious effect on the growth of arabidopsis plants due to an accumulation of ADP-glucose.

    abstract::STARCH SYNTHASE4 (SS4) is required for proper starch granule initiation in Arabidopsis (Arabidopsis thaliana), although SS3 can partially replace its function. Unlike other starch-deficient mutants, ss4 and ss3/ss4 mutants grow poorly even under long-day conditions. They have less chlorophyll and carotenoids than the ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.113.223420

    authors: Ragel P,Streb S,Feil R,Sahrawy M,Annunziata MG,Lunn JE,Zeeman S,Mérida Á

    更新日期:2013-09-01 00:00:00

  • Phloem Transport of Amino Acids in Relation to their Cytosolic Levels in Barley Leaves.

    abstract::A comparison of barley (Hordeum vulgare L.) leaves was made between the cytosolic content of amino acids and sucrose as determined by subcellular fractionation and the corresponding concentration in phloem sap, which was collected continuously for up to 6 days from severed aphid stylets. Because amino acids were found...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.99.3.996

    authors: Winter H,Lohaus G,Heldt HW

    更新日期:1992-07-01 00:00:00

  • Metabolism of Benzyladenine is Impaired in a Mutant of Arabidopsis thaliana Lacking Adenine Phosphoribosyltransferase Activity.

    abstract::Formation of the riboside-5'-monophosphate is a general feature of the metabolism of cytokinins in plants. As part of a study of the biological significance of the nucleotide form of cytokinins, we analyzed a mutant of Arabidopsis thaliana deficient in adenine phosphoribosyltransferase (APRT) activity for its ability ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.95.3.900

    authors: Moffatt B,Pethe C,Laloue M

    更新日期:1991-03-01 00:00:00

  • The kinetics of amiben absorption and metabolism as related to species sensitivity.

    abstract::The rates of absorption and metabolism of 3-amino-2,5-dichlorobenzoic acid (amiben) were investigated in 3-day-old roots of amiben-sensitive velvetleaf (Abutilon theophrasti Medic.) and amiben-tolerant morningglory [Ipomoea hederacea (L.) Jacq.].The initial rates of amiben absorption and binding were identically conce...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.44.6.854

    authors: Stoller EW

    更新日期:1969-06-01 00:00:00

  • The role of pheophorbide a oxygenase expression and activity in the canola green seed problem.

    abstract::Under normal field growth conditions, canola (Brassica napus) seeds produce chloroplasts during early seed development and then catabolize the photosynthetic machinery during seed maturation, producing mature seeds at harvest that are essentially free of chlorophyll (Chl). However, frost exposure early in canola seed ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.106.084483

    authors: Chung DW,Pruzinská A,Hörtensteiner S,Ort DR

    更新日期:2006-09-01 00:00:00

  • A Phytochrome B-Independent Pathway Restricts Growth at High Levels of Jasmonate Defense.

    abstract::The plant hormone jasmonate (JA) promotes resistance to biotic stress by stimulating the degradation of JASMONATE ZIM-DOMAIN (JAZ) proteins, which relieves repression on MYC transcription factors that execute defense programs. JA-triggered depletion of JAZ proteins in Arabidopsis (Arabidopsis thaliana) is also associa...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.19.01335

    authors: Major IT,Guo Q,Zhai J,Kapali G,Kramer DM,Howe GA

    更新日期:2020-06-01 00:00:00

  • Influence of Excision and Aging upon K Influx into Barley Roots: Recovery or Enhancement?

    abstract::The influx of K(+) from (86)Rb-labeled solutions in the concentration range 0.008 to 0.2 mm into roots of intact plants and excised roots of barley plants (Hordeum vulgare [L.]) previously grown in 5 mm CaSO(4) (low K(+) roots) or 0.5 mm CaSO(4) plus 5 mm KCl (high K(+) roots) was measured. A consistent observation of...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.61.4.481

    authors: Glass AD

    更新日期:1978-04-01 00:00:00

  • Tri-Hydroxy-Triacylglycerol Is Efficiently Produced by Position-Specific Castor Acyltransferases.

    abstract::Understanding the biochemistry of triacylglycerol (TAG) assembly is critical in tailoring seed oils to produce high-value products. Hydroxy-fatty acid (HFA) is one such valuable modified fatty acid, which can be produced at low levels in Arabidopsis (Arabidopsis thaliana) seed through transgenic expression of the cast...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.18.01409

    authors: Lunn D,Wallis JG,Browse J

    更新日期:2019-03-01 00:00:00

  • Structural and Functional Insights into a Lysine Deacylase in the Cyanobacterium Synechococcus sp. PCC 7002.

    abstract::Lys deacylases are essential regulators of cell biology in many contexts. Here, we have identified CddA (cyanobacterial deacetylase/depropionylase), a Lys deacylase enzyme expressed in the cyanobacterium Synechococcus sp. PCC 7002 that has both deacetylase and depropionylase activity. Loss of the gene cddA led to slow...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.20.00583

    authors: Liu X,Yang M,Liu Y,Ge F,Zhao J

    更新日期:2020-10-01 00:00:00

  • Phloem mobility of magnesium.

    abstract::Magnesium-28 was applied to specific leaves of bean (Phaseolus vulgaris) and barley (Hordeum vulgare) plants. After 24 hours, as much as 7% of the absorbed Mg was exported from the treated bean leaves and 11% was transported basipetally from the treated zone of the barley leaves. Transport of Mg did not occur past a h...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.46.1.50

    authors: Steucek GL,Koontz HV

    更新日期:1970-07-01 00:00:00

  • Expression of bar in the plastid genome confers herbicide resistance.

    abstract::Phosphinothricin (PPT) is the active component of a family of environmentally safe, nonselective herbicides. Resistance to PPT in transgenic crops has been reported by nuclear expression of a bar transgene encoding phosphinothricin acetyltransferase, a detoxifying enzyme. We report here expression of a bacterial bar g...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.125.4.1585

    authors: Lutz KA,Knapp JE,Maliga P

    更新日期:2001-04-01 00:00:00

  • Ethylene insensitivity conferred by the Green-ripe and Never-ripe 2 ripening mutants of tomato.

    abstract::The ripening of a fleshy fruit represents the summation of an array of biochemical processes that are regulated by interactions between developmental programs and environmental inputs. Analysis of tomato (Solanum lycopersicum) mutants and inhibitor studies indicate that ethylene is necessary for full development of th...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.104.057745

    authors: Barry CS,McQuinn RP,Thompson AJ,Seymour GB,Grierson D,Giovannoni JJ

    更新日期:2005-05-01 00:00:00

  • Response of the Succulent Leaves of Peperomia magnoliaefolia to Dehydration: Water Relations and Solute Movement in Chlorenchyma and Hydrenchyma.

    abstract::Relative water content, solute concentrations, and osmolality were determined in the water storage tissue (hydrenchyma) and the assimilatory tissue (chlorenchyma) of the succulent leaves of Peperomia magnoliaefolia (Jac) (Piperaceae) during slow desiccation. Relative water loss was significantly greater for the hydren...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.83.1.190

    authors: Schmidt JE,Kaiser WM

    更新日期:1987-01-01 00:00:00

  • Transport of sodium into the xylem exudate of tobacco.

    abstract::When tobacco (Nicotiana tabacum L. var. Virginia Gold) plants were pretreated with Na ((22)Na) several days before detopping, from 2.3 to 4.9% of Na previously accumulated in roots appeared in the xylem exudate in 7 days after detopping. Na from the external medium, however, was readily transported to the exudate. Mor...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.50.3.388

    authors: Wallace A,Abou-Zamzam AM,Mueller RT

    更新日期:1972-09-01 00:00:00

  • Abnormal Stomatal Behavior and Hormonal Imbalance in flacca, a Wilty Mutant of Tomato: IV. Effect of Abscisic Acid and Water Content on RNase Activity and RNA.

    abstract::Plants of the wilty tomato (Lycopersicum esculentum) mutant, flacca, and of the normal cultivar Rheinlands Ruhm growing under either "normal" or high humidity were used in this research. Under normal humidity, RNase activity was much higher in mutant plants in which abscisic acid (ABA) and water content were lower tha...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.59.2.173

    authors: Puri J,Tal M

    更新日期:1977-02-01 00:00:00

  • Electrochemical aging responses in pisum: cellular adaptations or recovery from injury?

    abstract::Following excision, etiolated epicotyl segments of Pisum sativum L. cv. Alaska exhibit a marked hyperpolarization of membrane potential which is followed by a linear accumulation of K(+) when segments are incubated in Higinbotham nutrient solution. Segments aged for several hours and then reexcised display only a slig...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.67.4.864

    authors: Pierce WS

    更新日期:1981-04-01 00:00:00

  • Purification of the Major Soybean Leaf Acid Phosphatase That Is Increased by Seed-Pod Removal.

    abstract::Fruit removal for 5 weeks after flowering increased acid phosphatase activity 10-fold in soybean (Glycine max L. Merr. Var Hobbit) leaves compared with normal seed-pod-bearing plants. The major acid phosphatase activity in leaves was purified over 2700-fold, yielding a single polypeptide of 51 kD with a specific activ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.104.1.49

    authors: Staswick PE,Papa C,Huang JF,Rhee Y

    更新日期:1994-01-01 00:00:00

  • Assessment of cytoplasmic contaminations in isolated vacuole preparations.

    abstract::A method for testing the purity of isolated vacuoles is proposed. Preparations of isolated vacuoles often contain "impure vacuoles," namely, vacuoles contaminated with some cytoplasmic remnants. Such impure vacuoles are stained by the vital fluorescent dye fluorescein-diacetate. Vacuoles enclosed only by the tonoplast...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.65.1.85

    authors: Admon A,Jacoby B

    更新日期:1980-01-01 00:00:00

  • A new 1-aminocyclopropane-1-carboxylic acid-conjugating activity in tomato fruit.

    abstract::A new conjugate, 1-(gamma-L-glutamylamino)cyclopropane-1-carboxylic acid (GACC), of the ethylene precursor 1-aminocyclopropane-1-carboxylic acid (ACC) is identified. The only previously identified conjugate of ACC is 1-(malonylamino)cyclopropane-1-carboxylic acid (MACC). GACC, not MACC, was the major conjugate formed ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.109.3.917

    authors: Martin MN,Cohen JD,Saftner RA

    更新日期:1995-11-01 00:00:00

  • Induction and functional analysis of two reduced nicotinamide adenine dinucleotide phosphate-dependent glutathione peroxidase-like proteins in Synechocystis PCC 6803 during the progression of oxidative stress.

    abstract::Synechocystis PCC 6803 contains two types of glutathione peroxidase-like proteins (GPX-1 and GPX-2) that utilize NADPH but not reduced glutathione and unsaturated fatty acid hydroperoxides or alkyl hydroperoxides. The steady-state transcript level of gpx-1 gradually increased under oxidative stress conditions imposed ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.104.044842

    authors: Gaber A,Yoshimura K,Tamoi M,Takeda T,Nakano Y,Shigeoka S

    更新日期:2004-09-01 00:00:00

  • Elemental sulfur and thiol accumulation in tomato and defense against a fungal vascular pathogen.

    abstract::The occurrence of fungicidal, elemental S is well documented in certain specialized prokaryotes, but has rarely been detected in eukaryotes. Elemental S was first identified in this laboratory as a novel phytoalexin in the xylem of resistant genotypes of Theobroma cacao, after infection by the vascular, fungal pathoge...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:

    authors: Williams JS,Hall SA,Hawkesford MJ,Beale MH,Cooper RM

    更新日期:2002-01-01 00:00:00

  • Uptake of bicarbonate ion in darkness by isolated chloroplast envelope membranes and intact chloroplasts of spinach.

    abstract::Bicarbonate uptake by isolated chloroplast envelope membranes and intact chloroplasts of spinach (Spinacia oleracea L. var. Viroflay) in darkness exhibited a similar dependency upon temperature, pH, time, and concentrations of isolated or attached envelope membranes. This similarity in uptake properties demonstrates t...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.54.4.520

    authors: Poincelot RP

    更新日期:1974-10-01 00:00:00

  • Phytochrome modulation of blue light-induced chloroplast movements in Arabidopsis.

    abstract::Photometric analysis of chloroplast movements in various phytochrome (phy) mutants of Arabidopsis showed that phyA, B, and D are not required for chloroplast movements because blue light (BL)-dependent chloroplast migration still occurs in these mutants. However, mutants lacking phyA or phyB showed an enhanced respons...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.103.029116

    authors: DeBlasio SL,Mullen JL,Luesse DR,Hangarter RP

    更新日期:2003-12-01 00:00:00

  • EARLY FLOWERING 4 functions in phytochrome B-regulated seedling de-etiolation.

    abstract::To define the functions of genes previously identified by expression profiling as being rapidly light induced under phytochrome (phy) control, we are investigating the seedling de-etiolation phenotypes of mutants carrying T-DNA insertional disruptions at these loci. Mutants at one such locus displayed reduced responsi...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.103.030007

    authors: Khanna R,Kikis EA,Quail PH

    更新日期:2003-12-01 00:00:00

  • Plasma membrane potential of the alga dunaliella, and its relation to osmoregulation.

    abstract::A fluorescent dye sensitive to membrane potential was used to follow the plasma-membrane potential in the unicellular halo-tolerant alga Dunaliella salina. The signal observed during dissipation of the plasma membrane potential by the addition of excess K(+) and valinomycin, or a protonophore, was taken as a measure o...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.93.2.403

    authors: Oren-Shamir M,Pick U,Avron M

    更新日期:1990-06-01 00:00:00

  • Protein Synthesis in Imbibed Seeds III. Kinetics of Amino Acid Incorporation Ribosome Activation, and Polysome Formation.

    abstract::The kinetics of development of protein-synthesizing capacity in the imbibing wheat embryo, were studied both in vivo and in vitro. During the first 30 minutes of imbibition protein-synthesizing capacity rises rapidly, lagging about 10 minutes behind water uptake. This rise in synthesizing capacity is accompanied by an...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.41.7.1167

    authors: Marcus A,Feeley J,Volcani T

    更新日期:1966-09-01 00:00:00

  • Methyl-CpG-binding domain protein MBD7 is required for active DNA demethylation in Arabidopsis.

    abstract::Although researchers have established that DNA methylation and active demethylation are dynamically regulated in plant cells, the molecular mechanism for the regulation of active DNA demethylation is not well understood. By using an Arabidopsis (Arabidopsis thaliana) line expressing the Promoter RESPONSIVE TO DEHYDRAT...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.114.252106

    authors: Wang C,Dong X,Jin D,Zhao Y,Xie S,Li X,He X,Lang Z,Lai J,Zhu JK,Gong Z

    更新日期:2015-03-01 00:00:00

  • Signal transduction in maize and Arabidopsis mesophyll protoplasts.

    abstract::Plant protoplasts show physiological perceptions and responses to hormones, metabolites, environmental cues, and pathogen-derived elicitors, similar to cell-autonomous responses in intact tissues and plants. The development of defined protoplast transient expression systems for high-throughput screening and systematic...

    journal_title:Plant physiology

    pub_type: 杂志文章,评审

    doi:

    authors: Sheen J

    更新日期:2001-12-01 00:00:00

  • Inhibitors from Carob (Ceratonia siliqua L.): II. Effect on Growth Induced by Indoleacetic Acid or Gibberellins A(1), A(4), A(5), and A(7).

    abstract::Two inhibitory fractions (B(1) and C) from extracts of immature fruit of carob were tested for their ability to inhibit the action of indoleacetic acid (IAA) in three bioassays. There was no reduction of IAA-induced reactions in the Avena curvature test, abscission of debladed coleus petioles, or growth of cucumber hy...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.46.4.531

    authors: Corcoran MR

    更新日期:1970-10-01 00:00:00

  • Salinity stress inhibits bean leaf expansion by reducing turgor, not wall extensibility.

    abstract::Treatment of bean (Phaseolus vulgaris L.) seedlings with low levels of salinity (50 or 100 millimolar NaCl) decreased the rate of light-induced leaf cell expansion in the primary leaves over a 3 day period. This decrease could be due to a reduction in one or both of the primary cellular growth parameters: wall exten...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.88.1.233

    authors: Neumann PM,Van Volkenburgh E,Cleland RE

    更新日期:1988-01-01 00:00:00