Assessment of cytoplasmic contaminations in isolated vacuole preparations.

Abstract:

:A method for testing the purity of isolated vacuoles is proposed. Preparations of isolated vacuoles often contain "impure vacuoles," namely, vacuoles contaminated with some cytoplasmic remnants. Such impure vacuoles are stained by the vital fluorescent dye fluorescein-diacetate. Vacuoles enclosed only by the tonoplast, "naked vacuoles," do not stain.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Admon A,Jacoby B

doi

10.1104/pp.65.1.85

subject

Has Abstract

pub_date

1980-01-01 00:00:00

pages

85-7

issue

1

eissn

0032-0889

issn

1532-2548

journal_volume

65

pub_type

杂志文章
  • A simple theory regarding ambimobility of xenobiotics with special reference to the nematicide, oxamyl.

    abstract::A theory is presented to explain the phloem mobility of certain systemic xenobiotics that are not weak acids. It is shown that there is a theoretically optimum permeability that permits optimum circulation through the symplasm and apoplast (including the phloem and xylem) of Solanum tuberosum plants. The optimum perme...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.63.2.367

    authors: Tyree MT

    更新日期:1979-02-01 00:00:00

  • Oxygen effect on photosynthetic and glycolate pathways in young maize leaves.

    abstract::To study the effect of O(2) on the photosynthetic and glycolate pathways, maize leaves were exposed to (14)CO(2) during steady-state photosynthesis in 21 or 1% O(2). At the two O(2) concentrations after a (14)CO(2) pulse (4 seconds) followed by a (12)CO(2) chase, there was a slight difference in CO(2) uptake and in th...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.66.6.1079

    authors: Morot-Gaudry JF

    更新日期:1980-12-01 00:00:00

  • Protein Synthesis in Imbibed Seeds III. Kinetics of Amino Acid Incorporation Ribosome Activation, and Polysome Formation.

    abstract::The kinetics of development of protein-synthesizing capacity in the imbibing wheat embryo, were studied both in vivo and in vitro. During the first 30 minutes of imbibition protein-synthesizing capacity rises rapidly, lagging about 10 minutes behind water uptake. This rise in synthesizing capacity is accompanied by an...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.41.7.1167

    authors: Marcus A,Feeley J,Volcani T

    更新日期:1966-09-01 00:00:00

  • Specificity of hydrolysis of phytic acid by alkaline phytase from lily pollen.

    abstract::Phytases are the primary enzymes responsible for the hydrolysis of phytic acid, myo-inositol-1,2,3,4,5,6-hexakisphosphate (I-1,2,3,4,5,6-P6). A number of phytases with varying specificities, properties, and localizations hydrolyze phytic acid present in cells. The specificity of hydrolysis of phytic acid by alkaline p...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.106.4.1489

    authors: Barrientos L,Scott JJ,Murthy PP

    更新日期:1994-12-01 00:00:00

  • Use of the pressure vessel to measure concentrations of solutes in apoplastic and membrane-filtered symplastic sap in sunflower leaves.

    abstract::A simple, repeatable, and accurate method is described for the collection of apoplastic and membrane-filtered symplastic sap fractions, and for the determination of the origin of these fractions within the leaf. The apoplastic distribution patterns of the naturally occurring apoplastic leaf solutes, and the apoplastic...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.82.4.995

    authors: Jachetta JJ,Appleby AP,Boersma L

    更新日期:1986-12-01 00:00:00

  • Global gene profiling of laser-captured pollen mother cells indicates molecular pathways and gene subfamilies involved in rice meiosis.

    abstract::Pollen mother cells (PMCs) represent a critical early stage in plant sexual reproduction in which the stage is set for male gamete formation. Understanding the global molecular genetics of this early meiotic stage has so far been limited to whole stamen or floret transcriptome studies, but since PMCs are a discrete po...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.110.161661

    authors: Tang X,Zhang ZY,Zhang WJ,Zhao XM,Li X,Zhang D,Liu QQ,Tang WH

    更新日期:2010-12-01 00:00:00

  • The Liverwort, Marchantia, Drives Alternative Electron Flow Using a Flavodiiron Protein to Protect PSI.

    abstract::The diffusion efficiency of oxygen in the atmosphere, like that of CO2, is approximately 104 times greater than that in aqueous environments. Consequently, terrestrial photosynthetic organisms need mechanisms to protect against potential oxidative damage. The liverwort Marchantia polymorpha, a basal land plant, has ha...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.16.01038

    authors: Shimakawa G,Ishizaki K,Tsukamoto S,Tanaka M,Sejima T,Miyake C

    更新日期:2017-03-01 00:00:00

  • Role of asparagine in the photorespiratory nitrogen metabolism of pea leaves.

    abstract::In pea leaves, much of the metabolism of imported asparagine is by transamination. This activity was previously shown to be localized in the peroxisomes, suggesting a possible connection between asparagine and photorespiratory nitrogen metabolism. This was investigated by examination of the transfer of (15)N from the ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.78.2.334

    authors: Ta TC,Joy KW,Ireland RJ

    更新日期:1985-06-01 00:00:00

  • Rhythmicity in ethylene production in cotton seedlings.

    abstract::Cotyledons of cotton (Gossypium hirsutum L.) seedlings grown under a photoperiod of 12 hour darkness and 12 hour light showed daily oscillations in ethylene evolution. The rate of ethylene evolution began to increase toward the end of the dark period and reached a maximum rate during the first third of the light perio...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.75.2.493

    authors: Rikin A,Chalutz E,Anderson JD

    更新日期:1984-06-01 00:00:00

  • Identification of Genes Encoding Enzymes Catalyzing the Early Steps of Carrot Polyacetylene Biosynthesis.

    abstract::Polyacetylenic lipids accumulate in various Apiaceae species after pathogen attack, suggesting that these compounds are naturally occurring pesticides and potentially valuable resources for crop improvement. These compounds also promote human health and slow tumor growth. Even though polyacetylenic lipids were discove...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.18.01195

    authors: Busta L,Yim WC,LaBrant EW,Wang P,Grimes L,Malyszka K,Cushman JC,Santos P,Kosma DK,Cahoon EB

    更新日期:2018-12-01 00:00:00

  • Tailoring the specificity of a plant cystatin toward herbivorous insect digestive cysteine proteases by single mutations at positively selected amino acid sites.

    abstract::Plant cystatins, similar to other defense proteins, include hypervariable, positively selected amino acid sites presumably impacting their biological activity. Using 29 single mutants of the eighth domain of tomato (Solanum lycopersicum) multicystatin, SlCYS8, we assessed here the potential of site-directed mutagenesi...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.108.115741

    authors: Goulet MC,Dallaire C,Vaillancourt LP,Khalf M,Badri AM,Preradov A,Duceppe MO,Goulet C,Cloutier C,Michaud D

    更新日期:2008-03-01 00:00:00

  • Nucleoid-enriched proteomes in developing plastids and chloroplasts from maize leaves: a new conceptual framework for nucleoid functions.

    abstract::Plastids contain multiple copies of the plastid chromosome, folded together with proteins and RNA into nucleoids. The degree to which components of the plastid gene expression and protein biogenesis machineries are nucleoid associated, and the factors involved in plastid DNA organization, repair, and replication, are ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.111.188474

    authors: Majeran W,Friso G,Asakura Y,Qu X,Huang M,Ponnala L,Watkins KP,Barkan A,van Wijk KJ

    更新日期:2012-01-01 00:00:00

  • Effects of Previous Pollination and Stylar Ethylene on Pollen Tube Growth in Petunia hybrida Styles.

    abstract::The effect of ethylene on the growth rate of pollen tubes in styles of Petunia hybrida was examined. Apart from its strong inhibition of pollination-induced ethylene synthesis, aminoethoxyvinylglycine, placed on the stigma, did not impede tube growth. The inhibitors of the action of ethylene, silver thiosulfate and 2,...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.86.1.4

    authors: Hoekstra FA,van Roekel T

    更新日期:1988-01-01 00:00:00

  • Dissociation of polysome aggregates by protease k.

    abstract::Apparent large size-classes of zein-synthesizing polysomes from developing kernels of Zea mays L. were converted to smaller polysomes after treatment with Protease K. The reduction in polysome size was not a result of ribonuclease activity, inasmuch as the enzyme did not affect the free polysomes or the size of the mR...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.60.4.482

    authors: Larkins BA,Tsai CY

    更新日期:1977-10-01 00:00:00

  • Regulatory Phosphorylation of C4 Phosphoenolpyruvate Carboxylase (A Cardinal Event Influencing the Photosynthesis Rate in Sorghum and Maize).

    abstract::C4 leaf phosphoenolpyruvate carboxylase (PEPC; EC 4.1.1.31) is subject to a day/night regulatory phosphorylation cycle. By using the cytoplasmic protein synthesis inhibitor cycloheximide (CHX), we previously reported that the reversible in vivo light activation of the C4 PEPC protein-serine kinase requires protein syn...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.101.3.891

    authors: Bakrim N,Prioul JL,Deleens E,Rocher JP,Arrio-Dupont M,Vidal J,Gadal P,Chollet R

    更新日期:1993-03-01 00:00:00

  • Involvement of Singlet Oxygen in 5-Aminolevulinic Acid-Induced Photodynamic Damage of Cucumber (Cucumis sativus L.) Chloroplasts.

    abstract::Cucumber (Cucumis sativus L., cv Poinsette) plants were sprayed with 20 millimolar 5-aminolevulinic acid and then incubated in the dark for 14 hours. The intact chloroplasts were isolated from the above plants in the dark and were exposed to weak light (250 micromoles per square meter per second). Within 30 minutes, p...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.98.1.7

    authors: Chakraborty N,Tripathy BC

    更新日期:1992-01-01 00:00:00

  • Functional conservation of PISTILLATA activity in a pea homolog lacking the PI motif.

    abstract::Current understanding of floral development is mainly based on what we know from Arabidopsis (Arabidopsis thaliana) and Antirrhinum majus. However, we can learn more by comparing developmental mechanisms that may explain morphological differences between species. A good example comes from the analysis of genes control...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.104.057687

    authors: Berbel A,Navarro C,Ferrándiz C,Cañas LA,Beltrán JP,Madueño F

    更新日期:2005-09-01 00:00:00

  • Regulation of Photosynthetic Rate of Two Sunflower Hybrids under Water Stress.

    abstract::The effect of short-term water stress on photosynthesis of two sunflower hybrids (Helianthus annuus L. cv Sungro-380 and cv SH-3622), differing in productivity under field conditions, was measured. The rate of CO(2) assimilation of young, mature leaves of SH-3622 under well-watered conditions was approximately 30% gre...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.98.2.516

    authors: Gimenez C,Mitchell VJ,Lawlor DW

    更新日期:1992-02-01 00:00:00

  • Bypassing Iron Storage in Endodermal Vacuoles Rescues the Iron Mobilization Defect in the natural resistance associated-macrophage protein3natural resistance associated-macrophage protein4 Double Mutant.

    abstract::To improve seed iron (Fe) content and bioavailability, it is crucial to decipher the mechanisms that control Fe storage during seed development. In Arabidopsis (Arabidopsis thaliana) seeds, most Fe is concentrated in insoluble precipitates, with phytate in the vacuoles of cells surrounding the vasculature of the embry...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.15.00380

    authors: Mary V,Schnell Ramos M,Gillet C,Socha AL,Giraudat J,Agorio A,Merlot S,Clairet C,Kim SA,Punshon T,Guerinot ML,Thomine S

    更新日期:2015-09-01 00:00:00

  • The role of pyruvate dehydrogenase and acetyl-coenzyme A synthetase in fatty acid synthesis in developing Arabidopsis seeds.

    abstract::Acetyl-coenzyme A (acetyl-CoA) formed within the plastid is the precursor for the biosynthesis of fatty acids and, through them, a range of important biomolecules. The source of acetyl-CoA in the plastid is not known, but two enzymes are thought to be involved: acetyl-CoA synthetase and plastidic pyruvate dehydrogenas...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.123.2.497

    authors: Ke J,Behal RH,Back SL,Nikolau BJ,Wurtele ES,Oliver DJ

    更新日期:2000-06-01 00:00:00

  • Effects of Nitrate Application on Amaranthus powellii Wats : II. Stomatal Response to Vapor Pressure Difference is Consistent with Optimization of Stomatal Conductance.

    abstract::The hypothesis of optimal stomatal conductance predicts conductance should vary with changes of the vapor pressure difference between leaf and air (VPD) to keep the partial derivative of transpiration rate (E) with respect to assimilation rate (A) constant ( partial differentialE/ partial differentialA = lambda). Stom...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.79.3.614

    authors: Hunt ER,Weber JA,Gates DM

    更新日期:1985-11-01 00:00:00

  • C Tracer Evidence for Synthesis of Choline and Betaine via Phosphoryl Base Intermediates in Salinized Sugarbeet Leaves.

    abstract::Like other chenopods, sugarbeets (Beta vulgaris L. cv Great Western D-2) accumulate glycine betaine when salinized; this may be an adaptive response to stress. The pathway of betaine synthesis in leaves of salinized (150-200 millimolar NaCl) sugarbeet plants was investigated by supplying [(14)C]formate, phosphoryl[(14...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.71.3.692

    authors: Hanson AD,Rhodes D

    更新日期:1983-03-01 00:00:00

  • Phospholipids in the Uredospores of Uromyces phaseoli: II. Metabolism during Germination.

    abstract::The levels and types of phospholipids changed in distinct phases during the germination of (32)P-labeled uredospores of Uromyces phaseoli. During the first 20 minutes of germination, the phospholipid content dropped to 40% of the pregermination level. Between 2 and 3 hours, phospholipid levels increased to approximate...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.48.6.735

    authors: Langenbach RJ,Knoche HW

    更新日期:1971-12-01 00:00:00

  • Purification and characterization of mitochondrial citrate synthase.

    abstract::Mitochondrial citrate synthase was purified from leaves of Pisum sativum L. cv Progress 9. A three step purification was employed using ATP-Sepharose affinity chromatography which resulted in a 600-fold enrichment. Enzyme activity was assayed spectrophotometrically during greening of etiolated leaves under constant wh...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.89.3.719

    authors: Unger EA,Vasconcelos AC

    更新日期:1989-03-01 00:00:00

  • CO(2) Enhancement of Growth and Photosynthesis in Rice (Oryza sativa) : Modification by Increased Ultraviolet-B Radiation.

    abstract::Two cultivars of rice (Oryza sativa L.) IR-36 and Fujiyama-5 were grown at ambient (360 microbars) and elevated CO(2) (660 microbars) from germination through reproduction in unshaded greenhouses at the Duke University Phytotron. Growth at elevated CO(2) resulted in significant decreases in nighttime respiration and i...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.99.2.473

    authors: Ziska LH,Teramura AH

    更新日期:1992-06-01 00:00:00

  • Ectopic expression of a horseradish peroxidase enhances growth rate and increases oxidative stress resistance in hybrid aspen.

    abstract::We previously demonstrated that overexpression of the horseradish (Armoracia rusticana) peroxidase prxC1a gene stimulated the growth rate of tobacco (Nicotiana tabacum) plants. Here, the cauliflower mosaic virus 35S::prxC1a construct was introduced into hybrid aspen (Populus sieboldii x Populus grandidentata). The gro...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.102.019794

    authors: Kawaoka A,Matsunaga E,Endo S,Kondo S,Yoshida K,Shinmyo A,Ebinuma H

    更新日期:2003-07-01 00:00:00

  • TAP46 plays a positive role in the ABSCISIC ACID INSENSITIVE5-regulated gene expression in Arabidopsis.

    abstract::TAP46 is a protein phosphatase2A (PP2A)-associated protein that regulates PP2A activity in Arabidopsis (Arabidopsis thaliana). To study how PP2A is involved in abscisic acid (ABA) signaling in plants, we studied the function of TAP46 in ABA-regulated seed maturation and seedling development. Expression of TAP46 coinci...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.113.233684

    authors: Hu R,Zhu Y,Shen G,Zhang H

    更新日期:2014-02-01 00:00:00

  • Sucrose phosphate synthase, a key enzyme for sucrose biosynthesis in plants: protein purification from corn leaves and immunological detection.

    abstract::We have purified the protein for the enzyme sucrose phosphate synthase (SPS) from corn (Zea mays) leaves. Partially purified SPS protein was used to generate specific monoclonal antibodies. The following immunoaffinity chromatography allowed the isolation of pure SPS protein. The apparent molecular mass of the SPS pol...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.96.2.473

    authors: Bruneau JM,Worrell AC,Cambou B,Lando D,Voelker TA

    更新日期:1991-06-01 00:00:00

  • Inhibition of Flowering of Xanthium pensylvanicum Wallr. by Prolonged Irradiation with Far Red.

    abstract::Interrupting each long night with a prolonged period of far red radiant energy resulted in the inhibition of floral initiation in cocklebur. Irradiations inducing different relative levels of P(FR) from 1 to 2% to 80% had about the same effect under 4-hour photoperiods. The lower levels of P(FR) induced by continuous ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.42.1.95

    authors: Mancinelli AL,Downs RJ

    更新日期:1967-01-01 00:00:00

  • Evidence for osmotic regulation of hydrolytic enzyme production in germinating barley seeds.

    abstract::alpha-Amylase levels in intact seeds of barley (Hordeum vulgare L. cv. Himalaya) reach a maximum at 3 to 4 days of germination while gibberellin levels continue to increase beyond 6 days of germination. In contrast to its effect on half seeds, gibberellic acid does not increase the total amount of alpha-amylase produc...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.48.2.137

    authors: Jones RL,Armstrong JE

    更新日期:1971-08-01 00:00:00