Abstract:
:Apparent large size-classes of zein-synthesizing polysomes from developing kernels of Zea mays L. were converted to smaller polysomes after treatment with Protease K. The reduction in polysome size was not a result of ribonuclease activity, inasmuch as the enzyme did not affect the free polysomes or the size of the mRNA from the membrane-bound polysomes. High concentrations of MgCl(2) in polysome buffer inhibited ribonuclease activity and appeared to cause protein interaction between nascent zein polypeptides. Although Protease K inhibited the polysome's capacity for protein synthesis, it was a useful reagent for determining if polysomes were aggregated by protein.
journal_name
Plant Physioljournal_title
Plant physiologyauthors
Larkins BA,Tsai CYdoi
10.1104/pp.60.4.482subject
Has Abstractpub_date
1977-10-01 00:00:00pages
482-5issue
4eissn
0032-0889issn
1532-2548journal_volume
60pub_type
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