Ethylene production by auxin-deprived, suspension-cultured pear fruit cells in response to auxins, stress, or precursor.

Abstract:

:Auxin-deprived, mannitol-supplemented, suspension-cultured pear (Pyrus communis L. Passe Crassane) fruit cells produce large quantities (20-40 nanoliters ethylene per 10(6) cells per hour) of ethylene in response to auxins, CuCl(2) or 1-amino-cyclopropane-1-carboxylic acid (ACC). Maximum rates of production are achieved about 12 hours after the addition of optimal amounts of indoleacetic acid (IAA), naphthalene acetic acid (NAA), 2,4-dichlorophenoxyacetic acid (2,4-D), 4 to 5 hours after the addition of CuCl(2) and 1 to 2 hours after the addition of ACC. Supraoptimal concentrations of IAA result in a lag phase followed by a normal response. High concentrations of NAA and 2,4-D result in an early (4-5 hours) stress response and injury.Continuous protein and RNA synthesis are essential for elaboration of the full IAA response; only protein synthesis is necessary for the response to CuCl(2) and ACC. Based on polysomal states and rates of amino acid incorporation, CuCl(2) partially inhibits protein synthesis while nonetheless stimulating ethylene production. In general, ethylene production by the pear cells resembles that of other plant systems. Some differences may reflect the sensitivity of the cells and are discussed. The relatively high levels of ethylene produced and the experimental convenience of the cultured cells should make them especially suitable for further investigations of ethylene production and physiology.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Puschmann R,Romani R

doi

10.1104/pp.73.4.1013

subject

Has Abstract

pub_date

1983-12-01 00:00:00

pages

1013-9

issue

4

eissn

0032-0889

issn

1532-2548

journal_volume

73

pub_type

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