Dead or Alive? Using Membrane Failure and Chlorophyll a Fluorescence to Predict Plant Mortality from Drought.

Abstract:

:Climate models predict widespread increases in both drought intensity and duration in the next decades. Although water deficiency is a significant determinant of plant survival, limited understanding of plant responses to extreme drought impedes forecasts of both forest and crop productivity under increasing aridity. Drought induces a suite of physiological responses; however, we lack an accurate mechanistic description of plant response to lethal drought that would improve predictive understanding of mortality under altered climate conditions. Here, proxies for leaf cellular damage, chlorophyll a fluorescence, and electrolyte leakage were directly associated with failure to recover from drought upon rewatering in Brassicarapa (genotype R500) and thus define the exact timing of drought-induced death. We validated our results using a second genotype (imb211) that differs substantially in life history traits. Our study demonstrates that whereas changes in carbon dynamics and water transport are critical indicators of drought stress, they can be unrelated to visible metrics of mortality, i.e. lack of meristematic activity and regrowth. In contrast, membrane failure at the cellular scale is the most proximate cause of death. This hypothesis was corroborated in two gymnosperms (Picea engelmannii and Pinus contorta) that experienced lethal water stress in the field and in laboratory conditions. We suggest that measurement of chlorophyll a fluorescence can be used to operationally define plant death arising from drought, and improved plant characterization can enhance surface model predictions of drought mortality and its consequences to ecosystem services at a global scale.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Guadagno CR,Ewers BE,Speckman HN,Aston TL,Huhn BJ,DeVore SB,Ladwig JT,Strawn RN,Weinig C

doi

10.1104/pp.16.00581

subject

Has Abstract

pub_date

2017-09-01 00:00:00

pages

223-234

issue

1

eissn

0032-0889

issn

1532-2548

pii

pp.16.00581

journal_volume

175

pub_type

杂志文章
  • The rapid isolation of vacuoles from leaves of crassulacean Acid metabolism plants.

    abstract::A technique is presented for the isolation of vacuoles from Sedum telephium L. leaves. Leaf material is digested enzymically to produce protoplasts rapidly which are partially lysed by gentle osmotic shock and the inclusion of 5 millimolar ethyleneglycol-bis (beta-aminoethyl ether)N,N'-tetraacetic acid in the wash med...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.66.3.379

    authors: Kringstad R,Kenyon WH,Black CC

    更新日期:1980-09-01 00:00:00

  • Efficient Plastid Transformation in Arabidopsis.

    abstract::Plastid transformation is routine in tobacco (Nicotiana tabacum) but 100-fold less frequent in Arabidopsis (Arabidopsis thaliana), preventing its use in plastid biology. A recent study revealed that null mutations in ACC2, encoding a plastid-targeted acetyl-coenzyme A carboxylase, cause hypersensitivity to spectinomyc...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.17.00857

    authors: Yu Q,Lutz KA,Maliga P

    更新日期:2017-09-01 00:00:00

  • Root Type-Specific Reprogramming of Maize Pericycle Transcriptomes by Local High Nitrate Results in Disparate Lateral Root Branching Patterns.

    abstract::The adaptability of root system architecture to unevenly distributed mineral nutrients in soil is a key determinant of plant performance. The molecular mechanisms underlying nitrate dependent plasticity of lateral root branching across the different root types of maize are only poorly understood. In this study, detail...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.15.01885

    authors: Yu P,Baldauf JA,Lithio A,Marcon C,Nettleton D,Li C,Hochholdinger F

    更新日期:2016-03-01 00:00:00

  • Iron uptake and translocation by tomato plants as influenced by root temperature and manganese nutrition.

    abstract::The uptake of iron (Fe) by VF-36 tomato plants as influenced by root temperature and manganese (Mn) nutrition was studied. Following a 1-week pretreatment period of various levels of Fe and Mn in the nutrient solution in a controlled temperature room, the uptake of (59)Fe from ferric ethylenediamine di(O-hydroxyphenyl...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.41.7.1095

    authors: Riekels JW,Lingle JC

    更新日期:1966-09-01 00:00:00

  • The Translesion Polymerase ζ Has Roles Dependent on and Independent of the Nuclease MUS81 and the Helicase RECQ4A in DNA Damage Repair in Arabidopsis.

    abstract::DNA polymerase zeta catalytic subunit REV3 is known to play an important role in the repair of DNA damage induced by cross-linking and methylating agents. Here, we demonstrate that in Arabidopsis (Arabidopsis thaliana), the basic polymerase activity of REV3 is essential for resistance protection against these differen...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.15.00806

    authors: Kobbe S,Trapp O,Knoll A,Manuss A,Puchta H

    更新日期:2015-12-01 00:00:00

  • Relative activities of NAD- and NADP- isocritric dehydrogenases in bean mitochondria modified by glycerol or NADP.

    abstract::Mitochondria from cotyledons of Vigna sesquipedalis (L.) Fruwirth (starchy seed) showed no NAD-isocitric dehydrogenase (NAD-IDH) activity by the methods which have been known to be useful for the detection of NAD-IDH in mitochondria of plants including castor bean and alaska pea. When the Vigna cotyledon mitochondria ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.44.2.262

    authors: Yamamoto Y

    更新日期:1969-02-01 00:00:00

  • Effect of aminoethoxy analog of rhizobitoxine on ripening of pears.

    abstract::Ripening reactions in pears (Pyrus communis L.) were differentially affected by an aminoethoxy analog of rhizobitoxine (l-2-amino-4-[2-aminoethoxy]-trans-3-butenoic acid) (AAR). Ethylene production of both ;Anjou' and ;Bartlett' pears was inhibited by AAR. Decrease in firmness, increase in protein N and soluble pectin...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.59.4.546

    authors: Wang CY,Mellenthin WM

    更新日期:1977-04-01 00:00:00

  • An Improved Brome mosaic virus Silencing Vector: Greater Insert Stability and More Extensive VIGS.

    abstract::Virus-induced gene silencing (VIGS) is used extensively for gene function studies in plants. VIGS is inexpensive and rapid compared with silencing conducted through stable transformation, but many virus-silencing vectors, especially in grasses, induce only transient silencing phenotypes. A major reason for transient p...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.17.00905

    authors: Ding XS,Mannas SW,Bishop BA,Rao X,Lecoultre M,Kwon S,Nelson RS

    更新日期:2018-01-01 00:00:00

  • Permeability and channel-mediated transport of boric acid across membrane vesicles isolated from squash roots.

    abstract::Boron is an essential micronutrient for plant growth and the boron content of plants differs greatly, but the mechanism(s) of its uptake into cells is not known. Boron is present in the soil solution as boric acid and it is in this form that it enters the roots. We determined the boron permeability coefficient of puri...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.124.3.1349

    authors: Dordas C,Chrispeels MJ,Brown PH

    更新日期:2000-11-01 00:00:00

  • Cotyledon-Generated Auxin Is Required for Shade-Induced Hypocotyl Growth in Brassica rapa.

    abstract::Plant architecture is optimized for the local light environment. In response to foliar shade or neighbor proximity (low red to far-red light), some plant species exhibit shade-avoiding phenotypes, including increased stem and hypocotyl growth, which increases the likelihood of outgrowing competitor plants. If shade pe...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.114.241844

    authors: Procko C,Crenshaw CM,Ljung K,Noel JP,Chory J

    更新日期:2014-07-01 00:00:00

  • Function of Rhizodermal Transfer Cells in the Fe Stress Response Mechanism of Capsicum annuum L.

    abstract::A variety of red pepper (Capsicum annuum L., cv Yaglik) responds to Fe deficiency stress with simultaneously enhanced H(+) extrusion, reduction of ferric ions and synthesis of malic and citric acid in a swollen subapical root zone densely covered with root hairs. It is demonstrated that these stress responses temporal...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.82.2.511

    authors: Landsberg EC

    更新日期:1986-10-01 00:00:00

  • Synergistic biosynthesis of biphasic ethylene and reactive oxygen species in response to hemibiotrophic Phytophthora parasitica in tobacco plants.

    abstract::We observed the biphasic production of ethylene and reactive oxygen species (ROS) in susceptible tobacco (Nicotiana tabacum 'Wisconsin 38') plants after shoot inoculation with Phytophthora parasitica var nicotianae. The initial transient increase in ROS and ethylene at 1 and 3 h (phase I), respectively, was followed b...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.112.194654

    authors: Wi SJ,Ji NR,Park KY

    更新日期:2012-05-01 00:00:00

  • Calcium-Dependent Hydrogen Peroxide Mediates Hydrogen-Rich Water-Reduced Cadmium Uptake in Plant Roots.

    abstract::Hydrogen gas (H2) has a possible signaling role in many developmental and adaptive plant responses, including mitigating the harmful effects of cadmium (Cd) uptake from soil. We used electrophysiological and molecular approaches to understand how H2 ameliorates Cd toxicity in pak choi (Brassicacampestris ssp. chinensi...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.20.00377

    authors: Wu Q,Huang L,Su N,Shabala L,Wang H,Huang X,Wen R,Yu M,Cui J,Shabala S

    更新日期:2020-07-01 00:00:00

  • Root formation in ethylene-insensitive plants.

    abstract::Experiments with ethylene-insensitive tomato (Lycopersicon esculentum) and petunia (Petunia x hybrida) plants were conducted to determine if normal or adventitious root formation is affected by ethylene insensitivity. Ethylene-insensitive Never ripe (NR) tomato plants produced more below-ground root mass but fewer abo...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.121.1.53

    authors: Clark DG,Gubrium EK,Barrett JE,Nell TA,Klee HJ

    更新日期:1999-09-01 00:00:00

  • Tonoplast stability and survival of isolated vacuoles in different buffers.

    abstract::The mechanism of sucrose transport into vacuoles isolated from leaf tissue has been studied only in barley (Hordeum vulgare) mesophyll cells. In this tissue, sucrose transport was reported to be a facilitated diffusion. We have observed a facilitated diffusion of sucrose into vacuoles isolated from this tissue. Howeve...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.88.2.251

    authors: De Leon JL,Daie J,Wyse R

    更新日期:1988-10-01 00:00:00

  • Early effects of salinity on nitrate assimilation in barley seedlings.

    abstract::The effect of NaCl and Na(2)SO(4) salinity on NO(3) (-) assimilation in young barley (Hordeum vulgare L. var Numar) seedlings was studied. The induction of the NO(3) (-) transporter was affected very little; the major effect of the salts was on its activity. Both Cl(-) and SO(4) (2-) salts severely inhibited uptake of...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.76.2.321

    authors: Aslam M,Huffaker RC,Rains DW

    更新日期:1984-10-01 00:00:00

  • Continuous turnover of carotenes and chlorophyll a in mature leaves of Arabidopsis revealed by 14CO2 pulse-chase labeling.

    abstract::Carotenoid turnover was investigated in mature leaves of Arabidopsis (Arabidopsis thaliana) by 14CO2 pulse-chase labeling under control-light (CL; 130 micromol photons m(-2) s(-1)) and high-light (HL; 1,000 micromol photons m(-2) s(-1)) conditions. Following a 30-min 14CO2 administration, photosynthetically fixed 14C ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.109.151647

    authors: Beisel KG,Jahnke S,Hofmann D,Köppchen S,Schurr U,Matsubara S

    更新日期:2010-04-01 00:00:00

  • Contribution of different carbon sources to isoprene biosynthesis in poplar leaves.

    abstract::This study was performed to test if alternative carbon sources besides recently photosynthetically fixed CO2 are used for isoprene formation in the leaves of young poplar (Populus x canescens) trees. In a 13CO2 atmosphere under steady state conditions, only about 75% of isoprene became 13C labeled within minutes. A co...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.103.037374

    authors: Schnitzler JP,Graus M,Kreuzwieser J,Heizmann U,Rennenberg H,Wisthaler A,Hansel A

    更新日期:2004-05-01 00:00:00

  • Integrated Analysis of Engineered Carbon Limitation in a Quadruple CO2/HCO3- Uptake Mutant of Synechocystis sp. PCC 6803.

    abstract::Cyanobacteria have efficient carbon concentration mechanisms and suppress photorespiration in response to inorganic carbon (Ci) limitation. We studied intracellular Ci limitation in the slow-growing CO2/HCO3 (-)-uptake mutant ΔndhD3 (for NADH dehydrogenase subunit D3)/ndhD4 (for NADH dehydrogenase subunit D4)/cmpA (fo...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.15.01289

    authors: Orf I,Klähn S,Schwarz D,Frank M,Hess WR,Hagemann M,Kopka J

    更新日期:2015-11-01 00:00:00

  • Specificity for Nicotinamide Adenine Dinucleotide and Nicotinamide Adenine Dinucleotide Phosphate of Nitrate Reductase from the Salt-tolerant Alga Dunaliella parva.

    abstract::Nitrate reductase of the salt-tolerant alga Dunaliella parva could utilize NADPH as well as NADH as an electron donor. The two pyridine nucleotide-dependent activities could not be separated by either ion exchange chromatography on DEAE-cellulose or gel filtration on Sepharose 4B. The NADPH-dependent activity was not ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.58.1.57

    authors: Heimer YM

    更新日期:1976-07-01 00:00:00

  • Compensation point and isotopic characteristics of c(3)/c(4) intermediates and hybrids in panicum.

    abstract::Leaf CO(2) compensation points and stable hydrogen, oxygen and carbon isotope ratios were determined for Panicum species including C(3)/C(4) intermediate photosynthesis plants, hybrids between C(3)/C(4) intermediates and C(3) plants, C(3) and C(4) plants in the Panicum genus as well as several other C(3) and C(4) plan...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.80.1.242

    authors: Sternberg Lda S,Deniro MJ,Sloan ME,Black CC

    更新日期:1986-01-01 00:00:00

  • Why leaves turn red in autumn. The role of anthocyanins in senescing leaves of red-osier dogwood.

    abstract::Why the leaves of many woody species accumulate anthocyanins prior to being shed has long puzzled biologists because it is unclear what effects anthocyanins may have on leaf function. Here, we provide evidence for red-osier dogwood (Cornus stolonifera) that anthocyanins form a pigment layer in the palisade mesophyll l...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:

    authors: Feild TS,Lee DW,Holbrook NM

    更新日期:2001-10-01 00:00:00

  • Knockout of a bacterial-type ATP-binding cassette transporter gene, AtSTAR1, results in increased aluminum sensitivity in Arabidopsis.

    abstract::ATP-binding cassette (ABC) transporters represent a large family in plants, but the functions of most of these transporters are unknown. Here we report a gene, AtSTAR1, only encoding an ATP-binding domain of a bacterial-type ABC transporter in Arabidopsis (Arabidopsis thaliana). AtSTAR1 is an ortholog of rice (Oryza s...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.110.155028

    authors: Huang CF,Yamaji N,Ma JF

    更新日期:2010-08-01 00:00:00

  • Clonal variation for tolerance to polyethylene glycol-induced water stress in cultured tomato cells.

    abstract::Cell clones were isolated from a population of cultured tomato (Lycopersicon esculentum Mill cv VFNT-cherry) cells and their tolerance to polyethylene glycol (PEG)-induced water stress was measured. Considerable variation for tolerance among the clones was found. Tolerance differences between clones appeared to be spo...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.72.3.645

    authors: Handa AK,Bressan RA,Handa S,Hasegawa PM

    更新日期:1983-07-01 00:00:00

  • Specific inhibition of the cyanide-insensitive respiratory pathway in plant mitochondria by hydroxamic acids.

    abstract::Hydroxamic acids, R-CONHOH, are inhibitors specific to the respiratory pathway through the alternate, cyanide-insensitive terminal oxidase of plant mitochondria. The nature of the R group in these compounds affects the concentration at which the hydroxamic acids are effective, but it appears that all hydroxamic acids ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.47.1.124

    authors: Schonbaum GR,Bonner WD Jr,Storey BT,Bahr JT

    更新日期:1971-01-01 00:00:00

  • A Single Arabidopsis Gene Encodes Two Differentially Targeted Geranylgeranyl Diphosphate Synthase Isoforms.

    abstract::A wide diversity of isoprenoids is produced in different plant compartments. Most groups of isoprenoids synthesized in plastids, and some produced elsewhere in the plant cell derive from geranylgeranyl diphosphate (GGPP) synthesized by GGPP synthase (GGPPS) enzymes. In Arabidopsis (Arabidopsis thaliana), five genes ap...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.16.01392

    authors: Ruiz-Sola MÁ,Barja MV,Manzano D,Llorente B,Schipper B,Beekwilder J,Rodriguez-Concepcion M

    更新日期:2016-11-01 00:00:00

  • The dominant mutant Wavy auricle in blade1 disrupts patterning in a lateral domain of the maize leaf.

    abstract::Mature maize leaves have defined cell types along the proximal distal and medial lateral axes. The patterning events that establish these axes take place early in leaf initiation. We have identified a new dominant mutation, Wavy auricle in blade1 (Wab1), which affects patterning in both axes in a dose-dependent manner...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.103.036707

    authors: Hay A,Hake S

    更新日期:2004-05-01 00:00:00

  • Nod factors induce nod factor cleaving enzymes in pea roots. Genetic and pharmacological approaches indicate different activation mechanisms.

    abstract::Establishment of symbiosis between legumes and rhizobia requires bacterial Nod factors (NFs). The concentration of these lipochitooligosaccharides in the rhizosphere is influenced by plant enzymes. NFs induce on pea (Pisum sativum) a particular extracellular NF hydrolase that releases lipodisaccharides from NFs from S...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.105.061705

    authors: Ovtsyna AO,Dolgikh EA,Kilanova AS,Tsyganov VE,Borisov AY,Tikhonovich IA,Staehelin C

    更新日期:2005-10-01 00:00:00

  • Thermoperiodism in cocklebur seed germination.

    abstract::Germination potential in nondormant, upper cocklebur (Xanthium pensylvanicum Wallr.) seeds, which were incapable of germinating under constant temperatures below 25 C in air, was increased by exposure to diurnally alternating temperatures. The cocklebur seeds failed to respond to the temperature fluctuations in the be...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.61.3.437

    authors: Esashi Y,Tsukada Y

    更新日期:1978-03-01 00:00:00

  • Water Relations and Growth of the flacca Tomato Mutant in Relation to Abscisic Acid.

    abstract::The flacca mutant in tomato (Lycopersicon esculentum Mill. cv Rheinlands Ruhm) was employed to examine the effects of a relatively constant diurnal water stress on leaf growth and water relations. As the mutant is deficient in abscisic acid (ABA) and can be phenotypically reverted to the wild type by applications of t...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.72.1.251

    authors: Bradford KJ

    更新日期:1983-05-01 00:00:00