Abstract:
:Data are presented which demonstrate a citrate cleavage enzyme in the supernatant of a developing soybean (Glycine max L. Merr., var. Harosoy 63) cotyledon homogenate following a 126,000g spin for 2 hours. Activity of the enzyme was observed directly in the supernatant enzyme preparation and in a desalted supernatant preparation by measuring the formation of acetylhydroxamate. Acetylhydroxamate production was dependent on citrate and coenzyme A. The reaction increased with time, citrate, and coenzyme A concentrations.Involvement of the enzyme in lipid synthesis was investigated by the incorporation of carbon from citrate-1,5-(14)C into fatty acids. Incorporation shows a pH optimum at 8.5, a temperature optimum at 30 C, and a dependence on ATP and coenzyme A. The reaction is linear throughout the range of extract concentrations tested and is linear as a function of time for 1 hour. Isotope was distributed primarily in unsaturated fatty acids.
journal_name
Plant Physioljournal_title
Plant physiologyauthors
Nelson DR,Rinne RWdoi
10.1104/pp.55.1.69subject
Has Abstractpub_date
1975-01-01 00:00:00pages
69-72issue
1eissn
0032-0889issn
1532-2548journal_volume
55pub_type
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