Use of the pressure vessel to measure concentrations of solutes in apoplastic and membrane-filtered symplastic sap in sunflower leaves.

abstract：:In all eukaryotes, cell cycle progression is controlled by cyclin-dependent kinases (CDKs) whose activity is regulated at several levels including inhibition by CDK inhibitors. Here, we report a comparative molecular and functional analysis of the tobacco (Nicotiana tomentosiformis) CDK inhibitor, NtKIS1a, and its spl...

journal_title：Plant physiology

authors： Jasinski S,Perennes C,Bergounioux C,Glab N

更新日期：2002-12-01 00:00:00

abstract：:Phytochrome A (phyA) is the primary photoreceptor mediating responses to far-red light. Among the phyA downstream signaling components, Far-red Elongated Hypocotyl 1 (FHY1) is a genetically defined positive regulator of photomorphogenesis in far-red light. Both physiological and genomic characterization of the fhy1 mu...

journal_title：Plant physiology

authors： Shen Y,Feng S,Ma L,Lin R,Qu LJ,Chen Z,Wang H,Deng XW

更新日期：2005-11-01 00:00:00

abstract：:Expression of a beta-amylase gene of Arabidopsis thaliana (AT beta-Amy) is regulated by sugars. We identified a mutant, hba1, in which the level of expression of AT beta-Amy in leaves of plants that had been grown in a medium with 2% sucrose was significantly higher than that in wild-type plants. Higher that wild-type...

journal_title：Plant physiology

authors： Mita S,Hirano H,Nakamura K

更新日期：1997-06-01 00:00:00

abstract：:Cell-free extracts of Chlorella pyrenoidosa contained two enzymes capable of oxidizing d-lactate; these were glycolate dehydrogenase and NAD(+)-dependent d-lactate dehydrogenase. The two enzymes could be distinguished by differential centrifugation, glycolate dehydrogenase being largely particulate and NAD(+)-d-lactat...

journal_title：Plant physiology

authors： Gruber PJ,Frederick SE,Tolbert NE

更新日期：1974-02-01 00:00:00

abstract：:Soybeans (Glycine max) exposed to chronic levels of ozone showed a linear decrease in biomass with increasing concentration. The foliar free sterols increased while the steryl ester, and the steryl glycosides, a minor component, decreased with increasing pollutant concentration. Of the free sterols, stigmasterol showe...

journal_title：Plant physiology

authors： Grunwald C,Endress AG

更新日期：1985-01-01 00:00:00

abstract：:Genetic evidence in Arabidopsis (Arabidopsis thaliana) suggests that the auxin precursor indole-3-butyric acid (IBA) is converted into active indole-3-acetic acid (IAA) by peroxisomal beta-oxidation; however, direct evidence that Arabidopsis converts IBA to IAA is lacking, and the role of IBA-derived IAA is not well u...

journal_title：Plant physiology

authors： Strader LC,Culler AH,Cohen JD,Bartel B

更新日期：2010-08-01 00:00:00

abstract：:Abscisic acid (ABA), auxins, cytokinins, gibberellic acid, alone or in combination were tested for their effects on short-term sucrose uptake in sugar beet (Beta vulgaris cv USH-20) roots. The effect of ABA on active sucrose uptake varied from no effect to the more generally observed 1.4-to 3.0-fold stimulation. A rac...

journal_title：Plant physiology

authors： Saftner RA,Wyse RE

更新日期：1984-04-01 00:00:00

abstract：:The enzyme aldose (aldehyde) reductase was partially purified (142-fold) and characterized from Euonymus japonica leaves. The reductase, a dimer, had an average molecular weight of 67,000 as determined by gel filtration on Sephadex G-100. The enzyme was NADPH specific and reduced a broad range of substrates including ...

journal_title：Plant physiology

authors： Negm FB

更新日期：1986-04-01 00:00:00

abstract：:Soybean (Glycine max cv Hodgson) nitrogenase activity (C(2)H(2) reduction) in the presence or absence of nitrate was studied at various external O(2) tensions. Nitrogenase activity increased with oxygen partial pressure up to 30 kilopascals, which appeared to be the optimum. A parallel increase in ATP/ADP ratios indic...

journal_title：Plant physiology

authors： Heckmann MO,Drevon JJ,Saglio P,Salsac L

更新日期：1989-05-01 00:00:00

abstract：:The composition of the cell wall of the cotton fiber (Gossypium hirsutum L. Acala SJ-1) has been studied from the early stages of elongation (5 days postanthesis) through the period of secondary wall formation, using cell walls derived both from fibers developing on the plant and from fibers obtained from excised, cul...

journal_title：Plant physiology

authors： Meinert MC,Delmer DP

更新日期：1977-06-01 00:00:00

abstract：:We have used (13)C-labeled sugars and nuclear magnetic resonance (NMR) spectrometry to study the metabolic pathway of starch biosynthesis in developing wheat grain (Triticum aestivum cv Mardler). Our aim was to examine the extent of redistribution of (13)C between carbons atoms 1 and 6 of [1-(13)C] or [6-(13)C]glucose...

journal_title：Plant physiology

authors： Keeling PL,Wood JR,Tyson RH,Bridges IG

更新日期：1988-06-01 00:00:00

abstract：:The mechanisms and the controlling factors of malate oxidation by mitochondria from leaves of Kalanchoë blossfeldiana Poelln. plants performing Crassulacean acid metabolism were investigated using Percollpurified mitochondria. The effects of pH and of various cofactors (ATP, NAD(+), coenzyme A) on malate dehydrogenase...

journal_title：Plant physiology

authors： Rustin P,Lance C

更新日期：1986-08-01 00:00:00

abstract：:Low-molecular mass (10 kD) cytosolic acyl-coenzyme A-binding protein (ACBP) has a substantial influence over fatty acid (FA) composition in oilseeds, possibly via an effect on the partitioning of acyl groups between elongation and desaturation pathways. Previously, we demonstrated that the expression of a Brassica nap...

journal_title：Plant physiology

authors： Yurchenko O,Singer SD,Nykiforuk CL,Gidda S,Mullen RT,Moloney MM,Weselake RJ

更新日期：2014-06-01 00:00:00

abstract：:The uptake of 5-[(3)H]indol-3yl-acetic acid (IAA(*)) by segments of Zea mays L. roots was measured in the presence of nonradioactive indol-3yl-acetic acid (IAA degrees ) at different concentrations. IAA uptake was found to have a nonsaturable component and a saturable part with (at pH 5.0) an apparent K(m) of 0.285 mi...

journal_title：Plant physiology

authors： Martin HV,Pilet PE

更新日期：1986-07-01 00:00:00

abstract：:Boron (B) is required for cross linking of the pectic polysaccharide rhamnogalacturonan II (RG-II) and is consequently essential for the maintenance of cell wall structure. Arabidopsis (Arabidopsis thaliana) BOR1 is an efflux B transporter for xylem loading of B. Here, we describe the roles of BOR2, the most similar p...

journal_title：Plant physiology

authors： Miwa K,Wakuta S,Takada S,Ide K,Takano J,Naito S,Omori H,Matsunaga T,Fujiwara T

更新日期：2013-12-01 00:00:00

abstract：:The unicellular algae Chlorella sorokiniana was used as a model system to investigate the interaction of ozone with plant cell membranes. Ozone induces K(+) leakage from Chlorella sorokiniana similar to the electrolytic loss observed from many higher plants under stressful conditions. The kinetics of this leakage indi...

journal_title：Plant physiology

authors： Chimiklis PE,Heath RL

更新日期：1975-12-01 00:00:00

abstract：:Maize (Zea mays L.) endosperm suspension cultures are a useful model system for studying biochemical and physiological events in developing maize endosperm. In this report, sugar uptake by the cultures is characterized. Uptake of (14)C-labeled fructose and l-glucose was linear with time, while the rate of uptake of ra...

journal_title：Plant physiology

authors： Felker FC,Goodwin JC

更新日期：1988-12-01 00:00:00

abstract：:Levels of gibberillins (GAs) and of abscisic acid (ABA) in attached leaves of romaine lettuce (Lactuca sativa L.) declined as the leaf became older. The time course of changes in hormone levels, determined in detached lettuce leaves kept in darkness, revealed that a sharp decline in GAs accompanied by a moderate rise ...

journal_title：Plant physiology

authors： Aharoni N

更新日期：1978-08-01 00:00:00

abstract：:DNA and histone methylation coregulate heterochromatin formation and gene silencing in animals and plants. To identify factors involved in maintaining gene silencing, we conducted a forward genetic screen for mutants that release the silenced transgene Pro35S::NEOMYCIN PHOSPHOTRANSFERASE II in the transgenic Arabidops...

journal_title：Plant physiology

authors： Meng J,Wang L,Wang J,Zhao X,Cheng J,Yu W,Jin D,Li Q,Gong Z

更新日期：2018-06-01 00:00:00

abstract：:Zinc finger nucleases (ZFNs) are a powerful tool for genome editing in eukaryotic cells. ZFNs have been used for targeted mutagenesis in model and crop species. In animal and human cells, transient ZFN expression is often achieved by direct gene transfer into the target cells. Stable transformation, however, is the pr...

journal_title：Plant physiology

authors： Marton I,Zuker A,Shklarman E,Zeevi V,Tovkach A,Roffe S,Ovadis M,Tzfira T,Vainstein A

更新日期：2010-11-01 00:00:00

abstract：:A 2-step, 1-dimensional thin-layer chromatographic procedure for isolating indoleacetic acid (IAA) was developed and utilized in investigations of the biosynthesis of IAA from tryptophan-(14)C in cell-free extracts of pea (Pisum sativum L.) shoot tips. Identification of a (14)C-product as IAA was by (a) co-chromatogra...

journal_title：Plant physiology

authors： Moore TC,Shaner CA

更新日期：1967-12-01 00:00:00

abstract：:Glutamine synthetase (GS) from Synechocystis sp. PCC 6803 was inactivated in vivo by transferring cells from light to darkness or by incubation with the photosynthetic inhibitor 3-(3,4-dichlorophenyl)-1,1-dimethylurea but not with 2,5-dibromo-3-methyl-6-isopropyl-p-benzoquinone. Addition of glucose prevented both dark...

journal_title：Plant physiology

authors： Reyes JC,Crespo JL,Garcia-Dominguez M,Florencio FJ

更新日期：1995-11-01 00:00:00

abstract：:A recognition molecule was isolated from stigmas of S-allele genotype S(2)S(2) of Brassica oleracea var. capitata L. After Sephadex chromatography, it eluted as a single symmetrical peak during diethylaminoethane-cellulose chromatography. A high degree of purity was affirmed by: sedimentation as a single peak during u...

journal_title：Plant physiology

authors： Ferrari TE,Bruns D,Wallace DH

更新日期：1981-02-01 00:00:00

abstract：:Calmodulin antagonists, trifluoperazine, chlorpromazine, calmidazolium, N-(6-aminohexyl)-5-chloro-1-naphthalenesulfonamide (W-7), strongly inhibited the electrofusion of barley (Hordeum vulgare L. cv Moor) protoplasts with a marked increase of broken fusion products, after 60 minutes of incubation. W-5, a dechlorinate...

journal_title：Plant physiology

authors： Abe S,Takeda J

更新日期：1986-08-01 00:00:00

abstract：:Levels of ethylene biosynthesis and ethylene-inducible gene expression in wild-type tomato (Lycopersicon esculentum) fruit and in nonripening fruit from the tomato mutant rin (ripening inhibitor) were compared in order to investigate the mechanism of ethylene action. Whereas wild-type tomato fruit dramatically increas...

journal_title：Plant physiology

authors： Lincoln JE,Fischer RL

更新日期：1988-10-01 00:00:00

abstract：:Interrupting each long night with a prolonged period of far red radiant energy resulted in the inhibition of floral initiation in cocklebur. Irradiations inducing different relative levels of P(FR) from 1 to 2% to 80% had about the same effect under 4-hour photoperiods. The lower levels of P(FR) induced by continuous ...

journal_title：Plant physiology

authors： Mancinelli AL,Downs RJ

更新日期：1967-01-01 00:00:00

abstract：:Nearly all polysaccharides in plant cell walls are O-acetylated, including the various pectic polysaccharides and the hemicelluloses xylan, mannan, and xyloglucan. However, the enzymes involved in the polysaccharide acetylation have not been identified. While the role of polysaccharide acetylation in vivo is unclear, ...

journal_title：Plant physiology

authors： Manabe Y,Nafisi M,Verhertbruggen Y,Orfila C,Gille S,Rautengarten C,Cherk C,Marcus SE,Somerville S,Pauly M,Knox JP,Sakuragi Y,Scheller HV

更新日期：2011-03-01 00:00:00

abstract：:Changes in the galactose content of the noncellulosic polysaccharides of tomato (Mill) fruit cell walls were analyzed under various conditions. On the plant, galactan decreased gradually during fruit growth. As normal fruits ripened, the loss of galactan increased sharply; this was not observed in attached rin fruits ...

journal_title：Plant physiology

authors： Lackey GD,Gross KC,Wallner SJ

更新日期：1980-09-01 00:00:00

abstract：:RNA splicing is a fundamental mechanism contributing to the definition of the cellular protein population in any given environmental condition. DNA-DAMAGE REPAIR/TOLERATION PROTEIN111 (DRT111)/SPLICING FACTOR FOR PHYTOCHROME SIGNALING is a splicing factor previously shown to interact with phytochrome B and characteriz...

journal_title：Plant physiology

authors： Punzo P,Ruggiero A,Possenti M,Perrella G,Nurcato R,Costa A,Morelli G,Grillo S,Batelli G

更新日期：2020-06-01 00:00:00

abstract：:Intercellular distribution of enzymes involved in amino nitrogen synthesis was studied in leaves of species representing three C(4) groups, i.e. Sorghum bicolor, Zea mays, Digitaria sanguinalis (NADP malic enzyme type); Panicum miliaceum (NAD malic enzyme type); and Panicum maximum (phosphoenolpyruvate carboxykinase t...

journal_title：Plant physiology

authors： Rathnam CK,Edwards GE

更新日期：1976-06-01 00:00:00