Abstract:
:The uptake of 5-[(3)H]indol-3yl-acetic acid (IAA(*)) by segments of Zea mays L. roots was measured in the presence of nonradioactive indol-3yl-acetic acid (IAA degrees ) at different concentrations. IAA uptake was found to have a nonsaturable component and a saturable part with (at pH 5.0) an apparent K(m) of 0.285 micromolar and apparent V(max) 55.0 picomoles per gram fresh mass per minute. These results are consistent with those which might be expected for a saturable carrier capable of regulating IAA levels. High performance liquid chromatography analyses showed that very little metabolism of IAA(*) took place during 4 minute uptake experiments. Whereas nonsaturable uptake was similar for all 2 millimeter long segments prepared within the 2 to 10 millimeter region, saturable uptake was greatest for the 2 to 4 millimeter region. High levels of uptake by stelar (as compared with cortical) segments are partly attributable to the saturable carrier, and also to a high level of uptake by nonsaturable processes. The carrier may play an essential role in controlling IAA levels in maize roots, especially the accumulation of IAA in the apical region. The increase in saturable uptake toward the root tip may also contribute to the acropetal polarity of auxin transport.
journal_name
Plant Physioljournal_title
Plant physiologyauthors
Martin HV,Pilet PEdoi
10.1104/pp.81.3.889subject
Has Abstractpub_date
1986-07-01 00:00:00pages
889-95issue
3eissn
0032-0889issn
1532-2548journal_volume
81pub_type
杂志文章相关文献
PLANT PHYSIOLOGY文献大全abstract::The cytokinin activity has been isolated and identified from extracts of immature mango (Mangifera indica L.) seeds. The structures of zeatin, zeatin riboside, and N(6)-(Delta(2)-isopentenyl)adenine riboside were confirmed on the basis of their chromatographic behavior and mass spectra of trimethylsilyl derivatives. B...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.71.2.356
更新日期:1983-02-01 00:00:00
abstract::beta-Glucan synthetase activity in growing regions of pea (Pisum sativum L.) epicotyls was assayed by supplying UDP-glucose to particulate fractions of tissue homogenates or to thin tissue slices. Particulate fractions are less active in forming alkali-insoluble glucan than slices from the same tissue, although many k...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.61.6.938
更新日期:1978-06-01 00:00:00
abstract::Gibberellin A(1) (GA(1)), 3-epi-GA(1), GA(4), GA(9), 11alpha-hydroxyGA(12), 12alpha-hydroxyGA(12), GA(15), GA(17), GA(19), GA(20), GA(25), GA(37), GA(40), GA(58), GA(69), GA(70), and GA(71) have been identified from Kovats retention indices and full scan mass spectra by capillary GC-MS analyses of purified extracts fr...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.86.3.857
更新日期:1988-03-01 00:00:00
abstract::Rapidly dried Tortula ruralis, a drought-tolerant moss, is known to synthesize proteins on rehydration at a much lower rate than the slowly dried moss. The reasons for this low rate of protein synthesis are unclear. We have found that during rehydration of rapidly dried moss, there is a negative correlation between th...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.85.4.1094
更新日期:1987-12-01 00:00:00
abstract::Leaf tissues from Xerosicyos danguyi H. Humb., a succulent member of the Cucurbitaceae, were found to possess both galactinol synthase activity and the capacity for photosynthetic production of stachyose, the phloem transport oligosaccharide common to other nonsucculent cucurbits. The amounts of stachyose isolated fro...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.87.3.588
更新日期:1988-07-01 00:00:00
abstract::Isolated immature maize (Zea mays L.) embryos have been shown to acquire tolerance to rapid drying between 22 and 25 d after pollination (DAP) and to slow drying from 18 DAP onward. To investigate adaptations in protein profile in association with the acquisition of desiccation tolerance in isolated, immature maize em...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.116.3.1169
更新日期:1998-03-01 00:00:00
abstract::Young seedlings of Lavatera cretica L. exhibit positive phototropism. The hypocotyl perceives unilateral illumination with blue light and curves towards the light source by unequal growth. In addition, the cotyledonary laminas perceive the vectorial component of unilateral illumination with blue light and reorient nor...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.66.1.82
更新日期:1980-07-01 00:00:00
abstract::Plasmodesmata act as key elements in intercellular communication, coordinating processes related to plant growth, development, and responses to environmental stresses. While many of the developmental, biotic, and abiotic signals are primarily perceived at the plasma membrane (PM) by receptor proteins, plasmodesmata al...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.19.00473
更新日期:2019-09-01 00:00:00
abstract::Glyphosate, the most commonly used herbicide in the world, controls a wide range of plant species, mainly because plants have little capacity to metabolize (detoxify) glyphosate. Massive glyphosate use has led to world-wide evolution of glyphosate-resistant (GR) weed species, including the economically damaging grass ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.19.00979
更新日期:2019-12-01 00:00:00
abstract::In this communication we document the reproducible protocols for the purification of milligram quantities of cytochrome b(5) and NADH-cytochrome b(5) reductase from the microsomal fraction of Pisum sativum. The cytochrome b(5) component of this NADH linked electron transport chain was found to have a molecular mass of...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.85.2.457
更新日期:1987-10-01 00:00:00
abstract::Seeds of paper birch (Betula papyrifera Marsh.) were induced to germinate by prechilling at 3 C or by red light. The light requirement was mediated by phytochrome and the action of phytochrome during prechilling was investigated. Red irradiation (R) prior to prechilling markedly enhanced the effectiveness of the prech...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.67.4.705
更新日期:1981-04-01 00:00:00
abstract::The lv-1 mutant of pea (Pisum sativum L.) is deficient in responses regulated by phytochrome B (phyB) in other species but has normal levels of spectrally active phyB. We have characterized three further lv mutants (lv-2, lv-3, and lv-4), which are all elongated under red (R) and white light but are indistinguishable ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.108.2.525
更新日期:1995-06-01 00:00:00
abstract::The ripening of a fleshy fruit represents the summation of an array of biochemical processes that are regulated by interactions between developmental programs and environmental inputs. Analysis of tomato (Solanum lycopersicum) mutants and inhibitor studies indicate that ethylene is necessary for full development of th...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.104.057745
更新日期:2005-05-01 00:00:00
abstract::Fungal xylanases from Trichoderma spp. are potent elicitors of defense responses in various plants. To determine whether enzymatic activity is necessary for elicitor activity, we used site-directed mutagenesis to reduce the catalytic activity of xylanase II from Trichoderma reesei. For this, the glutamic acid residue ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.121.2.391
更新日期:1999-10-01 00:00:00
abstract::The responses of isoprene emission rate to temperature are characterized by complex time-dependent behaviors that are currently not entirely understood. To gain insight into the temperature dependencies of isoprene emission, we studied steady-state and transient responses of isoprene emission from hybrid aspen (Populu...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.110.162081
更新日期:2010-11-01 00:00:00
abstract::The biosynthetic pathway to n-heptane was investigated by examining the effect of the [beta]-keto acyl-acyl carrier protein synthase inhibitor (2R,3S)-2,3-epoxy-4-oxo-7E,10E-dodecadienamide (cerulenin), a thiol reagent ([beta]-mercaptoethanol), and an aldehydetrapping reagent (hydroxylamine) on the biosynthesis of n-[...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.111.4.1263
更新日期:1996-08-01 00:00:00
abstract::The addition of primary amines to the growth medium of the unicellular green alga Chlamydomonas reinhardtii disrupts cell wall assembly in both vegetative and zygotic cells. Primary amines are competitive inhibitors of the protein-cross-linking activity of transglutaminases. Two independent assays for transglutaminase...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.121.3.1003
更新日期:1999-11-01 00:00:00
abstract::Based on highly redundant and high-quality sequences, we assembled rice (Oryza sativa) mitochondrial genomes for two cultivars, 93-11 (an indica variety) and PA64S (an indica-like variety with maternal origin of japonica), which are paternal and maternal strains of an elite superhybrid rice Liang-You-Pei-Jiu (LYP-9), ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.105.070060
更新日期:2006-02-01 00:00:00
abstract::Tryptophan decarboxylase (TDC) is a cytosolic enzyme that catalyzes an early step of the terpenoid indole alkaloid biosynthetic pathway by decarboxylation of L-tryptophan to produce the protoalkaloid tryptamine. In the present study, recombinant TDC was targeted to the chloroplast, cytosol, and endoplasmic reticulum (...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.010889
更新日期:2002-07-01 00:00:00
abstract::The multifunctional movement protein (MP) of Tomato mosaic tobamovirus (ToMV) is involved in viral cell-to-cell movement, symptom development, and resistance gene recognition. However, it remains to be elucidated how ToMV MP plays such diverse roles in plants. Here, we show that ToMV MP interacts with the Rubisco smal...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.112.209213
更新日期:2013-01-01 00:00:00
abstract::Using tissue blotting and immunocytolocalization, we have investigated the appearance and accumulation of polygalacturonase (PG) during tomato (Lycopersicon esculentum Mill.) fruit ripening. Results show that PG first appears in the collumella region followed by sequential appearance in the exopericarp and endopericar...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.90.1.17
更新日期:1989-05-01 00:00:00
abstract::The activities of the free base and ribonucleoside forms of cytokinins bearing saturated and unsaturated N(6)-isoprenoid side chains have been examined in callus cultures derived from Phaseolus vulgaris cv. Great Northern, P. lunatus cv. Kingston, and the interspecific hybrid Great Northern x Kingston. In callus of cv...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.70.1.173
更新日期:1982-07-01 00:00:00
abstract::Apparent large size-classes of zein-synthesizing polysomes from developing kernels of Zea mays L. were converted to smaller polysomes after treatment with Protease K. The reduction in polysome size was not a result of ribonuclease activity, inasmuch as the enzyme did not affect the free polysomes or the size of the mR...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.60.4.482
更新日期:1977-10-01 00:00:00
abstract::[U-(14)C]glycine uptake into barley (Hordeum vulgare cv Hasso) vacuoles was investigated. Glycine (2 millimolar) transport was stimulated two- to fourfold by NaATP. Stimulation was saturable with respect to ATP (1 millimolar) and linear up to 20 millimolar glycine. Stimulation by NaATP was suppressed by Mg(2+) in equi...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.99.1.134
更新日期:1992-05-01 00:00:00
abstract::Increased ethylene synthesis was rapidly induced throughout the apical meristematic region of etiolated seedlings of Pisum sativum L., cv. Alaska by cuts made 1 centimeter from the apical hook. The wound signal was transmitted at about 2 millimeters per minute. Accumulation of substance(s) at the cut surfaces of excis...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.62.5.710
更新日期:1978-11-01 00:00:00
abstract::Glyoxysomes isolated from castor bean (Ricinus communis L. var. zanzibariensis) endosperm have been stained by the cytochemical diaminobenzidine reaction. The reaction product obtained by preincubation with 3,3'-diaminobenzidine and incubation with the reagent and H(2)O(2) is distributed uniformly throughout the matri...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.53.2.276
更新日期:1974-02-01 00:00:00
abstract::As a result of the phenylpropanoid pathway, many Brassicaceae produce considerable amounts of soluble hydroxycinnamate conjugates, mainly sinapate esters. From oilseed rape (Brassica napus), we cloned two orthologs of the Arabidopsis (Arabidopsis thaliana) gene reduced epidermal fluorescence1 (REF1) encoding a conifer...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.113.215491
更新日期:2013-04-01 00:00:00
abstract::Correlating measurements from differential scanning calorimetry, freeze-fracture freeze-etch electron microscopy, and survival of twigs after two-step cooling experiments, we provide strong evidence that winter-hardened Populus balsamifera v. virginiana (Sarg.) resists the stresses of freezing below -28 degrees C by a...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.79.1.41
更新日期:1985-09-01 00:00:00
abstract::The relationship between gravitropism and surface electrical potentials was studied using etiolated epicotyls of adzuki bean (Phaseolus angularis). Early downward curvature (or transient positive gravitropic response) was observed about 1 min after gravistimulation. The downward curvature was closely related to the sp...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.105.3.875
更新日期:1994-07-01 00:00:00
abstract::Cadmium complexes with apparent molecular weights of 10,000 were observed in aqueous extracts of Cd-treated cabbage (Brassica capitata L., cv. red danish) and tobacco (hybrid of Nicotiana glauca and N. langsdorffii) plants. The amount of complex (as Cd) recovered was found to be dependent on the concentration of the m...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.69.4.804
更新日期:1982-04-01 00:00:00