Saturable uptake of indol-3yl-acetic Acid by maize roots.

Abstract:

:The uptake of 5-[(3)H]indol-3yl-acetic acid (IAA(*)) by segments of Zea mays L. roots was measured in the presence of nonradioactive indol-3yl-acetic acid (IAA degrees ) at different concentrations. IAA uptake was found to have a nonsaturable component and a saturable part with (at pH 5.0) an apparent K(m) of 0.285 micromolar and apparent V(max) 55.0 picomoles per gram fresh mass per minute. These results are consistent with those which might be expected for a saturable carrier capable of regulating IAA levels. High performance liquid chromatography analyses showed that very little metabolism of IAA(*) took place during 4 minute uptake experiments. Whereas nonsaturable uptake was similar for all 2 millimeter long segments prepared within the 2 to 10 millimeter region, saturable uptake was greatest for the 2 to 4 millimeter region. High levels of uptake by stelar (as compared with cortical) segments are partly attributable to the saturable carrier, and also to a high level of uptake by nonsaturable processes. The carrier may play an essential role in controlling IAA levels in maize roots, especially the accumulation of IAA in the apical region. The increase in saturable uptake toward the root tip may also contribute to the acropetal polarity of auxin transport.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Martin HV,Pilet PE

doi

10.1104/pp.81.3.889

subject

Has Abstract

pub_date

1986-07-01 00:00:00

pages

889-95

issue

3

eissn

0032-0889

issn

1532-2548

journal_volume

81

pub_type

杂志文章
  • Cytokinins of the Developing Mango Fruit : Isolation, Identification, and Changes in Levels during Maturation.

    abstract::The cytokinin activity has been isolated and identified from extracts of immature mango (Mangifera indica L.) seeds. The structures of zeatin, zeatin riboside, and N(6)-(Delta(2)-isopentenyl)adenine riboside were confirmed on the basis of their chromatographic behavior and mass spectra of trimethylsilyl derivatives. B...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.71.2.356

    authors: Chen WS

    更新日期:1983-02-01 00:00:00

  • Tissue Slice and Particulate beta-Glucan Synthetase Activities from Pisum Epicotyls.

    abstract::beta-Glucan synthetase activity in growing regions of pea (Pisum sativum L.) epicotyls was assayed by supplying UDP-glucose to particulate fractions of tissue homogenates or to thin tissue slices. Particulate fractions are less active in forming alkali-insoluble glucan than slices from the same tissue, although many k...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.61.6.938

    authors: Raymond Y,Fincher GB,Maclachlan GA

    更新日期:1978-06-01 00:00:00

  • Endogenous Gibberellins from Sporophytes of Two Tree Ferns, Cibotium glaucum and Dicksonia antarctica.

    abstract::Gibberellin A(1) (GA(1)), 3-epi-GA(1), GA(4), GA(9), 11alpha-hydroxyGA(12), 12alpha-hydroxyGA(12), GA(15), GA(17), GA(19), GA(20), GA(25), GA(37), GA(40), GA(58), GA(69), GA(70), and GA(71) have been identified from Kovats retention indices and full scan mass spectra by capillary GC-MS analyses of purified extracts fr...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.86.3.857

    authors: Yamane H,Fujioka S,Spray CR,Phinney BO,Macmillan J,Gaskin P,Takahashi N

    更新日期:1988-03-01 00:00:00

  • Protein Synthesis during Rehydration of Rapidly Dried Tortula ruralis: Evidence for Oxidation Injury.

    abstract::Rapidly dried Tortula ruralis, a drought-tolerant moss, is known to synthesize proteins on rehydration at a much lower rate than the slowly dried moss. The reasons for this low rate of protein synthesis are unclear. We have found that during rehydration of rapidly dried moss, there is a negative correlation between th...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.85.4.1094

    authors: Dhindsa RS

    更新日期:1987-12-01 00:00:00

  • Stachyose Synthesis in Source Leaf Tissues of the CAM Plant Xerosicyos danguyi H. Humb.

    abstract::Leaf tissues from Xerosicyos danguyi H. Humb., a succulent member of the Cucurbitaceae, were found to possess both galactinol synthase activity and the capacity for photosynthetic production of stachyose, the phloem transport oligosaccharide common to other nonsucculent cucurbits. The amounts of stachyose isolated fro...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.87.3.588

    authors: Madore MA,Mitchell DE,Boyd CM

    更新日期:1988-07-01 00:00:00

  • Fourier transform infrared microspectroscopy detects changes in protein secondary structure associated with desiccation tolerance in developing maize embryos

    abstract::Isolated immature maize (Zea mays L.) embryos have been shown to acquire tolerance to rapid drying between 22 and 25 d after pollination (DAP) and to slow drying from 18 DAP onward. To investigate adaptations in protein profile in association with the acquisition of desiccation tolerance in isolated, immature maize em...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.116.3.1169

    authors: Wolkers WF,Bochicchio A,Selvaggi G,Hoekstra FA

    更新日期:1998-03-01 00:00:00

  • Role of the Cotyledons in the Phototropic Response of Lavatera cretica Seedlings.

    abstract::Young seedlings of Lavatera cretica L. exhibit positive phototropism. The hypocotyl perceives unilateral illumination with blue light and curves towards the light source by unequal growth. In addition, the cotyledonary laminas perceive the vectorial component of unilateral illumination with blue light and reorient nor...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.66.1.82

    authors: Schwartz A,Koller D

    更新日期:1980-07-01 00:00:00

  • Plasma Membrane-Associated Receptor-like Kinases Relocalize to Plasmodesmata in Response to Osmotic Stress.

    abstract::Plasmodesmata act as key elements in intercellular communication, coordinating processes related to plant growth, development, and responses to environmental stresses. While many of the developmental, biotic, and abiotic signals are primarily perceived at the plasma membrane (PM) by receptor proteins, plasmodesmata al...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.19.00473

    authors: Grison MS,Kirk P,Brault ML,Wu XN,Schulze WX,Benitez-Alfonso Y,Immel F,Bayer EM

    更新日期:2019-09-01 00:00:00

  • Aldo-keto Reductase Metabolizes Glyphosate and Confers Glyphosate Resistance in Echinochloa colona.

    abstract::Glyphosate, the most commonly used herbicide in the world, controls a wide range of plant species, mainly because plants have little capacity to metabolize (detoxify) glyphosate. Massive glyphosate use has led to world-wide evolution of glyphosate-resistant (GR) weed species, including the economically damaging grass ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.19.00979

    authors: Pan L,Yu Q,Han H,Mao L,Nyporko A,Fan L,Bai L,Powles S

    更新日期:2019-12-01 00:00:00

  • Purification and Characterization of Microsomal Cytochrome b(5) and NADH Cytochrome b(5) Reductase from Pisum sativum.

    abstract::In this communication we document the reproducible protocols for the purification of milligram quantities of cytochrome b(5) and NADH-cytochrome b(5) reductase from the microsomal fraction of Pisum sativum. The cytochrome b(5) component of this NADH linked electron transport chain was found to have a molecular mass of...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.85.2.457

    authors: Jollie DR,Sligar SG,Schuler M

    更新日期:1987-10-01 00:00:00

  • Phytochrome Action during Prechilling Induced Germination of Betula papyrifera Marsh.

    abstract::Seeds of paper birch (Betula papyrifera Marsh.) were induced to germinate by prechilling at 3 C or by red light. The light requirement was mediated by phytochrome and the action of phytochrome during prechilling was investigated. Red irradiation (R) prior to prechilling markedly enhanced the effectiveness of the prech...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.67.4.705

    authors: Bevington JM

    更新日期:1981-04-01 00:00:00

  • New lv Mutants of Pea Are Deficient in Phytochrome B.

    abstract::The lv-1 mutant of pea (Pisum sativum L.) is deficient in responses regulated by phytochrome B (phyB) in other species but has normal levels of spectrally active phyB. We have characterized three further lv mutants (lv-2, lv-3, and lv-4), which are all elongated under red (R) and white light but are indistinguishable ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.108.2.525

    authors: Weller JL,Nagatani A,Kendrick RE,Murfet IC,Reid JB

    更新日期:1995-06-01 00:00:00

  • Ethylene insensitivity conferred by the Green-ripe and Never-ripe 2 ripening mutants of tomato.

    abstract::The ripening of a fleshy fruit represents the summation of an array of biochemical processes that are regulated by interactions between developmental programs and environmental inputs. Analysis of tomato (Solanum lycopersicum) mutants and inhibitor studies indicate that ethylene is necessary for full development of th...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.104.057745

    authors: Barry CS,McQuinn RP,Thompson AJ,Seymour GB,Grierson D,Giovannoni JJ

    更新日期:2005-05-01 00:00:00

  • The enzymatic activity of fungal xylanase is not necessary for its elicitor activity.

    abstract::Fungal xylanases from Trichoderma spp. are potent elicitors of defense responses in various plants. To determine whether enzymatic activity is necessary for elicitor activity, we used site-directed mutagenesis to reduce the catalytic activity of xylanase II from Trichoderma reesei. For this, the glutamic acid residue ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.121.2.391

    authors: Enkerli J,Felix G,Boller T

    更新日期:1999-10-01 00:00:00

  • Temperature response of isoprene emission in vivo reflects a combined effect of substrate limitations and isoprene synthase activity: a kinetic analysis.

    abstract::The responses of isoprene emission rate to temperature are characterized by complex time-dependent behaviors that are currently not entirely understood. To gain insight into the temperature dependencies of isoprene emission, we studied steady-state and transient responses of isoprene emission from hybrid aspen (Populu...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.110.162081

    authors: Rasulov B,Hüve K,Bichele I,Laisk A,Niinemets U

    更新日期:2010-11-01 00:00:00

  • Evidence for an Elongation/Reduction/C1-Elimination Pathway in the Biosynthesis of n-Heptane in Xylem of Jeffrey Pine.

    abstract::The biosynthetic pathway to n-heptane was investigated by examining the effect of the [beta]-keto acyl-acyl carrier protein synthase inhibitor (2R,3S)-2,3-epoxy-4-oxo-7E,10E-dodecadienamide (cerulenin), a thiol reagent ([beta]-mercaptoethanol), and an aldehydetrapping reagent (hydroxylamine) on the biosynthesis of n-[...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.111.4.1263

    authors: Savage TJ,Hristova MK,Croteau R

    更新日期:1996-08-01 00:00:00

  • A transglutaminase immunologically related to tissue transglutaminase catalyzes cross-linking of cell wall proteins in Chlamydomonas reinhardtii.

    abstract::The addition of primary amines to the growth medium of the unicellular green alga Chlamydomonas reinhardtii disrupts cell wall assembly in both vegetative and zygotic cells. Primary amines are competitive inhibitors of the protein-cross-linking activity of transglutaminases. Two independent assays for transglutaminase...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.121.3.1003

    authors: Waffenschmidt S,Kusch T,Woessner JP

    更新日期:1999-11-01 00:00:00

  • The rice mitochondrial genomes and their variations.

    abstract::Based on highly redundant and high-quality sequences, we assembled rice (Oryza sativa) mitochondrial genomes for two cultivars, 93-11 (an indica variety) and PA64S (an indica-like variety with maternal origin of japonica), which are paternal and maternal strains of an elite superhybrid rice Liang-You-Pei-Jiu (LYP-9), ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.105.070060

    authors: Tian X,Zheng J,Hu S,Yu J

    更新日期:2006-02-01 00:00:00

  • Targeting tryptophan decarboxylase to selected subcellular compartments of tobacco plants affects enzyme stability and in vivo function and leads to a lesion-mimic phenotype.

    abstract::Tryptophan decarboxylase (TDC) is a cytosolic enzyme that catalyzes an early step of the terpenoid indole alkaloid biosynthetic pathway by decarboxylation of L-tryptophan to produce the protoalkaloid tryptamine. In the present study, recombinant TDC was targeted to the chloroplast, cytosol, and endoplasmic reticulum (...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.010889

    authors: Di Fiore S,Li Q,Leech MJ,Schuster F,Emans N,Fischer R,Schillberg S

    更新日期:2002-07-01 00:00:00

  • The rubisco small subunit is involved in tobamovirus movement and Tm-2²-mediated extreme resistance.

    abstract::The multifunctional movement protein (MP) of Tomato mosaic tobamovirus (ToMV) is involved in viral cell-to-cell movement, symptom development, and resistance gene recognition. However, it remains to be elucidated how ToMV MP plays such diverse roles in plants. Here, we show that ToMV MP interacts with the Rubisco smal...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.112.209213

    authors: Zhao J,Liu Q,Zhang H,Jia Q,Hong Y,Liu Y

    更新日期:2013-01-01 00:00:00

  • Immunocytolocalization of polygalacturonase in ripening tomato fruit.

    abstract::Using tissue blotting and immunocytolocalization, we have investigated the appearance and accumulation of polygalacturonase (PG) during tomato (Lycopersicon esculentum Mill.) fruit ripening. Results show that PG first appears in the collumella region followed by sequential appearance in the exopericarp and endopericar...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.90.1.17

    authors: Tieman DM,Handa AK

    更新日期:1989-05-01 00:00:00

  • Cytokinin structure-activity relationships and the metabolism of N-(delta-isopentenyl)adenosine-8-C in phaseolus callus tissues.

    abstract::The activities of the free base and ribonucleoside forms of cytokinins bearing saturated and unsaturated N(6)-isoprenoid side chains have been examined in callus cultures derived from Phaseolus vulgaris cv. Great Northern, P. lunatus cv. Kingston, and the interspecific hybrid Great Northern x Kingston. In callus of cv...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.70.1.173

    authors: Mok MC,Mok DW,Dixon SC,Armstrong DJ,Shaw G

    更新日期:1982-07-01 00:00:00

  • Dissociation of polysome aggregates by protease k.

    abstract::Apparent large size-classes of zein-synthesizing polysomes from developing kernels of Zea mays L. were converted to smaller polysomes after treatment with Protease K. The reduction in polysome size was not a result of ribonuclease activity, inasmuch as the enzyme did not affect the free polysomes or the size of the mR...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.60.4.482

    authors: Larkins BA,Tsai CY

    更新日期:1977-10-01 00:00:00

  • Glycine uptake into barley mesophyll vacuoles is regulated but not energized by ATP.

    abstract::[U-(14)C]glycine uptake into barley (Hordeum vulgare cv Hasso) vacuoles was investigated. Glycine (2 millimolar) transport was stimulated two- to fourfold by NaATP. Stimulation was saturable with respect to ATP (1 millimolar) and linear up to 20 millimolar glycine. Stimulation by NaATP was suppressed by Mg(2+) in equi...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.99.1.134

    authors: Goerlach J,Willms-Hoff I

    更新日期:1992-05-01 00:00:00

  • Rapidly Induced Wound Ethylene from Excised Segments of Etiolated Pisum sativum L., cv. Alaska: III. Induction and Transmission of the Response.

    abstract::Increased ethylene synthesis was rapidly induced throughout the apical meristematic region of etiolated seedlings of Pisum sativum L., cv. Alaska by cuts made 1 centimeter from the apical hook. The wound signal was transmitted at about 2 millimeters per minute. Accumulation of substance(s) at the cut surfaces of excis...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.62.5.710

    authors: Saltveit ME,Dilley DR

    更新日期:1978-11-01 00:00:00

  • Cytochemical localization of catalase activity in glyoxysomes from castor bean endosperm.

    abstract::Glyoxysomes isolated from castor bean (Ricinus communis L. var. zanzibariensis) endosperm have been stained by the cytochemical diaminobenzidine reaction. The reaction product obtained by preincubation with 3,3'-diaminobenzidine and incubation with the reagent and H(2)O(2) is distributed uniformly throughout the matri...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.53.2.276

    authors: Bieglmayer C,Ruis H

    更新日期:1974-02-01 00:00:00

  • Reprogramming the phenylpropanoid metabolism in seeds of oilseed rape by suppressing the orthologs of reduced epidermal fluorescence1.

    abstract::As a result of the phenylpropanoid pathway, many Brassicaceae produce considerable amounts of soluble hydroxycinnamate conjugates, mainly sinapate esters. From oilseed rape (Brassica napus), we cloned two orthologs of the Arabidopsis (Arabidopsis thaliana) gene reduced epidermal fluorescence1 (REF1) encoding a conifer...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.113.215491

    authors: Mittasch J,Böttcher C,Frolov A,Strack D,Milkowski C

    更新日期:2013-04-01 00:00:00

  • A novel method of natural cryoprotection : intracellular glass formation in deeply frozen populus.

    abstract::Correlating measurements from differential scanning calorimetry, freeze-fracture freeze-etch electron microscopy, and survival of twigs after two-step cooling experiments, we provide strong evidence that winter-hardened Populus balsamifera v. virginiana (Sarg.) resists the stresses of freezing below -28 degrees C by a...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.79.1.41

    authors: Hirsh AG,Williams RJ,Meryman HT

    更新日期:1985-09-01 00:00:00

  • Early Gravi-Electrical Responses in Bean Epicotyls.

    abstract::The relationship between gravitropism and surface electrical potentials was studied using etiolated epicotyls of adzuki bean (Phaseolus angularis). Early downward curvature (or transient positive gravitropic response) was observed about 1 min after gravistimulation. The downward curvature was closely related to the sp...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.105.3.875

    authors: Shigematsu H,Toko K,Matsuno T,Yamafuji K

    更新日期:1994-07-01 00:00:00

  • Inducible cadmium binding complexes of cabbage and tobacco.

    abstract::Cadmium complexes with apparent molecular weights of 10,000 were observed in aqueous extracts of Cd-treated cabbage (Brassica capitata L., cv. red danish) and tobacco (hybrid of Nicotiana glauca and N. langsdorffii) plants. The amount of complex (as Cd) recovered was found to be dependent on the concentration of the m...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.69.4.804

    authors: Wagner GJ,Trotter MM

    更新日期:1982-04-01 00:00:00