DRT111/SFPS Splicing Factor Controls Abscisic Acid Sensitivity during Seed Development and Germination.

Abstract:

:RNA splicing is a fundamental mechanism contributing to the definition of the cellular protein population in any given environmental condition. DNA-DAMAGE REPAIR/TOLERATION PROTEIN111 (DRT111)/SPLICING FACTOR FOR PHYTOCHROME SIGNALING is a splicing factor previously shown to interact with phytochrome B and characterized for its role in splicing of pre-mRNAs involved in photomorphogenesis. Here, we show that DRT111 interacts with Arabidopsis (Arabidopsis thaliana) Splicing Factor1, involved in 3' splicing site recognition. Double- and triple-mutant analysis shows that DRT111 controls splicing of ABI3 and acts upstream of the splicing factor SUPPRESSOR OF ABI3-ABI5. DRT111 is highly expressed in seeds and stomata of Arabidopsis and is induced by long-term treatments of polyethylene glycol and abscisic acid (ABA). DRT111 knock-out mutants are defective in ABA-induced stomatal closure and are hypersensitive to ABA during seed germination. Conversely, DRT111 overexpressing plants show ABA-hyposensitive seed germination. RNA-sequencing experiments show that in dry seeds, DRT111 controls expression and splicing of genes involved in osmotic-stress and ABA responses, light signaling, and mRNA splicing, including targets of ABSCISIC ACID INSENSITIVE3 (ABI3) and PHYTOCHROME INTERACTING FACTORs (PIFs). Consistently, expression of the germination inhibitor SOMNUS, induced by ABI3 and PIF1, is upregulated in imbibed seeds of drt111-2 mutants. Together, these results indicate that DRT111 controls sensitivity to ABA during seed development, germination, and stomatal movements, and integrates ABA- and light-regulated pathways to control seed germination.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Punzo P,Ruggiero A,Possenti M,Perrella G,Nurcato R,Costa A,Morelli G,Grillo S,Batelli G

doi

10.1104/pp.20.00037

subject

Has Abstract

pub_date

2020-06-01 00:00:00

pages

793-807

issue

2

eissn

0032-0889

issn

1532-2548

pii

pp.20.00037

journal_volume

183

pub_type

杂志文章
  • Arabidopsis INOSITOL TRANSPORTER2 mediates H+ symport of different inositol epimers and derivatives across the plasma membrane.

    abstract::Of the four genes of the Arabidopsis (Arabidopsis thaliana) INOSITOL TRANSPORTER family (AtINT family) so far only AtINT4 has been described. Here we present the characterization of AtINT2 and AtINT3. cDNA sequencing revealed that the AtINT3 gene is incorrectly spliced and encodes a truncated protein of only 182 amino...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.107.109033

    authors: Schneider S,Schneidereit A,Udvardi P,Hammes U,Gramann M,Dietrich P,Sauer N

    更新日期:2007-12-01 00:00:00

  • Specificity of cycloheximide in higher plant systems.

    abstract::Although cycloheximide is extremely inhibitory to protein synthesis in vivo in higher plants, the reported insensitivity of some plant ribosomes suggests that it may not invariably act at the ribosomal level. This suggestion is reinforced by results obtained with red beet storage tissue disks, the respiration of which...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.46.2.227

    authors: Ellis RJ,Macdonald IR

    更新日期:1970-08-01 00:00:00

  • Purification and Characterization of Extracellular Pectinesterases from Phytophthora infestans.

    abstract::Constitutively produced extracellular pectinesterases from culture filtrates of the potato late blight fungus Phytophthora infestans were purified and characterized. One enzyme (PE II) was purified to homogeneity. Sodium dodecyl sulfate electrophoresis of the second enzyme (PE I) revealed two protein bands; there are ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.77.1.109

    authors: Förster H,Rasched I

    更新日期:1985-01-01 00:00:00

  • Light and CO(2) Response of Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase Activation in Arabidopsis Leaves.

    abstract::The requirements for activation of ribulose 1,5-bisphosphate carboxylase/oxygenase (rubisco) were investigated in leaves of Arabidopsis wild-type and a mutant incapable of light activating rubisco in vivo. Upon illumination with saturating light intensities, the activation state of rubisco increased 2-fold in the wild...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.80.3.655

    authors: Salvucci ME,Portis AR,Ogren WL

    更新日期:1986-03-01 00:00:00

  • Role of the Cotyledons in the Phototropic Response of Lavatera cretica Seedlings.

    abstract::Young seedlings of Lavatera cretica L. exhibit positive phototropism. The hypocotyl perceives unilateral illumination with blue light and curves towards the light source by unequal growth. In addition, the cotyledonary laminas perceive the vectorial component of unilateral illumination with blue light and reorient nor...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.66.1.82

    authors: Schwartz A,Koller D

    更新日期:1980-07-01 00:00:00

  • Voltage-sensor transitions of the inward-rectifying K+ channel KAT1 indicate a latching mechanism biased by hydration within the voltage sensor.

    abstract::The Kv-like (potassium voltage-dependent) K(+) channels at the plasma membrane, including the inward-rectifying KAT1 K(+) channel of Arabidopsis (Arabidopsis thaliana), are important targets for manipulating K(+) homeostasis in plants. Gating modification, especially, has been identified as a promising means by which ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.114.244319

    authors: Lefoulon C,Karnik R,Honsbein A,Gutla PV,Grefen C,Riedelsberger J,Poblete T,Dreyer I,Gonzalez W,Blatt MR

    更新日期:2014-10-01 00:00:00

  • Sequence and comparative analysis of the maize NB mitochondrial genome.

    abstract::The NB mitochondrial genome found in most fertile varieties of commercial maize (Zea mays subsp. mays) was sequenced. The 569,630-bp genome maps as a circle containing 58 identified genes encoding 33 known proteins, 3 ribosomal RNAs, and 21 tRNAs that recognize 14 amino acids. Among the 22 group II introns identified,...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.104.044602

    authors: Clifton SW,Minx P,Fauron CM,Gibson M,Allen JO,Sun H,Thompson M,Barbazuk WB,Kanuganti S,Tayloe C,Meyer L,Wilson RK,Newton KJ

    更新日期:2004-11-01 00:00:00

  • Nitric oxide mediates the fungal elicitor-induced hypericin production of Hypericum perforatum cell suspension cultures through a jasmonic-acid-dependent signal pathway.

    abstract::Fungal elicitor prepared from the cell walls of Aspergillum niger induces multiple responses of Hypericum perforatum cells, including nitric oxide (NO) generation, jasmonic acid (JA) biosynthesis, and hypericin production. To determine the role of NO and JA in elicitor-induced hypericin production, we study the effect...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.105.066407

    authors: Xu MJ,Dong JF,Zhu MY

    更新日期:2005-10-01 00:00:00

  • Molecular identification of an Arabidopsis S-adenosylmethionine transporter. Analysis of organ distribution, bacterial expression, reconstitution into liposomes, and functional characterization.

    abstract::Despite much study of the role of S-adenosylmethionine (SAM) in the methylation of DNA, RNA, and proteins, and as a cofactor for a wide range of biosynthetic processes, little is known concerning the intracellular transport of this essential metabolite. Screening of the Arabidopsis (Arabidopsis thaliana) genome yielde...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.106.086975

    authors: Palmieri L,Arrigoni R,Blanco E,Carrari F,Zanor MI,Studart-Guimaraes C,Fernie AR,Palmieri F

    更新日期:2006-11-01 00:00:00

  • Expression of DNA coding for diphtheria toxin chain a is toxic to plant cells.

    abstract::DNA coding for the enzymatically active subunit A of diphtheria toxin was placed under the control of the cauliflower mosaic virus 35S promoter and the Agrobacterium left transfer-DNA gene 7 polyadenylation signal. Agrobacteria carrying a binary plant vector with the chimeric diphtheria toxin A gene had very low trans...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.95.3.687

    authors: Czako M,An G

    更新日期:1991-03-01 00:00:00

  • The interplay of light and the circadian clock. Independent dual regulation of clock-controlled gene ccg-2(eas).

    abstract::Ambient light is the major agent mediating entrainment of circadian rhythms and is also a major factor influencing development and morphogenesis. We show that in Neurospora crassa the expression of clock-controlled gene 2 (ccg-2), a gene under the control of the circadian clock and allelic to the developmental gene ea...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.102.4.1299

    authors: Arpaia G,Loros JJ,Dunlap JC,Morelli G,Macino G

    更新日期:1993-08-01 00:00:00

  • Gravitropic response of inflorescence stems in Arabidopsis thaliana.

    abstract::We have characterized the gravitropic response of inflorescence stems in Arabidopsis thaliana. When the inflorescence stems were placed horizontally, they curved upward about 90 degrees within 90 min in darkness at 23 degrees C, exhibiting strong negative gravitropism. Decapitated stem segments (without all flowers, f...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.110.3.933

    authors: Fukaki H,Fujisawa H,Tasaka M

    更新日期:1996-03-01 00:00:00

  • Storage Compartments for Capillary Water Rarely Refill in an Intact Woody Plant.

    abstract::Water storage is thought to play an integral role in the maintenance of whole-plant water balance. The contribution of both living and dead cells to water storage can be derived from rehydration and water-release curves on excised plant material, but the underlying tissue-specific emptying/refilling dynamics remain un...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.17.01133

    authors: Knipfer T,Cuneo IF,Earles JM,Reyes C,Brodersen CR,McElrone AJ

    更新日期:2017-12-01 00:00:00

  • A Photorespiratory Mutant of Chlamydomonas reinhardtii.

    abstract::A mutant strain of Chlamydomonas reinhardtii, designated 18-7F, has been isolated and characterized. 18-7F requires a high CO(2) concentration for photoautrophic growth in spite of the apparent induction of a functional CO(2) concentrating mechanism in air-adapted cells. In 2% O(2) the photosynthetic characteristics o...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.93.1.231

    authors: Suzuki K,Marek LF,Spalding MH

    更新日期:1990-05-01 00:00:00

  • Monoterpene metabolism. Cloning, expression, and characterization of (-)-isopiperitenol/(-)-carveol dehydrogenase of peppermint and spearmint.

    abstract::The essential oils of peppermint (Mentha x piperita) and spearmint (Mentha spicata) are distinguished by the oxygenation position on the p-menthane ring of the constitutive monoterpenes that is conferred by two regiospecific cytochrome P450 limonene-3- and limonene-6-hydroxylases. Following hydroxylation of limonene, ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.104.053298

    authors: Ringer KL,Davis EM,Croteau R

    更新日期:2005-03-01 00:00:00

  • Iron uptake by plants from microbial siderophores : a study with 7-nitrobenz-2 oxa-1,3-diazole-desferrioxamine as fluorescent ferrioxamine B analog.

    abstract::The synthetically produced fluorescent siderophore NBD-desferrioxamine B (NBD-DFO), an analog of the natural siderophore ferrioxamine B, was used to study iron uptake by plants. Short-term (10-hour) (55)Fe uptake rates by cotton (Gossypium spp.) and maize (Zea mays L.) plants from the modified siderophore were similar...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.99.4.1329

    authors: Bar-Ness E,Hadar Y,Chen Y,Shanzer A,Libman J

    更新日期:1992-08-01 00:00:00

  • Inhibition of the formation of photosynthetic enzymes by inhibitors of photosynthesis.

    abstract::It has been shown previously that an increase in ribulose diphosphate carboxylase activity occurs upon brief illumination of leaves of dark-grown Zea mays plants; an increase in ribose 5-phosphate isomerase occurs after prolonged illumination. Both of these responses to illumination are inhibited by chloramphenicol.Th...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.43.2.188

    authors: McMahon D,Bogorad L

    更新日期:1968-02-01 00:00:00

  • Dissociation of polysome aggregates by protease k.

    abstract::Apparent large size-classes of zein-synthesizing polysomes from developing kernels of Zea mays L. were converted to smaller polysomes after treatment with Protease K. The reduction in polysome size was not a result of ribonuclease activity, inasmuch as the enzyme did not affect the free polysomes or the size of the mR...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.60.4.482

    authors: Larkins BA,Tsai CY

    更新日期:1977-10-01 00:00:00

  • An early response to gibberellic Acid not requiring protein synthesis.

    abstract::Cell-free extracts from gibberellic acid-treated barley (Hordeum vulgare L. cv. Himalaya) aleurone layers show phosphorylcholine glyceride transferase activity greater than that from control layers. The increase in activity is not prevented by a mixture of amino acid analogs nor by cordycepin under conditions in which...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.54.6.813

    authors: Ben-Tal Y

    更新日期:1974-12-01 00:00:00

  • The PsENOD12 Gene Is Expressed at Two Different Sites in Afghanistan Pea Pseudonodules Induced by Auxin Transport Inhibitors.

    abstract::A number of early nodulin genes are expressed in specific cell types as pea (Pisum sativum) root nodules develop. The Pisum sativum early nodulin PsENOD2 is detected only in the uninfected cells of the nodule parenchyma, whereas PsENOD12 is expressed at two spatially removed sites: in root hairs and adjacent cortical ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.100.4.1649

    authors: Scheres B,McKhann HI,Zalensky A,Löbler M,Bisseling T,Hirsch AM

    更新日期:1992-12-01 00:00:00

  • Enzymic Mechanism of Starch Breakdown in Germinating Rice Seeds: 8. Immunohistochemical Localization of beta-Amylase.

    abstract::Rabbit antiserum against beta-amylase isolated from germinating seeds of rice was produced, and its specific cross-reactivity with beta-amylase was confirmed by means of Ouchterlony double immunodiffusion and immunoelectrophoresis procedures. The cellular localization of beta-amylase was studied by indirect fluorescen...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.64.2.337

    authors: Okamoto K,Akazawa T

    更新日期:1979-08-01 00:00:00

  • CYP79D73 Participates in Biosynthesis of Floral Scent Compound 2-Phenylethanol in Plumeria rubra.

    abstract::Plumeria (Plumeria rubra), well known for its brightly colored and fragrant flowers, emits a number of floral volatile organic compounds (VOCs). Plumeria flowers emit a total of 43 VOCs including nine phenylpropanoids/benzenoids, such as 2-phenylethanol (2PE), benzaldehyde, 2-phenylacetaldehyde (PAld), (E/Z)-phenylace...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.19.00098

    authors: Dhandapani S,Jin J,Sridhar V,Chua NH,Jang IC

    更新日期:2019-05-01 00:00:00

  • Physiological Control of Chloride Transport in Chara corallina: I. EFFECTS OF LOW TEMPERATURE, CELL TURGOR PRESSURE, AND ANIONS.

    abstract::The rate of Cl(-) transport at the plasma membrane of the freshwater alga Chara corallina is investigated with respect to possible in vivo controls acting in addition to the two well established ones of cytoplasmic Cl(-) and cytoplasmic pH. In contrast with results from many other plant tissues, halides appear to be t...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.67.6.1113

    authors: Sanders D

    更新日期:1981-06-01 00:00:00

  • Interaction of water supply and N in wheat.

    abstract::The purpose of this study was to investigate effects of N nutrition and water stress on stomatal behavior and CO(2) exchange rate in wheat (Triticum aestivum L. cv Olaf). Wheat plants were grown hydroponically with high (100 milligrams per liter) and low (10 milligrams per liter) N. When plants were 38 days old, a 24-...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.76.1.112

    authors: Morgan JA

    更新日期:1984-09-01 00:00:00

  • Induction and Accumulation of Heat Shock-Specific Poly(A) RNAs and Proteins in Soybean Seedlings during Arsenite and Cadmium Treatments.

    abstract::Northern blot hybridization analyzes revealed that poly(A(+)) RNAs homologous to eight heat shock (HS)-specific cDNA clones were induced by arsenite (As) or Cd treatments. The mRNAs accumulated slower, and maximum accumulations were consistently lower than HS-induced levels. Prolonged treatment with low concentrations...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.86.4.1048

    authors: Edelman L,Czarnecka E,Key JL

    更新日期:1988-04-01 00:00:00

  • The role of salicylic acid in the induction of cell death in Arabidopsis acd11.

    abstract::Salicylic acid (SA) is implicated in the induction of programmed cell death (PCD) associated with pathogen defense responses because SA levels increase in response to PCD-inducing infections, and PCD development can be inhibited by expression of salicylate hydroxylase encoded by the bacterial nahG gene. The acd11 muta...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.105.059303

    authors: Brodersen P,Malinovsky FG,Hématy K,Newman MA,Mundy J

    更新日期:2005-06-01 00:00:00

  • Beta-Amylases from Alfalfa (Medicago sativa L.) Roots.

    abstract::Amylase was found in high activity (193 international units per milligram protein) in the tap root of alfalfa (Medicago sativa L. cv. Sonora). The activity was separated by gel filtration chromatography into two fractions with molecular weights of 65,700 (heavy amylase) and 41,700 (light amylase). Activity staining of...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.69.5.1096

    authors: Doehlert DC,Duke SH,Anderson L

    更新日期:1982-05-01 00:00:00

  • The Mechanism of Abscisic Acid-induced Proline Accumulation in Barley Leaves.

    abstract::When leaf blades of fully expanded second leaves of barley (cv. Prior) were excised and incubated with the cut end in a 20 milligram per liter solution of abscisic acid, they accumulated proline at the rate of about 1 micromole per hour per gram fresh weight after a 3- to 4-hour lag. This accumulation occurred reprodu...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.66.2.230

    authors: Stewart CR

    更新日期:1980-08-01 00:00:00

  • Transcription Factor Arabidopsis Activating Factor1 Integrates Carbon Starvation Responses with Trehalose Metabolism.

    abstract::Plants respond to low carbon supply by massive reprogramming of the transcriptome and metabolome. We show here that the carbon starvation-induced NAC (for NO APICAL MERISTEM/ARABIDOPSIS TRANSCRIPTION ACTIVATION FACTOR/CUP-SHAPED COTYLEDON) transcription factor Arabidopsis (Arabidopsis thaliana) Transcription Activatio...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.15.00917

    authors: Garapati P,Feil R,Lunn JE,Van Dijck P,Balazadeh S,Mueller-Roeber B

    更新日期:2015-09-01 00:00:00

  • Ornithine decarboxylase, polyamines, and pyrrolizidine alkaloids in senecio and crotalaria.

    abstract::When tested for ornithine and arginine decarboxylases, pyrrolizidine alkaloid-bearing Senecio riddellii, S. longilobus (Compositae), and Crotalaria retusa (Leguminosae) plants exhibited only ornithine decarboxylase activity. This contrasts with previous studies of four species of pyrrolizidine alkaloid-bearing Heliotr...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.86.1.224

    authors: Birecka H,Birecki M,Cohen EJ,Bitonti AJ,McCann PP

    更新日期:1988-01-01 00:00:00