Sucrose phosphate synthase, a key enzyme for sucrose biosynthesis in plants: protein purification from corn leaves and immunological detection.

Abstract:

:We have purified the protein for the enzyme sucrose phosphate synthase (SPS) from corn (Zea mays) leaves. Partially purified SPS protein was used to generate specific monoclonal antibodies. The following immunoaffinity chromatography allowed the isolation of pure SPS protein. The apparent molecular mass of the SPS polypeptide is 138 kilodaltons. By immunoblot, an SPS antigen was found to accumulate in mature leaves. SPS protein levels remain constant during the day/night cycle. The observed diurnal fluctuation of extractable enzyme activity, therefore, must be caused by modification of the specific activity of SPS in vivo.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Bruneau JM,Worrell AC,Cambou B,Lando D,Voelker TA

doi

10.1104/pp.96.2.473

subject

Has Abstract

pub_date

1991-06-01 00:00:00

pages

473-8

issue

2

eissn

0032-0889

issn

1532-2548

journal_volume

96

pub_type

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