Abstract:
:Increasing the yield of oilseed crops is an important objective for biotechnologists. A number of individual genes involved in triacylglycerol metabolism have previously been reported to enhance the oil content of seeds when their expression is altered. However, it has yet to be established whether specific combinations of these genes can be used to achieve an additive effect and whether this leads to enhanced yield. Using Arabidopsis (Arabidopsis thaliana) as an experimental system, we show that seed-specific overexpression of WRINKLED1 (a transcriptional regulator of glycolysis and fatty acid synthesis) and DIACYLGLYCEROL ACYLTRANSFERASE1 (a triacylglycerol biosynthetic enzyme) combined with suppression of the triacylglycerol lipase SUGAR-DEPENDENT1 results in a higher percentage seed oil content and greater seed mass than manipulation of each gene individually. Analysis of total seed yield per plant suggests that, despite a reduction in seed number, the total yield of oil is also increased.
journal_name
Plant Physioljournal_title
Plant physiologyauthors
van Erp H,Kelly AA,Menard G,Eastmond PJdoi
10.1104/pp.114.236430subject
Has Abstractpub_date
2014-05-01 00:00:00pages
30-6issue
1eissn
0032-0889issn
1532-2548pii
pp.114.236430journal_volume
165pub_type
杂志文章相关文献
PLANT PHYSIOLOGY文献大全abstract::Highly purified fractions of three starch branching enzymes from developing maize (Zea mays L.) endosperm were used to prepare antisera in rabbits. In double diffusion experiments, no immunoprecipitate was observed when branching enzyme IIa or IIb was tested against branching enzyme I antiserum. No immunoprecipitate w...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.72.3.813
更新日期:1983-07-01 00:00:00
abstract::The activity of the alternative pathway is affected by a number of factors, including the level and reduction state of the alternative oxidase (AOX) protein, and the reduction state of the ubiquinone pool. To investigate the significance of these factors for the rate of alternative respiration in vivo, we studied root...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.126.1.376
更新日期:2001-05-01 00:00:00
abstract::Ethylene production and respiration by Granny Smith apples were inhibited by treatment with 20% CO(2) for 2 hours. A similar effect was observed in tissue slices when treated at either 0 or 25 degrees C.The inhibition continued even after an extended aeration period. There is also an inhibition of ethylene emission in...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.76.1.88
更新日期:1984-09-01 00:00:00
abstract::Poplar (Populus tremula x alba) trees (clone INRA 717-1-B4) were cultivated for 1 month in phytotronic chambers with two different levels of ozone (60 and 120 nL L(-1)). Foliar activities of shikimate dehydrogenase (EC 1.1.1.25), phenylalanine ammonia lyase (EC 4.3.1.5), and cinnamyl alcohol dehydrogenase (CAD, EC 1.1...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.103.031765
更新日期:2004-02-01 00:00:00
abstract::The eukaryotic genome is duplicated exactly once per cell division cycle. A strategy that limits every replication origin to a single initiation event is tightly regulated by a multiprotein complex, which involves at least 20 protein factors. A key player in this regulation is the evolutionary conserved hexameric MCM2...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.105.074294
更新日期:2006-02-01 00:00:00
abstract::Previous studies with isolated soybean cotyledon protoplasts revealed the presence of a saturable, simple diffusion, and nonsaturating carrier-mediated uptake of sucrose into soybean cotyledon cells. A proton/sucrose cotransport may be involved in the saturable sucrose uptake (Lin et al. 1984 Plant Physiol 75: 936-940...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.78.3.649
更新日期:1985-07-01 00:00:00
abstract::We have characterized the gravitropic response of inflorescence stems in Arabidopsis thaliana. When the inflorescence stems were placed horizontally, they curved upward about 90 degrees within 90 min in darkness at 23 degrees C, exhibiting strong negative gravitropism. Decapitated stem segments (without all flowers, f...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.110.3.933
更新日期:1996-03-01 00:00:00
abstract::Auxin-deprived, mannitol-supplemented, suspension-cultured pear (Pyrus communis L. Passe Crassane) fruit cells produce large quantities (20-40 nanoliters ethylene per 10(6) cells per hour) of ethylene in response to auxins, CuCl(2) or 1-amino-cyclopropane-1-carboxylic acid (ACC). Maximum rates of production are achiev...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.73.4.1013
更新日期:1983-12-01 00:00:00
abstract::At low water potential (psi(w)), dehydration reduces the symplast volume of leaf tissue. The effect of this reduction on photosynthetic capacity was investigated. The influence of osmotic adjustment on this relationship was also examined. To examine these relationships, comparative studies were undertaken on two wheat...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.85.4.1040
更新日期:1987-12-01 00:00:00
abstract::Root tips of many plant species release a number of border, or border-like, cells that are thought to play a major role in the protection of root meristem. However, little is currently known on the structure and function of the cell wall components of such root cells. Here, we investigate the sugar composition of the ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.112.198507
更新日期:2012-08-01 00:00:00
abstract::In pearl millet (Pennisetum americanum) seedlings, although the cytosolic phosphorylase was present in all organs, the plastidic phosphorylase was restricted to the leaf. Intercellular fractionation of the leaf revealed that cytosolic and plastidic phosphorylase were localized in the mesophyll and bundle-sheath cells,...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.109.2.517
更新日期:1995-10-01 00:00:00
abstract::A comparative study was made of the effects of auxin (alpha-naphthalene acetic acid), kinetin (6-furfurylaminopurine) and a mixture of auxin and kinetin applied in vivo on synthesis of RNA and protein and the distribution of such synthesis amongst the subcellular fractions of sections of endocarp from Kentucky Wonder ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.42.10.1334
更新日期:1967-10-01 00:00:00
abstract::Root iron mobilization genes are induced by iron deficiency downstream of an unknown signaling mechanism. The FER gene, encoding a basic helix-loop-helix domain protein and putative transcription factor, is required for induction of iron mobilization genes in roots of tomato (Lycopersicon esculentum). To study upstrea...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.104.054270
更新日期:2005-03-01 00:00:00
abstract::Maize (Zea mays L.) endosperm suspension cultures are a useful model system for studying biochemical and physiological events in developing maize endosperm. In this report, sugar uptake by the cultures is characterized. Uptake of (14)C-labeled fructose and l-glucose was linear with time, while the rate of uptake of ra...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.88.4.1235
更新日期:1988-12-01 00:00:00
abstract::Glyoxylate at a concentration of 10 millimolar caused 50% inhibition of decarboxylation of 20 millimolar [1-(14)C]glycine and accompanying synthesis of serine in a mitochondria-enriched preparation from tobacco (Nicotiana tabacum var. John Williams Broadleaf) leaves. None of the other compounds tested including format...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.70.1.61
更新日期:1982-07-01 00:00:00
abstract::In higher plants, three subfamilies of sucrose nonfermenting-1 (Snf1)-related protein kinases have evolved. While the Snf1-related protein kinase 1 (SnRK1) subfamily has been shown to share pivotal roles with the orthologous yeast Snf1 and mammalian AMP-activated protein kinase in modulating energy and metabolic homeo...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.109.150789
更新日期:2010-05-01 00:00:00
abstract::Dormancy is a complex evolutionary trait that temporally prevents seed germination, thus allowing seedling growth at a favorable season. High-throughput analyses of transcriptomes have led to significant progress in understanding the molecular regulation of this process, but the role of posttranscriptional mechanisms ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.15.00510
更新日期:2015-07-01 00:00:00
abstract::OsbZIP46 is one member of the third subfamily of bZIP transcription factors in rice (Oryza sativa). It has high sequence similarity to ABA-responsive element binding factor (ABF/AREB) transcription factors ABI5 and OsbZIP23, two transcriptional activators positively regulating stress tolerance in Arabidopsis (Arabidop...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.111.190389
更新日期:2012-04-01 00:00:00
abstract::Oleosins are unique and major proteins localized on the surface of oil bodies in diverse seed species. We purified five different oleosins (maize [Zea mays L.] KD 16 and KD 18, soybean [Glycine max L.] KD 18 and KD 24, and rapeseed [Brassica campestris L.] KD 20), and raised chicken antibodies against them. These anti...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.94.3.1282
更新日期:1990-11-01 00:00:00
abstract::Ribulose 1,5-bisphosphate carboxylase (RuBPCase) was chosen as a model protein to study how heat shock (HS) affects both chloroplast protein synthesis and the nuclear-chloroplast interaction in production of chloroplast proteins. Experiments were performed using highly chlorophyllous, soybean (Glycine max L. Merr. var...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.78.1.155
更新日期:1985-05-01 00:00:00
abstract::The influence of plant spacing and row orientation on spectral distribution of light received by growing soybean (Gylcine max [L.] Merr.) plants was measured under field conditions. Light absorption, reflection and transmission of individual leaves showed that most of the blue and red was absorbed while most of the fa...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.85.2.350
更新日期:1987-10-01 00:00:00
abstract::One of the two branches of the α-linolenic acid metabolism pathway is catalyzed by 12-oxo-phytodienoic acid reductase I, and the other is involved in jasmonic acid (JA) synthesis. The former is known to be active in the response to salinity tolerance in wheat (Triticum aestivum), but the participation of the latter in...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.113.227595
更新日期:2014-02-01 00:00:00
abstract::Transgenic Nicotiana plumbaginifolia plants that express either a 5-fold increase or a 20-fold decrease in nitrate reductase (NR) activity were used to study the relationships between carbon and nitrogen metabolism in leaves. Under saturating irradiance the maximum rate of photosynthesis, per unit surface area, was de...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.104.1.171
更新日期:1994-01-01 00:00:00
abstract::Previously the Arabidopsis (Arabidopsis thaliana) zinc finger protein OXIDATIVE STRESS2 (AtOXS2) and four OXS2-like (AtO2L) family members were described to play a role in stress tolerance and stress escape. For stress escape, SOC1 was a target of AtOXS2. However, for stress tolerance, the downstream targets were not ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.16.00220
更新日期:2016-07-01 00:00:00
abstract::When air-dry urediospores of the wheat stem rust, Puccinia graminis f. sp. tritici, are exposed to temperatures below freezing, their germinability is markedly reduced, even after prolonged thawing at room temperature. Germinability is fully restored by a brief heat-shock or by vapor phase hydration. We have found tha...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.47.2.289
更新日期:1971-02-01 00:00:00
abstract::Measurements of metabolic fluxes in whole embryos and isolated plastids have revealed major changes in the pathways of carbon utilization during cotyledon filling by oilseed rape (Brassica napus L.) embryos. In the early cotyledon stage (stage A), embryos used sucrose (Suc) predominantly for starch synthesis. Plastids...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.122.3.767
更新日期:2000-03-01 00:00:00
abstract::A wide diversity of isoprenoids is produced in different plant compartments. Most groups of isoprenoids synthesized in plastids, and some produced elsewhere in the plant cell derive from geranylgeranyl diphosphate (GGPP) synthesized by GGPP synthase (GGPPS) enzymes. In Arabidopsis (Arabidopsis thaliana), five genes ap...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.16.01392
更新日期:2016-11-01 00:00:00
abstract::Experiments with lettuce seeds during the first hours of imbibition showed that oxygen is necessary to sustain high adenine nucleotide ratios and consequently, energy charge values are higher than 0.8 as is usually the case in normally metabolizing tissues.The energy charge value (0.2) of dry seeds soaked in aerated w...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.68.3.631
更新日期:1981-09-01 00:00:00
abstract::A population of M2 seedlings of Arabidopsis thaliana was screened for mutants that were insensitive to the ethylene precursor 1-aminocyclopropane-1-carboxylate (ACC). Several independent lines were obtained and proved insensitive to both ACC and ethylene. Two lines were identified as alleles of a single recessive muta...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.102.2.401
更新日期:1993-06-01 00:00:00
abstract::Oleoresin (pitch) is a defensive secretion composed of monoterpene olefins (turpentine) and diterpene resin acids (rosin) that is produced in grand fir (Abies grandis Lindl.) stems in response to wounding. Monoterpene and diterpene biosynthesis are coordinately induced in wounded stems as determined by monitoring the ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.106.3.999
更新日期:1994-11-01 00:00:00