Multigene engineering of triacylglycerol metabolism boosts seed oil content in Arabidopsis.

Abstract:

:Increasing the yield of oilseed crops is an important objective for biotechnologists. A number of individual genes involved in triacylglycerol metabolism have previously been reported to enhance the oil content of seeds when their expression is altered. However, it has yet to be established whether specific combinations of these genes can be used to achieve an additive effect and whether this leads to enhanced yield. Using Arabidopsis (Arabidopsis thaliana) as an experimental system, we show that seed-specific overexpression of WRINKLED1 (a transcriptional regulator of glycolysis and fatty acid synthesis) and DIACYLGLYCEROL ACYLTRANSFERASE1 (a triacylglycerol biosynthetic enzyme) combined with suppression of the triacylglycerol lipase SUGAR-DEPENDENT1 results in a higher percentage seed oil content and greater seed mass than manipulation of each gene individually. Analysis of total seed yield per plant suggests that, despite a reduction in seed number, the total yield of oil is also increased.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

van Erp H,Kelly AA,Menard G,Eastmond PJ

doi

10.1104/pp.114.236430

subject

Has Abstract

pub_date

2014-05-01 00:00:00

pages

30-6

issue

1

eissn

0032-0889

issn

1532-2548

pii

pp.114.236430

journal_volume

165

pub_type

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