The Nature of Cold-induced Dormancy in Urediospores of Puccinia graminis tritici.

Abstract:

:When air-dry urediospores of the wheat stem rust, Puccinia graminis f. sp. tritici, are exposed to temperatures below freezing, their germinability is markedly reduced, even after prolonged thawing at room temperature. Germinability is fully restored by a brief heat-shock or by vapor phase hydration. We have found that this "cold dormancy" cannot be reversed once the spores contact liquid water. Enhanced loss of metabolites occurs immediately upon suspension of cold-dormant urediospores in liquid without a prior heat-shock. Such leakage is two to three times greater than from untreated or heatshocked cold-dormant spores and accounts for up to 70% of the soluble pool of metabolites normally present in germinating urediospores. Respiratory activity of cold-dormant urediospores declines rapidly during incubation in liquid. Incorporation of isotopic carbon into cold-dormant urediospores is only a fraction of that of untreated or heat-activated spores. Thus, cold shock transforms the spores into a state of supersensitivity to liquid water, which is reversed by heat-shock or slow hydration by vapor phase equilibration. The primary cause of damage to cold-dormant cells exposed to liquid water appears to be irreversible permeability damage, followed by metabolic injury.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Maheshwari R,Sussman AS

doi

10.1104/pp.47.2.289

subject

Has Abstract

pub_date

1971-02-01 00:00:00

pages

289-95

issue

2

eissn

0032-0889

issn

1532-2548

journal_volume

47

pub_type

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