Abstract:
:Ricin is a heterodimeric toxin that accumulates in the storage vacuoles of castor bean (Ricinus communis) endosperm. Proricin is synthesized as a single polypeptide precursor comprising the catalytic A chain and the Gal-binding B chain joined by a 12-amino acid linker propeptide. Upon arrival in the vacuole, the linker is removed. Here, we replicate these events in transfected tobacco (Nicotiana tabacum) leaf protoplasts. We show that the internal linker propeptide is responsible for vacuolar sorting and is sufficient to redirect the ricin heterodimer to the vacuole when fused to the A or the B chain. This internal peptide can also target two different secretory protein reporters to the vacuole. Moreover, mutation of the isoleucine residue within an NPIR-like motif of the propeptide affects vacuolar sorting in proricin and in the reconstituted A-B heterodimer. This is the first reported example of a sequence-specific vacuolar sorting signal located within an internal propeptide.
journal_name
Plant Physioljournal_title
Plant physiologyauthors
Frigerio L,Jolliffe NA,Di Cola A,Felipe DH,Paris N,Neuhaus JM,Lord JM,Ceriotti A,Roberts LMdoi
10.1104/pp.126.1.167subject
Has Abstractpub_date
2001-05-01 00:00:00pages
167-75issue
1eissn
0032-0889issn
1532-2548journal_volume
126pub_type
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