Abstract:
:Mung bean plants (Wilczek) accumulate increasingly greater amounts of buffer-extractable copper in both their shoots and roots when grown in liquid medium containing greater than 2 micrograms per milliliter copper (31.4 micromolar) as cupric sulfate. This increase in soluble copper is accompanied by an increase in the relative amount of low molecular weight (7,000 to 20,000) macromolecular-bound copper and a decrease in the relative amount of high molecular weight (greater than 20,000) copper. The major low molecular weight copper protein has been isolated from copper-intoxicated mung bean plants by a combination of ammonium sulfate fractionation, gel filtration, and ion exchange chromatography. It was identified as mung bean plastocyanin on the basis of its molecular weight, optical behavior, and amino acid composition. No evidence was found for a low molecular weight copper-binding protein corresponding to mammalian thionein or chelatin.
journal_name
Plant Physioljournal_title
Plant physiologyauthors
Nicholson C,Stein J,Wilson KAdoi
10.1104/pp.66.2.272subject
Has Abstractpub_date
1980-08-01 00:00:00pages
272-5issue
2eissn
0032-0889issn
1532-2548journal_volume
66pub_type
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