Identification of the Low Molecular Weight Copper Protein from Copper-intoxicated Mung Bean Plants.

Abstract:

:Mung bean plants (Wilczek) accumulate increasingly greater amounts of buffer-extractable copper in both their shoots and roots when grown in liquid medium containing greater than 2 micrograms per milliliter copper (31.4 micromolar) as cupric sulfate. This increase in soluble copper is accompanied by an increase in the relative amount of low molecular weight (7,000 to 20,000) macromolecular-bound copper and a decrease in the relative amount of high molecular weight (greater than 20,000) copper. The major low molecular weight copper protein has been isolated from copper-intoxicated mung bean plants by a combination of ammonium sulfate fractionation, gel filtration, and ion exchange chromatography. It was identified as mung bean plastocyanin on the basis of its molecular weight, optical behavior, and amino acid composition. No evidence was found for a low molecular weight copper-binding protein corresponding to mammalian thionein or chelatin.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Nicholson C,Stein J,Wilson KA

doi

10.1104/pp.66.2.272

subject

Has Abstract

pub_date

1980-08-01 00:00:00

pages

272-5

issue

2

eissn

0032-0889

issn

1532-2548

journal_volume

66

pub_type

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