Abstract:
:Experiments in which chloroplasts were washed with tris and tricine buffers at different pH's indicated that the non-protonated (uncharged) form of tris was inhibitory to the Hill reaction while the protonated form of tris and the zwitterionic forms of tricine were non-inhibitory. Buffers analogous to tris and tricine gave similar results. Photoreduction of NADP could be restored to the inhibited chloroplasts by adding the reduced forms of p-hydroquinone, p-aminophenol, p-phenylenediamine, benzidine, semicarbazide, and dihydroxydiphenyl, all of which donated electrons to photosystem II. Photoreduction of ferricyanide was shown with those donor systems (benzidine and semicarbazide) which did not react chemically with ferricyanide. Photophosphorylation was also restored with all of the electron donors except semicarbazide.
journal_name
Plant Physioljournal_title
Plant physiologyauthors
Yamashita T,Butler WLdoi
10.1104/pp.44.3.435subject
Has Abstractpub_date
1969-03-01 00:00:00pages
435-8issue
3eissn
0032-0889issn
1532-2548journal_volume
44pub_type
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