Inactivation of pea leaf chloroplastic and cytoplasmic glucose 6-phosphate dehydrogenases by light and dithiothreitol.

Abstract:

:Chloroplastic and cytoplasmic forms of pea (Pisum sativum L.) leaf glucose-6-P dehydrogenase can be separated by gel electrophoresis. Both forms are found in etiolated seedlings. When light-grown plants are illuminated or when crude extracts are treated with dithiothreitol, the chloroplastic and cytoplasmic forms of this enzyme are inactivated. Michaelis-Menten kinetics are obtained for inactivation of the chloroplastic and cytoplasmic glucose-6-P dehydrogenases and for activation of two dehydrogenases involved in photosynthetic carbon metabolism with dithiothreitol. The mechanism for the activation of the two light-activated enzymes appears to be similar to the mechanism for the inactivation of the chloroplastic and cytoplasmic forms of the light-inactivated oxidative pentose phosphate enzyme glucose-6-P dehydrogenase.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Anderson LE,Ng TC,Park KE

doi

10.1104/pp.53.6.835

subject

Has Abstract

pub_date

1974-06-01 00:00:00

pages

835-9

issue

6

eissn

0032-0889

issn

1532-2548

journal_volume

53

pub_type

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