Abstract:
:Chloroplastic and cytoplasmic forms of pea (Pisum sativum L.) leaf glucose-6-P dehydrogenase can be separated by gel electrophoresis. Both forms are found in etiolated seedlings. When light-grown plants are illuminated or when crude extracts are treated with dithiothreitol, the chloroplastic and cytoplasmic forms of this enzyme are inactivated. Michaelis-Menten kinetics are obtained for inactivation of the chloroplastic and cytoplasmic glucose-6-P dehydrogenases and for activation of two dehydrogenases involved in photosynthetic carbon metabolism with dithiothreitol. The mechanism for the activation of the two light-activated enzymes appears to be similar to the mechanism for the inactivation of the chloroplastic and cytoplasmic forms of the light-inactivated oxidative pentose phosphate enzyme glucose-6-P dehydrogenase.
journal_name
Plant Physioljournal_title
Plant physiologyauthors
Anderson LE,Ng TC,Park KEdoi
10.1104/pp.53.6.835subject
Has Abstractpub_date
1974-06-01 00:00:00pages
835-9issue
6eissn
0032-0889issn
1532-2548journal_volume
53pub_type
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