State transitions in Chlamydomonas reinhardtii. The role of the Mehler reaction in state 2-to-state 1 transition.

Abstract:

:The light intensity-dependent transition to state 1 of dark-adapted anaerobic state 2 Chlamydomonas reinhardtii cells is stimulated by oxygen and by other electron acceptors for photosystem I, such as oxaloacetate and methylviologen. This suggests that the transition to state 1 requires the oxidation of the intersystem chain by photosystem I photochemistry. On the other hand, the mere oxidation in the dark of the chain-by addition of O2-leads only to a slow and incomplete transition. The light-driven stimulation by O2 of the state 1 transition is saturated at an O2 concentration of 15 to 20 microm, definitely higher than that of respiration. We suggest that this may represent the affinity for oxygen of the Mehler reaction, a conclusion that is confirmed by the observations that mitochondrial respiration is apparently not involved in modulating state 2-to-state 1 transition. The catalysis of the state 2-to-state 1 transition upon illumination of anaerobically adapted algae might represent, therefore, a relevant physiological role of this process in C. reinhardtii.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Forti G,Caldiroli G

doi

10.1104/pp.104.048256

subject

Has Abstract

pub_date

2005-02-01 00:00:00

pages

492-9

issue

2

eissn

0032-0889

issn

1532-2548

pii

pp.104.048256

journal_volume

137

pub_type

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